TY  - JOUR
AU  - Pokimica, Nina
AU  - Ćosić, Tatjana
AU  - Uzelac, Branka
AU  - Ninković, Slavica
AU  - Raspor, Martin
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6631
AB  - Numerous biotechnological applications require a fast and efficient clonal propagation of whole plants under controlled laboratory conditions. For most plant species, the de novo regeneration of shoots from the cuttings of various plant organs can be obtained on nutrient media supplemented with plant hormones, auxin and cytokinin. While auxin is needed during the early stages of the process that include the establishment of pluripotent primordia and the subsequent acquisition of organogenic competence, cytokinin-supplemented media are required to induce these primordia to differentiate into developing shoots. The perception of cytokinin through the receptor ARABIDOPSIS HISTIDINE KINASE4 (AHK4) is crucial for the activation of the two main regulators of the establishment and maintenance of shoot apical meristems (SAMs): SHOOTMERISTEMLESS (STM) and the WUSCHEL-CLAVATA3 (WUS-CLV3) regulatory circuit. In this review, we summarize the current knowledge of the roles of the cytokinin signaling cascade in the perception and transduction of signals that are crucial for the de novo establishment of SAMs and lead to the desired biotechnological output—adventitious shoot multiplication. We highlight the functional differences between individual members of the multigene families involved in cytokinin signal transduction, and demonstrate how complex genetic regulation can be achieved through functional specialization of individual gene family members.
PB  - Basel: MDPI
T2  - Biomolecules
T1  - Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation
IS  - 3
VL  - 14
DO  - 10.3390/biom14030381
SP  - 381
ER  - 

@article{
author = "Pokimica, Nina and Ćosić, Tatjana and Uzelac, Branka and Ninković, Slavica and Raspor, Martin",
year = "2024",
abstract = "Numerous biotechnological applications require a fast and efficient clonal propagation of whole plants under controlled laboratory conditions. For most plant species, the de novo regeneration of shoots from the cuttings of various plant organs can be obtained on nutrient media supplemented with plant hormones, auxin and cytokinin. While auxin is needed during the early stages of the process that include the establishment of pluripotent primordia and the subsequent acquisition of organogenic competence, cytokinin-supplemented media are required to induce these primordia to differentiate into developing shoots. The perception of cytokinin through the receptor ARABIDOPSIS HISTIDINE KINASE4 (AHK4) is crucial for the activation of the two main regulators of the establishment and maintenance of shoot apical meristems (SAMs): SHOOTMERISTEMLESS (STM) and the WUSCHEL-CLAVATA3 (WUS-CLV3) regulatory circuit. In this review, we summarize the current knowledge of the roles of the cytokinin signaling cascade in the perception and transduction of signals that are crucial for the de novo establishment of SAMs and lead to the desired biotechnological output—adventitious shoot multiplication. We highlight the functional differences between individual members of the multigene families involved in cytokinin signal transduction, and demonstrate how complex genetic regulation can be achieved through functional specialization of individual gene family members.",
publisher = "Basel: MDPI",
journal = "Biomolecules",
title = "Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation",
number = "3",
volume = "14",
doi = "10.3390/biom14030381",
pages = "381"
}

Pokimica, N., Ćosić, T., Uzelac, B., Ninković, S.,& Raspor, M.. (2024). Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation. in Biomolecules
Basel: MDPI., 14(3), 381.
https://doi.org/10.3390/biom14030381

Pokimica N, Ćosić T, Uzelac B, Ninković S, Raspor M. Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation. in Biomolecules. 2024;14(3):381.
doi:10.3390/biom14030381 .

Pokimica, Nina, Ćosić, Tatjana, Uzelac, Branka, Ninković, Slavica, Raspor, Martin, "Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation" in Biomolecules, 14, no. 3 (2024):381,
https://doi.org/10.3390/biom14030381 . .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Anđela, Tomić
AU  - Uzelac, Branka
AU  - Raspor, Martin
AU  - Pokimica, Nina
AU  - Devrnja, Nina
AU  - Ninković, Slavica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6111
AB  - Deposition of carbohydrates in the lower part of the stem represents a unique feature of kohlrabi (Brassica
oleracea var. gongylodes), a member of Brassicaceae family grown for its high nutritional value. Over the
past decade our team intensely investigated in vitro development and regeneration of this significant crop,
determining, among other specific characteristics, an intricate relation between sucrose, serving as a carbon
source for in vitro growth, and cytokinins (CKs), utilized for the induction of indirect de novo shoot
organogenesis (DNSO). Even though the formation of kohlrabi stem tuber in vitro has not yet been
successfully attained, development of an efficient in vitro system for kohlrabi growth and DNSO opened the
possibility to study sugar metabolism in young kohlrabi plants, including the effects of elevated sucrose
application (6% and 9%) and CKs (trans-zeatin, transZ; thidiazuron, TDZ). Expression analysis of ten
selected genes involved in sugar partitioning in plants, indicated that both sucrose and CKs had similar
influence in the sense of downregulating these genes compared to control grown on plant growth
regulator-free medium with 3% sucrose. TDZ application had the highest effect on relative expression of all
three sucrose synthase genes (SUS1/3/6), particularly in three week-old plantlets (S1), the developmental
stage characterized by callus formation in the CK-treated kohlrabi. This trend was also observed for the
CWINV1, coding for β-fructofuranosidase and UGP1, coding for UTP--glucose-1-phosphate uridylyltransferase
1. On the other hand, high concentration of sucrose induced stronger downregulation of CINV1 (alkaline/
neutral invertase1), HXK1 (hexokinase1), GPI (glucose-6-phosphate isomerase) and SPS1 (sucrose-phosphate
synthase 1). TransZ led to small changes in gene expression, and even led to upregulation of SUS1 and
CINV1. Older plantlets (S2, four week-old, forming de novo shoots on CK treatment) were generally less
affected by applied treatments. In addition, histological sections of treated kohlrabi hypocotyls revealed
distinct changes at morpho-anatomical level. Our results, for the first time, depict effects of sucrose and CK
on activity of soluble sugars-related gene in young kohlrabi, suggesting an altered metabolism and transport
of soluble sugars, providing a starting point to the elucidation of stem tuber development and sugar
partitioning in kohlrabi.
PB  - Daejeon: International Association For Plant Biotechnology
C3  - Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
T1  - Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi
SP  - 345
EP  - 345
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6111
ER  - 

@conference{
author = "Ćosić, Tatjana and Anđela, Tomić and Uzelac, Branka and Raspor, Martin and Pokimica, Nina and Devrnja, Nina and Ninković, Slavica",
year = "2023",
abstract = "Deposition of carbohydrates in the lower part of the stem represents a unique feature of kohlrabi (Brassica
oleracea var. gongylodes), a member of Brassicaceae family grown for its high nutritional value. Over the
past decade our team intensely investigated in vitro development and regeneration of this significant crop,
determining, among other specific characteristics, an intricate relation between sucrose, serving as a carbon
source for in vitro growth, and cytokinins (CKs), utilized for the induction of indirect de novo shoot
organogenesis (DNSO). Even though the formation of kohlrabi stem tuber in vitro has not yet been
successfully attained, development of an efficient in vitro system for kohlrabi growth and DNSO opened the
possibility to study sugar metabolism in young kohlrabi plants, including the effects of elevated sucrose
application (6% and 9%) and CKs (trans-zeatin, transZ; thidiazuron, TDZ). Expression analysis of ten
selected genes involved in sugar partitioning in plants, indicated that both sucrose and CKs had similar
influence in the sense of downregulating these genes compared to control grown on plant growth
regulator-free medium with 3% sucrose. TDZ application had the highest effect on relative expression of all
three sucrose synthase genes (SUS1/3/6), particularly in three week-old plantlets (S1), the developmental
stage characterized by callus formation in the CK-treated kohlrabi. This trend was also observed for the
CWINV1, coding for β-fructofuranosidase and UGP1, coding for UTP--glucose-1-phosphate uridylyltransferase
1. On the other hand, high concentration of sucrose induced stronger downregulation of CINV1 (alkaline/
neutral invertase1), HXK1 (hexokinase1), GPI (glucose-6-phosphate isomerase) and SPS1 (sucrose-phosphate
synthase 1). TransZ led to small changes in gene expression, and even led to upregulation of SUS1 and
CINV1. Older plantlets (S2, four week-old, forming de novo shoots on CK treatment) were generally less
affected by applied treatments. In addition, histological sections of treated kohlrabi hypocotyls revealed
distinct changes at morpho-anatomical level. Our results, for the first time, depict effects of sucrose and CK
on activity of soluble sugars-related gene in young kohlrabi, suggesting an altered metabolism and transport
of soluble sugars, providing a starting point to the elucidation of stem tuber development and sugar
partitioning in kohlrabi.",
publisher = "Daejeon: International Association For Plant Biotechnology",
journal = "Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea",
title = "Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi",
pages = "345-345",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6111"
}

Ćosić, T., Anđela, T., Uzelac, B., Raspor, M., Pokimica, N., Devrnja, N.,& Ninković, S.. (2023). Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
Daejeon: International Association For Plant Biotechnology., 345-345.
https://hdl.handle.net/21.15107/rcub_ibiss_6111

Ćosić T, Anđela T, Uzelac B, Raspor M, Pokimica N, Devrnja N, Ninković S. Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea. 2023;:345-345.
https://hdl.handle.net/21.15107/rcub_ibiss_6111 .

Ćosić, Tatjana, Anđela, Tomić, Uzelac, Branka, Raspor, Martin, Pokimica, Nina, Devrnja, Nina, Ninković, Slavica, "Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi" in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea (2023):345-345,
https://hdl.handle.net/21.15107/rcub_ibiss_6111 .