TY  - CONF
AU  - Simonović, Milica
AU  - Nestorović Živković, Jasmina
AU  - Bjedov, Ivana
AU  - Nešić, Marija
AU  - Jovanović, Vladan
AU  - Giba, Zlatko
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Dmitrović, Slavica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5569
AB  - The use of bioherbicides offers the possibility to suppress harmful weeds by ecologically acceptable methods and thus increase crop productivity. Progress in understanding the mechanisms of bioherbicides action and weed defense response mechanisms, could help in defining
novel weed control strategies. This work highlights the bioherbicide potential of rhizomes of Sorghum halepense L., characterized by a high content of phenolic acids, especially p-hydroxybenzoic
acid (pHB A) and chlorogenic acid (CHL A). Methanol extract of S. halepense rhizome (SHR), and its
major bioactive principles, displayed phytotoxic effects against Chenopodium murale L., which is a
common weed of fields and roadsides. Besides reduction in C. murale seed germination, rhizome
extract of S. halepense applied in the concentrations of 0.01, 0.1, 1 and 2 mg/ml, induced changes
in the activity of antioxidant enzymes in seedlings. Catalase (CAT) activity was increased, while
peroxidase (POX) and superoxide dismutase (SOD) activities were generally decreased, with a few
exceptions (increased POX and SOD activities at 0.1 mg/ml at 0.01 mg/ml SHR, respectively), suggesting that CAT is the major responsible for the elimination of reactive oxygen species generated
during the exposure of C. murale seedlings to SHR. Application of CHL A induced changes in the
activities of antioxidant enzymes, similar to those observed on SHR treatments. The treatment
with pHB A did not significantly change the activity of POX and SOD, while CAT activity was significantly increased at all applied concentrations. These data highlight the phytotoxic potential as
a highly efficient strategy of S. halepense to invade new habitats.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5569
ER  - 

@conference{
author = "Simonović, Milica and Nestorović Živković, Jasmina and Bjedov, Ivana and Nešić, Marija and Jovanović, Vladan and Giba, Zlatko and Gašić, Uroš and Mišić, Danijela and Dmitrović, Slavica",
year = "2022",
abstract = "The use of bioherbicides offers the possibility to suppress harmful weeds by ecologically acceptable methods and thus increase crop productivity. Progress in understanding the mechanisms of bioherbicides action and weed defense response mechanisms, could help in defining
novel weed control strategies. This work highlights the bioherbicide potential of rhizomes of Sorghum halepense L., characterized by a high content of phenolic acids, especially p-hydroxybenzoic
acid (pHB A) and chlorogenic acid (CHL A). Methanol extract of S. halepense rhizome (SHR), and its
major bioactive principles, displayed phytotoxic effects against Chenopodium murale L., which is a
common weed of fields and roadsides. Besides reduction in C. murale seed germination, rhizome
extract of S. halepense applied in the concentrations of 0.01, 0.1, 1 and 2 mg/ml, induced changes
in the activity of antioxidant enzymes in seedlings. Catalase (CAT) activity was increased, while
peroxidase (POX) and superoxide dismutase (SOD) activities were generally decreased, with a few
exceptions (increased POX and SOD activities at 0.1 mg/ml at 0.01 mg/ml SHR, respectively), suggesting that CAT is the major responsible for the elimination of reactive oxygen species generated
during the exposure of C. murale seedlings to SHR. Application of CHL A induced changes in the
activities of antioxidant enzymes, similar to those observed on SHR treatments. The treatment
with pHB A did not significantly change the activity of POX and SOD, while CAT activity was significantly increased at all applied concentrations. These data highlight the phytotoxic potential as
a highly efficient strategy of S. halepense to invade new habitats.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5569"
}

Simonović, M., Nestorović Živković, J., Bjedov, I., Nešić, M., Jovanović, V., Giba, Z., Gašić, U., Mišić, D.,& Dmitrović, S.. (2022). Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 80.
https://hdl.handle.net/21.15107/rcub_ibiss_5569

Simonović M, Nestorović Živković J, Bjedov I, Nešić M, Jovanović V, Giba Z, Gašić U, Mišić D, Dmitrović S. Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:80.
https://hdl.handle.net/21.15107/rcub_ibiss_5569 .

Simonović, Milica, Nestorović Živković, Jasmina, Bjedov, Ivana, Nešić, Marija, Jovanović, Vladan, Giba, Zlatko, Gašić, Uroš, Mišić, Danijela, Dmitrović, Slavica, "Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):80,
https://hdl.handle.net/21.15107/rcub_ibiss_5569 .

TY  - CONF
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Bjedov, Ivana
AU  - Nešić, Marija
AU  - Jovanović, Vladan
AU  - Gašić, Uroš
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5572
AB  - Nowadays, one of the priorities in agricultural production is the usage of natural bioherbicides
for weed control, instead of commercial herbicides which have a strong negative effect on human
health and the environment. Due to its widespread distribution and the economic losses it causes
to the global economy, Sorghum halepense has been described as one of the world’s most invasive
plant and a dangerous weed. We here examined the possibility to exploit the phytotoxic and bioherbicide potential of this species against other weed species: Amaranthus retroflexus, Chenopodium murale and Datura stramonium. The rhizome extracts of S. halepense (concentration of 0.01,
0.1, 1, 2, and 5 mg/ml) notably reduce the final seed germination of C. murale and D. stramonium,
especially at the highest investigated concentration, while A. retroflexus seed germination was
not affected. Early seedling growth of A. retroflexus was stimulated by the rhizome extract, while in
C. murale and D. stramonium the values of growth parameters were reduced. As revealed by UHPLC/
DAD/qqqMS2
 metabolic profiling of polyphenolics, major bioactive compounds in S. halepense rhizom
extract were phenolic acids: p-hydroxybensoic acid (pHB A), chlorogenic acid (CHL A), and p-coumaric acid (pCUM A). Analysis of bioherbicide potential of these compounds, applied in a series of
concentrations (0.005, 0.01, 0.1, 0.2, and 0.3 mg/ml), revealed that pHB A, CHL A, and pCUM A, are
the main responsible for the overall effects of S. halepense rhizom on selected weeds. These results
highlight Sorghum halepense (L.) rhizomes, and their main constituents, as potent bioherbicides.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Bioherbicidal evaluation of Sorghum halepense (L.) rhizome extract and its bioactive components against selected weed species
SP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5572
ER  - 

@conference{
author = "Nestorović Živković, Jasmina and Dmitrović, Slavica and Bjedov, Ivana and Nešić, Marija and Jovanović, Vladan and Gašić, Uroš and Mišić, Danijela",
year = "2022",
abstract = "Nowadays, one of the priorities in agricultural production is the usage of natural bioherbicides
for weed control, instead of commercial herbicides which have a strong negative effect on human
health and the environment. Due to its widespread distribution and the economic losses it causes
to the global economy, Sorghum halepense has been described as one of the world’s most invasive
plant and a dangerous weed. We here examined the possibility to exploit the phytotoxic and bioherbicide potential of this species against other weed species: Amaranthus retroflexus, Chenopodium murale and Datura stramonium. The rhizome extracts of S. halepense (concentration of 0.01,
0.1, 1, 2, and 5 mg/ml) notably reduce the final seed germination of C. murale and D. stramonium,
especially at the highest investigated concentration, while A. retroflexus seed germination was
not affected. Early seedling growth of A. retroflexus was stimulated by the rhizome extract, while in
C. murale and D. stramonium the values of growth parameters were reduced. As revealed by UHPLC/
DAD/qqqMS2
 metabolic profiling of polyphenolics, major bioactive compounds in S. halepense rhizom
extract were phenolic acids: p-hydroxybensoic acid (pHB A), chlorogenic acid (CHL A), and p-coumaric acid (pCUM A). Analysis of bioherbicide potential of these compounds, applied in a series of
concentrations (0.005, 0.01, 0.1, 0.2, and 0.3 mg/ml), revealed that pHB A, CHL A, and pCUM A, are
the main responsible for the overall effects of S. halepense rhizom on selected weeds. These results
highlight Sorghum halepense (L.) rhizomes, and their main constituents, as potent bioherbicides.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Bioherbicidal evaluation of Sorghum halepense (L.) rhizome extract and its bioactive components against selected weed species",
pages = "121",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5572"
}

Nestorović Živković, J., Dmitrović, S., Bjedov, I., Nešić, M., Jovanović, V., Gašić, U.,& Mišić, D.. (2022). Bioherbicidal evaluation of Sorghum halepense (L.) rhizome extract and its bioactive components against selected weed species. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 121.
https://hdl.handle.net/21.15107/rcub_ibiss_5572

Nestorović Živković J, Dmitrović S, Bjedov I, Nešić M, Jovanović V, Gašić U, Mišić D. Bioherbicidal evaluation of Sorghum halepense (L.) rhizome extract and its bioactive components against selected weed species. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:121.
https://hdl.handle.net/21.15107/rcub_ibiss_5572 .

Nestorović Živković, Jasmina, Dmitrović, Slavica, Bjedov, Ivana, Nešić, Marija, Jovanović, Vladan, Gašić, Uroš, Mišić, Danijela, "Bioherbicidal evaluation of Sorghum halepense (L.) rhizome extract and its bioactive components against selected weed species" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):121,
https://hdl.handle.net/21.15107/rcub_ibiss_5572 .

TY  - JOUR
AU  - Bjedov, Ivana
AU  - Obratov-Petković, Dragica
AU  - Rakonjac, Vera
AU  - Skočajić, Dragana
AU  - Bojović, Srđan
AU  - Marković, Milena
AU  - Dajić-Stevanović, Zora
PY  - 2021
UR  - https://journals.pan.pl/dlibra/publication/136700/edition/122183/content
UR  - https://abcbot.pl/2021_63_2-issue.php
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4860
AB  - Morpho-anatomical characteristics of Vaccinium myrtillus, V. uliginosum and V. vitis-idaea leaves from several sites of the Central Balkans were examined. The aim of this study was to investigate for the first time morpho-anatomical leaf traits of these species in the studied populations and to identify traits that follow a specific trend along the gradients of climate factors. Leaf traits that discriminate Vaccinium species were as follows: depth of the adaxial cuticule (AdC), thickness of the palisade tissue (PT), thickness of the spongy tissue (ST), height of the abaxial epidermal cells (AbE), height of the abaxial cuticule (AbC) and leaf thickness (LT). Populations of V. myrtillus were characterized by the smallest, and populations of V. vitis-idaea by the highest values for AdC, PT, ST, AbE and LT. Additionally, AbC was significantly larger for V. uliginosum in comparison to two other species. On the basis of morpho-anatomical traits, intraspecific variability of the studied species was explored by Principal Component Analysis (PCA), Cluster Analysis (CA) and Analysis of Variance (ANOVA). CA based on 10 morpho-anatomical traits showed that populations of V. myrtillus and V. uliginosum that grew at lower altitudes (characterized by higher mean annual temperature) are more similar to each other. Especially V. myrtillus was responsive to the elevational gradient and exhibited the highest plasticity in morpho-anatomical leaf traits. Populations of V. vitis-idaea had a different pattern of differentiation along the elevational gradient. CA showed that the populations at the lowest and at the highest altitudes were more similar according to the morpho-anatomical leaf traits, meaning that evergreen leaves were more resistant to environmental conditions.
PB  - Warszawa: Polish Academy of Sciences
T2  - Acta Biologica Cracoviensia Series Botanica
T1  - Nontrivial variations of morpho-anatomical leaf traits in natural South-eastern populations of vaccinium species from Central Balkans
IS  - 2
VL  - 63
DO  - 10.24425/abcsb.2021.136700
SP  - 7
EP  - 16
ER  - 

@article{
author = "Bjedov, Ivana and Obratov-Petković, Dragica and Rakonjac, Vera and Skočajić, Dragana and Bojović, Srđan and Marković, Milena and Dajić-Stevanović, Zora",
year = "2021",
abstract = "Morpho-anatomical characteristics of Vaccinium myrtillus, V. uliginosum and V. vitis-idaea leaves from several sites of the Central Balkans were examined. The aim of this study was to investigate for the first time morpho-anatomical leaf traits of these species in the studied populations and to identify traits that follow a specific trend along the gradients of climate factors. Leaf traits that discriminate Vaccinium species were as follows: depth of the adaxial cuticule (AdC), thickness of the palisade tissue (PT), thickness of the spongy tissue (ST), height of the abaxial epidermal cells (AbE), height of the abaxial cuticule (AbC) and leaf thickness (LT). Populations of V. myrtillus were characterized by the smallest, and populations of V. vitis-idaea by the highest values for AdC, PT, ST, AbE and LT. Additionally, AbC was significantly larger for V. uliginosum in comparison to two other species. On the basis of morpho-anatomical traits, intraspecific variability of the studied species was explored by Principal Component Analysis (PCA), Cluster Analysis (CA) and Analysis of Variance (ANOVA). CA based on 10 morpho-anatomical traits showed that populations of V. myrtillus and V. uliginosum that grew at lower altitudes (characterized by higher mean annual temperature) are more similar to each other. Especially V. myrtillus was responsive to the elevational gradient and exhibited the highest plasticity in morpho-anatomical leaf traits. Populations of V. vitis-idaea had a different pattern of differentiation along the elevational gradient. CA showed that the populations at the lowest and at the highest altitudes were more similar according to the morpho-anatomical leaf traits, meaning that evergreen leaves were more resistant to environmental conditions.",
publisher = "Warszawa: Polish Academy of Sciences",
journal = "Acta Biologica Cracoviensia Series Botanica",
title = "Nontrivial variations of morpho-anatomical leaf traits in natural South-eastern populations of vaccinium species from Central Balkans",
number = "2",
volume = "63",
doi = "10.24425/abcsb.2021.136700",
pages = "7-16"
}

Bjedov, I., Obratov-Petković, D., Rakonjac, V., Skočajić, D., Bojović, S., Marković, M.,& Dajić-Stevanović, Z.. (2021). Nontrivial variations of morpho-anatomical leaf traits in natural South-eastern populations of vaccinium species from Central Balkans. in Acta Biologica Cracoviensia Series Botanica
Warszawa: Polish Academy of Sciences., 63(2), 7-16.
https://doi.org/10.24425/abcsb.2021.136700

Bjedov I, Obratov-Petković D, Rakonjac V, Skočajić D, Bojović S, Marković M, Dajić-Stevanović Z. Nontrivial variations of morpho-anatomical leaf traits in natural South-eastern populations of vaccinium species from Central Balkans. in Acta Biologica Cracoviensia Series Botanica. 2021;63(2):7-16.
doi:10.24425/abcsb.2021.136700 .

Bjedov, Ivana, Obratov-Petković, Dragica, Rakonjac, Vera, Skočajić, Dragana, Bojović, Srđan, Marković, Milena, Dajić-Stevanović, Zora, "Nontrivial variations of morpho-anatomical leaf traits in natural South-eastern populations of vaccinium species from Central Balkans" in Acta Biologica Cracoviensia Series Botanica, 63, no. 2 (2021):7-16,
https://doi.org/10.24425/abcsb.2021.136700 . .

TY  - JOUR
AU  - Bjedov, Ivana
AU  - Obratov-Petkovic, Dragica
AU  - Misic, Danijela
AU  - Šiler, Branislav
AU  - Aleksic, Jelena M.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2050
AB  - Vaccinium myrtillus L., Vaccinium uliginosum L. and Vaccinium
   vitis-idaea L. are perennial, cold-adapted clonal shrubs distributed
   throughout Europe, northern Asia and North America. Due to their usage
   in food (berries) and pharmaceutical industry (berries and leaves),
   their natural populations are exposed to anthropogenic and other impacts
   that affect their genetic make-up. We analyzed 14 fragmentary
   distributed and small-sized peripheral populations of these species from
   the Balkans, which represents the southeastern-European marginal area of
   their wide European distributions, using RAPD molecular markers. The
   contemporary genetic patterns in all three species within the Balkans
   were generally similar, and in comparison to previous reports on
   populations of these species found in northward Europe, where they have
   a more continuous distribution, the levels of genetic diversity were
   more or less halved, genetic differentiation was several times higher,
   gene flow exceptionally low, and the expected prevalence of clonal
   individuals was lacking. The population dynamics of all three species
   within the Balkans was complex and distinct, and was characterized by a
   past admixture of individuals from discrete populations of the same
   species and interspecific hybridisation not only between V. myrtillus
   and V. vitis-idaea but also between V. uliginosum and V. vitis-idaea,
   the latter not being reported to date. Conservation measures suitable
   for preservation of presumably genetically distinct portions of the
   Balkans' gene pools of studied species have been suggested, while the
   utility of interspecific hybrids in breeding programs and/or in
   food/pharmaceutical industry is yet to be assessed.
T2  - Silva Fennica
T1  - Genetic patterns in range-edge populations of Vaccinium species from the
 central Balkans: implications on conservation prospects and sustainable
 usage
IS  - 1283
VL  - 49
DO  - 10.14214/sf.1283
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2050
ER  - 

@article{
author = "Bjedov, Ivana and Obratov-Petkovic, Dragica and Misic, Danijela and Šiler, Branislav and Aleksic, Jelena M.",
year = "2015",
abstract = "Vaccinium myrtillus L., Vaccinium uliginosum L. and Vaccinium
   vitis-idaea L. are perennial, cold-adapted clonal shrubs distributed
   throughout Europe, northern Asia and North America. Due to their usage
   in food (berries) and pharmaceutical industry (berries and leaves),
   their natural populations are exposed to anthropogenic and other impacts
   that affect their genetic make-up. We analyzed 14 fragmentary
   distributed and small-sized peripheral populations of these species from
   the Balkans, which represents the southeastern-European marginal area of
   their wide European distributions, using RAPD molecular markers. The
   contemporary genetic patterns in all three species within the Balkans
   were generally similar, and in comparison to previous reports on
   populations of these species found in northward Europe, where they have
   a more continuous distribution, the levels of genetic diversity were
   more or less halved, genetic differentiation was several times higher,
   gene flow exceptionally low, and the expected prevalence of clonal
   individuals was lacking. The population dynamics of all three species
   within the Balkans was complex and distinct, and was characterized by a
   past admixture of individuals from discrete populations of the same
   species and interspecific hybridisation not only between V. myrtillus
   and V. vitis-idaea but also between V. uliginosum and V. vitis-idaea,
   the latter not being reported to date. Conservation measures suitable
   for preservation of presumably genetically distinct portions of the
   Balkans' gene pools of studied species have been suggested, while the
   utility of interspecific hybrids in breeding programs and/or in
   food/pharmaceutical industry is yet to be assessed.",
journal = "Silva Fennica",
title = "Genetic patterns in range-edge populations of Vaccinium species from the
 central Balkans: implications on conservation prospects and sustainable
 usage",
number = "1283",
volume = "49",
doi = "10.14214/sf.1283",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2050"
}

Bjedov, I., Obratov-Petkovic, D., Misic, D., Šiler, B.,& Aleksic, J. M.. (2015). Genetic patterns in range-edge populations of Vaccinium species from the
 central Balkans: implications on conservation prospects and sustainable
 usage. in Silva Fennica, 49(1283).
https://doi.org/10.14214/sf.1283
https://hdl.handle.net/21.15107/rcub_ibiss_2050

Bjedov I, Obratov-Petkovic D, Misic D, Šiler B, Aleksic JM. Genetic patterns in range-edge populations of Vaccinium species from the
 central Balkans: implications on conservation prospects and sustainable
 usage. in Silva Fennica. 2015;49(1283).
doi:10.14214/sf.1283
https://hdl.handle.net/21.15107/rcub_ibiss_2050 .

Bjedov, Ivana, Obratov-Petkovic, Dragica, Misic, Danijela, Šiler, Branislav, Aleksic, Jelena M., "Genetic patterns in range-edge populations of Vaccinium species from the
 central Balkans: implications on conservation prospects and sustainable
 usage" in Silva Fennica, 49, no. 1283 (2015),
https://doi.org/10.14214/sf.1283 .,
https://hdl.handle.net/21.15107/rcub_ibiss_2050 .