TY  - CONF
AU  - Brkljačić, Jelena
AU  - Jovanović, Mirna
AU  - Teofilović, Ana
AU  - Mićić, Bojana
AU  - Vratarić, Miloš
AU  - Milosavljević, Dragica
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Milivojević, Jasminka
AU  - Đorđević, Ana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6706
AB  - Healthy dietary habits with abundant fruit consumption may reduce prevalence and positively affect 
development and progression of various chronic diseases including obesity and insulin-resistance related 
diseases. Strawberry (Fragaria × ananassa Duch.) represent a valuable source of vitamins, minerals, fatty 
acids, dietary fiber, and various bioactive polyphenolic compounds such as anthocyanins and flavonoids 
which are known for their antioxidant and anti-inflammatory activities. In this study we evaluated the 
effect of newly introduced strawberry cultivar „Aprika” supplemented in the form of lyophilized powder, 
on obesity-related metabolic alterations in high-fat-diet fed mice. We hypothesized that chronic (8 weeks) 
supplementation of lyophilized strawberries in the amount comparable to two servings per day in humans, 
would  ameliorate  insulin  resistance  associated  with  obesity  in  C57BL/6  mice.  Furthermore,  we 
hypothesized that strawberry meal consumed at 7PM i.e. before active feeding period of mice (which 
corresponds to early breakfast in humans) would induce more pronounced beneficial effects compared to 
meal consumed at 7AM i.e. at the end of the active feeding period (which corresponds to late dinner in 
humans). High fat diet induced hyperglycaemia, hyperinsulinaemia, insulin resistance and obesity; and 
disturbed hepatic insulin signaling. Lyophilized strawberries, only when consumed before feeding period, 
reduced body weight gain and improved insulin sensitivity induced by high-fat diet (evidenced by lower 
area under the curve after an intraperitoneal GTT, reduced serum insulin levels and an insulin resistance 
index (IR-HOMA). Strawberry meals consumed after active feeding period had no such effect. However, 
strawberries, regardless of the time of consumption, restored protein level of Insulin receptor substrate 1 
(IRS1) in the liver and increased hepatic antioxidant enzymes level. In conclusion, strawberries improve 
insulin  sensitivity  and  raise  hepatic  antioxidant  capacity  in  mouse  model  of  diet  induced  obesity. 
Nevertheless, more beneficial effects were achieved when strawberries were consumed before active 
feeding period, as an early breakfast.
PB  - Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina
T1  - Dietary supplementation with liophilised strawberry improves insulin  sensitivity and redox status in mouse model of diet induced obesity
SP  - 38
ER  - 

@conference{
author = "Brkljačić, Jelena and Jovanović, Mirna and Teofilović, Ana and Mićić, Bojana and Vratarić, Miloš and Milosavljević, Dragica and Dragišić Maksimović, Jelena and Maksimović, Vuk and Milivojević, Jasminka and Đorđević, Ana",
year = "2024",
abstract = "Healthy dietary habits with abundant fruit consumption may reduce prevalence and positively affect 
development and progression of various chronic diseases including obesity and insulin-resistance related 
diseases. Strawberry (Fragaria × ananassa Duch.) represent a valuable source of vitamins, minerals, fatty 
acids, dietary fiber, and various bioactive polyphenolic compounds such as anthocyanins and flavonoids 
which are known for their antioxidant and anti-inflammatory activities. In this study we evaluated the 
effect of newly introduced strawberry cultivar „Aprika” supplemented in the form of lyophilized powder, 
on obesity-related metabolic alterations in high-fat-diet fed mice. We hypothesized that chronic (8 weeks) 
supplementation of lyophilized strawberries in the amount comparable to two servings per day in humans, 
would  ameliorate  insulin  resistance  associated  with  obesity  in  C57BL/6  mice.  Furthermore,  we 
hypothesized that strawberry meal consumed at 7PM i.e. before active feeding period of mice (which 
corresponds to early breakfast in humans) would induce more pronounced beneficial effects compared to 
meal consumed at 7AM i.e. at the end of the active feeding period (which corresponds to late dinner in 
humans). High fat diet induced hyperglycaemia, hyperinsulinaemia, insulin resistance and obesity; and 
disturbed hepatic insulin signaling. Lyophilized strawberries, only when consumed before feeding period, 
reduced body weight gain and improved insulin sensitivity induced by high-fat diet (evidenced by lower 
area under the curve after an intraperitoneal GTT, reduced serum insulin levels and an insulin resistance 
index (IR-HOMA). Strawberry meals consumed after active feeding period had no such effect. However, 
strawberries, regardless of the time of consumption, restored protein level of Insulin receptor substrate 1 
(IRS1) in the liver and increased hepatic antioxidant enzymes level. In conclusion, strawberries improve 
insulin  sensitivity  and  raise  hepatic  antioxidant  capacity  in  mouse  model  of  diet  induced  obesity. 
Nevertheless, more beneficial effects were achieved when strawberries were consumed before active 
feeding period, as an early breakfast.",
publisher = "Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina",
title = "Dietary supplementation with liophilised strawberry improves insulin  sensitivity and redox status in mouse model of diet induced obesity",
pages = "38"
}

Brkljačić, J., Jovanović, M., Teofilović, A., Mićić, B., Vratarić, M., Milosavljević, D., Dragišić Maksimović, J., Maksimović, V., Milivojević, J.,& Đorđević, A.. (2024). Dietary supplementation with liophilised strawberry improves insulin  sensitivity and redox status in mouse model of diet induced obesity. in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina
Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 38.

Brkljačić J, Jovanović M, Teofilović A, Mićić B, Vratarić M, Milosavljević D, Dragišić Maksimović J, Maksimović V, Milivojević J, Đorđević A. Dietary supplementation with liophilised strawberry improves insulin  sensitivity and redox status in mouse model of diet induced obesity. in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina. 2024;:38..

Brkljačić, Jelena, Jovanović, Mirna, Teofilović, Ana, Mićić, Bojana, Vratarić, Miloš, Milosavljević, Dragica, Dragišić Maksimović, Jelena, Maksimović, Vuk, Milivojević, Jasminka, Đorđević, Ana, "Dietary supplementation with liophilised strawberry improves insulin  sensitivity and redox status in mouse model of diet induced obesity" in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina (2024):38.

TY  - CONF
AU  - Jovanović, Mirna
AU  - Milosavljević, Dragica
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Milivojević, Jasminka
AU  - Đorđević, Ana
AU  - Brkljačić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6238
AB  - Insulin resistance is a state where a normal amount of insulin can’t provoke an appropriate metabolic response. Insulin promotes membrane trafficking of the glucose transporter GLUT4 from the storage vesicles to the plasma membrane in white adipose tissue. Adipocytes use glucose for lipogenesis and store the energy as lipid droplets. If adipocytes are unable to uptake glucose, a chronic state of hyperglycemia is developed, with severe health consequences. Polyphenols are natural anti-inflammatory and antioxidant agents. Food rich in polyphenols has been suggested to exert an ameliorative effect on restoring insulin sensitivity, with the main identified target being AMPK1,2, one of the key sensors of intracellular energy. Here, we tested the effect of methanol extracts from three newly introduced strawberry (Fragaria x ananassa, Duch.) cultivars – 'Aprika', 'Sandra' and 'Quicky' on glucose metabolism in 3T3-F442A adipocytes. It was determined that 'Aprika' has the highest total phenolic content, relative to the other two cultivars. After 72-h exposure, none of the strawberry cultivars affected adipocyte cell growth significantly. Protein expression analysis of the differentiated adipocytes suggested 'Aprika', but not the other two cultivars, significantly increased the AMPK expression, as well as GLUT4, thus increasing glucose uptake. Strawberry extracts did not significantly affect the differentiation of adipocytes (SIRT1 and PPARγ), nor the fatty acid synthesis (ACC). Conclusively, the 'Aprika' methanol extract with high phenolic content exerts an ameliorative effect on glucose uptake, presumably through activation of the AMPK-dependent mechanism of GLUT4 trafficking. The systemic effects of the ‘Aprika’ cultivar should be further investigated. Implications of the research are decreased hyperglycemia in obese and diabetic patients, by the introduction of the 'Aprika' strawberry cultivar into everyday diet. References 1. Zhao L, Zou T, Gomez NA et al. Raspberry alleviates obesity-induced inflammation and insulin resistance in skeletal muscle through activation of AMP-activated protein kinase (AMPK) α1. Nutr & Diabetes, 2018, 8, 39. 2. Xu W, Luo Y, Yin J, Luo F. Targeting AMPK signaling by polyphenols: A novel strategy for tackling aging. Food & Function. 2023.
PB  - Belgrade: Faculty of Chemistry
C3  - Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
T1  - Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes
SP  - 131
EP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6238
ER  - 

@conference{
author = "Jovanović, Mirna and Milosavljević, Dragica and Dragišić Maksimović, Jelena and Maksimović, Vuk and Milivojević, Jasminka and Đorđević, Ana and Brkljačić, Jelena",
year = "2023",
abstract = "Insulin resistance is a state where a normal amount of insulin can’t provoke an appropriate metabolic response. Insulin promotes membrane trafficking of the glucose transporter GLUT4 from the storage vesicles to the plasma membrane in white adipose tissue. Adipocytes use glucose for lipogenesis and store the energy as lipid droplets. If adipocytes are unable to uptake glucose, a chronic state of hyperglycemia is developed, with severe health consequences. Polyphenols are natural anti-inflammatory and antioxidant agents. Food rich in polyphenols has been suggested to exert an ameliorative effect on restoring insulin sensitivity, with the main identified target being AMPK1,2, one of the key sensors of intracellular energy. Here, we tested the effect of methanol extracts from three newly introduced strawberry (Fragaria x ananassa, Duch.) cultivars – 'Aprika', 'Sandra' and 'Quicky' on glucose metabolism in 3T3-F442A adipocytes. It was determined that 'Aprika' has the highest total phenolic content, relative to the other two cultivars. After 72-h exposure, none of the strawberry cultivars affected adipocyte cell growth significantly. Protein expression analysis of the differentiated adipocytes suggested 'Aprika', but not the other two cultivars, significantly increased the AMPK expression, as well as GLUT4, thus increasing glucose uptake. Strawberry extracts did not significantly affect the differentiation of adipocytes (SIRT1 and PPARγ), nor the fatty acid synthesis (ACC). Conclusively, the 'Aprika' methanol extract with high phenolic content exerts an ameliorative effect on glucose uptake, presumably through activation of the AMPK-dependent mechanism of GLUT4 trafficking. The systemic effects of the ‘Aprika’ cultivar should be further investigated. Implications of the research are decreased hyperglycemia in obese and diabetic patients, by the introduction of the 'Aprika' strawberry cultivar into everyday diet. References 1. Zhao L, Zou T, Gomez NA et al. Raspberry alleviates obesity-induced inflammation and insulin resistance in skeletal muscle through activation of AMP-activated protein kinase (AMPK) α1. Nutr & Diabetes, 2018, 8, 39. 2. Xu W, Luo Y, Yin J, Luo F. Targeting AMPK signaling by polyphenols: A novel strategy for tackling aging. Food & Function. 2023.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia",
title = "Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes",
pages = "131-132",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6238"
}

Jovanović, M., Milosavljević, D., Dragišić Maksimović, J., Maksimović, V., Milivojević, J., Đorđević, A.,& Brkljačić, J.. (2023). Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
Belgrade: Faculty of Chemistry., 131-132.
https://hdl.handle.net/21.15107/rcub_ibiss_6238

Jovanović M, Milosavljević D, Dragišić Maksimović J, Maksimović V, Milivojević J, Đorđević A, Brkljačić J. Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia. 2023;:131-132.
https://hdl.handle.net/21.15107/rcub_ibiss_6238 .

Jovanović, Mirna, Milosavljević, Dragica, Dragišić Maksimović, Jelena, Maksimović, Vuk, Milivojević, Jasminka, Đorđević, Ana, Brkljačić, Jelena, "Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes" in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia (2023):131-132,
https://hdl.handle.net/21.15107/rcub_ibiss_6238 .