TY  - JOUR
AU  - Spasojević, Ivan B
AU  - Morina, Arian
AU  - Mojović, Milos D
AU  - Stević, Zorica D
AU  - Spasić, Snezana D
AU  - Jones, David R
AU  - Spasić, Mihajlo
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1405
AB  - A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.
T2  - Redox Report
T1  - Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid
IS  - 1
VL  - 15
EP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1405
ER  - 

@article{
author = "Spasojević, Ivan B and Morina, Arian and Mojović, Milos D and Stević, Zorica D and Spasić, Snezana D and Jones, David R and Spasić, Mihajlo",
year = "2010",
abstract = "A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.",
journal = "Redox Report",
title = "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid",
number = "1",
volume = "15",
pages = "35",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1405"
}

Spasojević, I. B., Morina, A., Mojović, M. D., Stević, Z. D., Spasić, S. D., Jones, D. R.,& Spasić, M.. (2010). Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report, 15(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1405

Spasojević IB, Morina A, Mojović MD, Stević ZD, Spasić SD, Jones DR, Spasić M. Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report. 2010;15(1):null-35.
https://hdl.handle.net/21.15107/rcub_ibiss_1405 .

Spasojević, Ivan B, Morina, Arian, Mojović, Milos D, Stević, Zorica D, Spasić, Snezana D, Jones, David R, Spasić, Mihajlo, "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid" in Redox Report, 15, no. 1 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1405 .

TY  - JOUR
AU  - Oreščanin Dušić, Zorana
AU  - Milovanović, Slobodan R
AU  - Spasić, Snezana D
AU  - Jones, David R
AU  - Spasić, Mihajlo
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1605
AB  - The conversion of nitric oxide (NO*) into its congeners nitrosonium (NO+) and nitroxyl (HNO/NO-) ions may have important consequences for signal transduction and physiological responses. Manganese-containing superoxide dismutase (MnSOD) may convert NO* into its redox congeners. In our current work, we have examined the mechanism of sodium nitroprusside (SNP)-induced relaxation of arteries, with or without endothelium, from both normotensive and spontaneously hypertensive (SH) rats in the absence and presence of MnSOD. SNP induced a greater degree of relaxation in normotensive than in SH rats. MnSOD antagonized SNPinduced relaxation and effect was greater in normotensive than hypertensive rats. However, MnSOD even potentiated SNP-induced relaxation in mesenteric arteries with endothelium from SH rats. Our results indicate that HNO/NO--mediated relaxation is more effective in mesenteric artery smooth muscle from SH rats than from normotensive rats and that vascular dysfunction in SH rats is not solely endothelium-derived but involves changes in vascular smooth muscles.
T2  - Pharmacological Reports
T1  - Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners
IS  - 3
VL  - 59
EP  - 322
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1605
ER  - 

@article{
author = "Oreščanin Dušić, Zorana and Milovanović, Slobodan R and Spasić, Snezana D and Jones, David R and Spasić, Mihajlo",
year = "2007",
abstract = "The conversion of nitric oxide (NO*) into its congeners nitrosonium (NO+) and nitroxyl (HNO/NO-) ions may have important consequences for signal transduction and physiological responses. Manganese-containing superoxide dismutase (MnSOD) may convert NO* into its redox congeners. In our current work, we have examined the mechanism of sodium nitroprusside (SNP)-induced relaxation of arteries, with or without endothelium, from both normotensive and spontaneously hypertensive (SH) rats in the absence and presence of MnSOD. SNP induced a greater degree of relaxation in normotensive than in SH rats. MnSOD antagonized SNPinduced relaxation and effect was greater in normotensive than hypertensive rats. However, MnSOD even potentiated SNP-induced relaxation in mesenteric arteries with endothelium from SH rats. Our results indicate that HNO/NO--mediated relaxation is more effective in mesenteric artery smooth muscle from SH rats than from normotensive rats and that vascular dysfunction in SH rats is not solely endothelium-derived but involves changes in vascular smooth muscles.",
journal = "Pharmacological Reports",
title = "Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners",
number = "3",
volume = "59",
pages = "322",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1605"
}

Oreščanin Dušić, Z., Milovanović, S. R., Spasić, S. D., Jones, D. R.,& Spasić, M.. (2007). Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners. in Pharmacological Reports, 59(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1605

Oreščanin Dušić Z, Milovanović SR, Spasić SD, Jones DR, Spasić M. Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners. in Pharmacological Reports. 2007;59(3):null-322.
https://hdl.handle.net/21.15107/rcub_ibiss_1605 .

Oreščanin Dušić, Zorana, Milovanović, Slobodan R, Spasić, Snezana D, Jones, David R, Spasić, Mihajlo, "Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners" in Pharmacological Reports, 59, no. 3 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1605 .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Niciforović, Ana
AU  - Vucić, Vesna
AU  - Nešković-Konstantinović, Zora B.
AU  - Spasić, Snezana D
AU  - Jones, David R
AU  - Radojcić, Marija B
AU  - Spasić, Mihajlo
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1655
AB  - There is a well-established role for reactive oxygen and nitrogen species, chronic inflammation and immune response in the pathogenesis of breast cancer. Complex interactions between breast cancer cells and surrounding blood vessels are prerequisites for cancer growth and invasion. Reports in the literature concerning the systemic response to, and the effect of, common breast cancer therapy on NF-kappa B and antioxidative defence enzyme expression and activity under clinical conditions are scarce. We determined these parameters in whole blood cell lysate from 16 women with breast cancer before and after combined (cyclophosphamide, doxorubicin, 5-fluorouracil; CAF) therapy and compared the results with 16 healthy women. Significantly higher levels of NF-kappa B and Mn-SOD (both their protein level and their activity) were found in breast cancer patients before and after CAF therapy, in comparison with healthy women. In parallel measurements, no change in the level or activity of catalase (CAT) was detected. According to our findings, it appears that breast cancer creates conditions that increase the level of hydrogen peroxide in the circulating cells and that the applied CAF therapy fails to compensate, therefore creating systemic conditions that favour survival and invasion of breast cancer cells.
T2  - Redox Report
T1  - Systemic NF-kappa B activation in blood cells of breast cancer patients
IS  - 1
VL  - 11
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1655
ER  - 

@article{
author = "Adžić, Miroslav and Niciforović, Ana and Vucić, Vesna and Nešković-Konstantinović, Zora B. and Spasić, Snezana D and Jones, David R and Radojcić, Marija B and Spasić, Mihajlo",
year = "2006",
abstract = "There is a well-established role for reactive oxygen and nitrogen species, chronic inflammation and immune response in the pathogenesis of breast cancer. Complex interactions between breast cancer cells and surrounding blood vessels are prerequisites for cancer growth and invasion. Reports in the literature concerning the systemic response to, and the effect of, common breast cancer therapy on NF-kappa B and antioxidative defence enzyme expression and activity under clinical conditions are scarce. We determined these parameters in whole blood cell lysate from 16 women with breast cancer before and after combined (cyclophosphamide, doxorubicin, 5-fluorouracil; CAF) therapy and compared the results with 16 healthy women. Significantly higher levels of NF-kappa B and Mn-SOD (both their protein level and their activity) were found in breast cancer patients before and after CAF therapy, in comparison with healthy women. In parallel measurements, no change in the level or activity of catalase (CAT) was detected. According to our findings, it appears that breast cancer creates conditions that increase the level of hydrogen peroxide in the circulating cells and that the applied CAF therapy fails to compensate, therefore creating systemic conditions that favour survival and invasion of breast cancer cells.",
journal = "Redox Report",
title = "Systemic NF-kappa B activation in blood cells of breast cancer patients",
number = "1",
volume = "11",
pages = "44",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1655"
}

Adžić, M., Niciforović, A., Vucić, V., Nešković-Konstantinović, Z. B., Spasić, S. D., Jones, D. R., Radojcić, M. B.,& Spasić, M.. (2006). Systemic NF-kappa B activation in blood cells of breast cancer patients. in Redox Report, 11(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1655

Adžić M, Niciforović A, Vucić V, Nešković-Konstantinović ZB, Spasić SD, Jones DR, Radojcić MB, Spasić M. Systemic NF-kappa B activation in blood cells of breast cancer patients. in Redox Report. 2006;11(1):null-44.
https://hdl.handle.net/21.15107/rcub_ibiss_1655 .

Adžić, Miroslav, Niciforović, Ana, Vucić, Vesna, Nešković-Konstantinović, Zora B., Spasić, Snezana D, Jones, David R, Radojcić, Marija B, Spasić, Mihajlo, "Systemic NF-kappa B activation in blood cells of breast cancer patients" in Redox Report, 11, no. 1 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1655 .