TY  - JOUR
AU  - Matić Petrović, Sanja
AU  - Nikolić, Nađa
AU  - Toljić, Boško
AU  - Arambašić Jovanović, Jelena
AU  - Miličić, Biljana
AU  - Miličić, Tanja
AU  - Jotić, Aleksandra
AU  - Vidaković, Melita
AU  - Milašin, Jelena
AU  - Pucar, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3945
AB  - Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between −308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed. Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA). Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037−0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35−7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954−0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D. Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.
PB  - Elsevier Ltd
T2  - Archives of Oral Biology
T1  - The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population
VL  - 120
DO  - 10.1016/j.archoralbio.2020.104929
SP  - 104929
ER  - 

@article{
author = "Matić Petrović, Sanja and Nikolić, Nađa and Toljić, Boško and Arambašić Jovanović, Jelena and Miličić, Biljana and Miličić, Tanja and Jotić, Aleksandra and Vidaković, Melita and Milašin, Jelena and Pucar, Ana",
year = "2020",
abstract = "Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between −308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed. Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA). Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037−0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35−7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954−0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D. Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.",
publisher = "Elsevier Ltd",
journal = "Archives of Oral Biology",
title = "The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population",
volume = "120",
doi = "10.1016/j.archoralbio.2020.104929",
pages = "104929"
}

Matić Petrović, S., Nikolić, N., Toljić, B., Arambašić Jovanović, J., Miličić, B., Miličić, T., Jotić, A., Vidaković, M., Milašin, J.,& Pucar, A.. (2020). The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. in Archives of Oral Biology
Elsevier Ltd., 120, 104929.
https://doi.org/10.1016/j.archoralbio.2020.104929

Matić Petrović S, Nikolić N, Toljić B, Arambašić Jovanović J, Miličić B, Miličić T, Jotić A, Vidaković M, Milašin J, Pucar A. The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. in Archives of Oral Biology. 2020;120:104929.
doi:10.1016/j.archoralbio.2020.104929 .

Matić Petrović, Sanja, Nikolić, Nađa, Toljić, Boško, Arambašić Jovanović, Jelena, Miličić, Biljana, Miličić, Tanja, Jotić, Aleksandra, Vidaković, Melita, Milašin, Jelena, Pucar, Ana, "The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population" in Archives of Oral Biology, 120 (2020):104929,
https://doi.org/10.1016/j.archoralbio.2020.104929 . .

TY  - CONF
AU  - Anđus, Stefan
AU  - Đuknić, Jelena
AU  - Tubić, Bojana
AU  - Nikolić, Nađa
AU  - Marjanović, Ana
AU  - Branković, Marija
AU  - Nikolić, Vera
AU  - Paunović, Momir
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4269
AB  - Sponges in Serbian rivers and lakes have not been extensively studied. Hence, the aim of this work was to undertake an investigation of the distribution and phylogenetics of sponge species in Serbian waterbodies. A total of 83 localities on 17 rivers and 10 lakes have been investigated. Sponges (62 specimens) were found at 22 localities only. Sponge determination was done using a combination of morphological and genetic studies. Light microscopy and scanning electron microscopy were applied for spicule analysis while the D3 domain of 28S DNA was amplified and sequenced for genetic determination. The following five sponge species were identified: E. fluviatilis, S. lacustris, E. muelleri, T. horrida and E. fragilis. The sequence of the Serbian E. fragilis differed in two base pairs compared to Estonian E. fragilis (sequence obtained from the database of the National Center for Biotechnology Information, U.S. National Library of Medicine – NCBI), the previously deposited sequence on NCBI from Estonian E. fragilis. The sequence of T. horrida from our study is the first partial 28S sequence deposited for this species in the NCBI. A phylogenetic tree based on the 340bp sequences was also generated. It showed distinctive clades and was concordant with the results of the morphological analysis.
PB  - Belgrade: Serbian Genetic Society
C3  - 6th Congress of the Serbian genetic society; 2019 Oct 13-17; Vrnjačka Banja, Serbia
T1  - Morphological and genetic analysis of freshwater sponges in Serbia
SP  - 174
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4269
ER  - 

@conference{
editor = "Vasiljević, Branka, Patenković, Aleksandra, Nikolić, Nađa",
author = "Anđus, Stefan and Đuknić, Jelena and Tubić, Bojana and Nikolić, Nađa and Marjanović, Ana and Branković, Marija and Nikolić, Vera and Paunović, Momir",
year = "2019",
abstract = "Sponges in Serbian rivers and lakes have not been extensively studied. Hence, the aim of this work was to undertake an investigation of the distribution and phylogenetics of sponge species in Serbian waterbodies. A total of 83 localities on 17 rivers and 10 lakes have been investigated. Sponges (62 specimens) were found at 22 localities only. Sponge determination was done using a combination of morphological and genetic studies. Light microscopy and scanning electron microscopy were applied for spicule analysis while the D3 domain of 28S DNA was amplified and sequenced for genetic determination. The following five sponge species were identified: E. fluviatilis, S. lacustris, E. muelleri, T. horrida and E. fragilis. The sequence of the Serbian E. fragilis differed in two base pairs compared to Estonian E. fragilis (sequence obtained from the database of the National Center for Biotechnology Information, U.S. National Library of Medicine – NCBI), the previously deposited sequence on NCBI from Estonian E. fragilis. The sequence of T. horrida from our study is the first partial 28S sequence deposited for this species in the NCBI. A phylogenetic tree based on the 340bp sequences was also generated. It showed distinctive clades and was concordant with the results of the morphological analysis.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "6th Congress of the Serbian genetic society; 2019 Oct 13-17; Vrnjačka Banja, Serbia",
title = "Morphological and genetic analysis of freshwater sponges in Serbia",
pages = "174",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4269"
}

Vasiljević, B., Patenković, A., Nikolić, N., Anđus, S., Đuknić, J., Tubić, B., Nikolić, N., Marjanović, A., Branković, M., Nikolić, V.,& Paunović, M.. (2019). Morphological and genetic analysis of freshwater sponges in Serbia. in 6th Congress of the Serbian genetic society; 2019 Oct 13-17; Vrnjačka Banja, Serbia
Belgrade: Serbian Genetic Society., 174.
https://hdl.handle.net/21.15107/rcub_ibiss_4269

Vasiljević B, Patenković A, Nikolić N, Anđus S, Đuknić J, Tubić B, Nikolić N, Marjanović A, Branković M, Nikolić V, Paunović M. Morphological and genetic analysis of freshwater sponges in Serbia. in 6th Congress of the Serbian genetic society; 2019 Oct 13-17; Vrnjačka Banja, Serbia. 2019;:174.
https://hdl.handle.net/21.15107/rcub_ibiss_4269 .

Vasiljević, Branka, Patenković, Aleksandra, Nikolić, Nađa, Anđus, Stefan, Đuknić, Jelena, Tubić, Bojana, Nikolić, Nađa, Marjanović, Ana, Branković, Marija, Nikolić, Vera, Paunović, Momir, "Morphological and genetic analysis of freshwater sponges in Serbia" in 6th Congress of the Serbian genetic society; 2019 Oct 13-17; Vrnjačka Banja, Serbia (2019):174,
https://hdl.handle.net/21.15107/rcub_ibiss_4269 .

TY  - JOUR
AU  - Anđus, Stefan
AU  - Nikolić, Nađa
AU  - Marjanović, Ana
AU  - Branković, Marija
AU  - Lazović, Vladimir
AU  - Tubić, Bojana
AU  - Čanak Atlagić, Jelena
AU  - Nikolić, Vera
AU  - Paunović, Momir
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0075951118302299?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3334
AB  - Trochospongilla horrida Weltner, 1893 (Spongillidae, Spongillida) has a relatively wide, though discontinuous, distribution in the Northern Hemisphere. The aim of this paper was to report the first finding of T. horrida in Serbia. During 2017, research was carried out on ten tributaries of the Sava and the Danube. T. horrida was identified at three localities in Tisa, Juzna Morava and Zapadna Morava rivers, out of 51 inspected sites. For the first time ribosomal 28S DNA was used for T. horrida identification.
T2  - Limnologica
T1  - First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia – A morphological and genetic study
VL  - 76
DO  - 10.1016/J.LIMNO.2019.03.004
SP  - 48
EP  - 51
ER  - 

@article{
author = "Anđus, Stefan and Nikolić, Nađa and Marjanović, Ana and Branković, Marija and Lazović, Vladimir and Tubić, Bojana and Čanak Atlagić, Jelena and Nikolić, Vera and Paunović, Momir",
year = "2019",
abstract = "Trochospongilla horrida Weltner, 1893 (Spongillidae, Spongillida) has a relatively wide, though discontinuous, distribution in the Northern Hemisphere. The aim of this paper was to report the first finding of T. horrida in Serbia. During 2017, research was carried out on ten tributaries of the Sava and the Danube. T. horrida was identified at three localities in Tisa, Juzna Morava and Zapadna Morava rivers, out of 51 inspected sites. For the first time ribosomal 28S DNA was used for T. horrida identification.",
journal = "Limnologica",
title = "First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia – A morphological and genetic study",
volume = "76",
doi = "10.1016/J.LIMNO.2019.03.004",
pages = "48-51"
}

Anđus, S., Nikolić, N., Marjanović, A., Branković, M., Lazović, V., Tubić, B., Čanak Atlagić, J., Nikolić, V.,& Paunović, M.. (2019). First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia – A morphological and genetic study. in Limnologica, 76, 48-51.
https://doi.org/10.1016/J.LIMNO.2019.03.004

Anđus S, Nikolić N, Marjanović A, Branković M, Lazović V, Tubić B, Čanak Atlagić J, Nikolić V, Paunović M. First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia – A morphological and genetic study. in Limnologica. 2019;76:48-51.
doi:10.1016/J.LIMNO.2019.03.004 .

Anđus, Stefan, Nikolić, Nađa, Marjanović, Ana, Branković, Marija, Lazović, Vladimir, Tubić, Bojana, Čanak Atlagić, Jelena, Nikolić, Vera, Paunović, Momir, "First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia – A morphological and genetic study" in Limnologica, 76 (2019):48-51,
https://doi.org/10.1016/J.LIMNO.2019.03.004 . .