TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Malbeck, Jiří
AU  - Dobrev, Petre I.
AU  - Zdravković-Korać, Snežana
AU  - Simonović, Ana
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Zahajská, Lenka
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3605
AB  - Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.
T2  - Journal of Plant Growth Regulation
T1  - Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization
VL  - 40
DO  - 10.1007/s00344-020-10080-w
SP  - 37
EP  - 47
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Malbeck, Jiří and Dobrev, Petre I. and Zdravković-Korać, Snežana and Simonović, Ana and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Zahajská, Lenka and Tadić, Vojin and Dragićević, Ivana",
year = "2021",
abstract = "Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.",
journal = "Journal of Plant Growth Regulation",
title = "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization",
volume = "40",
doi = "10.1007/s00344-020-10080-w",
pages = "37-47"
}

Raspor, M., Motyka, V., Ninković, S., Malbeck, J., Dobrev, P. I., Zdravković-Korać, S., Simonović, A., Ćosić, T., Cingel, A., Savić, J., Zahajská, L., Tadić, V.,& Dragićević, I.. (2021). Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation, 40, 37-47.
https://doi.org/10.1007/s00344-020-10080-w

Raspor M, Motyka V, Ninković S, Malbeck J, Dobrev PI, Zdravković-Korać S, Simonović A, Ćosić T, Cingel A, Savić J, Zahajská L, Tadić V, Dragićević I. Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation. 2021;40:37-47.
doi:10.1007/s00344-020-10080-w .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Malbeck, Jiří, Dobrev, Petre I., Zdravković-Korać, Snežana, Simonović, Ana, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Zahajská, Lenka, Tadić, Vojin, Dragićević, Ivana, "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization" in Journal of Plant Growth Regulation, 40 (2021):37-47,
https://doi.org/10.1007/s00344-020-10080-w . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Dobrev, Petre I.
AU  - Malbeck, Jiří
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2020
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7044434
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3623
AB  - A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.
T2  - Scientific Reports
T1  - Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-60412-9
SP  - 3437
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Dobrev, Petre I. and Malbeck, Jiří and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Tadić, Vojin and Dragićević, Ivana",
year = "2020",
abstract = "A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.",
journal = "Scientific Reports",
title = "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-60412-9",
pages = "3437"
}

Raspor, M., Motyka, V., Ninković, S., Dobrev, P. I., Malbeck, J., Ćosić, T., Cingel, A., Savić, J., Tadić, V.,& Dragićević, I.. (2020). Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports, 10(1), 3437.
https://doi.org/10.1038/s41598-020-60412-9

Raspor M, Motyka V, Ninković S, Dobrev PI, Malbeck J, Ćosić T, Cingel A, Savić J, Tadić V, Dragićević I. Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports. 2020;10(1):3437.
doi:10.1038/s41598-020-60412-9 .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Dobrev, Petre I., Malbeck, Jiří, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Tadić, Vojin, Dragićević, Ivana, "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics." in Scientific Reports, 10, no. 1 (2020):3437,
https://doi.org/10.1038/s41598-020-60412-9 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Václav
AU  - Dragićević, Ivana C.
AU  - Petrić, Marija
AU  - Jevremović, Slađana
AU  - Malbeck, Jiří
AU  - Holik, Josef
AU  - Dobrev, Petre I.
AU  - Subotić, Angelina
PY  - 2016
UR  - https://link.springer.com/article/10.1007%2Fs00344-015-9558-x
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3381
AB  - Phytohormones are important regulators of
numerous developmental and physiological processes in
plants. Spontaneous morphogenesis of the common centaury
(Centaurium erythraea Rafn.) is possible on nutrition
medium without addition of any plant growth
regulator depending solely on endogenous phytohormone
levels. Thus, this plant species represents a very good
model system for the investigation of numerous physiological
processes under phytohormonal control in vitro.
We analysed the total amount of endogenous cytokinins
(CKs) including the contents of their individual groups in
shoots and roots of C. erythraea plants grown in vitro. The
total amount of endogenous CKs was 1.4 times higher in
shoots than in roots. Inactive or weakly active N-glucosides
found to predominate in both organs of centaury
plants, whereas free bases and O-glucosides represented
only a small portion of the total CK pool. Consequently,
centaury roots showed higher IAA content as well as IAA/
free CK base ratios compared to shoots. Centaury tissues
also showed increased levels of ‘‘stress hormones’’. In
contrast to SA, considerably higher levels of ABA were
found in centaury shoots than in roots. Our results could
serve as a basis for understanding and elucidating spontaneous
de novo shoot organogenesis and further plant
regeneration of C. erythraea in vitro.
T2  - Journal of Plant Growth Regulation
T1  - Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro
IS  - 2
VL  - 35
DO  - 10.1007/s00344-015-9558-x
SP  - 543
EP  - 552
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Motyka, Václav and Dragićević, Ivana C. and Petrić, Marija and Jevremović, Slađana and Malbeck, Jiří and Holik, Josef and Dobrev, Petre I. and Subotić, Angelina",
year = "2016",
abstract = "Phytohormones are important regulators of
numerous developmental and physiological processes in
plants. Spontaneous morphogenesis of the common centaury
(Centaurium erythraea Rafn.) is possible on nutrition
medium without addition of any plant growth
regulator depending solely on endogenous phytohormone
levels. Thus, this plant species represents a very good
model system for the investigation of numerous physiological
processes under phytohormonal control in vitro.
We analysed the total amount of endogenous cytokinins
(CKs) including the contents of their individual groups in
shoots and roots of C. erythraea plants grown in vitro. The
total amount of endogenous CKs was 1.4 times higher in
shoots than in roots. Inactive or weakly active N-glucosides
found to predominate in both organs of centaury
plants, whereas free bases and O-glucosides represented
only a small portion of the total CK pool. Consequently,
centaury roots showed higher IAA content as well as IAA/
free CK base ratios compared to shoots. Centaury tissues
also showed increased levels of ‘‘stress hormones’’. In
contrast to SA, considerably higher levels of ABA were
found in centaury shoots than in roots. Our results could
serve as a basis for understanding and elucidating spontaneous
de novo shoot organogenesis and further plant
regeneration of C. erythraea in vitro.",
journal = "Journal of Plant Growth Regulation",
title = "Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro",
number = "2",
volume = "35",
doi = "10.1007/s00344-015-9558-x",
pages = "543-552"
}

Trifunović-Momčilov, M., Motyka, V., Dragićević, I. C., Petrić, M., Jevremović, S., Malbeck, J., Holik, J., Dobrev, P. I.,& Subotić, A.. (2016). Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro. in Journal of Plant Growth Regulation, 35(2), 543-552.
https://doi.org/10.1007/s00344-015-9558-x

Trifunović-Momčilov M, Motyka V, Dragićević IC, Petrić M, Jevremović S, Malbeck J, Holik J, Dobrev PI, Subotić A. Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro. in Journal of Plant Growth Regulation. 2016;35(2):543-552.
doi:10.1007/s00344-015-9558-x .

Trifunović-Momčilov, Milana, Motyka, Václav, Dragićević, Ivana C., Petrić, Marija, Jevremović, Slađana, Malbeck, Jiří, Holik, Josef, Dobrev, Petre I., Subotić, Angelina, "Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro" in Journal of Plant Growth Regulation, 35, no. 2 (2016):543-552,
https://doi.org/10.1007/s00344-015-9558-x . .