TY  - JOUR
AU  - Sekulić, Slobodan
AU  - Stilinović, Nebojša
AU  - Baturan, Branislava
AU  - Krsman, Anita
AU  - Tešić, Igor
AU  - Vejnović, Aleksandra
AU  - Petrović, Đorđe
AU  - Nikolašević, Željka
AU  - Mijavec, Anna
AU  - Pešić, Vesna
AU  - Petković, Branka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6057
AB  - Background: Breech presentation (BP) results from at random filling of the intrauterine cavity, with an equal probability for a BP or cephalic presentation (CP). Each fetus in BP has its "pair" in CP randomly assumed CP. Direct comparison of BP and CP makes bias to less expressed differences between these two groups. It is therefore necessary to subtract the number of fetuses/newborns from the CP set that are identical to the number of fetuses/newborns in the BP set, with identical characteristics, and add this group to the BP set before comparing them to the rest of the CP fetuses/newborns in the matching process. Methods: The procedure encompasses nine variables in pregnancies with a congenitally malformed uterus (CMU) identified at the Department of Obstetrics (1985-2014): gestational age, birth mass, birth length, head circumference, shoulders circumference, umbilical length, placental weight, newborn mass/newborn length ratio, and newborn mass/placental mass ratio. Firstly, the probability of BP was determined and its relation to gestational age, physical characteristics, and previous presentations. Then direct comparison as well as case-control matching of the CP and BP were performed. Case-control matching was based on either a single specific variable (M1) or all combined variables (M2). Findings: 462 deliveries were identified with CMU. In 81 cases of multiparity, a fetal presentation was found to be an independent event regardless of the previous presentation, gestational age, and newborn physical characteristics. In four types of CMU with 337 deliveries (Bicornuate, Didelphys, Unicornuate, Arcuate), 9 variables with 36 instances of comparison were observed. M1 in 10 instances and M2 in 6 instances showed a statistically significant lower value of breech/random presentation compared with CP. CP have lower value in 2 instances in M1 and 1 in M2. Statistically significant differences were absent without the matching process. Interpretations: The study confirms the maximum probability for the BP is 50%. The case-control matching procedure shows that it is able to detect the difference between the breech/random presentation and CP, while the classic method of direct comparison was unable to detect any differences. The outcome of the breech/random presentation in CMU should be evaluated with the described case-control matching procedure.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Medicine
T1  - Corrected evaluation of the breech presentation outcome based on etiology of this presentation in congenitally malformed uterus
VL  - 10
DO  - 10.3389/fmed.2023.1160229
SP  - 1160229
ER  - 

@article{
author = "Sekulić, Slobodan and Stilinović, Nebojša and Baturan, Branislava and Krsman, Anita and Tešić, Igor and Vejnović, Aleksandra and Petrović, Đorđe and Nikolašević, Željka and Mijavec, Anna and Pešić, Vesna and Petković, Branka",
year = "2023",
abstract = "Background: Breech presentation (BP) results from at random filling of the intrauterine cavity, with an equal probability for a BP or cephalic presentation (CP). Each fetus in BP has its "pair" in CP randomly assumed CP. Direct comparison of BP and CP makes bias to less expressed differences between these two groups. It is therefore necessary to subtract the number of fetuses/newborns from the CP set that are identical to the number of fetuses/newborns in the BP set, with identical characteristics, and add this group to the BP set before comparing them to the rest of the CP fetuses/newborns in the matching process. Methods: The procedure encompasses nine variables in pregnancies with a congenitally malformed uterus (CMU) identified at the Department of Obstetrics (1985-2014): gestational age, birth mass, birth length, head circumference, shoulders circumference, umbilical length, placental weight, newborn mass/newborn length ratio, and newborn mass/placental mass ratio. Firstly, the probability of BP was determined and its relation to gestational age, physical characteristics, and previous presentations. Then direct comparison as well as case-control matching of the CP and BP were performed. Case-control matching was based on either a single specific variable (M1) or all combined variables (M2). Findings: 462 deliveries were identified with CMU. In 81 cases of multiparity, a fetal presentation was found to be an independent event regardless of the previous presentation, gestational age, and newborn physical characteristics. In four types of CMU with 337 deliveries (Bicornuate, Didelphys, Unicornuate, Arcuate), 9 variables with 36 instances of comparison were observed. M1 in 10 instances and M2 in 6 instances showed a statistically significant lower value of breech/random presentation compared with CP. CP have lower value in 2 instances in M1 and 1 in M2. Statistically significant differences were absent without the matching process. Interpretations: The study confirms the maximum probability for the BP is 50%. The case-control matching procedure shows that it is able to detect the difference between the breech/random presentation and CP, while the classic method of direct comparison was unable to detect any differences. The outcome of the breech/random presentation in CMU should be evaluated with the described case-control matching procedure.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Medicine",
title = "Corrected evaluation of the breech presentation outcome based on etiology of this presentation in congenitally malformed uterus",
volume = "10",
doi = "10.3389/fmed.2023.1160229",
pages = "1160229"
}

Sekulić, S., Stilinović, N., Baturan, B., Krsman, A., Tešić, I., Vejnović, A., Petrović, Đ., Nikolašević, Ž., Mijavec, A., Pešić, V.,& Petković, B.. (2023). Corrected evaluation of the breech presentation outcome based on etiology of this presentation in congenitally malformed uterus. in Frontiers in Medicine
Lausanne: Frontiers Media SA., 10, 1160229.
https://doi.org/10.3389/fmed.2023.1160229

Sekulić S, Stilinović N, Baturan B, Krsman A, Tešić I, Vejnović A, Petrović Đ, Nikolašević Ž, Mijavec A, Pešić V, Petković B. Corrected evaluation of the breech presentation outcome based on etiology of this presentation in congenitally malformed uterus. in Frontiers in Medicine. 2023;10:1160229.
doi:10.3389/fmed.2023.1160229 .

Sekulić, Slobodan, Stilinović, Nebojša, Baturan, Branislava, Krsman, Anita, Tešić, Igor, Vejnović, Aleksandra, Petrović, Đorđe, Nikolašević, Željka, Mijavec, Anna, Pešić, Vesna, Petković, Branka, "Corrected evaluation of the breech presentation outcome based on etiology of this presentation in congenitally malformed uterus" in Frontiers in Medicine, 10 (2023):1160229,
https://doi.org/10.3389/fmed.2023.1160229 . .

TY  - JOUR
AU  - Podgorac, Jelena
AU  - Sekulić, Slobodan
AU  - Petković, Branka
AU  - Stojadinović, Gordana
AU  - Martać, Ljiljana
AU  - Pešić, Vesna
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9557044
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5169
AB  - Clinical findings show that the use of valproic acid (VPA) during pregnancy increases the risk of birth defects and autism spectrum disorder in offspring. Although there is a consensus that monitoring of potential long-term outcomes of VPA exposure is needed, especially in undiagnosed individuals, preclinical studies addressing this issue are rare. The present study examined the effects of continuous intrauterine exposure to a wide dose range of VPA (50, 100, 200, and 400 mg/kg/day) on the physical and behavioral response in peripubertal mice as a rodent model of adolescence. Body weight and the hot plate test [on postnatal days (PND) 25 and 32], the elevated plus-maze test (on PND35), and the open field test (on PND40) served to examine physical growth, the supraspinal reflex response to a painful thermal stimulus and conditional learning, anxiety-like/risk-assessment behavior, as well as novelty-induced psychomotor activity, respectively. VPA exposure produced the following responses: (i) a negative effect on body weight, except for the dose of 100 mg/kg/day in both sexes; (ii) an increase in the percentage of animals that responded to the thermal stimulus above the defined cut-off time interval and the response latency in both sexes; (iii) dose-specific changes within sexes in behavior provoked by a novel anxiogenic environment, i.e., in females less anxiety-like/risk-assessment behavior in response to the lowest exposure dose, and in males more pronounced anxiety-like/risk-assessment behavior after exposure to the highest dose and 100 mg/kg/day; (iv) dose-specific changes within sexes in novelty-induced psychomotor activity, i.e., in females a decrease in stereotypy-like activity along with an increase in rearing, and in males a decrease in stereotypy-like activity only. These findings show that continuous intrauterine exposure to VPA produces maladaptive functioning in different behavioral domains in adolescence and that the consequences are delicate to assess as they are dose-related within sexes.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Behavioral Neuroscience
T1  - The influence of continuous prenatal exposure to valproic acid on physical, nociceptive, emotional and psychomotor responses during adolescence in mice: Dose-related effects within sexes
VL  - 16
DO  - 10.3389/fnbeh.2022.982811
SP  - 982811
ER  - 

@article{
author = "Podgorac, Jelena and Sekulić, Slobodan and Petković, Branka and Stojadinović, Gordana and Martać, Ljiljana and Pešić, Vesna",
year = "2022",
abstract = "Clinical findings show that the use of valproic acid (VPA) during pregnancy increases the risk of birth defects and autism spectrum disorder in offspring. Although there is a consensus that monitoring of potential long-term outcomes of VPA exposure is needed, especially in undiagnosed individuals, preclinical studies addressing this issue are rare. The present study examined the effects of continuous intrauterine exposure to a wide dose range of VPA (50, 100, 200, and 400 mg/kg/day) on the physical and behavioral response in peripubertal mice as a rodent model of adolescence. Body weight and the hot plate test [on postnatal days (PND) 25 and 32], the elevated plus-maze test (on PND35), and the open field test (on PND40) served to examine physical growth, the supraspinal reflex response to a painful thermal stimulus and conditional learning, anxiety-like/risk-assessment behavior, as well as novelty-induced psychomotor activity, respectively. VPA exposure produced the following responses: (i) a negative effect on body weight, except for the dose of 100 mg/kg/day in both sexes; (ii) an increase in the percentage of animals that responded to the thermal stimulus above the defined cut-off time interval and the response latency in both sexes; (iii) dose-specific changes within sexes in behavior provoked by a novel anxiogenic environment, i.e., in females less anxiety-like/risk-assessment behavior in response to the lowest exposure dose, and in males more pronounced anxiety-like/risk-assessment behavior after exposure to the highest dose and 100 mg/kg/day; (iv) dose-specific changes within sexes in novelty-induced psychomotor activity, i.e., in females a decrease in stereotypy-like activity along with an increase in rearing, and in males a decrease in stereotypy-like activity only. These findings show that continuous intrauterine exposure to VPA produces maladaptive functioning in different behavioral domains in adolescence and that the consequences are delicate to assess as they are dose-related within sexes.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Behavioral Neuroscience",
title = "The influence of continuous prenatal exposure to valproic acid on physical, nociceptive, emotional and psychomotor responses during adolescence in mice: Dose-related effects within sexes",
volume = "16",
doi = "10.3389/fnbeh.2022.982811",
pages = "982811"
}

Podgorac, J., Sekulić, S., Petković, B., Stojadinović, G., Martać, L.,& Pešić, V.. (2022). The influence of continuous prenatal exposure to valproic acid on physical, nociceptive, emotional and psychomotor responses during adolescence in mice: Dose-related effects within sexes. in Frontiers in Behavioral Neuroscience
Lausanne: Frontiers Media S.A.., 16, 982811.
https://doi.org/10.3389/fnbeh.2022.982811

Podgorac J, Sekulić S, Petković B, Stojadinović G, Martać L, Pešić V. The influence of continuous prenatal exposure to valproic acid on physical, nociceptive, emotional and psychomotor responses during adolescence in mice: Dose-related effects within sexes. in Frontiers in Behavioral Neuroscience. 2022;16:982811.
doi:10.3389/fnbeh.2022.982811 .

Podgorac, Jelena, Sekulić, Slobodan, Petković, Branka, Stojadinović, Gordana, Martać, Ljiljana, Pešić, Vesna, "The influence of continuous prenatal exposure to valproic acid on physical, nociceptive, emotional and psychomotor responses during adolescence in mice: Dose-related effects within sexes" in Frontiers in Behavioral Neuroscience, 16 (2022):982811,
https://doi.org/10.3389/fnbeh.2022.982811 . .

TY  - JOUR
AU  - Sekulić, Slobodan
AU  - Jovanović, Aleksandar
AU  - Živanović, Željko
AU  - Simić, Svetlana
AU  - Kesić, Srđan
AU  - Petković, Branka
AU  - Čapo, Ivan
AU  - van Loon, Jack J
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2214552422000153
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4958
AB  - The International Space Station (ISS) has the possibility to perform experiments regarding rodent reproduction in microgravity. The musculoskeletal system at birth in precocial rodent species more resembles the human than that of altricial rodent species. For precocial rodent species with body weight ≤ 500 g (limit of ISS) determined were: adult body mass, newborn body mass, head-body length, tail length, existing variants (wild, domesticated, laboratory), single/group housing, dry food consumption/24 h, water intake/24 h, basal metabolic rate mlO2/g/h, environmental temperature, sand baths, urine output ml/24 h, fecal output g/24 h, size of fecal droplet, hair length, life span, length of oestrus cycle, duration of pregnancy, building nest, litter size, stage of musculoskeletal maturity at birth, and the duration of weaning. Characteristics were obtained by searching SCOPUS as well as the World Wide Web with key words for each of the species in English, Latin and, local language name. These characteristics were compared in order to find most appropriate species. Twelve precocial rodent species were identified. There is not enough data for Common yellow-toothed cavy, and Eastern spiny mouse. Inappropriate species were: Gundis, Dassie rat are a more demanding species for appropriate tending, litter size is small; Octodon degus requires sand baths as well as a nest during the first two weeks after delivery; muscle maturity of Spiny mouse at birth (myotubular stage), does not correspond to the human (late histochemical stage); Chinchilla requires separately housing, daily sand baths, has upper limit of weight. Possibility of keeping Southern mountain cavy as pet animal, short estrus, large litter size, absence of the need for nest and sand baths, makes this species the most promising candidates for experiments on ISS. If an experiment is planned with exposing gravid animals before term of the birth, then they might be kept together in the existing Rodent Habitat (USA). If an experiment with birth in microgravity is planned on ISS, the existing habitats do not provide conditions for such an experiment. It is necessary to develop habitats for separate keeping of pregnant animals to enable the following: 1. undisturbed delivery 2. prevent the possibility of hurting the newborns 3. ensure adequate post-partum maternal care and nursing.
T2  - Life Sciences in Space Research
T1  - Which precocial rodent species is more suitable as the experimental model of microgravity influence on prenatal musculosketal development on international space station?
VL  - 33
DO  - 10.1016/j.lssr.2022.04.001
SP  - 48
EP  - 57
ER  - 

@article{
author = "Sekulić, Slobodan and Jovanović, Aleksandar and Živanović, Željko and Simić, Svetlana and Kesić, Srđan and Petković, Branka and Čapo, Ivan and van Loon, Jack J",
year = "2022",
abstract = "The International Space Station (ISS) has the possibility to perform experiments regarding rodent reproduction in microgravity. The musculoskeletal system at birth in precocial rodent species more resembles the human than that of altricial rodent species. For precocial rodent species with body weight ≤ 500 g (limit of ISS) determined were: adult body mass, newborn body mass, head-body length, tail length, existing variants (wild, domesticated, laboratory), single/group housing, dry food consumption/24 h, water intake/24 h, basal metabolic rate mlO2/g/h, environmental temperature, sand baths, urine output ml/24 h, fecal output g/24 h, size of fecal droplet, hair length, life span, length of oestrus cycle, duration of pregnancy, building nest, litter size, stage of musculoskeletal maturity at birth, and the duration of weaning. Characteristics were obtained by searching SCOPUS as well as the World Wide Web with key words for each of the species in English, Latin and, local language name. These characteristics were compared in order to find most appropriate species. Twelve precocial rodent species were identified. There is not enough data for Common yellow-toothed cavy, and Eastern spiny mouse. Inappropriate species were: Gundis, Dassie rat are a more demanding species for appropriate tending, litter size is small; Octodon degus requires sand baths as well as a nest during the first two weeks after delivery; muscle maturity of Spiny mouse at birth (myotubular stage), does not correspond to the human (late histochemical stage); Chinchilla requires separately housing, daily sand baths, has upper limit of weight. Possibility of keeping Southern mountain cavy as pet animal, short estrus, large litter size, absence of the need for nest and sand baths, makes this species the most promising candidates for experiments on ISS. If an experiment is planned with exposing gravid animals before term of the birth, then they might be kept together in the existing Rodent Habitat (USA). If an experiment with birth in microgravity is planned on ISS, the existing habitats do not provide conditions for such an experiment. It is necessary to develop habitats for separate keeping of pregnant animals to enable the following: 1. undisturbed delivery 2. prevent the possibility of hurting the newborns 3. ensure adequate post-partum maternal care and nursing.",
journal = "Life Sciences in Space Research",
title = "Which precocial rodent species is more suitable as the experimental model of microgravity influence on prenatal musculosketal development on international space station?",
volume = "33",
doi = "10.1016/j.lssr.2022.04.001",
pages = "48-57"
}

Sekulić, S., Jovanović, A., Živanović, Ž., Simić, S., Kesić, S., Petković, B., Čapo, I.,& van Loon, J. J.. (2022). Which precocial rodent species is more suitable as the experimental model of microgravity influence on prenatal musculosketal development on international space station?. in Life Sciences in Space Research, 33, 48-57.
https://doi.org/10.1016/j.lssr.2022.04.001

Sekulić S, Jovanović A, Živanović Ž, Simić S, Kesić S, Petković B, Čapo I, van Loon JJ. Which precocial rodent species is more suitable as the experimental model of microgravity influence on prenatal musculosketal development on international space station?. in Life Sciences in Space Research. 2022;33:48-57.
doi:10.1016/j.lssr.2022.04.001 .

Sekulić, Slobodan, Jovanović, Aleksandar, Živanović, Željko, Simić, Svetlana, Kesić, Srđan, Petković, Branka, Čapo, Ivan, van Loon, Jack J, "Which precocial rodent species is more suitable as the experimental model of microgravity influence on prenatal musculosketal development on international space station?" in Life Sciences in Space Research, 33 (2022):48-57,
https://doi.org/10.1016/j.lssr.2022.04.001 . .

TY  - JOUR
AU  - Stojadinović, Gordana
AU  - Martać, Ljiljana
AU  - Podgorac, Jelena
AU  - Spasić, Slađana Z.
AU  - Petković, Branka
AU  - Sekulić, Slobodan
AU  - Kesić, Srđan
PY  - 2020
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/5405
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4005
AB  - A detailed analysis of the anesthetic-induced modulation of intracerebellar electrical activity is an important step to understand the functional brain responses to anesthesia. We examined the electrical activity recorded from different cortical layers: molecular layer (ML), Purkinje cell layer (PCL), granular layer (GL) and the white matter (WM) in the vermian part of rat cerebellar lobule V during Nembutal anesthesia using spectral and fractal analysis. Spectral analysis revealed a difference in the mean relative power of delta (0.1-4.0 Hz) and theta (4.1-8.0 Hz) frequencies through the cerebellar layers. Compared to the ML, delta activity increased significantly in the GL, while theta activity decreased in the GL and the WM. Fractal analysis revealed that the mean value of Higuchi’s fractal dimension (HFD) increased, starting from the ML to the WM. Theta activity exhibited a negative correlation with the HFD value in the ML. In contrast, the gamma activity showed a positive correlation with the HFD value in the ML and the GL. The combined use of spectral and fractal analyses revealed that Nembutal displays different effects on rat cerebellar electrical activity, which largely depends on the neurochemical and electrophysiological organization of the cerebellar layers.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis
IS  - 3
VL  - 72
DO  - 10.2298/ABS200524036S
SP  - 425
EP  - 432
ER  - 

@article{
author = "Stojadinović, Gordana and Martać, Ljiljana and Podgorac, Jelena and Spasić, Slađana Z. and Petković, Branka and Sekulić, Slobodan and Kesić, Srđan",
year = "2020",
abstract = "A detailed analysis of the anesthetic-induced modulation of intracerebellar electrical activity is an important step to understand the functional brain responses to anesthesia. We examined the electrical activity recorded from different cortical layers: molecular layer (ML), Purkinje cell layer (PCL), granular layer (GL) and the white matter (WM) in the vermian part of rat cerebellar lobule V during Nembutal anesthesia using spectral and fractal analysis. Spectral analysis revealed a difference in the mean relative power of delta (0.1-4.0 Hz) and theta (4.1-8.0 Hz) frequencies through the cerebellar layers. Compared to the ML, delta activity increased significantly in the GL, while theta activity decreased in the GL and the WM. Fractal analysis revealed that the mean value of Higuchi’s fractal dimension (HFD) increased, starting from the ML to the WM. Theta activity exhibited a negative correlation with the HFD value in the ML. In contrast, the gamma activity showed a positive correlation with the HFD value in the ML and the GL. The combined use of spectral and fractal analyses revealed that Nembutal displays different effects on rat cerebellar electrical activity, which largely depends on the neurochemical and electrophysiological organization of the cerebellar layers.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis",
number = "3",
volume = "72",
doi = "10.2298/ABS200524036S",
pages = "425-432"
}

Stojadinović, G., Martać, L., Podgorac, J., Spasić, S. Z., Petković, B., Sekulić, S.,& Kesić, S.. (2020). The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis. in Archives of Biological Sciences
Serbian Biological Society., 72(3), 425-432.
https://doi.org/10.2298/ABS200524036S

Stojadinović G, Martać L, Podgorac J, Spasić SZ, Petković B, Sekulić S, Kesić S. The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis. in Archives of Biological Sciences. 2020;72(3):425-432.
doi:10.2298/ABS200524036S .

Stojadinović, Gordana, Martać, Ljiljana, Podgorac, Jelena, Spasić, Slađana Z., Petković, Branka, Sekulić, Slobodan, Kesić, Srđan, "The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis" in Archives of Biological Sciences, 72, no. 3 (2020):425-432,
https://doi.org/10.2298/ABS200524036S . .

TY  - JOUR
AU  - Čapo, Ivan
AU  - Milenković, Ivan
AU  - Čapo, Nataša
AU  - Stilinović, Nebojša
AU  - Vukmirović, Saša
AU  - Teofilovic, Branislava
AU  - Petković, Branka
AU  - Sekulić, Slobodan
PY  - 2020
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46642000041C
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4102
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/6035
AB  - The most sensitive period in brain development is during prenatal life. The use of antibiotics in pregnancy is still controversial. Recent studies revealed the high neurotoxic potential of the antibiotic and antiprotozoal medication, metronidazole. However, there are insufficient data from animal studies about prenatal treatment effects. We investigated the effect of prenatal treatment with metronidazole on cerebellar development in guinea pigs. Treatment with metronidazole was performed from the 42nd to the 49th day of gestation. On the 50th day of pregnancy, all dams were killed, and the cerebella of the fetuses were analyzed. Gross cerebellar changes characterized by malposition of the folia with partial atrophy were found in 12 of 19 fetuses in the experimental group, but in none of 20 control fetuses that received saline. The most affected were folia VII with depletion of the areal fraction of the external granular layer, molecular layer and the internal granular layer. Purkinje cells displayed cell distortion with loss of normal dendritic polarity. The investigation revealed cell depletion, with a disturbance of the cytoarchitectonic of the cerebellar cortex and folia alteration.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Prenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetuses
IS  - 4
VL  - 72
DO  - 10.2298/ABS200619041C
SP  - 473
EP  - 482
ER  - 

@article{
author = "Čapo, Ivan and Milenković, Ivan and Čapo, Nataša and Stilinović, Nebojša and Vukmirović, Saša and Teofilovic, Branislava and Petković, Branka and Sekulić, Slobodan",
year = "2020",
abstract = "The most sensitive period in brain development is during prenatal life. The use of antibiotics in pregnancy is still controversial. Recent studies revealed the high neurotoxic potential of the antibiotic and antiprotozoal medication, metronidazole. However, there are insufficient data from animal studies about prenatal treatment effects. We investigated the effect of prenatal treatment with metronidazole on cerebellar development in guinea pigs. Treatment with metronidazole was performed from the 42nd to the 49th day of gestation. On the 50th day of pregnancy, all dams were killed, and the cerebella of the fetuses were analyzed. Gross cerebellar changes characterized by malposition of the folia with partial atrophy were found in 12 of 19 fetuses in the experimental group, but in none of 20 control fetuses that received saline. The most affected were folia VII with depletion of the areal fraction of the external granular layer, molecular layer and the internal granular layer. Purkinje cells displayed cell distortion with loss of normal dendritic polarity. The investigation revealed cell depletion, with a disturbance of the cytoarchitectonic of the cerebellar cortex and folia alteration.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Prenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetuses",
number = "4",
volume = "72",
doi = "10.2298/ABS200619041C",
pages = "473-482"
}

Čapo, I., Milenković, I., Čapo, N., Stilinović, N., Vukmirović, S., Teofilovic, B., Petković, B.,& Sekulić, S.. (2020). Prenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetuses. in Archives of Biological Sciences
Serbian Biological Society., 72(4), 473-482.
https://doi.org/10.2298/ABS200619041C

Čapo I, Milenković I, Čapo N, Stilinović N, Vukmirović S, Teofilovic B, Petković B, Sekulić S. Prenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetuses. in Archives of Biological Sciences. 2020;72(4):473-482.
doi:10.2298/ABS200619041C .

Čapo, Ivan, Milenković, Ivan, Čapo, Nataša, Stilinović, Nebojša, Vukmirović, Saša, Teofilovic, Branislava, Petković, Branka, Sekulić, Slobodan, "Prenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetuses" in Archives of Biological Sciences, 72, no. 4 (2020):473-482,
https://doi.org/10.2298/ABS200619041C . .

TY  - JOUR
AU  - Podgorac, Jelena
AU  - Pešić, Vesna
AU  - Pavković, Željko
AU  - Martać, Ljiljana
AU  - Kanazir, Selma
AU  - Filipović, Ljupka
AU  - Sekulić, Slobodan
PY  - 2016
UR  - http://www.journals.elsevier.com/behavioural-brain-research/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5456
AB  - Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.
PB  - Amsterdam : Elsevier
T2  - Behavioural Brain Research
T1  - Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development
VL  - 311
DO  - 10.1016/j.bbr.2016.05.023
SP  - 99
EP  - 109
ER  - 

@article{
author = "Podgorac, Jelena and Pešić, Vesna and Pavković, Željko and Martać, Ljiljana and Kanazir, Selma and Filipović, Ljupka and Sekulić, Slobodan",
year = "2016",
abstract = "Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.",
publisher = "Amsterdam : Elsevier",
journal = "Behavioural Brain Research",
title = "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development",
volume = "311",
doi = "10.1016/j.bbr.2016.05.023",
pages = "99-109"
}

Podgorac, J., Pešić, V., Pavković, Ž., Martać, L., Kanazir, S., Filipović, L.,& Sekulić, S.. (2016). Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research
Amsterdam : Elsevier., 311, 99-109.
https://doi.org/10.1016/j.bbr.2016.05.023

Podgorac J, Pešić V, Pavković Ž, Martać L, Kanazir S, Filipović L, Sekulić S. Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research. 2016;311:99-109.
doi:10.1016/j.bbr.2016.05.023 .

Podgorac, Jelena, Pešić, Vesna, Pavković, Željko, Martać, Ljiljana, Kanazir, Selma, Filipović, Ljupka, Sekulić, Slobodan, "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development" in Behavioural Brain Research, 311 (2016):99-109,
https://doi.org/10.1016/j.bbr.2016.05.023 . .

TY  - JOUR
AU  - Podgorac, Jelena
AU  - Pešić, Vesna
AU  - Pavković, Željko
AU  - Martać, Ljiljana
AU  - Kanazir, Selma
AU  - Filipović, Ljupka
AU  - Sekulić, Slobodan
PY  - 2016
UR  - http://www.journals.elsevier.com/behavioural-brain-research/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5455
AB  - Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.
PB  - Amsterdam : Elsevier
T2  - Behavioural Brain Research
T1  - Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development
VL  - 311
DO  - 10.1016/j.bbr.2016.05.023
SP  - 99
EP  - 109
ER  - 

@article{
author = "Podgorac, Jelena and Pešić, Vesna and Pavković, Željko and Martać, Ljiljana and Kanazir, Selma and Filipović, Ljupka and Sekulić, Slobodan",
year = "2016",
abstract = "Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.",
publisher = "Amsterdam : Elsevier",
journal = "Behavioural Brain Research",
title = "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development",
volume = "311",
doi = "10.1016/j.bbr.2016.05.023",
pages = "99-109"
}

Podgorac, J., Pešić, V., Pavković, Ž., Martać, L., Kanazir, S., Filipović, L.,& Sekulić, S.. (2016). Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research
Amsterdam : Elsevier., 311, 99-109.
https://doi.org/10.1016/j.bbr.2016.05.023

Podgorac J, Pešić V, Pavković Ž, Martać L, Kanazir S, Filipović L, Sekulić S. Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research. 2016;311:99-109.
doi:10.1016/j.bbr.2016.05.023 .

Podgorac, Jelena, Pešić, Vesna, Pavković, Željko, Martać, Ljiljana, Kanazir, Selma, Filipović, Ljupka, Sekulić, Slobodan, "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development" in Behavioural Brain Research, 311 (2016):99-109,
https://doi.org/10.1016/j.bbr.2016.05.023 . .

TY  - JOUR
AU  - Martać, Ljiljana
AU  - Podgorac, Jelena
AU  - Petković, Branka
AU  - Sekulić, Slobodan
AU  - Čapo, Ivan
PY  - 2015
UR  - http://arpgweb.com/?ic=journal&journal=16&info=aims
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3777
AB  - This paper presents an overview of rat animal model of Alzheimer’s disease. Model is based on the
toxicity, oxidative stress and neurodegeneration. The model is obtained by treatment of rats with intraperitoneal aluminum. Electrocortical group neuron activity describes changes in neurotransmission caused by different factors. Such changes could be qualitatively described by spectral and fractal analysis of electrocortical activity as a variation of the relative spectral power. Both animals and patients with Alzheimers demention have increased relative spectral power in delta range. By fractal analysis we described changes in electrocortical activity of aluminum intoxication animals compare to physiological control. We used change in delta range to calculate fractal dimension. Also we used fractal dimension to compare treated animals with control ones to quantitatively describe the degree of pathophysiological state. All changes are correlated with an increase in the relative spectral power in the delta range and can be quantitatively described by fractal dimension. Results are presented as the effect and can also be applied to the human model of neurotoxicity and neurodegeneration like Alzheimer’s dementia. The model itself may be used for diagnostic and prognostic purposes since it describes the parameters underlying Alzheimer’s disease. However in definition of disease should be consider a state of dementia and menthal activity.
PB  - Academic Research Publishing Group
T2  - Journal of Biotechnology Research
T1  - Spectral and Fractal Analysis of ECoG in Animal Model of Aluminium Intoxication
IS  - 5
VL  - 1
SP  - 21
EP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3777
ER  - 

@article{
author = "Martać, Ljiljana and Podgorac, Jelena and Petković, Branka and Sekulić, Slobodan and Čapo, Ivan",
year = "2015",
abstract = "This paper presents an overview of rat animal model of Alzheimer’s disease. Model is based on the
toxicity, oxidative stress and neurodegeneration. The model is obtained by treatment of rats with intraperitoneal aluminum. Electrocortical group neuron activity describes changes in neurotransmission caused by different factors. Such changes could be qualitatively described by spectral and fractal analysis of electrocortical activity as a variation of the relative spectral power. Both animals and patients with Alzheimers demention have increased relative spectral power in delta range. By fractal analysis we described changes in electrocortical activity of aluminum intoxication animals compare to physiological control. We used change in delta range to calculate fractal dimension. Also we used fractal dimension to compare treated animals with control ones to quantitatively describe the degree of pathophysiological state. All changes are correlated with an increase in the relative spectral power in the delta range and can be quantitatively described by fractal dimension. Results are presented as the effect and can also be applied to the human model of neurotoxicity and neurodegeneration like Alzheimer’s dementia. The model itself may be used for diagnostic and prognostic purposes since it describes the parameters underlying Alzheimer’s disease. However in definition of disease should be consider a state of dementia and menthal activity.",
publisher = "Academic Research Publishing Group",
journal = "Journal of Biotechnology Research",
title = "Spectral and Fractal Analysis of ECoG in Animal Model of Aluminium Intoxication",
number = "5",
volume = "1",
pages = "21-25",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3777"
}

Martać, L., Podgorac, J., Petković, B., Sekulić, S.,& Čapo, I.. (2015). Spectral and Fractal Analysis of ECoG in Animal Model of Aluminium Intoxication. in Journal of Biotechnology Research
Academic Research Publishing Group., 1(5), 21-25.
https://hdl.handle.net/21.15107/rcub_ibiss_3777

Martać L, Podgorac J, Petković B, Sekulić S, Čapo I. Spectral and Fractal Analysis of ECoG in Animal Model of Aluminium Intoxication. in Journal of Biotechnology Research. 2015;1(5):21-25.
https://hdl.handle.net/21.15107/rcub_ibiss_3777 .

Martać, Ljiljana, Podgorac, Jelena, Petković, Branka, Sekulić, Slobodan, Čapo, Ivan, "Spectral and Fractal Analysis of ECoG in Animal Model of Aluminium Intoxication" in Journal of Biotechnology Research, 1, no. 5 (2015):21-25,
https://hdl.handle.net/21.15107/rcub_ibiss_3777 .