TY  - JOUR
AU  - Matić, Gordana
AU  - Elaković, Ivana
AU  - Vasiljević, Đorđe
AU  - Adžić, Miroslav
AU  - Đorđević, Ana
AU  - Đorđević, Jelena D
AU  - Radojcić, Marija B
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1385
AB  - Gender-related differences in the expression and functional properties of the hepatic glucocorticoid receptor were studied before and after antidepressant fluoxetine administration to both unstressed and rats exposed to a chronic social isolation stress. Some of the receptor's functional properties, including hormone-binding capacity (B(max)), hormone-binding potency (B(max)/K(D) ratio) and the DNA-binding ability, were found to be sexually dimorphic. Fluoxetine treatment (5 mg/kg body mass, 21 day, intraperitoneally) induced a decrease in B(max) and in the amount of Hsp70 co-immunoprecipitated with the glucocorticoid receptor only in males, and stimulated the association of the receptor with Hsp90 in females. When applied during the last three weeks of the 6-week isolation, fluoxetine parallelly elevated B(max) and the receptor protein level in female animals, while in males diminished B(max) and inhibited association of the receptor with Hsp70. Binding of dexamethasone-receptor complexes both to DNA-cellulose and to isolated liver nuclei did not appear to be a target for fluoxetine action. The results point to sex-related differences in the glucocorticoid receptor functioning and in its response to fluoxetine, and suggest that these differences may contribute to well known sexual dimorphism in the sensitivity to stress, to stress-related disorders and to antidepressant treatment. (C) 2010 Elsevier B.V. All rights reserved.
T2  - European Journal of Pharmacology
T1  - Sexually dimorphic functional alterations of rat hepatic glucocorticoid receptor in response to fluoxetine
IS  - 1-3
VL  - 632
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1385
ER  - 

@article{
author = "Matić, Gordana and Elaković, Ivana and Vasiljević, Đorđe and Adžić, Miroslav and Đorđević, Ana and Đorđević, Jelena D and Radojcić, Marija B",
year = "2010",
abstract = "Gender-related differences in the expression and functional properties of the hepatic glucocorticoid receptor were studied before and after antidepressant fluoxetine administration to both unstressed and rats exposed to a chronic social isolation stress. Some of the receptor's functional properties, including hormone-binding capacity (B(max)), hormone-binding potency (B(max)/K(D) ratio) and the DNA-binding ability, were found to be sexually dimorphic. Fluoxetine treatment (5 mg/kg body mass, 21 day, intraperitoneally) induced a decrease in B(max) and in the amount of Hsp70 co-immunoprecipitated with the glucocorticoid receptor only in males, and stimulated the association of the receptor with Hsp90 in females. When applied during the last three weeks of the 6-week isolation, fluoxetine parallelly elevated B(max) and the receptor protein level in female animals, while in males diminished B(max) and inhibited association of the receptor with Hsp70. Binding of dexamethasone-receptor complexes both to DNA-cellulose and to isolated liver nuclei did not appear to be a target for fluoxetine action. The results point to sex-related differences in the glucocorticoid receptor functioning and in its response to fluoxetine, and suggest that these differences may contribute to well known sexual dimorphism in the sensitivity to stress, to stress-related disorders and to antidepressant treatment. (C) 2010 Elsevier B.V. All rights reserved.",
journal = "European Journal of Pharmacology",
title = "Sexually dimorphic functional alterations of rat hepatic glucocorticoid receptor in response to fluoxetine",
number = "1-3",
volume = "632",
pages = "85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1385"
}

Matić, G., Elaković, I., Vasiljević, Đ., Adžić, M., Đorđević, A., Đorđević, J. D.,& Radojcić, M. B.. (2010). Sexually dimorphic functional alterations of rat hepatic glucocorticoid receptor in response to fluoxetine. in European Journal of Pharmacology, 632(1-3).
https://hdl.handle.net/21.15107/rcub_ibiss_1385

Matić G, Elaković I, Vasiljević Đ, Adžić M, Đorđević A, Đorđević JD, Radojcić MB. Sexually dimorphic functional alterations of rat hepatic glucocorticoid receptor in response to fluoxetine. in European Journal of Pharmacology. 2010;632(1-3):null-85.
https://hdl.handle.net/21.15107/rcub_ibiss_1385 .

Matić, Gordana, Elaković, Ivana, Vasiljević, Đorđe, Adžić, Miroslav, Đorđević, Ana, Đorđević, Jelena D, Radojcić, Marija B, "Sexually dimorphic functional alterations of rat hepatic glucocorticoid receptor in response to fluoxetine" in European Journal of Pharmacology, 632, no. 1-3 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1385 .