TY  - JOUR
AU  - Jovanovic Krivokuca, M.
AU  - Stefanoska, I.
AU  - Abu Rabi, T.
AU  - Al-Abed, Y.
AU  - Stošić-Grujičić, Stanislava
AU  - Vicovac, Lj
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2009
AB  - Introduction: Macrophage migration inhibitory factor (MIF) is expressed
   by villous and extravillous cytotrophoblast. This study was aimed to
   investigate functional relevance of MIF for human trophoblast.
   Methods: MIF mRNA and protein were documented in cytotrophoblast (CT)
   and extravillous trophoblast cell line HTR-8/SVneo by RT-PCR, Western
   blot (WB), and immunocytochemistry. Recombinant human MIF (rhMIF), or
   its specific inhibitor (S,R)-3-(4-hydroxypheny1)-4,5-dihydro-5-isoxazole
   acetic acid methyl ester (ISO-1) were used in Wound healing migration
   and Matrigel invasion tests. Potential effectors, integrin subunits and
   matrix metalloproteinases (MMP) were studied using WB and gelatin
   zymography, respectively.
   Results: Blocking endogenous MIF by ISO-1 decreased HTR-8/SVneo cell
   migration dose dependently, most significantly with 200 mu g/ml to 65\%
   of control. Supplementation with rhMIF induced a significant stimulation
   to 129\% of control with 200 ng/ml. In CT cell invasion test, ISO-1 at
   200 mu g/ml reduced invasion to 59\% of control, while rhMIF (200 ng/m1)
   induced stimulation to 159\% of control. In HTR-8/SVneo cells, invasion
   was significantly inhibited by ISO-1 to 40\%, and increased to 150\% of
   control by rhMIF (200 ng/ml). Integrin alpha 1 was reduced by ISO-1 in
   both cell types, while integrins alpha 5 and beta 1 were not changed.
   Addition of rhMIF increased integrin alpha 1. In the presence of ISO-1,
   levels of MMP-2 and MMP-9 were reduced in CT and HTR-8/SVneo, while
   rhMIF stimulated MMP-2 in CT and MMP-9 in HTR-8/SVneo cells.
   Conclusion: Reported findings provide the first insight into the
   cellular effects of MIF in human trophoblast, which acts to promote cell
   migration and invasion. (C) 2014 Elsevier Ltd. All rights reserved.
T2  - Placenta
T1  - Pharmacological inhibition of MIF interferes with trophoblast cell
 migration and invasiveness
IS  - 2
VL  - 36
DO  - 10.1016/j.placenta.2014.12.003
SP  - 150
EP  - 159
ER  - 

@article{
author = "Jovanovic Krivokuca, M. and Stefanoska, I. and Abu Rabi, T. and Al-Abed, Y. and Stošić-Grujičić, Stanislava and Vicovac, Lj",
year = "2015",
abstract = "Introduction: Macrophage migration inhibitory factor (MIF) is expressed
   by villous and extravillous cytotrophoblast. This study was aimed to
   investigate functional relevance of MIF for human trophoblast.
   Methods: MIF mRNA and protein were documented in cytotrophoblast (CT)
   and extravillous trophoblast cell line HTR-8/SVneo by RT-PCR, Western
   blot (WB), and immunocytochemistry. Recombinant human MIF (rhMIF), or
   its specific inhibitor (S,R)-3-(4-hydroxypheny1)-4,5-dihydro-5-isoxazole
   acetic acid methyl ester (ISO-1) were used in Wound healing migration
   and Matrigel invasion tests. Potential effectors, integrin subunits and
   matrix metalloproteinases (MMP) were studied using WB and gelatin
   zymography, respectively.
   Results: Blocking endogenous MIF by ISO-1 decreased HTR-8/SVneo cell
   migration dose dependently, most significantly with 200 mu g/ml to 65\%
   of control. Supplementation with rhMIF induced a significant stimulation
   to 129\% of control with 200 ng/ml. In CT cell invasion test, ISO-1 at
   200 mu g/ml reduced invasion to 59\% of control, while rhMIF (200 ng/m1)
   induced stimulation to 159\% of control. In HTR-8/SVneo cells, invasion
   was significantly inhibited by ISO-1 to 40\%, and increased to 150\% of
   control by rhMIF (200 ng/ml). Integrin alpha 1 was reduced by ISO-1 in
   both cell types, while integrins alpha 5 and beta 1 were not changed.
   Addition of rhMIF increased integrin alpha 1. In the presence of ISO-1,
   levels of MMP-2 and MMP-9 were reduced in CT and HTR-8/SVneo, while
   rhMIF stimulated MMP-2 in CT and MMP-9 in HTR-8/SVneo cells.
   Conclusion: Reported findings provide the first insight into the
   cellular effects of MIF in human trophoblast, which acts to promote cell
   migration and invasion. (C) 2014 Elsevier Ltd. All rights reserved.",
journal = "Placenta",
title = "Pharmacological inhibition of MIF interferes with trophoblast cell
 migration and invasiveness",
number = "2",
volume = "36",
doi = "10.1016/j.placenta.2014.12.003",
pages = "150-159"
}

Jovanovic Krivokuca, M., Stefanoska, I., Abu Rabi, T., Al-Abed, Y., Stošić-Grujičić, S.,& Vicovac, L.. (2015). Pharmacological inhibition of MIF interferes with trophoblast cell
 migration and invasiveness. in Placenta, 36(2), 150-159.
https://doi.org/10.1016/j.placenta.2014.12.003

Jovanovic Krivokuca M, Stefanoska I, Abu Rabi T, Al-Abed Y, Stošić-Grujičić S, Vicovac L. Pharmacological inhibition of MIF interferes with trophoblast cell
 migration and invasiveness. in Placenta. 2015;36(2):150-159.
doi:10.1016/j.placenta.2014.12.003 .

Jovanovic Krivokuca, M., Stefanoska, I., Abu Rabi, T., Al-Abed, Y., Stošić-Grujičić, Stanislava, Vicovac, Lj, "Pharmacological inhibition of MIF interferes with trophoblast cell
 migration and invasiveness" in Placenta, 36, no. 2 (2015):150-159,
https://doi.org/10.1016/j.placenta.2014.12.003 . .

TY  - JOUR
AU  - Stojanović, Ivana D.
AU  - Maksimović-Ivanić, Danijela
AU  - Al-Abed, Y.
AU  - Nicoletti, F.
AU  - Stošić-Grujičić, Stanislava
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/198
AB  - We recently showed that attenuation of inflammatory cytokine MIF with pharmacological inhibitor ISO-1 down-regulates the immune-mediated diabetes in mice. Here we explore the effects of MIF neutralization by ISO-1 on the local inflammatory pathway of the disease. In vivo treatment of mice with ISO-1 inhibited the expression of pro-inflammatory cytokines and iNOS in the pancreatic islets. Moreover, ISO-1 affected in vitro cytokine-induced NO pro­duction by fibroblasts, endothelial cells, insulinoma cells, and pancreatic islets, and rescued β cells from NO-dependent damage. These results suggest regulatory potential of ISO-1 at the level of the pancreas which can preserve the target tissue from autoimmune attack.
AB  - Nedavno smo pokazali da neutralizacija inflamatornog citokina MIF-a farmakološkim inhibitorom ISO-1 negativno reguliše imunski posredovan dijabetes miševa. U ovom radu ispitivali smo efekte neutralizacije MIF-a pomoću ISO-1 na lokalne inflamatorne puteve bolesti. In vivo tretman miševa pomoću ISO-1 je inhibirao ekspresiju proinflamatornih citokina i inducibilne sintaze azot monoksida u ostrvcima pankreasa. Štaviše, ISO-1 je remetio in vitro produkciju azot monoksida indukovan u citokinima u fibroblastima, ćelijama endotela, insulinomama i pankreasnih ostrvaca i tako sačuvao beta ćelije od oštećenja izazvanih azot monoksidom. Ovi rezultati ukazuju na regulatorni potencijal ISO-1 na nivou ciljnog tkiva koji štiti ciljno tkivo od autoimunog ataka.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Kontrola finalnog stadijuma imunski posredovanog dijabetesa pomoću antagonista faktora inhibicije migracije makrofaga (S,R)-3-(4-hidroksifenil)-4,5-dihidro-5-izoksazol metil estra sirćetne kiseline
T1  - Control of the of the final stage of immune-mediated diabetes by ISO-1, an antagonist of macrophage migration inhibitory factor
IS  - 3
VL  - 60
DO  - 10.2298/ABS0803389S
SP  - 389
EP  - 401
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_198
ER  - 

@article{
author = "Stojanović, Ivana D. and Maksimović-Ivanić, Danijela and Al-Abed, Y. and Nicoletti, F. and Stošić-Grujičić, Stanislava",
year = "2008, 2008",
abstract = "We recently showed that attenuation of inflammatory cytokine MIF with pharmacological inhibitor ISO-1 down-regulates the immune-mediated diabetes in mice. Here we explore the effects of MIF neutralization by ISO-1 on the local inflammatory pathway of the disease. In vivo treatment of mice with ISO-1 inhibited the expression of pro-inflammatory cytokines and iNOS in the pancreatic islets. Moreover, ISO-1 affected in vitro cytokine-induced NO pro­duction by fibroblasts, endothelial cells, insulinoma cells, and pancreatic islets, and rescued β cells from NO-dependent damage. These results suggest regulatory potential of ISO-1 at the level of the pancreas which can preserve the target tissue from autoimmune attack., Nedavno smo pokazali da neutralizacija inflamatornog citokina MIF-a farmakološkim inhibitorom ISO-1 negativno reguliše imunski posredovan dijabetes miševa. U ovom radu ispitivali smo efekte neutralizacije MIF-a pomoću ISO-1 na lokalne inflamatorne puteve bolesti. In vivo tretman miševa pomoću ISO-1 je inhibirao ekspresiju proinflamatornih citokina i inducibilne sintaze azot monoksida u ostrvcima pankreasa. Štaviše, ISO-1 je remetio in vitro produkciju azot monoksida indukovan u citokinima u fibroblastima, ćelijama endotela, insulinomama i pankreasnih ostrvaca i tako sačuvao beta ćelije od oštećenja izazvanih azot monoksidom. Ovi rezultati ukazuju na regulatorni potencijal ISO-1 na nivou ciljnog tkiva koji štiti ciljno tkivo od autoimunog ataka.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Kontrola finalnog stadijuma imunski posredovanog dijabetesa pomoću antagonista faktora inhibicije migracije makrofaga (S,R)-3-(4-hidroksifenil)-4,5-dihidro-5-izoksazol metil estra sirćetne kiseline, Control of the of the final stage of immune-mediated diabetes by ISO-1, an antagonist of macrophage migration inhibitory factor",
number = "3",
volume = "60",
doi = "10.2298/ABS0803389S",
pages = "389-401",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_198"
}

Stojanović, I. D., Maksimović-Ivanić, D., Al-Abed, Y., Nicoletti, F.,& Stošić-Grujičić, S.. (2008). Kontrola finalnog stadijuma imunski posredovanog dijabetesa pomoću antagonista faktora inhibicije migracije makrofaga (S,R)-3-(4-hidroksifenil)-4,5-dihidro-5-izoksazol metil estra sirćetne kiseline. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 60(3), 389-401.
https://doi.org/10.2298/ABS0803389S
https://hdl.handle.net/21.15107/rcub_ibiss_198

Stojanović ID, Maksimović-Ivanić D, Al-Abed Y, Nicoletti F, Stošić-Grujičić S. Kontrola finalnog stadijuma imunski posredovanog dijabetesa pomoću antagonista faktora inhibicije migracije makrofaga (S,R)-3-(4-hidroksifenil)-4,5-dihidro-5-izoksazol metil estra sirćetne kiseline. in Archives of Biological Sciences. 2008;60(3):389-401.
doi:10.2298/ABS0803389S
https://hdl.handle.net/21.15107/rcub_ibiss_198 .

Stojanović, Ivana D., Maksimović-Ivanić, Danijela, Al-Abed, Y., Nicoletti, F., Stošić-Grujičić, Stanislava, "Kontrola finalnog stadijuma imunski posredovanog dijabetesa pomoću antagonista faktora inhibicije migracije makrofaga (S,R)-3-(4-hidroksifenil)-4,5-dihidro-5-izoksazol metil estra sirćetne kiseline" in Archives of Biological Sciences, 60, no. 3 (2008):389-401,
https://doi.org/10.2298/ABS0803389S .,
https://hdl.handle.net/21.15107/rcub_ibiss_198 .