TY  - JOUR
AU  - Vranković, Jelena
AU  - Borković Mitić, Slavica
AU  - Ilić, Bojan
AU  - Radulović, Milanka
AU  - Milošević, Slaviša
AU  - Makarov, Slobodan
AU  - Mitić, Bojan
PY  - 2017
UR  - http://scholarcommons.usf.edu/ijs/vol46/iss1/12/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2606
AB  - The concentration of 10 metallic trace elements or MTE (Cu, Fe, Zn, Mn, As, Hg, Pb, Cd, Ni, and Cr) was measured in specimens of the troglophilic millipede Apfelbeckia insculpta (L. Koch, 1867) and sediment of the cave Hadži-Prodanova Pećina in western Serbia. Some MTE, like Fe and Mn, displayed much higher concentrations compared to other elements, both in the sediment and in the body of A. insculpta. On the other hand, estimation of the bioaccumulation factor (BAF) in both males and females of A. insculpta showed values greater than 1 for xenobiotic elements compared to those that are essential. In addition to chemical analyses, we examined the activities of antioxidant enzymes (SOD, CAT, GPX, and GR) and the phase II biotransformation enzyme GST, as well as the content of –SH groups, in the body of A. insculpta. Activities of two (GR and GST) out of the five tested enzymes showed significant differences between the sexes. These results represent the first comprehensive report of antioxidant enzymes in myriapods. The noted differences in the investigated MTE and enzyme activities between the sexes of A. insculpta most likely reflect different metabolic activities and responses to environmental conditions in males and females.
T2  - International Journal of Speleology
T1  - Bioaccumulation of metallic trace elements and antioxidant enzyme activities in Apfelbeckia insculpta (L. Koch, 1867) (Diplopoda: Callipodida) from the cave Hadži-Prodanova Pećina (Serbia)
IS  - 1
VL  - 46
DO  - 10.5038/1827-806X.46.1.1981
SP  - 99
EP  - 108
ER  - 

@article{
author = "Vranković, Jelena and Borković Mitić, Slavica and Ilić, Bojan and Radulović, Milanka and Milošević, Slaviša and Makarov, Slobodan and Mitić, Bojan",
year = "2017",
abstract = "The concentration of 10 metallic trace elements or MTE (Cu, Fe, Zn, Mn, As, Hg, Pb, Cd, Ni, and Cr) was measured in specimens of the troglophilic millipede Apfelbeckia insculpta (L. Koch, 1867) and sediment of the cave Hadži-Prodanova Pećina in western Serbia. Some MTE, like Fe and Mn, displayed much higher concentrations compared to other elements, both in the sediment and in the body of A. insculpta. On the other hand, estimation of the bioaccumulation factor (BAF) in both males and females of A. insculpta showed values greater than 1 for xenobiotic elements compared to those that are essential. In addition to chemical analyses, we examined the activities of antioxidant enzymes (SOD, CAT, GPX, and GR) and the phase II biotransformation enzyme GST, as well as the content of –SH groups, in the body of A. insculpta. Activities of two (GR and GST) out of the five tested enzymes showed significant differences between the sexes. These results represent the first comprehensive report of antioxidant enzymes in myriapods. The noted differences in the investigated MTE and enzyme activities between the sexes of A. insculpta most likely reflect different metabolic activities and responses to environmental conditions in males and females.",
journal = "International Journal of Speleology",
title = "Bioaccumulation of metallic trace elements and antioxidant enzyme activities in Apfelbeckia insculpta (L. Koch, 1867) (Diplopoda: Callipodida) from the cave Hadži-Prodanova Pećina (Serbia)",
number = "1",
volume = "46",
doi = "10.5038/1827-806X.46.1.1981",
pages = "99-108"
}

Vranković, J., Borković Mitić, S., Ilić, B., Radulović, M., Milošević, S., Makarov, S.,& Mitić, B.. (2017). Bioaccumulation of metallic trace elements and antioxidant enzyme activities in Apfelbeckia insculpta (L. Koch, 1867) (Diplopoda: Callipodida) from the cave Hadži-Prodanova Pećina (Serbia). in International Journal of Speleology, 46(1), 99-108.
https://doi.org/10.5038/1827-806X.46.1.1981

Vranković J, Borković Mitić S, Ilić B, Radulović M, Milošević S, Makarov S, Mitić B. Bioaccumulation of metallic trace elements and antioxidant enzyme activities in Apfelbeckia insculpta (L. Koch, 1867) (Diplopoda: Callipodida) from the cave Hadži-Prodanova Pećina (Serbia). in International Journal of Speleology. 2017;46(1):99-108.
doi:10.5038/1827-806X.46.1.1981 .

Vranković, Jelena, Borković Mitić, Slavica, Ilić, Bojan, Radulović, Milanka, Milošević, Slaviša, Makarov, Slobodan, Mitić, Bojan, "Bioaccumulation of metallic trace elements and antioxidant enzyme activities in Apfelbeckia insculpta (L. Koch, 1867) (Diplopoda: Callipodida) from the cave Hadži-Prodanova Pećina (Serbia)" in International Journal of Speleology, 46, no. 1 (2017):99-108,
https://doi.org/10.5038/1827-806X.46.1.1981 . .

TY  - JOUR
AU  - Pavlović, Slađan
AU  - Milošević, Slaviša
AU  - Borković Mitić, Slavica
AU  - Simić, Vladica
AU  - Paunović, Momir
AU  - Žikić, Radoslav V.
AU  - Saičić, Zorica
PY  - 2006
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5917
AB  - Spiny cheek crayfish (Orconectes limosus L.) were found in the Serbian part of the River
Danube (August 2004, at 1112 km of the water course). A description of the biology, origin and distribution of this species, as well as the potential impact to the aquatic ecosystems is presented and discussed. This is the first record of this invasive crayfish in Serbian
part of the River Danube. In addition, our results could indicate that there is potential risk
for the expansion of O. limosus in other regional waters in the future.
PB  - Taylor and Francis Group, LLC
T2  - Biotechnology and Biotechnological Equipment
T1  - A Report of Orconectes (Faxonius) Limosus (Rafinesque, 1817) [Crustacea: Decapoda: Astacidea: Cambaridae: Orconectes: Subgenus Faxonius] in the Serbian Part of the River Danube
IS  - 1
VL  - 20
DO  - 10.1080/13102818.2006.10817304
SP  - 53
EP  - 56
ER  - 

@article{
author = "Pavlović, Slađan and Milošević, Slaviša and Borković Mitić, Slavica and Simić, Vladica and Paunović, Momir and Žikić, Radoslav V. and Saičić, Zorica",
year = "2006",
abstract = "Spiny cheek crayfish (Orconectes limosus L.) were found in the Serbian part of the River
Danube (August 2004, at 1112 km of the water course). A description of the biology, origin and distribution of this species, as well as the potential impact to the aquatic ecosystems is presented and discussed. This is the first record of this invasive crayfish in Serbian
part of the River Danube. In addition, our results could indicate that there is potential risk
for the expansion of O. limosus in other regional waters in the future.",
publisher = "Taylor and Francis Group, LLC",
journal = "Biotechnology and Biotechnological Equipment",
title = "A Report of Orconectes (Faxonius) Limosus (Rafinesque, 1817) [Crustacea: Decapoda: Astacidea: Cambaridae: Orconectes: Subgenus Faxonius] in the Serbian Part of the River Danube",
number = "1",
volume = "20",
doi = "10.1080/13102818.2006.10817304",
pages = "53-56"
}

Pavlović, S., Milošević, S., Borković Mitić, S., Simić, V., Paunović, M., Žikić, R. V.,& Saičić, Z.. (2006). A Report of Orconectes (Faxonius) Limosus (Rafinesque, 1817) [Crustacea: Decapoda: Astacidea: Cambaridae: Orconectes: Subgenus Faxonius] in the Serbian Part of the River Danube. in Biotechnology and Biotechnological Equipment
Taylor and Francis Group, LLC., 20(1), 53-56.
https://doi.org/10.1080/13102818.2006.10817304

Pavlović S, Milošević S, Borković Mitić S, Simić V, Paunović M, Žikić RV, Saičić Z. A Report of Orconectes (Faxonius) Limosus (Rafinesque, 1817) [Crustacea: Decapoda: Astacidea: Cambaridae: Orconectes: Subgenus Faxonius] in the Serbian Part of the River Danube. in Biotechnology and Biotechnological Equipment. 2006;20(1):53-56.
doi:10.1080/13102818.2006.10817304 .

Pavlović, Slađan, Milošević, Slaviša, Borković Mitić, Slavica, Simić, Vladica, Paunović, Momir, Žikić, Radoslav V., Saičić, Zorica, "A Report of Orconectes (Faxonius) Limosus (Rafinesque, 1817) [Crustacea: Decapoda: Astacidea: Cambaridae: Orconectes: Subgenus Faxonius] in the Serbian Part of the River Danube" in Biotechnology and Biotechnological Equipment, 20, no. 1 (2006):53-56,
https://doi.org/10.1080/13102818.2006.10817304 . .

TY  - CONF
AU  - Borković-Mitić, Slavica
AU  - Milošević, Slaviša
AU  - Radovanović, Tijana
AU  - Pavlović, Slađan
AU  - Štajn, Andraš Š.
AU  - Žikić, Radoslav V.
AU  - Maksimović-Kosanović, Katica
AU  - Saičić, Zorica
PY  - 2005
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5951
AB  - In our experiment we studied the antioxidant enzyme activities (superoxide dismutase-SOD, catalase-CAT, glutathione peroxidase-GSH-Px, glutathione reductase-GR, and glutathione-S-transferase-GST} in hepatopancreas, gills and abdominal muscle of spinycheek crayfish (Orconectes limosus) from the Serbian part of Danube River. The speciments were collected with deep nets and tissue samples were prepared for analisys by standard methods. All enzyme activities were  measured spectrophotometrically and expressed as specific (U/mg proteins) and total (U/g net mass). The results show strong tissue specifity of investigated enzymes. Specific and total SOD activity in hepatopancreas was lower than in gills  and muscle. At the same time, total SOD activity in gills was significantly lower in respect to muscle. The specific and total activity of CAT was significantly higher in hepatopancreas than in gills and muscle, as well as in gills in respect to the muscle. Same trend was obtained for specific and total activity of GSH-Px. In gills of spinycheek crayfish·, specific activity of GR was significantly higher than in hepatopancreas and muscle, as well as in gills in respect to the muscle. Total GR activity was also significantly higher in gills comparing to hepatopancreas and muscle. The specific and total activity of GST was significantly increased in hepatopancreas than in gills and muscle. At the same time, the activity of GST was higher in gills than in muscle. The obtained results show a strong tissue specifity of antioxidant defense enzymes in investigated tissues of spinycheek crayfish which is accompanied with its different metabolic activity.
PB  - Društvo fiziologa Republike Srbije
PB  - Belgrade: Serbian Physiology Society
C3  - Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia
T1  - Activities of antioxidant defense enzyme in some tissues of spinycheek crayfish (Orconectes limosus Rafinesque) from Serbian part of Danube river
SP  - 219
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5951
ER  - 

@conference{
author = "Borković-Mitić, Slavica and Milošević, Slaviša and Radovanović, Tijana and Pavlović, Slađan and Štajn, Andraš Š. and Žikić, Radoslav V. and Maksimović-Kosanović, Katica and Saičić, Zorica",
year = "2005",
abstract = "In our experiment we studied the antioxidant enzyme activities (superoxide dismutase-SOD, catalase-CAT, glutathione peroxidase-GSH-Px, glutathione reductase-GR, and glutathione-S-transferase-GST} in hepatopancreas, gills and abdominal muscle of spinycheek crayfish (Orconectes limosus) from the Serbian part of Danube River. The speciments were collected with deep nets and tissue samples were prepared for analisys by standard methods. All enzyme activities were  measured spectrophotometrically and expressed as specific (U/mg proteins) and total (U/g net mass). The results show strong tissue specifity of investigated enzymes. Specific and total SOD activity in hepatopancreas was lower than in gills  and muscle. At the same time, total SOD activity in gills was significantly lower in respect to muscle. The specific and total activity of CAT was significantly higher in hepatopancreas than in gills and muscle, as well as in gills in respect to the muscle. Same trend was obtained for specific and total activity of GSH-Px. In gills of spinycheek crayfish·, specific activity of GR was significantly higher than in hepatopancreas and muscle, as well as in gills in respect to the muscle. Total GR activity was also significantly higher in gills comparing to hepatopancreas and muscle. The specific and total activity of GST was significantly increased in hepatopancreas than in gills and muscle. At the same time, the activity of GST was higher in gills than in muscle. The obtained results show a strong tissue specifity of antioxidant defense enzymes in investigated tissues of spinycheek crayfish which is accompanied with its different metabolic activity.",
publisher = "Društvo fiziologa Republike Srbije, Belgrade: Serbian Physiology Society",
journal = "Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia",
title = "Activities of antioxidant defense enzyme in some tissues of spinycheek crayfish (Orconectes limosus Rafinesque) from Serbian part of Danube river",
pages = "219",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5951"
}

Borković-Mitić, S., Milošević, S., Radovanović, T., Pavlović, S., Štajn, A. Š., Žikić, R. V., Maksimović-Kosanović, K.,& Saičić, Z.. (2005). Activities of antioxidant defense enzyme in some tissues of spinycheek crayfish (Orconectes limosus Rafinesque) from Serbian part of Danube river. in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia
Društvo fiziologa Republike Srbije., 219.
https://hdl.handle.net/21.15107/rcub_ibiss_5951

Borković-Mitić S, Milošević S, Radovanović T, Pavlović S, Štajn AŠ, Žikić RV, Maksimović-Kosanović K, Saičić Z. Activities of antioxidant defense enzyme in some tissues of spinycheek crayfish (Orconectes limosus Rafinesque) from Serbian part of Danube river. in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia. 2005;:219.
https://hdl.handle.net/21.15107/rcub_ibiss_5951 .

Borković-Mitić, Slavica, Milošević, Slaviša, Radovanović, Tijana, Pavlović, Slađan, Štajn, Andraš Š., Žikić, Radoslav V., Maksimović-Kosanović, Katica, Saičić, Zorica, "Activities of antioxidant defense enzyme in some tissues of spinycheek crayfish (Orconectes limosus Rafinesque) from Serbian part of Danube river" in Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia (2005):219,
https://hdl.handle.net/21.15107/rcub_ibiss_5951 .