TY  - JOUR
AU  - Adžić Bukvić, Marija
AU  - Laketa, Danijela
AU  - Dragić, Milorad
AU  - Lavrnja, Irena
AU  - Nedeljković, Nadežda
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6368
AB  - Ecto-50-nucleotidase/CD73 (eN/CD73) is a membrane-bound enzyme involved in
extracellular production of adenosine and a cell adhesion molecule involved in cell–
cell interactions. In neuroinflammatory conditions such as experimental autoimmune
encephalomyelitis (EAE), reactive astrocytes occupying active demyelination areas
significantly upregulate eN/CD73 and express additional eN/CD73 variants. The present
study investigated whether the different eN/CD73 variants represent distinct
glycoforms and the functional consequences of their expression in neuroinflammatory
states. The study was performed in animals at different stages of EAE and in primary
astrocyte cultures treated with a range of inflammatory cytokines. Upregulation
at the mRNA, protein, and functional levels, as well as the appearance of multiple
eN/CD73 glycovariants were detected in the inflamed spinal cord tissue. At the peak
of the disease, eN/CD73 exhibited higher AMP turnover and lower enzymesubstrate
affinity than the control group, which was attributed to altered glycosylation
under neuroinflammatory conditions. A subsequent in vitro study showed that
primary astrocytes upregulated eN/CD73 and expressed the multiple glycovariants
upon stimulation with TNFα, IL-1β, IL-6, and ATP, with the effect occurring at least in
part via induction of JAK/STAT3 signaling. Experimental removal of glycan moieties
from membrane glycoproteins by PNGaseF decreased eN/CD73 activity but had no
effect on the enzyme's involvement in astrocyte migration. Our results suggest that
neuroinflammatory states are associated with the appearance of functionally distinct
eN/CD73 glycovariants, which may play a role in the development of the reactive
astrocyte phenotype.
PB  - Hoboken: Wiley
T2  - Glia
T1  - Expression of functionally distinct ecto-5'-nucleotidase/CD73 glycovariants in reactive astrocytes in experimental autoimmune encephalomyelitis and neuroinflammatory conditions in vitro
IS  - 1
VL  - 72
DO  - 10.1002/glia.24459
SP  - 19
EP  - 33
ER  - 

@article{
author = "Adžić Bukvić, Marija and Laketa, Danijela and Dragić, Milorad and Lavrnja, Irena and Nedeljković, Nadežda",
year = "2024",
abstract = "Ecto-50-nucleotidase/CD73 (eN/CD73) is a membrane-bound enzyme involved in
extracellular production of adenosine and a cell adhesion molecule involved in cell–
cell interactions. In neuroinflammatory conditions such as experimental autoimmune
encephalomyelitis (EAE), reactive astrocytes occupying active demyelination areas
significantly upregulate eN/CD73 and express additional eN/CD73 variants. The present
study investigated whether the different eN/CD73 variants represent distinct
glycoforms and the functional consequences of their expression in neuroinflammatory
states. The study was performed in animals at different stages of EAE and in primary
astrocyte cultures treated with a range of inflammatory cytokines. Upregulation
at the mRNA, protein, and functional levels, as well as the appearance of multiple
eN/CD73 glycovariants were detected in the inflamed spinal cord tissue. At the peak
of the disease, eN/CD73 exhibited higher AMP turnover and lower enzymesubstrate
affinity than the control group, which was attributed to altered glycosylation
under neuroinflammatory conditions. A subsequent in vitro study showed that
primary astrocytes upregulated eN/CD73 and expressed the multiple glycovariants
upon stimulation with TNFα, IL-1β, IL-6, and ATP, with the effect occurring at least in
part via induction of JAK/STAT3 signaling. Experimental removal of glycan moieties
from membrane glycoproteins by PNGaseF decreased eN/CD73 activity but had no
effect on the enzyme's involvement in astrocyte migration. Our results suggest that
neuroinflammatory states are associated with the appearance of functionally distinct
eN/CD73 glycovariants, which may play a role in the development of the reactive
astrocyte phenotype.",
publisher = "Hoboken: Wiley",
journal = "Glia",
title = "Expression of functionally distinct ecto-5'-nucleotidase/CD73 glycovariants in reactive astrocytes in experimental autoimmune encephalomyelitis and neuroinflammatory conditions in vitro",
number = "1",
volume = "72",
doi = "10.1002/glia.24459",
pages = "19-33"
}

Adžić Bukvić, M., Laketa, D., Dragić, M., Lavrnja, I.,& Nedeljković, N.. (2024). Expression of functionally distinct ecto-5'-nucleotidase/CD73 glycovariants in reactive astrocytes in experimental autoimmune encephalomyelitis and neuroinflammatory conditions in vitro. in Glia
Hoboken: Wiley., 72(1), 19-33.
https://doi.org/10.1002/glia.24459

Adžić Bukvić M, Laketa D, Dragić M, Lavrnja I, Nedeljković N. Expression of functionally distinct ecto-5'-nucleotidase/CD73 glycovariants in reactive astrocytes in experimental autoimmune encephalomyelitis and neuroinflammatory conditions in vitro. in Glia. 2024;72(1):19-33.
doi:10.1002/glia.24459 .

Adžić Bukvić, Marija, Laketa, Danijela, Dragić, Milorad, Lavrnja, Irena, Nedeljković, Nadežda, "Expression of functionally distinct ecto-5'-nucleotidase/CD73 glycovariants in reactive astrocytes in experimental autoimmune encephalomyelitis and neuroinflammatory conditions in vitro" in Glia, 72, no. 1 (2024):19-33,
https://doi.org/10.1002/glia.24459 . .