TY  - JOUR
AU  - Kalauzi, Aleksandar
AU  - Nikolić, Ljiljana
AU  - Savić, Danijela
AU  - Radotic, Ksenija
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2011
AB  - The physiological and biochemical factors that lead to cell death have
   not been recognized completely. To our knowledge, there are no data on
   the bioelectric parameters that characterize early period of cell death,
   as well as on the appearance of related membrane current frequencies. We
   studied early parameters of glutaraldehyde (GA)-induced cell death, by
   examining the membrane properties of mouse microglia using the
   whole-cell patch-clamp technique. In addition, we investigated the
   GA-induced changes in the membrane current frequency, to see if
   characteristic frequencies would appear in dying cell. For data
   analysis, we applied a new approach, an improved multiple moving window
   length analysis and interval weighted spectra averaging (IWSA). We chose
   GA for its ability to induce almost instantaneous cell death. The 0.6 \%
   GA did not induce changes in the bioelectric membrane properties of
   microglia. However, the 3 \% GA caused significant decrease of membrane
   capacitance and resistance accompanied by the prominent increase in the
   membrane currents and nearly ohmic current response of microglial cells.
   These data indicate that 3 \% GA caused complete loss of the membrane
   function consequently inducing instantaneous cell death. The membrane
   function loss was characterized by appearance of the 1.26-4.62 Hz
   frequency peak in the IWSA spectra, while no significant increase of
   amplitudes could be observed for cells treated with 0.6 \% GA. To our
   knowledge, this is the first record of a frequency associated with
   complete loss of the membrane function and thus can be considered as an
   early indicator of cell death.
T2  - Journal of Membrane Biology
T1  - Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval
 Weighted Spectra Averaging: Changes in Membrane Properties and Current
 Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde
IS  - 1
VL  - 248
DO  - 10.1007/s00232-014-9748-7
SP  - 117
EP  - 123
ER  - 

@article{
author = "Kalauzi, Aleksandar and Nikolić, Ljiljana and Savić, Danijela and Radotic, Ksenija",
year = "2015",
abstract = "The physiological and biochemical factors that lead to cell death have
   not been recognized completely. To our knowledge, there are no data on
   the bioelectric parameters that characterize early period of cell death,
   as well as on the appearance of related membrane current frequencies. We
   studied early parameters of glutaraldehyde (GA)-induced cell death, by
   examining the membrane properties of mouse microglia using the
   whole-cell patch-clamp technique. In addition, we investigated the
   GA-induced changes in the membrane current frequency, to see if
   characteristic frequencies would appear in dying cell. For data
   analysis, we applied a new approach, an improved multiple moving window
   length analysis and interval weighted spectra averaging (IWSA). We chose
   GA for its ability to induce almost instantaneous cell death. The 0.6 \%
   GA did not induce changes in the bioelectric membrane properties of
   microglia. However, the 3 \% GA caused significant decrease of membrane
   capacitance and resistance accompanied by the prominent increase in the
   membrane currents and nearly ohmic current response of microglial cells.
   These data indicate that 3 \% GA caused complete loss of the membrane
   function consequently inducing instantaneous cell death. The membrane
   function loss was characterized by appearance of the 1.26-4.62 Hz
   frequency peak in the IWSA spectra, while no significant increase of
   amplitudes could be observed for cells treated with 0.6 \% GA. To our
   knowledge, this is the first record of a frequency associated with
   complete loss of the membrane function and thus can be considered as an
   early indicator of cell death.",
journal = "Journal of Membrane Biology",
title = "Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval
 Weighted Spectra Averaging: Changes in Membrane Properties and Current
 Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde",
number = "1",
volume = "248",
doi = "10.1007/s00232-014-9748-7",
pages = "117-123"
}

Kalauzi, A., Nikolić, L., Savić, D.,& Radotic, K.. (2015). Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval
 Weighted Spectra Averaging: Changes in Membrane Properties and Current
 Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde. in Journal of Membrane Biology, 248(1), 117-123.
https://doi.org/10.1007/s00232-014-9748-7

Kalauzi A, Nikolić L, Savić D, Radotic K. Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval
 Weighted Spectra Averaging: Changes in Membrane Properties and Current
 Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde. in Journal of Membrane Biology. 2015;248(1):117-123.
doi:10.1007/s00232-014-9748-7 .

Kalauzi, Aleksandar, Nikolić, Ljiljana, Savić, Danijela, Radotic, Ksenija, "Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval
 Weighted Spectra Averaging: Changes in Membrane Properties and Current
 Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde" in Journal of Membrane Biology, 248, no. 1 (2015):117-123,
https://doi.org/10.1007/s00232-014-9748-7 . .

TY  - JOUR
AU  - Pešić, Milica
AU  - Podolski-Renić, Ana
AU  - Stojković Burić, Sonja
AU  - Matovic, Branko
AU  - Zmejkoski, Danica
AU  - Kojic, Vesna
AU  - Bogdanovic, Gordana
AU  - Pavicevic, Aleksandra
AU  - Mojovic, Milos
AU  - Savic, Aleksandar
AU  - Milenkovic, Ivana
AU  - Kalauzi, Aleksandar
AU  - Radotic, Ksenija
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1957
AB  - Data on medical applications of cerium oxide nanoparticles CeO2 (CONP)
   are promising, yet information regarding their action in cells is
   incomplete and there are conflicting reports about in vitro toxicity.
   Herein, we have studied cytotoxic effect of CONP in several cancer and
   normal cell lines and their potential to change intracellular redox
   status. The IC50 was achieved only in two of eight tested cell lines,
   melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating
   room temperature method was applied to produce CONP with an average
   crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2-
   vacancies. The induction of cell death by CONP and the production of
   reactive oxygen species (ROS) were analyzed by flow-cytometry. Free
   radicals related antioxidant capacity of the cells was studied by the
   reduction of stable free radical TEMPONE using electron spin resonance
   spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell
   lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small
   cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while
   normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5)
   were insensitive. The most sensitive were 518A2 melanoma and HT-29
   colorectal adenocarcinoma cell lines, with the IC50 values being between
   100 and 200 mu M. Decreased rate of TEMPONE reduction and increased
   production of certain ROS species (peroxynitrite and hydrogen peroxide
   anion) indicates that free radical metabolism, thus redox status was
   changed, and antioxidant capacity damaged in the CONP treated 518A2 and
   HT-29 cells. In conclusion, changes in intracellular redox status
   induced by CONP are partly attributed to the prooxidant activity of the
   nanoparticles. Further, ROS induced cell damages might eventually lead
   to the cell death. However, low inhibitory potential of CONP in the
   other human cell lines tested indicates that CONP may be safe for human
   usage in industry and medicine. (C) 2015 Elsevier Ireland Ltd. All
   rights reserved.
T2  - Chemico-Biological Interactions
T1  - Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity
VL  - 232
DO  - 10.1016/j.cbi.2015.03.013
SP  - 85
EP  - 93
ER  - 

@article{
author = "Pešić, Milica and Podolski-Renić, Ana and Stojković Burić, Sonja and Matovic, Branko and Zmejkoski, Danica and Kojic, Vesna and Bogdanovic, Gordana and Pavicevic, Aleksandra and Mojovic, Milos and Savic, Aleksandar and Milenkovic, Ivana and Kalauzi, Aleksandar and Radotic, Ksenija",
year = "2015",
abstract = "Data on medical applications of cerium oxide nanoparticles CeO2 (CONP)
   are promising, yet information regarding their action in cells is
   incomplete and there are conflicting reports about in vitro toxicity.
   Herein, we have studied cytotoxic effect of CONP in several cancer and
   normal cell lines and their potential to change intracellular redox
   status. The IC50 was achieved only in two of eight tested cell lines,
   melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating
   room temperature method was applied to produce CONP with an average
   crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2-
   vacancies. The induction of cell death by CONP and the production of
   reactive oxygen species (ROS) were analyzed by flow-cytometry. Free
   radicals related antioxidant capacity of the cells was studied by the
   reduction of stable free radical TEMPONE using electron spin resonance
   spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell
   lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small
   cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while
   normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5)
   were insensitive. The most sensitive were 518A2 melanoma and HT-29
   colorectal adenocarcinoma cell lines, with the IC50 values being between
   100 and 200 mu M. Decreased rate of TEMPONE reduction and increased
   production of certain ROS species (peroxynitrite and hydrogen peroxide
   anion) indicates that free radical metabolism, thus redox status was
   changed, and antioxidant capacity damaged in the CONP treated 518A2 and
   HT-29 cells. In conclusion, changes in intracellular redox status
   induced by CONP are partly attributed to the prooxidant activity of the
   nanoparticles. Further, ROS induced cell damages might eventually lead
   to the cell death. However, low inhibitory potential of CONP in the
   other human cell lines tested indicates that CONP may be safe for human
   usage in industry and medicine. (C) 2015 Elsevier Ireland Ltd. All
   rights reserved.",
journal = "Chemico-Biological Interactions",
title = "Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity",
volume = "232",
doi = "10.1016/j.cbi.2015.03.013",
pages = "85-93"
}

Pešić, M., Podolski-Renić, A., Stojković Burić, S., Matovic, B., Zmejkoski, D., Kojic, V., Bogdanovic, G., Pavicevic, A., Mojovic, M., Savic, A., Milenkovic, I., Kalauzi, A.,& Radotic, K.. (2015). Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity. in Chemico-Biological Interactions, 232, 85-93.
https://doi.org/10.1016/j.cbi.2015.03.013

Pešić M, Podolski-Renić A, Stojković Burić S, Matovic B, Zmejkoski D, Kojic V, Bogdanovic G, Pavicevic A, Mojovic M, Savic A, Milenkovic I, Kalauzi A, Radotic K. Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity. in Chemico-Biological Interactions. 2015;232:85-93.
doi:10.1016/j.cbi.2015.03.013 .

Pešić, Milica, Podolski-Renić, Ana, Stojković Burić, Sonja, Matovic, Branko, Zmejkoski, Danica, Kojic, Vesna, Bogdanovic, Gordana, Pavicevic, Aleksandra, Mojovic, Milos, Savic, Aleksandar, Milenkovic, Ivana, Kalauzi, Aleksandar, Radotic, Ksenija, "Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity" in Chemico-Biological Interactions, 232 (2015):85-93,
https://doi.org/10.1016/j.cbi.2015.03.013 . .