TY  - JOUR
AU  - Miljković, Danijela
AU  - Selaković, Sara
AU  - Vujić, Vukica
AU  - Stanisavljević, Nemanja
AU  - Radović, Svetlana
AU  - Cvetković, Dragana
PY  - 2018
UR  - http://link.springer.com/10.1007/s00035-018-0203-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3040
AB  - Light environments can influence variation in plant morphology, development and susceptibility to herbivores. Our research interest was to investigate the patterns of herbivore damage and developmental stability in dioecious understory forb Mercurialis perennis in contrasting light habitats, located at 1700 m a.s.l. on Mt. Kopaonik. Male and female plants from two light habitats, open (a sun-exposed field) and shaded (a spruce forest) were examined with respect to: herbivore damage (percentage of leaf area loss), fluctuating asymetry (FA) as a measurement of developmental stability, plant morphological and, specifically, leaf size traits, as well as biochemical traits relating to nutritional quality and defence, taking into account the possible presence of intersexual differences. Our results show that herbivore damage was significantly higher in open habitat, as well as one out of four univariate FA indices and the multivariate index. Morphological and biochemical traits, apart from defensive compounds, had higher values in the shade, pointing to sun-exposed habitat being more stressful for this species. Intersexual differences were observed for foliar damage, defensive compounds (phenolics and tannins), all leaf size traits, total leaf area, and protein content. Contrasting light habitats affected most of the analysed traits. Both foliar damage and FA were higher in a more stressful habitat; within habitats, no positive correlations were found. Herbivore damage was significantly male biased in open habitat. The analysis of intersexual differences in developmental stability measured by leaf asymmetry levels provided no evidence that female plants were more sensitive to environmental stress.
T2  - Alpine Botany
T1  - Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats
IS  - 2
VL  - 128
DO  - 10.1007/s00035-018-0203-8
SP  - 193
EP  - 206
ER  - 

@article{
author = "Miljković, Danijela and Selaković, Sara and Vujić, Vukica and Stanisavljević, Nemanja and Radović, Svetlana and Cvetković, Dragana",
year = "2018",
abstract = "Light environments can influence variation in plant morphology, development and susceptibility to herbivores. Our research interest was to investigate the patterns of herbivore damage and developmental stability in dioecious understory forb Mercurialis perennis in contrasting light habitats, located at 1700 m a.s.l. on Mt. Kopaonik. Male and female plants from two light habitats, open (a sun-exposed field) and shaded (a spruce forest) were examined with respect to: herbivore damage (percentage of leaf area loss), fluctuating asymetry (FA) as a measurement of developmental stability, plant morphological and, specifically, leaf size traits, as well as biochemical traits relating to nutritional quality and defence, taking into account the possible presence of intersexual differences. Our results show that herbivore damage was significantly higher in open habitat, as well as one out of four univariate FA indices and the multivariate index. Morphological and biochemical traits, apart from defensive compounds, had higher values in the shade, pointing to sun-exposed habitat being more stressful for this species. Intersexual differences were observed for foliar damage, defensive compounds (phenolics and tannins), all leaf size traits, total leaf area, and protein content. Contrasting light habitats affected most of the analysed traits. Both foliar damage and FA were higher in a more stressful habitat; within habitats, no positive correlations were found. Herbivore damage was significantly male biased in open habitat. The analysis of intersexual differences in developmental stability measured by leaf asymmetry levels provided no evidence that female plants were more sensitive to environmental stress.",
journal = "Alpine Botany",
title = "Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats",
number = "2",
volume = "128",
doi = "10.1007/s00035-018-0203-8",
pages = "193-206"
}

Miljković, D., Selaković, S., Vujić, V., Stanisavljević, N., Radović, S.,& Cvetković, D.. (2018). Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats. in Alpine Botany, 128(2), 193-206.
https://doi.org/10.1007/s00035-018-0203-8

Miljković D, Selaković S, Vujić V, Stanisavljević N, Radović S, Cvetković D. Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats. in Alpine Botany. 2018;128(2):193-206.
doi:10.1007/s00035-018-0203-8 .

Miljković, Danijela, Selaković, Sara, Vujić, Vukica, Stanisavljević, Nemanja, Radović, Svetlana, Cvetković, Dragana, "Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats" in Alpine Botany, 128, no. 2 (2018):193-206,
https://doi.org/10.1007/s00035-018-0203-8 . .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Stanisavljević, Nemanja
AU  - Miljuš-Đukić, Jovanka
AU  - Marin, Marija
AU  - Radović, Svetlana
AU  - Ninković, Slavica
PY  - 2018
UR  - http://link.springer.com/10.1007/s00709-018-1250-0
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3064
AB  - Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataša seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.
T2  - Protoplasma
T1  - Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.
DO  - 10.1007/s00709-018-1250-0
ER  - 

@article{
author = "Banjac, Nevena and Stanišić, Mariana and Savić, Jelena and Ćosić, Tatjana and Stanisavljević, Nemanja and Miljuš-Đukić, Jovanka and Marin, Marija and Radović, Svetlana and Ninković, Slavica",
year = "2018",
abstract = "Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataša seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.",
journal = "Protoplasma",
title = "Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.",
doi = "10.1007/s00709-018-1250-0"
}

Banjac, N., Stanišić, M., Savić, J., Ćosić, T., Stanisavljević, N., Miljuš-Đukić, J., Marin, M., Radović, S.,& Ninković, S.. (2018). Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.. in Protoplasma.
https://doi.org/10.1007/s00709-018-1250-0

Banjac N, Stanišić M, Savić J, Ćosić T, Stanisavljević N, Miljuš-Đukić J, Marin M, Radović S, Ninković S. Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.. in Protoplasma. 2018;.
doi:10.1007/s00709-018-1250-0 .

Banjac, Nevena, Stanišić, Mariana, Savić, Jelena, Ćosić, Tatjana, Stanisavljević, Nemanja, Miljuš-Đukić, Jovanka, Marin, Marija, Radović, Svetlana, Ninković, Slavica, "Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate." in Protoplasma (2018),
https://doi.org/10.1007/s00709-018-1250-0 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Savić, Jelena
AU  - Jovanović, Živko
AU  - Miljuš-Đukić, Jovanka
AU  - Senćanski, Jelena
AU  - Simonović, Mladen
AU  - Radović, Svetlana
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2018
UR  - http://link.springer.com/10.1007/s11738-018-2677-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3057
AB  - This study investigated the role of antioxidant system of Alyssum markgrafii, during long-term exposure to 0.5 or 1 mM NiCl2 × 6H2O in vitro. Applied methodology included sample preparation protocol which reduces oxidation of key metabolites along with novel luminescent method and well-established photometric procedures. During 5-week treatments, plants accumulated 1121 and 2470 ppm of Ni2+ respectively, followed by severe growth retardation, chlorophyll degradation and peroxidation of lipids. These effects were more pronounced after 1 mM Ni2+ treatment and additionally accompanied by increased water loss. Activities of luminol-converting peroxidases and glutathione reductase upon 0.5 mM treatment were increased while catalase and superoxide dismutase were diminished. The fact that these two groups of enzymes run in antiparallel might suggest functional redistribution between antioxidant enzymes rather than orchestrated action to prevent oxidative damage. Total antioxidant capacity (TAC) was also increased after 0.5 mM treatment which coincided with increased GR activity and elevated glutathione content indicating this low molecular weight antioxidant as an important factor associated with nickel tolerance. This study also emphasizes the possible important role of luminol-converting peroxidases in nickel hyperaccumulation, although they are not considered as antioxidant enzymes sensu stricto since some of them can also produce reactive oxygen species as well.
T2  - Acta Physiologiae Plantarum
T1  - Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro
IS  - 6
VL  - 40
DO  - 10.1007/s11738-018-2677-9
SP  - 101
ER  - 

@article{
author = "Stanisavljević, Nemanja and Savić, Jelena and Jovanović, Živko and Miljuš-Đukić, Jovanka and Senćanski, Jelena and Simonović, Mladen and Radović, Svetlana and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2018",
abstract = "This study investigated the role of antioxidant system of Alyssum markgrafii, during long-term exposure to 0.5 or 1 mM NiCl2 × 6H2O in vitro. Applied methodology included sample preparation protocol which reduces oxidation of key metabolites along with novel luminescent method and well-established photometric procedures. During 5-week treatments, plants accumulated 1121 and 2470 ppm of Ni2+ respectively, followed by severe growth retardation, chlorophyll degradation and peroxidation of lipids. These effects were more pronounced after 1 mM Ni2+ treatment and additionally accompanied by increased water loss. Activities of luminol-converting peroxidases and glutathione reductase upon 0.5 mM treatment were increased while catalase and superoxide dismutase were diminished. The fact that these two groups of enzymes run in antiparallel might suggest functional redistribution between antioxidant enzymes rather than orchestrated action to prevent oxidative damage. Total antioxidant capacity (TAC) was also increased after 0.5 mM treatment which coincided with increased GR activity and elevated glutathione content indicating this low molecular weight antioxidant as an important factor associated with nickel tolerance. This study also emphasizes the possible important role of luminol-converting peroxidases in nickel hyperaccumulation, although they are not considered as antioxidant enzymes sensu stricto since some of them can also produce reactive oxygen species as well.",
journal = "Acta Physiologiae Plantarum",
title = "Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro",
number = "6",
volume = "40",
doi = "10.1007/s11738-018-2677-9",
pages = "101"
}

Stanisavljević, N., Savić, J., Jovanović, Ž., Miljuš-Đukić, J., Senćanski, J., Simonović, M., Radović, S., Vinterhalter, D.,& Vinterhalter, B.. (2018). Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro. in Acta Physiologiae Plantarum, 40(6), 101.
https://doi.org/10.1007/s11738-018-2677-9

Stanisavljević N, Savić J, Jovanović Ž, Miljuš-Đukić J, Senćanski J, Simonović M, Radović S, Vinterhalter D, Vinterhalter B. Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro. in Acta Physiologiae Plantarum. 2018;40(6):101.
doi:10.1007/s11738-018-2677-9 .

Stanisavljević, Nemanja, Savić, Jelena, Jovanović, Živko, Miljuš-Đukić, Jovanka, Senćanski, Jelena, Simonović, Mladen, Radović, Svetlana, Vinterhalter, Dragan, Vinterhalter, Branka, "Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro" in Acta Physiologiae Plantarum, 40, no. 6 (2018):101,
https://doi.org/10.1007/s11738-018-2677-9 . .

TY  - CONF
AU  - Ninković, Slavica
AU  - Nikolić, Radomirka
AU  - Banjac, Nevena
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Ninković, Sofija
AU  - Raspor, Martin
AU  - Ćosić, Tatjana
AU  - Radović, Svetlana
AU  - Smigocki, Ann C.
PY  - 2014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5595
AB  - Proteinase inhibitors (Pls) are small proteins that occur naturally in a number of plant species that are
characterized by varied specificity toward proteinases, among them insect digestive proteinases of the
serine, cysteine, aspartyl and meialloproteinase class. Over-expression of heterologous proteinase
inhibitor genes in transgenic plants was used as a strategy for introducing insect and nematode
control. Besides their main effects (biodefense), Pls are also known to have positive side-effects on
transgenic plant physiology. ln this study engineered bird's foot trefoil (Lofus corniculatus L.) plants
with three different PI genes - Beta vulgaris serine Pl, Nicotiana alata serine Pl and squash aspartyl Pl
- exhibited enhanced growth with higher number of tillers and increased fresh and dry weight.
Moreover, some of the transformed lines displayed higher tolerance to NaCl stress evaluated on the
basis of growth and associated parameters, such as salinity injury and chlorophyll content, using two
NaCl levels (100 and 200 mM) for up to 10 d. Overall, the results indicate that the over-expression of
heterologous proteinase inhibitors could be a promising approach for increasing productivity of
genetically improved Lotus corniculatus L. plants.
PB  - International Association for Plant Biotechnology
C3  - Book of abstracts: International Association for Plant Biotechnology Congress 2014; 2014 Aug 10-15; Melbourne, Australia
T1  - Pleiotropic effects of over-expressed heterologous proteinase inhibitors in transformed Lotus corniculatus L. plants
SP  - 246
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5595
ER  - 

@conference{
author = "Ninković, Slavica and Nikolić, Radomirka and Banjac, Nevena and Cingel, Aleksandar and Savić, Jelena and Ninković, Sofija and Raspor, Martin and Ćosić, Tatjana and Radović, Svetlana and Smigocki, Ann C.",
year = "2014",
abstract = "Proteinase inhibitors (Pls) are small proteins that occur naturally in a number of plant species that are
characterized by varied specificity toward proteinases, among them insect digestive proteinases of the
serine, cysteine, aspartyl and meialloproteinase class. Over-expression of heterologous proteinase
inhibitor genes in transgenic plants was used as a strategy for introducing insect and nematode
control. Besides their main effects (biodefense), Pls are also known to have positive side-effects on
transgenic plant physiology. ln this study engineered bird's foot trefoil (Lofus corniculatus L.) plants
with three different PI genes - Beta vulgaris serine Pl, Nicotiana alata serine Pl and squash aspartyl Pl
- exhibited enhanced growth with higher number of tillers and increased fresh and dry weight.
Moreover, some of the transformed lines displayed higher tolerance to NaCl stress evaluated on the
basis of growth and associated parameters, such as salinity injury and chlorophyll content, using two
NaCl levels (100 and 200 mM) for up to 10 d. Overall, the results indicate that the over-expression of
heterologous proteinase inhibitors could be a promising approach for increasing productivity of
genetically improved Lotus corniculatus L. plants.",
publisher = "International Association for Plant Biotechnology",
journal = "Book of abstracts: International Association for Plant Biotechnology Congress 2014; 2014 Aug 10-15; Melbourne, Australia",
title = "Pleiotropic effects of over-expressed heterologous proteinase inhibitors in transformed Lotus corniculatus L. plants",
pages = "246",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5595"
}

Ninković, S., Nikolić, R., Banjac, N., Cingel, A., Savić, J., Ninković, S., Raspor, M., Ćosić, T., Radović, S.,& Smigocki, A. C.. (2014). Pleiotropic effects of over-expressed heterologous proteinase inhibitors in transformed Lotus corniculatus L. plants. in Book of abstracts: International Association for Plant Biotechnology Congress 2014; 2014 Aug 10-15; Melbourne, Australia
International Association for Plant Biotechnology., 246.
https://hdl.handle.net/21.15107/rcub_ibiss_5595

Ninković S, Nikolić R, Banjac N, Cingel A, Savić J, Ninković S, Raspor M, Ćosić T, Radović S, Smigocki AC. Pleiotropic effects of over-expressed heterologous proteinase inhibitors in transformed Lotus corniculatus L. plants. in Book of abstracts: International Association for Plant Biotechnology Congress 2014; 2014 Aug 10-15; Melbourne, Australia. 2014;:246.
https://hdl.handle.net/21.15107/rcub_ibiss_5595 .

Ninković, Slavica, Nikolić, Radomirka, Banjac, Nevena, Cingel, Aleksandar, Savić, Jelena, Ninković, Sofija, Raspor, Martin, Ćosić, Tatjana, Radović, Svetlana, Smigocki, Ann C., "Pleiotropic effects of over-expressed heterologous proteinase inhibitors in transformed Lotus corniculatus L. plants" in Book of abstracts: International Association for Plant Biotechnology Congress 2014; 2014 Aug 10-15; Melbourne, Australia (2014):246,
https://hdl.handle.net/21.15107/rcub_ibiss_5595 .

TY  - JOUR
AU  - Stanisavljević, Nemanja S
AU  - Savić, Jelena M.
AU  - Jovanović, Zivko S
AU  - Miljuš-Đukić, Jovanka D.
AU  - Radović, Svetlana R
AU  - Vinterhalter, Dragan V
AU  - Vinterhalter, Branka
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1142
AB  - Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effects of excess nickel were observed through slower growth and biomass decrease, together with increased reactive oxygen species (ROS) production and lipid peroxidation. Nickel exposure decreased chlorophyll a, b, total chlorophyll as well as carotenoid concentration. Addition of sodium benzoate, potent ROS scavenger, showed concentration-dependent disturbing effect on nickel hyperaccumulation, lowering the content of accumulated nickel in A. markgrafii shoots. General reducing power represented by low molecular weight antioxidants and phenols was inversely correlated with nickel concentration. Among the investigated antioxidative enzymes, POD seems to play important role in ROS level regulation upon excessive nickel in medium.
T2  - Acta Physiologiae Plantarum
T1  - Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level
IS  - 5
VL  - 34
SP  - 339
EP  - 2006
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1142
ER  - 

@article{
author = "Stanisavljević, Nemanja S and Savić, Jelena M. and Jovanović, Zivko S and Miljuš-Đukić, Jovanka D. and Radović, Svetlana R and Vinterhalter, Dragan V and Vinterhalter, Branka",
year = "2012",
abstract = "Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effects of excess nickel were observed through slower growth and biomass decrease, together with increased reactive oxygen species (ROS) production and lipid peroxidation. Nickel exposure decreased chlorophyll a, b, total chlorophyll as well as carotenoid concentration. Addition of sodium benzoate, potent ROS scavenger, showed concentration-dependent disturbing effect on nickel hyperaccumulation, lowering the content of accumulated nickel in A. markgrafii shoots. General reducing power represented by low molecular weight antioxidants and phenols was inversely correlated with nickel concentration. Among the investigated antioxidative enzymes, POD seems to play important role in ROS level regulation upon excessive nickel in medium.",
journal = "Acta Physiologiae Plantarum",
title = "Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level",
number = "5",
volume = "34",
pages = "339-2006",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1142"
}

Stanisavljević, N. S., Savić, J. M., Jovanović, Z. S., Miljuš-Đukić, J. D., Radović, S. R., Vinterhalter, D. V.,& Vinterhalter, B.. (2012). Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level. in Acta Physiologiae Plantarum, 34(5), 339-2006.
https://hdl.handle.net/21.15107/rcub_ibiss_1142

Stanisavljević NS, Savić JM, Jovanović ZS, Miljuš-Đukić JD, Radović SR, Vinterhalter DV, Vinterhalter B. Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level. in Acta Physiologiae Plantarum. 2012;34(5):339-2006.
https://hdl.handle.net/21.15107/rcub_ibiss_1142 .

Stanisavljević, Nemanja S, Savić, Jelena M., Jovanović, Zivko S, Miljuš-Đukić, Jovanka D., Radović, Svetlana R, Vinterhalter, Dragan V, Vinterhalter, Branka, "Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level" in Acta Physiologiae Plantarum, 34, no. 5 (2012):339-2006,
https://hdl.handle.net/21.15107/rcub_ibiss_1142 .

TY  - JOUR
AU  - Ninković, Slavica
AU  - Ćosić, Tatjana
AU  - Vinterhalter, Branka
AU  - Uzelac, Branka
AU  - Cingel, Aleksandar
AU  - Savić, Jelena M.
AU  - Radović, Svetlana R
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1340
AB  - Agrobacterium rhizogenes A4M70GUS-mediated transformation of two local breeding lines of sugar beet was obtained using 4-week-old seedlings. Root formation efficiency was 61.54% for SBa genotype and 36.36% for SBb genotype. Five highly proliferated hairy root lines have been established in liquid hormone-free MS medium. Transgenic nature of the hairy root clones was evaluated by GUS assay, PCR and RT-PCR analyses. Hairy root-derived calli were induced using different plant growth regulators (PGRs): auxin, auxin/cytokinin and cytokinin. The best callus induction response was achieved on MS medium containing both 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l thidiazuron (TDZ). Globular embryo-like structures were observed in friable callus after its prolonged cultivation on MS medium supplemented with TDZ and giberellic acid (GA(3)) at 1 mg/l each, followed by growth on MS medium containing 1% glucose and 0.5 mg/l 2,3,5-triiodobenzoic acid (TIBA). Histological analysis revealed somatic embryos at different stages of development in hairy root-derived callus of sugar beet.
T2  - Plant Cell Tissue and Organ Culture
T1  - Embryogenic responses of Beta vulgaris L. callus induced from transgenic hairy roots
IS  - 1
VL  - 103
EP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1340
ER  - 

@article{
author = "Ninković, Slavica and Ćosić, Tatjana and Vinterhalter, Branka and Uzelac, Branka and Cingel, Aleksandar and Savić, Jelena M. and Radović, Svetlana R",
year = "2010",
abstract = "Agrobacterium rhizogenes A4M70GUS-mediated transformation of two local breeding lines of sugar beet was obtained using 4-week-old seedlings. Root formation efficiency was 61.54% for SBa genotype and 36.36% for SBb genotype. Five highly proliferated hairy root lines have been established in liquid hormone-free MS medium. Transgenic nature of the hairy root clones was evaluated by GUS assay, PCR and RT-PCR analyses. Hairy root-derived calli were induced using different plant growth regulators (PGRs): auxin, auxin/cytokinin and cytokinin. The best callus induction response was achieved on MS medium containing both 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l thidiazuron (TDZ). Globular embryo-like structures were observed in friable callus after its prolonged cultivation on MS medium supplemented with TDZ and giberellic acid (GA(3)) at 1 mg/l each, followed by growth on MS medium containing 1% glucose and 0.5 mg/l 2,3,5-triiodobenzoic acid (TIBA). Histological analysis revealed somatic embryos at different stages of development in hairy root-derived callus of sugar beet.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Embryogenic responses of Beta vulgaris L. callus induced from transgenic hairy roots",
number = "1",
volume = "103",
pages = "91",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1340"
}

Ninković, S., Ćosić, T., Vinterhalter, B., Uzelac, B., Cingel, A., Savić, J. M.,& Radović, S. R.. (2010). Embryogenic responses of Beta vulgaris L. callus induced from transgenic hairy roots. in Plant Cell Tissue and Organ Culture, 103(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1340

Ninković S, Ćosić T, Vinterhalter B, Uzelac B, Cingel A, Savić JM, Radović SR. Embryogenic responses of Beta vulgaris L. callus induced from transgenic hairy roots. in Plant Cell Tissue and Organ Culture. 2010;103(1):null-91.
https://hdl.handle.net/21.15107/rcub_ibiss_1340 .

Ninković, Slavica, Ćosić, Tatjana, Vinterhalter, Branka, Uzelac, Branka, Cingel, Aleksandar, Savić, Jelena M., Radović, Svetlana R, "Embryogenic responses of Beta vulgaris L. callus induced from transgenic hairy roots" in Plant Cell Tissue and Organ Culture, 103, no. 1 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1340 .

TY  - JOUR
AU  - Miljuš-Đukić, Jovanka D.
AU  - Ninković, Slavica
AU  - Radović, Svetlana R
AU  - Maksimović, Vesna R
AU  - Brkljačić, Jelena M.
AU  - Nešković, Mirjana
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1748
AB  - Buckwheat ( Fagopyrum esculentum Moench) is a heterostylous plant displaying heteromorphic sporophytic self-incompatibility (SI). In order to detect proteins involved in SI, pistils from both long and short styles were isolated and then selfed or cross-pollinated. One-dimensional gel electrophoresis revealed that short pistils 2 h after selfing contained an unique 50 kDa protein. In the two-dimensional electrophoresis two distinct groups of proteins possibly involved in SI response were detected in the short, and one in the long pistils.
T2  - Biologia Plantarum
T1  - Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat
IS  - 2
VL  - 48
EP  - 296
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1748
ER  - 

@article{
author = "Miljuš-Đukić, Jovanka D. and Ninković, Slavica and Radović, Svetlana R and Maksimović, Vesna R and Brkljačić, Jelena M. and Nešković, Mirjana",
year = "2004",
abstract = "Buckwheat ( Fagopyrum esculentum Moench) is a heterostylous plant displaying heteromorphic sporophytic self-incompatibility (SI). In order to detect proteins involved in SI, pistils from both long and short styles were isolated and then selfed or cross-pollinated. One-dimensional gel electrophoresis revealed that short pistils 2 h after selfing contained an unique 50 kDa protein. In the two-dimensional electrophoresis two distinct groups of proteins possibly involved in SI response were detected in the short, and one in the long pistils.",
journal = "Biologia Plantarum",
title = "Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat",
number = "2",
volume = "48",
pages = "296",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1748"
}

Miljuš-Đukić, J. D., Ninković, S., Radović, S. R., Maksimović, V. R., Brkljačić, J. M.,& Nešković, M.. (2004). Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat. in Biologia Plantarum, 48(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1748

Miljuš-Đukić JD, Ninković S, Radović SR, Maksimović VR, Brkljačić JM, Nešković M. Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat. in Biologia Plantarum. 2004;48(2):null-296.
https://hdl.handle.net/21.15107/rcub_ibiss_1748 .

Miljuš-Đukić, Jovanka D., Ninković, Slavica, Radović, Svetlana R, Maksimović, Vesna R, Brkljačić, Jelena M., Nešković, Mirjana, "Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat" in Biologia Plantarum, 48, no. 2 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1748 .