TY  - JOUR
AU  - Adžić, Miroslav
AU  - Lukić, Iva
AU  - Mitić, Milos Z
AU  - Đorđević, Jelena D
AU  - Elaković, Ivana
AU  - Đorđević, Ana
AU  - Krstić-Demonacos, Marija
AU  - Matić, Gordana
AU  - Radojcić, Marija B
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/940
AB  - Antidepressants affect glucocorticoid receptor (GR) functioning partly through modulation of its phosphorylation but their effects on mitochondrial GR have remained undefined. We investigated the ability of chronic fiuoxetine treatment to affect chronic stress-induced changes of mitochondrial GR and its phosphoisoforms (pGRs) in the prefrontal cortex and hippocampus of female and male rats. Since mitochondrial GR regulates oxidative phosphorylation, expression of mitochondrial-encoded subunits of cytochrome (cyt) c oxidase and its activity were also investigated. Chronic stress caused accumulation of the GR in mitochondria of female prefrontal cortex, while the changes in the hippocampus were sex-specific at the levels of pGRs. Expression of mitochondrial COXs genes corresponded to chronic stress-modulated mitochondrial GR in both tissues of both genders and to cyt c oxidase activity in females. Moreover, the metabolic parameters in stressed animals were affected by fiuoxetine therapy only in the hippocampus. Namely, fluoxetine effects on mitochondrial COXs and cyt c oxidase activity in the hippocampus seem to be conveyed through pGR232 in females, while in males this likely occurs through other mechanisms. In summary, sex-specific regulation of cyt c oxidase by the stress and antidepressant treatment and its differential convergence with mitochondrial GR signaling in the prefrontal cortex and hippocampus could contribute to clarification of sex-dependent vulnerability to stress-related disorders and sex-specific clinical impact of antidepressants. (C) 2013 Elsevier Ltd. All rights reserved.
T2  - Psychoneuroendocrinology
T1  - Brain region- and sex-specific modulation of mitochondrial glucocorticoid receptor phosphorylation in fluoxetine treated stressed rats: Effects on energy metabolism
IS  - 12
VL  - 38
SP  - 1459
EP  - 2924
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_940
ER  - 

@article{
author = "Adžić, Miroslav and Lukić, Iva and Mitić, Milos Z and Đorđević, Jelena D and Elaković, Ivana and Đorđević, Ana and Krstić-Demonacos, Marija and Matić, Gordana and Radojcić, Marija B",
year = "2013",
abstract = "Antidepressants affect glucocorticoid receptor (GR) functioning partly through modulation of its phosphorylation but their effects on mitochondrial GR have remained undefined. We investigated the ability of chronic fiuoxetine treatment to affect chronic stress-induced changes of mitochondrial GR and its phosphoisoforms (pGRs) in the prefrontal cortex and hippocampus of female and male rats. Since mitochondrial GR regulates oxidative phosphorylation, expression of mitochondrial-encoded subunits of cytochrome (cyt) c oxidase and its activity were also investigated. Chronic stress caused accumulation of the GR in mitochondria of female prefrontal cortex, while the changes in the hippocampus were sex-specific at the levels of pGRs. Expression of mitochondrial COXs genes corresponded to chronic stress-modulated mitochondrial GR in both tissues of both genders and to cyt c oxidase activity in females. Moreover, the metabolic parameters in stressed animals were affected by fiuoxetine therapy only in the hippocampus. Namely, fluoxetine effects on mitochondrial COXs and cyt c oxidase activity in the hippocampus seem to be conveyed through pGR232 in females, while in males this likely occurs through other mechanisms. In summary, sex-specific regulation of cyt c oxidase by the stress and antidepressant treatment and its differential convergence with mitochondrial GR signaling in the prefrontal cortex and hippocampus could contribute to clarification of sex-dependent vulnerability to stress-related disorders and sex-specific clinical impact of antidepressants. (C) 2013 Elsevier Ltd. All rights reserved.",
journal = "Psychoneuroendocrinology",
title = "Brain region- and sex-specific modulation of mitochondrial glucocorticoid receptor phosphorylation in fluoxetine treated stressed rats: Effects on energy metabolism",
number = "12",
volume = "38",
pages = "1459-2924",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_940"
}

Adžić, M., Lukić, I., Mitić, M. Z., Đorđević, J. D., Elaković, I., Đorđević, A., Krstić-Demonacos, M., Matić, G.,& Radojcić, M. B.. (2013). Brain region- and sex-specific modulation of mitochondrial glucocorticoid receptor phosphorylation in fluoxetine treated stressed rats: Effects on energy metabolism. in Psychoneuroendocrinology, 38(12), 1459-2924.
https://hdl.handle.net/21.15107/rcub_ibiss_940

Adžić M, Lukić I, Mitić MZ, Đorđević JD, Elaković I, Đorđević A, Krstić-Demonacos M, Matić G, Radojcić MB. Brain region- and sex-specific modulation of mitochondrial glucocorticoid receptor phosphorylation in fluoxetine treated stressed rats: Effects on energy metabolism. in Psychoneuroendocrinology. 2013;38(12):1459-2924.
https://hdl.handle.net/21.15107/rcub_ibiss_940 .

Adžić, Miroslav, Lukić, Iva, Mitić, Milos Z, Đorđević, Jelena D, Elaković, Ivana, Đorđević, Ana, Krstić-Demonacos, Marija, Matić, Gordana, Radojcić, Marija B, "Brain region- and sex-specific modulation of mitochondrial glucocorticoid receptor phosphorylation in fluoxetine treated stressed rats: Effects on energy metabolism" in Psychoneuroendocrinology, 38, no. 12 (2013):1459-2924,
https://hdl.handle.net/21.15107/rcub_ibiss_940 .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Đorđević, Jelena D
AU  - Mitić, Milos Z
AU  - Simić, Iva
AU  - Rackov, Gorjana
AU  - Đorđević, Ana
AU  - Elaković, Ivana
AU  - Matić, Gordana
AU  - Radojcić, Marija B
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1319
AB  - Alterations in the antioxidative defense parameters upon chronic stress are considered critical for pathophysiology of stress related psychiatric disorders, and their status in blood serves as biomarker for effects of pharmacological treatments. The present study was designed to investigate the modulation of erythrocyte antioxidant enzymes (AOEs): CuZn superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GLR) activities and their protein expression in Wistar male rats subjected to chronic psychosocial isolation and/or to pharmacological treatment with fluoxetine. Chronically isolated animals exhibited decreased levels of serum corticosterone, as opposed to other chronic stress paradigms. In addition to that, SOD, CAT and GPx status was not altered either by chronic psychosocial isolation or by fluoxetine treatment. In contrast, GLR activity and its protein level were both markedly reduced by fluoxetine. Since, GLR is crucial for overall oxido-reductive balance through maintaining optimal ratio of reduced/oxidized glutathione level (GSH/GSSG) in erythrocytes, these results could indicate that in spite of numerous beneficial effects of fluoxetine, it may compromise both haemoglobin function and oxygen transport.
T2  - Acta Chimica Slovenica
T1  - Fluoxetine Decreases Glutathione Reductase in Erythrocytes of Chronically Isolated Wistar Rats
IS  - 4
VL  - 58
EP  - 791
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1319
ER  - 

@article{
author = "Adžić, Miroslav and Đorđević, Jelena D and Mitić, Milos Z and Simić, Iva and Rackov, Gorjana and Đorđević, Ana and Elaković, Ivana and Matić, Gordana and Radojcić, Marija B",
year = "2011",
abstract = "Alterations in the antioxidative defense parameters upon chronic stress are considered critical for pathophysiology of stress related psychiatric disorders, and their status in blood serves as biomarker for effects of pharmacological treatments. The present study was designed to investigate the modulation of erythrocyte antioxidant enzymes (AOEs): CuZn superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GLR) activities and their protein expression in Wistar male rats subjected to chronic psychosocial isolation and/or to pharmacological treatment with fluoxetine. Chronically isolated animals exhibited decreased levels of serum corticosterone, as opposed to other chronic stress paradigms. In addition to that, SOD, CAT and GPx status was not altered either by chronic psychosocial isolation or by fluoxetine treatment. In contrast, GLR activity and its protein level were both markedly reduced by fluoxetine. Since, GLR is crucial for overall oxido-reductive balance through maintaining optimal ratio of reduced/oxidized glutathione level (GSH/GSSG) in erythrocytes, these results could indicate that in spite of numerous beneficial effects of fluoxetine, it may compromise both haemoglobin function and oxygen transport.",
journal = "Acta Chimica Slovenica",
title = "Fluoxetine Decreases Glutathione Reductase in Erythrocytes of Chronically Isolated Wistar Rats",
number = "4",
volume = "58",
pages = "791",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1319"
}

Adžić, M., Đorđević, J. D., Mitić, M. Z., Simić, I., Rackov, G., Đorđević, A., Elaković, I., Matić, G.,& Radojcić, M. B.. (2011). Fluoxetine Decreases Glutathione Reductase in Erythrocytes of Chronically Isolated Wistar Rats. in Acta Chimica Slovenica, 58(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1319

Adžić M, Đorđević JD, Mitić MZ, Simić I, Rackov G, Đorđević A, Elaković I, Matić G, Radojcić MB. Fluoxetine Decreases Glutathione Reductase in Erythrocytes of Chronically Isolated Wistar Rats. in Acta Chimica Slovenica. 2011;58(4):null-791.
https://hdl.handle.net/21.15107/rcub_ibiss_1319 .

Adžić, Miroslav, Đorđević, Jelena D, Mitić, Milos Z, Simić, Iva, Rackov, Gorjana, Đorđević, Ana, Elaković, Ivana, Matić, Gordana, Radojcić, Marija B, "Fluoxetine Decreases Glutathione Reductase in Erythrocytes of Chronically Isolated Wistar Rats" in Acta Chimica Slovenica, 58, no. 4 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1319 .