TY  - CONF
AU  - Marković, Milan
AU  - Marković, Katarina
AU  - Mirkov, Ivana
AU  - Tucović, Dina
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Popov Aleksandrov, Aleksandra
PY  - 2019
UR  - https://www.isos.rs/congress2019-abstract-book
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3580
UR  - https://radar.ibiss.bg.ac.rs/handle/handle/123456789/3558
AB  - Cadmium (Cd) is one of the most cytotoxic agents and environmental contaminants, which can cause serial health problems in various organs such as liver, kidney, testis, bone, nervous tissue and immune system. Furthermore, Cd is classified as a human and animal carcinogen agent. Despite the fact that Cd accumulates in the skin, there is a lack of evidence about its involvement in skin cancer biology. Skin cancer is one of the most common cancers worldwide, with melanoma as the most lethal type. Based on that, we set up the study to examine the effect of cadmium on the B16 melanoma cell line, measuring viability by MTT and crystal violet (CV) tests, proliferation rate (CFSE), ROS production (DHR), migration (scratch test) and adhesion on matrigel. The results showed that non-cytotoxic doses of cadmium (ranging from 0,3-2,5µM) increased viability, proliferation, and migration of B16 cells, while at the same time slightly promoted ROS production. Otherwise, pronounced oxidative stress induced by higher doses (ranging from 5-20µM) led to cell death. Summary, these results showed that low doses of cadmium could upregulate B16 melanoma cell line growth and migration probably through moderate ROS/RNS generation and thus their ability to modulate relevant signaling pathways involved in cancer progression. Considering that, underlying mechanisms of Cd impact on melanoma cell lines proliferation, migration and invasiveness needs to be clarified in vitro, as well as its relevance for in vivo system.
PB  - Belgrade: University of Belgrade Institute for Biological Research "Siniša Stanković" National Institute of Republic of Serbia
PB  - Belgrade: Immunological Society of Serbia
C3  - Saksida T, Stanisavljević S, Miljković Đ, editors. Immunology at the Confluence of Multidisciplinary Approaches : abstract book; 2019 Dec 6-8; Belgrade, Serbia
T1  - Effect of non-cytotoxic doses of cadmium on the B16 melanoma cell line
SP  - 123
EP  - 123
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3580
ER  - 

@conference{
author = "Marković, Milan and Marković, Katarina and Mirkov, Ivana and Tucović, Dina and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Popov Aleksandrov, Aleksandra",
year = "2019",
abstract = "Cadmium (Cd) is one of the most cytotoxic agents and environmental contaminants, which can cause serial health problems in various organs such as liver, kidney, testis, bone, nervous tissue and immune system. Furthermore, Cd is classified as a human and animal carcinogen agent. Despite the fact that Cd accumulates in the skin, there is a lack of evidence about its involvement in skin cancer biology. Skin cancer is one of the most common cancers worldwide, with melanoma as the most lethal type. Based on that, we set up the study to examine the effect of cadmium on the B16 melanoma cell line, measuring viability by MTT and crystal violet (CV) tests, proliferation rate (CFSE), ROS production (DHR), migration (scratch test) and adhesion on matrigel. The results showed that non-cytotoxic doses of cadmium (ranging from 0,3-2,5µM) increased viability, proliferation, and migration of B16 cells, while at the same time slightly promoted ROS production. Otherwise, pronounced oxidative stress induced by higher doses (ranging from 5-20µM) led to cell death. Summary, these results showed that low doses of cadmium could upregulate B16 melanoma cell line growth and migration probably through moderate ROS/RNS generation and thus their ability to modulate relevant signaling pathways involved in cancer progression. Considering that, underlying mechanisms of Cd impact on melanoma cell lines proliferation, migration and invasiveness needs to be clarified in vitro, as well as its relevance for in vivo system.",
publisher = "Belgrade: University of Belgrade Institute for Biological Research "Siniša Stanković" National Institute of Republic of Serbia, Belgrade: Immunological Society of Serbia",
journal = "Saksida T, Stanisavljević S, Miljković Đ, editors. Immunology at the Confluence of Multidisciplinary Approaches : abstract book; 2019 Dec 6-8; Belgrade, Serbia",
title = "Effect of non-cytotoxic doses of cadmium on the B16 melanoma cell line",
pages = "123-123",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3580"
}

Marković, M., Marković, K., Mirkov, I., Tucović, D., Mijatović, S., Maksimović-Ivanić, D.,& Popov Aleksandrov, A.. (2019). Effect of non-cytotoxic doses of cadmium on the B16 melanoma cell line. in Saksida T, Stanisavljević S, Miljković Đ, editors. Immunology at the Confluence of Multidisciplinary Approaches : abstract book; 2019 Dec 6-8; Belgrade, Serbia
Belgrade: University of Belgrade Institute for Biological Research "Siniša Stanković" National Institute of Republic of Serbia., 123-123.
https://hdl.handle.net/21.15107/rcub_ibiss_3580

Marković M, Marković K, Mirkov I, Tucović D, Mijatović S, Maksimović-Ivanić D, Popov Aleksandrov A. Effect of non-cytotoxic doses of cadmium on the B16 melanoma cell line. in Saksida T, Stanisavljević S, Miljković Đ, editors. Immunology at the Confluence of Multidisciplinary Approaches : abstract book; 2019 Dec 6-8; Belgrade, Serbia. 2019;:123-123.
https://hdl.handle.net/21.15107/rcub_ibiss_3580 .

Marković, Milan, Marković, Katarina, Mirkov, Ivana, Tucović, Dina, Mijatović, Sanja, Maksimović-Ivanić, Danijela, Popov Aleksandrov, Aleksandra, "Effect of non-cytotoxic doses of cadmium on the B16 melanoma cell line" in Saksida T, Stanisavljević S, Miljković Đ, editors. Immunology at the Confluence of Multidisciplinary Approaches : abstract book; 2019 Dec 6-8; Belgrade, Serbia (2019):123-123,
https://hdl.handle.net/21.15107/rcub_ibiss_3580 .