@article{
author = "Petrovic, Jelena and Lazic, Katarina and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2014",
abstract = "The aim of this study was to demonstrate that two REM clusters, which
emerge following bilateral pedunculopontine tegmental nucleus (PPT)
lesions in rats, are two functionally distinct REM states.
We performed the experiments in Wistar rats, chronically instrumented
for sleep recording. Bilateral PPT lesions were produced by the
microinfusion of 100 nl of 0.1 M ibotenic acid (IBO). Following a
recovery period of 2 weeks, we recorded their sleep for 6 h. Bilateral
PPT lesions were identified by NADPH diaphorase histochemistry.
We applied Fourier analysis to the signals acquired throughout the 6 h
recordings, and each 10 s epoch was differentiated as a Wake, NREM or
REM state. We analyzed the topography of the sleep/wake states
architecture and their transition structure, their all state-related EEG
microstructures, and the sensorimotor (SMCx) and motor (MCx) cortex REM
related cortico-muscular coherences (CMCs).
Bilateral PPT lesion in rats increased the likelihood of the emergence
of two distinct REM sleep states, specifically expressed within the MCx:
REM1 and REM2. Bilateral PPT lesion did not change the sleep/wake states
architecture of the SMCx, but pathologically increased the duration of
REM1 within the MCx, alongside increasing Wake/REM1/Wake and
NREM/REM2/NREM transitions within both cortices. In addition, the
augmented total REM SMCx EEG beta amplitude and REM1 MCx EEG theta
amplitude was the underlying EEG microstructure pathology.
PPT lesion induced REM1 and REM2 are differential states with regard to
total EMG power, topographically distinct EEG microstructures, and
locomotor drives to nuchal musculature. (C) 2014 Elsevier B.V. All
rights reserved.",
journal = "Behavioural Brain Research",
title = "REM sleep diversity following the pedunculopontine tegmental nucleus
lesion in rat",
volume = "271",
doi = "10.1016/j.bbr.2014.06.026",
pages = "258-268"
}
