TY  - JOUR
AU  - Petković, Filip
AU  - Campbell, Iain L.
AU  - Gonzalez, Berta
AU  - Castellano, Bernardo
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0165572817301637
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2802
AB  - Cerebellar pathology is a frequent feature of multiple sclerosis (MS), a demyelinating and neuroinflammatory disease of the central nervous system (CNS). Interleukin (IL)-6 is a multifunctional cytokine with a potential role in MS. Here we studied cuprizone-induced cerebellar pathology in transgenic mice with astrocyte-targeted production of IL-6 (GFAP-IL6), specifically focusing on demyelination, oligodendrocyte depletion and microglial cell response. Results Over the course of cuprizone treatment, when compared with WT mice, GFAP-IL6Tg showed a reduced demyelination in the deep lateral cerebellar nuclei (LCN). The oligodendrocyte numbers in the LCN were comparable between WT and GFAP-IL6Tg mice after 4–6 weeks of cuprizone treatment, however after the chronic cuprizone treatment (12 weeks) we detected higher numbers of oligodendrocytes in GFAP-IL6Tg mice. Contrary to strong cuprizone-induced microglial activation in the LCN of WT mice, GFAP-IL6Tg mice had minimal cuprizone-induced microglial changes, despite an already existing reactive microgliosis in control GFAP-IL6Tg not present in control WT mice. Conclusions Our results show that chronic transgenic production of IL-6 reduced cuprizone-induced cerebellar demyelination and induced a specific activation state of the resident microglia population (Iba1 + , CD11b + , MHCII + , CD68 − ), likely rendering them less responsive to subsequent injury signals.
T2  - Journal of Neuroimmunology
T1  - Reduced cuprizone-induced cerebellar demyelination in mice with astrocyte-targeted production of IL-6 is associated with chronically activated, but less responsive microglia
VL  - 310
DO  - 10.1016/j.jneuroim.2017.07.003
SP  - 97
EP  - 102
ER  - 

@article{
author = "Petković, Filip and Campbell, Iain L. and Gonzalez, Berta and Castellano, Bernardo",
year = "2017",
abstract = "Cerebellar pathology is a frequent feature of multiple sclerosis (MS), a demyelinating and neuroinflammatory disease of the central nervous system (CNS). Interleukin (IL)-6 is a multifunctional cytokine with a potential role in MS. Here we studied cuprizone-induced cerebellar pathology in transgenic mice with astrocyte-targeted production of IL-6 (GFAP-IL6), specifically focusing on demyelination, oligodendrocyte depletion and microglial cell response. Results Over the course of cuprizone treatment, when compared with WT mice, GFAP-IL6Tg showed a reduced demyelination in the deep lateral cerebellar nuclei (LCN). The oligodendrocyte numbers in the LCN were comparable between WT and GFAP-IL6Tg mice after 4–6 weeks of cuprizone treatment, however after the chronic cuprizone treatment (12 weeks) we detected higher numbers of oligodendrocytes in GFAP-IL6Tg mice. Contrary to strong cuprizone-induced microglial activation in the LCN of WT mice, GFAP-IL6Tg mice had minimal cuprizone-induced microglial changes, despite an already existing reactive microgliosis in control GFAP-IL6Tg not present in control WT mice. Conclusions Our results show that chronic transgenic production of IL-6 reduced cuprizone-induced cerebellar demyelination and induced a specific activation state of the resident microglia population (Iba1 + , CD11b + , MHCII + , CD68 − ), likely rendering them less responsive to subsequent injury signals.",
journal = "Journal of Neuroimmunology",
title = "Reduced cuprizone-induced cerebellar demyelination in mice with astrocyte-targeted production of IL-6 is associated with chronically activated, but less responsive microglia",
volume = "310",
doi = "10.1016/j.jneuroim.2017.07.003",
pages = "97-102"
}

Petković, F., Campbell, I. L., Gonzalez, B.,& Castellano, B.. (2017). Reduced cuprizone-induced cerebellar demyelination in mice with astrocyte-targeted production of IL-6 is associated with chronically activated, but less responsive microglia. in Journal of Neuroimmunology, 310, 97-102.
https://doi.org/10.1016/j.jneuroim.2017.07.003

Petković F, Campbell IL, Gonzalez B, Castellano B. Reduced cuprizone-induced cerebellar demyelination in mice with astrocyte-targeted production of IL-6 is associated with chronically activated, but less responsive microglia. in Journal of Neuroimmunology. 2017;310:97-102.
doi:10.1016/j.jneuroim.2017.07.003 .

Petković, Filip, Campbell, Iain L., Gonzalez, Berta, Castellano, Bernardo, "Reduced cuprizone-induced cerebellar demyelination in mice with astrocyte-targeted production of IL-6 is associated with chronically activated, but less responsive microglia" in Journal of Neuroimmunology, 310 (2017):97-102,
https://doi.org/10.1016/j.jneuroim.2017.07.003 . .

TY  - JOUR
AU  - Petković, Filip
AU  - Castellano, Bernardo
PY  - 2016
UR  - http://www.nrronline.org/text.asp?2016/11/12/1922/195273
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2546
T2  - Neural Regeneration Research
T1  - The role of interleukin-6 in central nervous system demyelination
IS  - 12
VL  - 11
DO  - 10.4103/1673-5374.195273
SP  - 1922
EP  - 1923
ER  - 

@article{
author = "Petković, Filip and Castellano, Bernardo",
year = "2016",
journal = "Neural Regeneration Research",
title = "The role of interleukin-6 in central nervous system demyelination",
number = "12",
volume = "11",
doi = "10.4103/1673-5374.195273",
pages = "1922-1923"
}

Petković, F.,& Castellano, B.. (2016). The role of interleukin-6 in central nervous system demyelination. in Neural Regeneration Research, 11(12), 1922-1923.
https://doi.org/10.4103/1673-5374.195273

Petković F, Castellano B. The role of interleukin-6 in central nervous system demyelination. in Neural Regeneration Research. 2016;11(12):1922-1923.
doi:10.4103/1673-5374.195273 .

Petković, Filip, Castellano, Bernardo, "The role of interleukin-6 in central nervous system demyelination" in Neural Regeneration Research, 11, no. 12 (2016):1922-1923,
https://doi.org/10.4103/1673-5374.195273 . .