TY  - JOUR
AU  - Pešić, Milica
AU  - Podolski-Renić, Ana
AU  - Stojković Burić, Sonja
AU  - Matovic, Branko
AU  - Zmejkoski, Danica
AU  - Kojic, Vesna
AU  - Bogdanovic, Gordana
AU  - Pavicevic, Aleksandra
AU  - Mojovic, Milos
AU  - Savic, Aleksandar
AU  - Milenkovic, Ivana
AU  - Kalauzi, Aleksandar
AU  - Radotic, Ksenija
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1957
AB  - Data on medical applications of cerium oxide nanoparticles CeO2 (CONP)
   are promising, yet information regarding their action in cells is
   incomplete and there are conflicting reports about in vitro toxicity.
   Herein, we have studied cytotoxic effect of CONP in several cancer and
   normal cell lines and their potential to change intracellular redox
   status. The IC50 was achieved only in two of eight tested cell lines,
   melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating
   room temperature method was applied to produce CONP with an average
   crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2-
   vacancies. The induction of cell death by CONP and the production of
   reactive oxygen species (ROS) were analyzed by flow-cytometry. Free
   radicals related antioxidant capacity of the cells was studied by the
   reduction of stable free radical TEMPONE using electron spin resonance
   spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell
   lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small
   cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while
   normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5)
   were insensitive. The most sensitive were 518A2 melanoma and HT-29
   colorectal adenocarcinoma cell lines, with the IC50 values being between
   100 and 200 mu M. Decreased rate of TEMPONE reduction and increased
   production of certain ROS species (peroxynitrite and hydrogen peroxide
   anion) indicates that free radical metabolism, thus redox status was
   changed, and antioxidant capacity damaged in the CONP treated 518A2 and
   HT-29 cells. In conclusion, changes in intracellular redox status
   induced by CONP are partly attributed to the prooxidant activity of the
   nanoparticles. Further, ROS induced cell damages might eventually lead
   to the cell death. However, low inhibitory potential of CONP in the
   other human cell lines tested indicates that CONP may be safe for human
   usage in industry and medicine. (C) 2015 Elsevier Ireland Ltd. All
   rights reserved.
T2  - Chemico-Biological Interactions
T1  - Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity
VL  - 232
DO  - 10.1016/j.cbi.2015.03.013
SP  - 85
EP  - 93
ER  - 

@article{
author = "Pešić, Milica and Podolski-Renić, Ana and Stojković Burić, Sonja and Matovic, Branko and Zmejkoski, Danica and Kojic, Vesna and Bogdanovic, Gordana and Pavicevic, Aleksandra and Mojovic, Milos and Savic, Aleksandar and Milenkovic, Ivana and Kalauzi, Aleksandar and Radotic, Ksenija",
year = "2015",
abstract = "Data on medical applications of cerium oxide nanoparticles CeO2 (CONP)
   are promising, yet information regarding their action in cells is
   incomplete and there are conflicting reports about in vitro toxicity.
   Herein, we have studied cytotoxic effect of CONP in several cancer and
   normal cell lines and their potential to change intracellular redox
   status. The IC50 was achieved only in two of eight tested cell lines,
   melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating
   room temperature method was applied to produce CONP with an average
   crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2-
   vacancies. The induction of cell death by CONP and the production of
   reactive oxygen species (ROS) were analyzed by flow-cytometry. Free
   radicals related antioxidant capacity of the cells was studied by the
   reduction of stable free radical TEMPONE using electron spin resonance
   spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell
   lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small
   cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while
   normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5)
   were insensitive. The most sensitive were 518A2 melanoma and HT-29
   colorectal adenocarcinoma cell lines, with the IC50 values being between
   100 and 200 mu M. Decreased rate of TEMPONE reduction and increased
   production of certain ROS species (peroxynitrite and hydrogen peroxide
   anion) indicates that free radical metabolism, thus redox status was
   changed, and antioxidant capacity damaged in the CONP treated 518A2 and
   HT-29 cells. In conclusion, changes in intracellular redox status
   induced by CONP are partly attributed to the prooxidant activity of the
   nanoparticles. Further, ROS induced cell damages might eventually lead
   to the cell death. However, low inhibitory potential of CONP in the
   other human cell lines tested indicates that CONP may be safe for human
   usage in industry and medicine. (C) 2015 Elsevier Ireland Ltd. All
   rights reserved.",
journal = "Chemico-Biological Interactions",
title = "Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity",
volume = "232",
doi = "10.1016/j.cbi.2015.03.013",
pages = "85-93"
}

Pešić, M., Podolski-Renić, A., Stojković Burić, S., Matovic, B., Zmejkoski, D., Kojic, V., Bogdanovic, G., Pavicevic, A., Mojovic, M., Savic, A., Milenkovic, I., Kalauzi, A.,& Radotic, K.. (2015). Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity. in Chemico-Biological Interactions, 232, 85-93.
https://doi.org/10.1016/j.cbi.2015.03.013

Pešić M, Podolski-Renić A, Stojković Burić S, Matovic B, Zmejkoski D, Kojic V, Bogdanovic G, Pavicevic A, Mojovic M, Savic A, Milenkovic I, Kalauzi A, Radotic K. Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity. in Chemico-Biological Interactions. 2015;232:85-93.
doi:10.1016/j.cbi.2015.03.013 .

Pešić, Milica, Podolski-Renić, Ana, Stojković Burić, Sonja, Matovic, Branko, Zmejkoski, Danica, Kojic, Vesna, Bogdanovic, Gordana, Pavicevic, Aleksandra, Mojovic, Milos, Savic, Aleksandar, Milenkovic, Ivana, Kalauzi, Aleksandar, Radotic, Ksenija, "Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity" in Chemico-Biological Interactions, 232 (2015):85-93,
https://doi.org/10.1016/j.cbi.2015.03.013 . .