TY  - JOUR
AU  - Napar, Wado Photo Faqir
AU  - Kaleri, Abdul Rasheed
AU  - Ahmed, Awais
AU  - Nabi, Farhan
AU  - Sajid, Sumbal
AU  - Ćosić, Tatjana
AU  - Yao, Yinan
AU  - Liu, Jikai
AU  - Raspor, Martin
AU  - Gao, Yongfeng
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4869
AB  - Tomato cultivation is affected by high soil salinity and drought stress, which cause major yield losses worldwide. In this work, we compare the efficiency of mechanisms of tolerance to salinity, and osmotic stress applied as mannitol or drought, in three tomato genotypes: LA-2838 (Ailsa Craig), LA-2662 (Saladette), and LA-1996 (Anthocyanin  fruit  – Aft),  a  genotype  known  for  high  anthocyanin  content. Exposure  to  salinity  or   drought induced  stress  in  all three  genotypes,  but the  LA-1996 plants displayed  superior tolerance to stress compared with the other two genotypes. They were more efficient in anthocyanin and proline accumulation, superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activity, and leaf Na+, K+, and Ca2+ homeostasis. In addition, they suffered lesser oxidative damage as measured by chlorophyll (Chl) loss and malondialdehyde (MDA) accumulation, and bioassays showed that they were less affected in terms of seed germination and root elongation. Exposure to stress induced the upregulation of stress-related genes SlNCED1, SlAREB1, SlABF4, SlWRKY8, and SlDREB2A more efficiently in LA-1996 than in the two susceptible genotypes. Conversely, the upregulation of the NADPH oxidase gene SlRBOH1 was more pronounced in LA-2838 and LA-2662. Principal component analysis showed obvious distinction between the tolerant genotype LA-1996 and the susceptible LA- 2838 and LA-2662 in response to stress, and association of leaf and stem anthocyanin content with major stress tolerance traits. We suggest that anthocyanin accumulation can be considered as a marker of stress tolerance in tomato, and that LA-1996 can be considered for cultivation in salinity- or drought-affected areas.
PB  - Elsevier GmbH
T2  - Journal of Plant Physiology
T1  - The anthocyanin-rich tomato genotype LA-1996 displays superior efficiency of mechanisms of tolerance to salinity and drought
IS  - 271
DO  - 10.1016/j.jplph.2022.153662
SP  - 153662
ER  - 

@article{
author = "Napar, Wado Photo Faqir and Kaleri, Abdul Rasheed and Ahmed, Awais and Nabi, Farhan and Sajid, Sumbal and Ćosić, Tatjana and Yao, Yinan and Liu, Jikai and Raspor, Martin and Gao, Yongfeng",
year = "2022",
abstract = "Tomato cultivation is affected by high soil salinity and drought stress, which cause major yield losses worldwide. In this work, we compare the efficiency of mechanisms of tolerance to salinity, and osmotic stress applied as mannitol or drought, in three tomato genotypes: LA-2838 (Ailsa Craig), LA-2662 (Saladette), and LA-1996 (Anthocyanin  fruit  – Aft),  a  genotype  known  for  high  anthocyanin  content. Exposure  to  salinity  or   drought induced  stress  in  all three  genotypes,  but the  LA-1996 plants displayed  superior tolerance to stress compared with the other two genotypes. They were more efficient in anthocyanin and proline accumulation, superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activity, and leaf Na+, K+, and Ca2+ homeostasis. In addition, they suffered lesser oxidative damage as measured by chlorophyll (Chl) loss and malondialdehyde (MDA) accumulation, and bioassays showed that they were less affected in terms of seed germination and root elongation. Exposure to stress induced the upregulation of stress-related genes SlNCED1, SlAREB1, SlABF4, SlWRKY8, and SlDREB2A more efficiently in LA-1996 than in the two susceptible genotypes. Conversely, the upregulation of the NADPH oxidase gene SlRBOH1 was more pronounced in LA-2838 and LA-2662. Principal component analysis showed obvious distinction between the tolerant genotype LA-1996 and the susceptible LA- 2838 and LA-2662 in response to stress, and association of leaf and stem anthocyanin content with major stress tolerance traits. We suggest that anthocyanin accumulation can be considered as a marker of stress tolerance in tomato, and that LA-1996 can be considered for cultivation in salinity- or drought-affected areas.",
publisher = "Elsevier GmbH",
journal = "Journal of Plant Physiology",
title = "The anthocyanin-rich tomato genotype LA-1996 displays superior efficiency of mechanisms of tolerance to salinity and drought",
number = "271",
doi = "10.1016/j.jplph.2022.153662",
pages = "153662"
}

Napar, W. P. F., Kaleri, A. R., Ahmed, A., Nabi, F., Sajid, S., Ćosić, T., Yao, Y., Liu, J., Raspor, M.,& Gao, Y.. (2022). The anthocyanin-rich tomato genotype LA-1996 displays superior efficiency of mechanisms of tolerance to salinity and drought. in Journal of Plant Physiology
Elsevier GmbH.(271), 153662.
https://doi.org/10.1016/j.jplph.2022.153662

Napar WPF, Kaleri AR, Ahmed A, Nabi F, Sajid S, Ćosić T, Yao Y, Liu J, Raspor M, Gao Y. The anthocyanin-rich tomato genotype LA-1996 displays superior efficiency of mechanisms of tolerance to salinity and drought. in Journal of Plant Physiology. 2022;(271):153662.
doi:10.1016/j.jplph.2022.153662 .

Napar, Wado Photo Faqir, Kaleri, Abdul Rasheed, Ahmed, Awais, Nabi, Farhan, Sajid, Sumbal, Ćosić, Tatjana, Yao, Yinan, Liu, Jikai, Raspor, Martin, Gao, Yongfeng, "The anthocyanin-rich tomato genotype LA-1996 displays superior efficiency of mechanisms of tolerance to salinity and drought" in Journal of Plant Physiology, no. 271 (2022):153662,
https://doi.org/10.1016/j.jplph.2022.153662 . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Kaleri, Abdul Rasheed
AU  - Ninković, Slavica
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ćosić, Tatjana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4303
AB  - De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro
regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media
supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this
process, and genes involved in their signaling cascades act as master regulators of the different phases
of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in
detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration
are often interpreted as a consequence of these hormones simply being present in the regeneration
media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are
generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon
source, plays a signaling role and has been recently shown to interact with CK and auxin and to
affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation
of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the
regeneration media and their interaction with sucrose present in the media to their complex signaling
outputs that mediate shoot regeneration.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs
IS  - 16
VL  - 22
DO  - 10.3390/ijms22168554
SP  - 8554
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Kaleri, Abdul Rasheed and Ninković, Slavica and Tubić, Ljiljana and Cingel, Aleksandar and Ćosić, Tatjana",
year = "2021",
abstract = "De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro
regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media
supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this
process, and genes involved in their signaling cascades act as master regulators of the different phases
of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in
detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration
are often interpreted as a consequence of these hormones simply being present in the regeneration
media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are
generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon
source, plays a signaling role and has been recently shown to interact with CK and auxin and to
affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation
of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the
regeneration media and their interaction with sucrose present in the media to their complex signaling
outputs that mediate shoot regeneration.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs",
number = "16",
volume = "22",
doi = "10.3390/ijms22168554",
pages = "8554"
}

Raspor, M., Motyka, V., Kaleri, A. R., Ninković, S., Tubić, L., Cingel, A.,& Ćosić, T.. (2021). Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. in International Journal of Molecular Sciences
Basel: MDPI., 22(16), 8554.
https://doi.org/10.3390/ijms22168554

Raspor M, Motyka V, Kaleri AR, Ninković S, Tubić L, Cingel A, Ćosić T. Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. in International Journal of Molecular Sciences. 2021;22(16):8554.
doi:10.3390/ijms22168554 .

Raspor, Martin, Motyka, Václav, Kaleri, Abdul Rasheed, Ninković, Slavica, Tubić, Ljiljana, Cingel, Aleksandar, Ćosić, Tatjana, "Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs" in International Journal of Molecular Sciences, 22, no. 16 (2021):8554,
https://doi.org/10.3390/ijms22168554 . .

TY  - JOUR
AU  - Kaleri, Abdul Rasheed
AU  - Ma, Jiahua
AU  - Abro, Saeed Akhtar
AU  - Faqir, Yahya
AU  - Nabi, Farhan
AU  - Hakeem, Abdul
AU  - Ahmed, Awais
AU  - Ahmed, Siraj
AU  - Jakhar, Ali Murad
AU  - Shah, Sayed Mustajab
AU  - Tan, Chengjia
AU  - Qing, Yuxin
AU  - Raspor, Martin
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4373
AB  - Dung beetles have been used to enhance soil health and crop growth and yields, but their effects on
soil enzymology and microbial diversity are seldom reported. The present work reports on beneficial
effects of dung beetles on soil enzyme activity, bacterial diversity and growth and antioxidant content
of Chinese cabbage (Brassica rapa ssp. pekinensis). The soil amended with cow dung only (CD), or
with dung broken down by dung beetles (DB), was compared with untreated control (CO). Soil
bacterial diversity was determined through 16S rRNA gene sequencing, and enzymological tests were
used for soil enzyme activity. Dung beetles significantly increased acid phosphatase, urease,
cellulase, catalase and β-glucosidase activities in the soil compared with CD or control. Bacterial
alpha-diversity significantly increased in DB when compared with CD or control. Microbial carbon
(C) and nitrogen (N) were significantly higher in DB than in CD and in CD than control. The DB and
CD treatments also significantly enhanced plant growth and the content of antioxidants (total
flavonoids, polyphenols and vitamin C) and phytohormones indole-3-acetic acid (IAA), trans-zeatin
riboside (ZR) and gibberellic acid (GA3) compared with control, while a significant reduction was
noted in abscisic acid (ABA) in DB compared with CD or control plants. Correlation analysis
confirmed that the enzyme activities are pH dependent, as pH was significantly lowered by DB
compared with CD or control. Our results suggest that use of dung beetles in conventional practices
could be an effective strategy to improve soil nutrient availability and microbial activity and
antioxidant content of Chinese cabbage.
PB  - Springer
T2  - Journal of Soil Science and Plant Nutrition
T1  - Dung Beetle Improves soil Bacterial Diversity and Enzyme Activity and Enhances Growth and  Antioxidant Content of Chinese Cabbage (Brassica rapa ssp. pekinensis)
IS  - 4
VL  - 21
DO  - 10.1007/s42729-021-00614-w
SP  - 3387
EP  - 3401
ER  - 

@article{
author = "Kaleri, Abdul Rasheed and Ma, Jiahua and Abro, Saeed Akhtar and Faqir, Yahya and Nabi, Farhan and Hakeem, Abdul and Ahmed, Awais and Ahmed, Siraj and Jakhar, Ali Murad and Shah, Sayed Mustajab and Tan, Chengjia and Qing, Yuxin and Raspor, Martin",
year = "2021",
abstract = "Dung beetles have been used to enhance soil health and crop growth and yields, but their effects on
soil enzymology and microbial diversity are seldom reported. The present work reports on beneficial
effects of dung beetles on soil enzyme activity, bacterial diversity and growth and antioxidant content
of Chinese cabbage (Brassica rapa ssp. pekinensis). The soil amended with cow dung only (CD), or
with dung broken down by dung beetles (DB), was compared with untreated control (CO). Soil
bacterial diversity was determined through 16S rRNA gene sequencing, and enzymological tests were
used for soil enzyme activity. Dung beetles significantly increased acid phosphatase, urease,
cellulase, catalase and β-glucosidase activities in the soil compared with CD or control. Bacterial
alpha-diversity significantly increased in DB when compared with CD or control. Microbial carbon
(C) and nitrogen (N) were significantly higher in DB than in CD and in CD than control. The DB and
CD treatments also significantly enhanced plant growth and the content of antioxidants (total
flavonoids, polyphenols and vitamin C) and phytohormones indole-3-acetic acid (IAA), trans-zeatin
riboside (ZR) and gibberellic acid (GA3) compared with control, while a significant reduction was
noted in abscisic acid (ABA) in DB compared with CD or control plants. Correlation analysis
confirmed that the enzyme activities are pH dependent, as pH was significantly lowered by DB
compared with CD or control. Our results suggest that use of dung beetles in conventional practices
could be an effective strategy to improve soil nutrient availability and microbial activity and
antioxidant content of Chinese cabbage.",
publisher = "Springer",
journal = "Journal of Soil Science and Plant Nutrition",
title = "Dung Beetle Improves soil Bacterial Diversity and Enzyme Activity and Enhances Growth and  Antioxidant Content of Chinese Cabbage (Brassica rapa ssp. pekinensis)",
number = "4",
volume = "21",
doi = "10.1007/s42729-021-00614-w",
pages = "3387-3401"
}

Kaleri, A. R., Ma, J., Abro, S. A., Faqir, Y., Nabi, F., Hakeem, A., Ahmed, A., Ahmed, S., Jakhar, A. M., Shah, S. M., Tan, C., Qing, Y.,& Raspor, M.. (2021). Dung Beetle Improves soil Bacterial Diversity and Enzyme Activity and Enhances Growth and  Antioxidant Content of Chinese Cabbage (Brassica rapa ssp. pekinensis). in Journal of Soil Science and Plant Nutrition
Springer., 21(4), 3387-3401.
https://doi.org/10.1007/s42729-021-00614-w

Kaleri AR, Ma J, Abro SA, Faqir Y, Nabi F, Hakeem A, Ahmed A, Ahmed S, Jakhar AM, Shah SM, Tan C, Qing Y, Raspor M. Dung Beetle Improves soil Bacterial Diversity and Enzyme Activity and Enhances Growth and  Antioxidant Content of Chinese Cabbage (Brassica rapa ssp. pekinensis). in Journal of Soil Science and Plant Nutrition. 2021;21(4):3387-3401.
doi:10.1007/s42729-021-00614-w .

Kaleri, Abdul Rasheed, Ma, Jiahua, Abro, Saeed Akhtar, Faqir, Yahya, Nabi, Farhan, Hakeem, Abdul, Ahmed, Awais, Ahmed, Siraj, Jakhar, Ali Murad, Shah, Sayed Mustajab, Tan, Chengjia, Qing, Yuxin, Raspor, Martin, "Dung Beetle Improves soil Bacterial Diversity and Enzyme Activity and Enhances Growth and  Antioxidant Content of Chinese Cabbage (Brassica rapa ssp. pekinensis)" in Journal of Soil Science and Plant Nutrition, 21, no. 4 (2021):3387-3401,
https://doi.org/10.1007/s42729-021-00614-w . .

TY  - JOUR
AU  - Yang, Guotao
AU  - Nabi, Farhan
AU  - Sajid, Sumbal
AU  - Kaleri, Abdul Rasheed
AU  - Jakhar, Ali Murad
AU  - Cheng, Liang
AU  - Raspor, Martin
AU  - Muhammad, Noor
AU  - Ma, Jun
AU  - Hu, Yungao
PY  - 2021
UR  - https://link.springer.com/10.1007/s11033-021-06835-7
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4637
AB  - BACKGROUND Chemical fertilization helped modern agriculture in grain yield improvement to ensure food security. The response of chemical fertilization for higher hybrid rice production is highly dependent on optimal fertilization management in paddy fields. To assess such responses, in the current work we examine the yield, root growth, and expression of related genes responsible for stress metabolism of nitrogen (N) and phosphorus (P) in two hybrid-rice cultivars Deyou4727 (D47) and Yixiangyou2115 (Y21). METHODS AND RESULTS The experiment followed four nitrogen (N) (N0, N60, N120, and N180 kg/ha) and phosphorus (P) (P0, P60, P90, and P120 kg/ha) fertilizer levels. The grain yield in D47 was more sensitive to nitrogen application, while Y21 was more sensitive to phosphorus application, which resulted in comparatively higher biomass and yield. Our findings were corroborated by gene expression studies of glutamine synthetase OsGS1;1 and OsGS1;2 and phosphate starvation-related genes PHR1 and SPX, confirming sensitivity to N and P application. The number of roots was less sensitive to nitrogen application in D47 between N0 and N60, but the overall nutrient response difference was significantly higher due to the deep rooting system as compared to Y21. CONCLUSIONS The higher yield, high N and P use efficiency, and versatile root growth of D47 make it suitable to reduce unproductive usage of N and P from paddy fields, improving hybrid rice productivity, and environmental safety in the Sichuan basin area of China.
PB  - Springer Science and Business Media B.V.
T2  - Molecular Biology Reports
T1  - Response of root development and nutrient uptake of two chinese cultivars of hybrid rice to nitrogen and phosphorus fertilization in Sichuan Province, China.
IS  - 12
VL  - 48
DO  - 10.1007/s11033-021-06835-7
SP  - 8009
EP  - 821
ER  - 

@article{
author = "Yang, Guotao and Nabi, Farhan and Sajid, Sumbal and Kaleri, Abdul Rasheed and Jakhar, Ali Murad and Cheng, Liang and Raspor, Martin and Muhammad, Noor and Ma, Jun and Hu, Yungao",
year = "2021",
abstract = "BACKGROUND Chemical fertilization helped modern agriculture in grain yield improvement to ensure food security. The response of chemical fertilization for higher hybrid rice production is highly dependent on optimal fertilization management in paddy fields. To assess such responses, in the current work we examine the yield, root growth, and expression of related genes responsible for stress metabolism of nitrogen (N) and phosphorus (P) in two hybrid-rice cultivars Deyou4727 (D47) and Yixiangyou2115 (Y21). METHODS AND RESULTS The experiment followed four nitrogen (N) (N0, N60, N120, and N180 kg/ha) and phosphorus (P) (P0, P60, P90, and P120 kg/ha) fertilizer levels. The grain yield in D47 was more sensitive to nitrogen application, while Y21 was more sensitive to phosphorus application, which resulted in comparatively higher biomass and yield. Our findings were corroborated by gene expression studies of glutamine synthetase OsGS1;1 and OsGS1;2 and phosphate starvation-related genes PHR1 and SPX, confirming sensitivity to N and P application. The number of roots was less sensitive to nitrogen application in D47 between N0 and N60, but the overall nutrient response difference was significantly higher due to the deep rooting system as compared to Y21. CONCLUSIONS The higher yield, high N and P use efficiency, and versatile root growth of D47 make it suitable to reduce unproductive usage of N and P from paddy fields, improving hybrid rice productivity, and environmental safety in the Sichuan basin area of China.",
publisher = "Springer Science and Business Media B.V.",
journal = "Molecular Biology Reports",
title = "Response of root development and nutrient uptake of two chinese cultivars of hybrid rice to nitrogen and phosphorus fertilization in Sichuan Province, China.",
number = "12",
volume = "48",
doi = "10.1007/s11033-021-06835-7",
pages = "8009-821"
}

Yang, G., Nabi, F., Sajid, S., Kaleri, A. R., Jakhar, A. M., Cheng, L., Raspor, M., Muhammad, N., Ma, J.,& Hu, Y.. (2021). Response of root development and nutrient uptake of two chinese cultivars of hybrid rice to nitrogen and phosphorus fertilization in Sichuan Province, China.. in Molecular Biology Reports
Springer Science and Business Media B.V.., 48(12), 8009-821.
https://doi.org/10.1007/s11033-021-06835-7

Yang G, Nabi F, Sajid S, Kaleri AR, Jakhar AM, Cheng L, Raspor M, Muhammad N, Ma J, Hu Y. Response of root development and nutrient uptake of two chinese cultivars of hybrid rice to nitrogen and phosphorus fertilization in Sichuan Province, China.. in Molecular Biology Reports. 2021;48(12):8009-821.
doi:10.1007/s11033-021-06835-7 .

Yang, Guotao, Nabi, Farhan, Sajid, Sumbal, Kaleri, Abdul Rasheed, Jakhar, Ali Murad, Cheng, Liang, Raspor, Martin, Muhammad, Noor, Ma, Jun, Hu, Yungao, "Response of root development and nutrient uptake of two chinese cultivars of hybrid rice to nitrogen and phosphorus fertilization in Sichuan Province, China." in Molecular Biology Reports, 48, no. 12 (2021):8009-821,
https://doi.org/10.1007/s11033-021-06835-7 . .