TY  - JOUR
AU  - Jurič, Andreja
AU  - Brčić Karačonji, Irena
AU  - Gašić, Uroš
AU  - Milojković Opsenica, Dušanka
AU  - Prđun, Saša
AU  - Bubalo, Dragan
AU  - Lušić, Dražen
AU  - Vahčić, Nada
AU  - Kopjar, Nevenka
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/3/1903
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9916164
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5483
AB  - Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap-Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes-An In Vitro Study
IS  - 3
VL  - 24
DO  - 10.3390/ijms24031903
SP  - 1903
ER  - 

@article{
author = "Jurič, Andreja and Brčić Karačonji, Irena and Gašić, Uroš and Milojković Opsenica, Dušanka and Prđun, Saša and Bubalo, Dragan and Lušić, Dražen and Vahčić, Nada and Kopjar, Nevenka",
year = "2023",
abstract = "Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap-Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes-An In Vitro Study",
number = "3",
volume = "24",
doi = "10.3390/ijms24031903",
pages = "1903"
}

Jurič, A., Brčić Karačonji, I., Gašić, U., Milojković Opsenica, D., Prđun, S., Bubalo, D., Lušić, D., Vahčić, N.,& Kopjar, N.. (2023). Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes-An In Vitro Study. in International Journal of Molecular Sciences
Basel: MDPI., 24(3), 1903.
https://doi.org/10.3390/ijms24031903

Jurič A, Brčić Karačonji I, Gašić U, Milojković Opsenica D, Prđun S, Bubalo D, Lušić D, Vahčić N, Kopjar N. Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes-An In Vitro Study. in International Journal of Molecular Sciences. 2023;24(3):1903.
doi:10.3390/ijms24031903 .

Jurič, Andreja, Brčić Karačonji, Irena, Gašić, Uroš, Milojković Opsenica, Dušanka, Prđun, Saša, Bubalo, Dragan, Lušić, Dražen, Vahčić, Nada, Kopjar, Nevenka, "Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes-An In Vitro Study" in International Journal of Molecular Sciences, 24, no. 3 (2023):1903,
https://doi.org/10.3390/ijms24031903 . .

TY  - JOUR
AU  - Krstić, Đurđa D.
AU  - Ristivojević, Petar M.
AU  - Gašić, Uroš
AU  - Lazović, Mila
AU  - Fotirić Akšić, Milica M.
AU  - Milivojević, Jasminka
AU  - Morlock, Gertrud E.
AU  - Milojković-Opsenica, Dušanka M.
AU  - Trifković, Jelena Đ.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5162
AB  - Considering the health-benefits of berry fruits consumption and increased market demands for food authenticity as one of the most important quality assurances, phenolic profiling by high-performance thin layer chromatography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combined with multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivated berry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry) revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible for the classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and their derivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds proved to be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-based seed-containing product.
PB  - Oxford: Elsevier Sci Ltd.
T2  - Food Chemistry
T1  - Authenticity assessment of cultivated berries via phenolic profiles of seeds
VL  - 402
DO  - 10.1016/j.foodchem.2022.134184
SP  - 134184
ER  - 

@article{
author = "Krstić, Đurđa D. and Ristivojević, Petar M. and Gašić, Uroš and Lazović, Mila and Fotirić Akšić, Milica M. and Milivojević, Jasminka and Morlock, Gertrud E. and Milojković-Opsenica, Dušanka M. and Trifković, Jelena Đ.",
year = "2023",
abstract = "Considering the health-benefits of berry fruits consumption and increased market demands for food authenticity as one of the most important quality assurances, phenolic profiling by high-performance thin layer chromatography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combined with multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivated berry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry) revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible for the classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and their derivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds proved to be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-based seed-containing product.",
publisher = "Oxford: Elsevier Sci Ltd.",
journal = "Food Chemistry",
title = "Authenticity assessment of cultivated berries via phenolic profiles of seeds",
volume = "402",
doi = "10.1016/j.foodchem.2022.134184",
pages = "134184"
}

Krstić, Đ. D., Ristivojević, P. M., Gašić, U., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry
Oxford: Elsevier Sci Ltd.., 402, 134184.
https://doi.org/10.1016/j.foodchem.2022.134184

Krstić ĐD, Ristivojević PM, Gašić U, Lazović M, Fotirić Akšić MM, Milivojević J, Morlock GE, Milojković-Opsenica DM, Trifković JĐ. Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry. 2023;402:134184.
doi:10.1016/j.foodchem.2022.134184 .

Krstić, Đurđa D., Ristivojević, Petar M., Gašić, Uroš, Lazović, Mila, Fotirić Akšić, Milica M., Milivojević, Jasminka, Morlock, Gertrud E., Milojković-Opsenica, Dušanka M., Trifković, Jelena Đ., "Authenticity assessment of cultivated berries via phenolic profiles of seeds" in Food Chemistry, 402 (2023):134184,
https://doi.org/10.1016/j.foodchem.2022.134184 . .

TY  - JOUR
AU  - Horvacki, Nikola
AU  - Andrić, Filip
AU  - Gašić, Uroš
AU  - Đurović, Dejan
AU  - Tešić, Živoslav
AU  - Fotirić Akšić, Milica
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9653822
UR  - https://www.mdpi.com/1420-3049/27/21/7651
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5239
AB  - Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.
PB  - Basel: MDPI
T2  - Molecules
T1  - Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia
IS  - 21
VL  - 27
DO  - 10.3390/molecules27217651
SP  - 7651
ER  - 

@article{
author = "Horvacki, Nikola and Andrić, Filip and Gašić, Uroš and Đurović, Dejan and Tešić, Živoslav and Fotirić Akšić, Milica and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia",
number = "21",
volume = "27",
doi = "10.3390/molecules27217651",
pages = "7651"
}

Horvacki, N., Andrić, F., Gašić, U., Đurović, D., Tešić, Ž., Fotirić Akšić, M.,& Milojković-Opsenica, D.. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. in Molecules
Basel: MDPI., 27(21), 7651.
https://doi.org/10.3390/molecules27217651

Horvacki N, Andrić F, Gašić U, Đurović D, Tešić Ž, Fotirić Akšić M, Milojković-Opsenica D. Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. in Molecules. 2022;27(21):7651.
doi:10.3390/molecules27217651 .

Horvacki, Nikola, Andrić, Filip, Gašić, Uroš, Đurović, Dejan, Tešić, Živoslav, Fotirić Akšić, Milica, Milojković-Opsenica, Dušanka, "Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia" in Molecules, 27, no. 21 (2022):7651,
https://doi.org/10.3390/molecules27217651 . .