@conference{
author = "Ademović, Nejla and Tomić, Tijana and Tanić, Nasta and Milić, Marina and Rakić, Miodrag and Tanić, Nikola",
year = "2023",
abstract = "Introduction. Glioblastoma and Astrocytoma are diffuse malignant brain tumors and characterized as the most aggressive and invasive brain cancers. Glioblastoma IDH wild-type is a primary brain tumour that develops de novo, and Astrocytoma IDH mutant is a secondary tumour which arises by progression from lower tumour grades. They are characterized by poor survival, resistance to therapy and poor prognosis which develops as a consequence of genomic instability. Genomic instability also contributes to tumour heterogeneity and provides the genomic diversity necessary for selection.
Materials and methods. 31 patients with Glioblastoma IDH wild-type and Astrocytoma IDH mutant, grade 3 and 4, were analysed for the presence of genomic instability using AP-PCR, DNA profiling method. Comparing DNA profiles between tumour tissue and normal tissue (blood) of the same patient, we detected qualitative and quantitative changes. Qualitative changes are detected as the presence and absence of bands and are the manifestation of microsatellite instability (MIN). Quantitative changes are the representation of chromosomal instability (CIN) and are detected as differences in the intensity of bands. Survival analyses were performed using Kaplan & Maier test for survival data in relation to different histological tumour type and genomic instability. Statistical differences were considered significant for p≤ 0,05.
Results. Patients with Glioblastoma IDH wild-type have significantly shorter survival compared to other histological types (p=0,025). For each histological type that we analysed and each type of instability, MIN, CIN and total genomic instability, two groups of patients were made - those with high and low instability. Patients with Glioblastoma IDH wild-type that have low total genomic instability have significantly shorter survival (p=0,045) compared to other analysed types of brain cancer. Patients with Astrocytoma IDH mutant grade 4 who have high total genomic instability and high CIN have significantly shorter survival (p=0,018, p=0,007 respectfully).
Conclusion. Patients with Glioblastoma IDH wild-type have shorter survival which makes this tumour the most aggressive and malignat of all analysed tumours. Our results show that low genomic instability in Glioblastoma IDH wild-type and high genomic instability lead by high CIN in Astrocytoma IDH mutant, gradus 4 contribute to shorter survival, which makes genomic instability a potential good prognostic marker.",
publisher = "Belgrade, Serbia: Serbian Associaton for Cancer Research",
journal = "Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia",
title = "Genomic instability as a prognostic marker in malignant brain cancer",
pages = "90-91",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6234"
}
