TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Simonović, Ana
AU  - Jevremović, Slađana
AU  - Petrić, Marija P
AU  - Dragicević, Ivana C
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I
AU  - Zahajska, Lenka
AU  - Subotić, Angelina
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/954
AB  - Cytokinin oxidase/dehydrogenase (CKX) is the only known enzyme involved in cytokinin catabolism. Genes coding for two Arabidopsis CKX isoforms, AtCKX1 and AtCKX2, were introduced separately into a binary cloning vector, immobilized into Agrobacterium tumefaciens strain GV3101, and introduced into root explants of centaury (Centaurium erythraea Rafn.). The integration of each transgene was confirmed by genomic PCR. Of the total transformed explants, 30 and 28.2 % of the transformants carried AtCKX1 and AtCKX2 transgenes, respectively. Of these transformants, 50 % exhibited expression of the AtCKX1 transgene, while 64 % of transformants exhibited expression of the AtCKX2 transgene. For all analysed AtCKX transgenic centaury lines, as well as for untransformed control plants, CKX activity was higher in roots than in shoots. Expression of AtCKX in most transgenic lines contributed to enhanced levels of CKX activity in root tissues; whereas, only a few lines demonstrated increased CKX activity in shoot tissues compared to those of control plants. Moreover, overexpression of AtCKX resulted in reduced morphogenetic potential in transgenic plants, but did not significantly affect biomass production in comparison to untransformed control plants.
T2  - Plant Cell Tissue and Organ Culture
T1  - Overexpression of Arabidopsis cytokinin oxidase/dehydrogenase genes AtCKX1 and AtCKX2 in transgenic Centaurium erythraea Rafn.
IS  - 2
VL  - 115
SP  - 27
EP  - 150
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_954
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Cingel, Aleksandar and Simonović, Ana and Jevremović, Slađana and Petrić, Marija P and Dragicević, Ivana C and Motyka, Vaclav and Dobrev, Petre I and Zahajska, Lenka and Subotić, Angelina",
year = "2013",
abstract = "Cytokinin oxidase/dehydrogenase (CKX) is the only known enzyme involved in cytokinin catabolism. Genes coding for two Arabidopsis CKX isoforms, AtCKX1 and AtCKX2, were introduced separately into a binary cloning vector, immobilized into Agrobacterium tumefaciens strain GV3101, and introduced into root explants of centaury (Centaurium erythraea Rafn.). The integration of each transgene was confirmed by genomic PCR. Of the total transformed explants, 30 and 28.2 % of the transformants carried AtCKX1 and AtCKX2 transgenes, respectively. Of these transformants, 50 % exhibited expression of the AtCKX1 transgene, while 64 % of transformants exhibited expression of the AtCKX2 transgene. For all analysed AtCKX transgenic centaury lines, as well as for untransformed control plants, CKX activity was higher in roots than in shoots. Expression of AtCKX in most transgenic lines contributed to enhanced levels of CKX activity in root tissues; whereas, only a few lines demonstrated increased CKX activity in shoot tissues compared to those of control plants. Moreover, overexpression of AtCKX resulted in reduced morphogenetic potential in transgenic plants, but did not significantly affect biomass production in comparison to untransformed control plants.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Overexpression of Arabidopsis cytokinin oxidase/dehydrogenase genes AtCKX1 and AtCKX2 in transgenic Centaurium erythraea Rafn.",
number = "2",
volume = "115",
pages = "27-150",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_954"
}

Trifunović-Momčilov, M., Cingel, A., Simonović, A., Jevremović, S., Petrić, M. P., Dragicević, I. C., Motyka, V., Dobrev, P. I., Zahajska, L.,& Subotić, A.. (2013). Overexpression of Arabidopsis cytokinin oxidase/dehydrogenase genes AtCKX1 and AtCKX2 in transgenic Centaurium erythraea Rafn.. in Plant Cell Tissue and Organ Culture, 115(2), 27-150.
https://hdl.handle.net/21.15107/rcub_ibiss_954

Trifunović-Momčilov M, Cingel A, Simonović A, Jevremović S, Petrić MP, Dragicević IC, Motyka V, Dobrev PI, Zahajska L, Subotić A. Overexpression of Arabidopsis cytokinin oxidase/dehydrogenase genes AtCKX1 and AtCKX2 in transgenic Centaurium erythraea Rafn.. in Plant Cell Tissue and Organ Culture. 2013;115(2):27-150.
https://hdl.handle.net/21.15107/rcub_ibiss_954 .

Trifunović-Momčilov, Milana, Cingel, Aleksandar, Simonović, Ana, Jevremović, Slađana, Petrić, Marija P, Dragicević, Ivana C, Motyka, Vaclav, Dobrev, Petre I, Zahajska, Lenka, Subotić, Angelina, "Overexpression of Arabidopsis cytokinin oxidase/dehydrogenase genes AtCKX1 and AtCKX2 in transgenic Centaurium erythraea Rafn." in Plant Cell Tissue and Organ Culture, 115, no. 2 (2013):27-150,
https://hdl.handle.net/21.15107/rcub_ibiss_954 .

TY  - JOUR
AU  - Petrić, Marija P
AU  - Jevremović, Slađana
AU  - Trifunović-Momčilov, Milana
AU  - Tadić, Vojin
AU  - Milošević, Snežana
AU  - Dragičević, Milan B.
AU  - Subotić, Angelina
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/956
AB  - We investigated the effect of low temperature and gibberellic acid (GA(3)) treatment on dormancy in Fritillaria meleagris L. bulbs. Also, we studied the effect of dormancy breaking on the antioxidant enzymes activity. To overcome dormancy, bulbs require a period (4-8 weeks) of exposure to low temperature. Bulbs regenerated in vitro were grown in the dark on medium without growth regulators at the standard (24 A degrees C) or at low temperatures (4 and 15 A degrees C) for 4, 6, 8 and 10 weeks. Bulbs were collected after 3, 4 and 5 weeks of cooling at 4 A degrees C. To investigate the influence of GA(3) on dormancy, bulbs were treated for 24 h with GA(3) solutions with 1, 2 and 3 mg l(-1) concentrations. During the period of growth of bulbs at 4 A degrees C, regeneration of bulbs was very weak, while at 15 A degrees C the number of regenerated bulbs increased significantly. Improved bulb sprouting was achieved by a short treatment with gibberellin. Low temperature also represents a kind of oxidative stress for the plant. The activity of superoxide dismutase, catalase (CAT) and peroxidase (POX) in bulbs of F. meleagris L. grown in vitro and ex vitro increased with decreasing temperature in contrast to glutathione reductase. POX showed generally lower activity than CAT which indicates that major role in the breaking dormancy and preparing bulbs for sprouting have catalases.
T2  - Acta Physiologiae Plantarum
T1  - The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro
IS  - 11
VL  - 35
SP  - 23
EP  - 3236
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_956
ER  - 

@article{
author = "Petrić, Marija P and Jevremović, Slađana and Trifunović-Momčilov, Milana and Tadić, Vojin and Milošević, Snežana and Dragičević, Milan B. and Subotić, Angelina",
year = "2013",
abstract = "We investigated the effect of low temperature and gibberellic acid (GA(3)) treatment on dormancy in Fritillaria meleagris L. bulbs. Also, we studied the effect of dormancy breaking on the antioxidant enzymes activity. To overcome dormancy, bulbs require a period (4-8 weeks) of exposure to low temperature. Bulbs regenerated in vitro were grown in the dark on medium without growth regulators at the standard (24 A degrees C) or at low temperatures (4 and 15 A degrees C) for 4, 6, 8 and 10 weeks. Bulbs were collected after 3, 4 and 5 weeks of cooling at 4 A degrees C. To investigate the influence of GA(3) on dormancy, bulbs were treated for 24 h with GA(3) solutions with 1, 2 and 3 mg l(-1) concentrations. During the period of growth of bulbs at 4 A degrees C, regeneration of bulbs was very weak, while at 15 A degrees C the number of regenerated bulbs increased significantly. Improved bulb sprouting was achieved by a short treatment with gibberellin. Low temperature also represents a kind of oxidative stress for the plant. The activity of superoxide dismutase, catalase (CAT) and peroxidase (POX) in bulbs of F. meleagris L. grown in vitro and ex vitro increased with decreasing temperature in contrast to glutathione reductase. POX showed generally lower activity than CAT which indicates that major role in the breaking dormancy and preparing bulbs for sprouting have catalases.",
journal = "Acta Physiologiae Plantarum",
title = "The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro",
number = "11",
volume = "35",
pages = "23-3236",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_956"
}

Petrić, M. P., Jevremović, S., Trifunović-Momčilov, M., Tadić, V., Milošević, S., Dragičević, M. B.,& Subotić, A.. (2013). The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro. in Acta Physiologiae Plantarum, 35(11), 23-3236.
https://hdl.handle.net/21.15107/rcub_ibiss_956

Petrić MP, Jevremović S, Trifunović-Momčilov M, Tadić V, Milošević S, Dragičević MB, Subotić A. The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro. in Acta Physiologiae Plantarum. 2013;35(11):23-3236.
https://hdl.handle.net/21.15107/rcub_ibiss_956 .

Petrić, Marija P, Jevremović, Slađana, Trifunović-Momčilov, Milana, Tadić, Vojin, Milošević, Snežana, Dragičević, Milan B., Subotić, Angelina, "The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro" in Acta Physiologiae Plantarum, 35, no. 11 (2013):23-3236,
https://hdl.handle.net/21.15107/rcub_ibiss_956 .

TY  - CONF
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Milošević, Snežana
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Petrić, Marija P
AU  - Dragicević, Ivana C
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1397
C3  - In Vitro Cellular & Developmental Biology-Animal
T1  - Genetic Transformation of Centaurium erythraea with AtCKX1 and AtCKX2 Genes Using Agrobacterium tumefaciens
IS  - null
VL  - 46
EP  - S171
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1397
ER  - 

@conference{
author = "Trifunović-Momčilov, Milana and Cingel, Aleksandar and Milošević, Snežana and Subotić, Angelina and Jevremović, Slađana and Petrić, Marija P and Dragicević, Ivana C",
year = "2010",
journal = "In Vitro Cellular & Developmental Biology-Animal",
title = "Genetic Transformation of Centaurium erythraea with AtCKX1 and AtCKX2 Genes Using Agrobacterium tumefaciens",
number = "null",
volume = "46",
pages = "S171",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1397"
}

Trifunović-Momčilov, M., Cingel, A., Milošević, S., Subotić, A., Jevremović, S., Petrić, M. P.,& Dragicević, I. C.. (2010). Genetic Transformation of Centaurium erythraea with AtCKX1 and AtCKX2 Genes Using Agrobacterium tumefaciens. in In Vitro Cellular & Developmental Biology-Animal, 46(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1397

Trifunović-Momčilov M, Cingel A, Milošević S, Subotić A, Jevremović S, Petrić MP, Dragicević IC. Genetic Transformation of Centaurium erythraea with AtCKX1 and AtCKX2 Genes Using Agrobacterium tumefaciens. in In Vitro Cellular & Developmental Biology-Animal. 2010;46(null):null-S171.
https://hdl.handle.net/21.15107/rcub_ibiss_1397 .

Trifunović-Momčilov, Milana, Cingel, Aleksandar, Milošević, Snežana, Subotić, Angelina, Jevremović, Slađana, Petrić, Marija P, Dragicević, Ivana C, "Genetic Transformation of Centaurium erythraea with AtCKX1 and AtCKX2 Genes Using Agrobacterium tumefaciens" in In Vitro Cellular & Developmental Biology-Animal, 46, no. null (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1397 .

TY  - JOUR
AU  - Subotić, Angelina
AU  - Trifunović-Momčilov, Milana
AU  - Jevremović, Slađana
AU  - Petrić, Marija P
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1346
AB  - Direct somatic embryogenesis of Frittilaria meleagris L. was induced using leaf base explants excised from in vitro grown shoots. Somatic embryos occurred at the basal part of leaf explants 4 weeks after culture on a Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) or kinetin (KIN). The highest number of somatic embryos (SEs) were formed (9.74) from leaf explant on MS medium supplemented with 0.1 mg dm(-3) 2,4-D after 4 weeks of culture initiation. An initial exposure to a low concentration of KIN in the medium also enhanced SEs induction. Our observations by light and scanning electron microscopy revealed that SEs originate directly from the epidermal and subepidermal layers of leaf explant. The developmental stages of somatic embryogenesis from the first unequal cell division through the meristematic clusters, multi-cellular globular somatic embryos to the fully formed cotyledonary embryos were determined. After 4 weeks on MS medium without plant growth regulators, SEs developed into bulblets.
T2  - Biologia Plantarum
T1  - Morpho-histological study of direct somatic embryogenesis in endangered species Frittilaria meleagris
IS  - 3
VL  - 54
EP  - 596
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1346
ER  - 

@article{
author = "Subotić, Angelina and Trifunović-Momčilov, Milana and Jevremović, Slađana and Petrić, Marija P",
year = "2010",
abstract = "Direct somatic embryogenesis of Frittilaria meleagris L. was induced using leaf base explants excised from in vitro grown shoots. Somatic embryos occurred at the basal part of leaf explants 4 weeks after culture on a Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) or kinetin (KIN). The highest number of somatic embryos (SEs) were formed (9.74) from leaf explant on MS medium supplemented with 0.1 mg dm(-3) 2,4-D after 4 weeks of culture initiation. An initial exposure to a low concentration of KIN in the medium also enhanced SEs induction. Our observations by light and scanning electron microscopy revealed that SEs originate directly from the epidermal and subepidermal layers of leaf explant. The developmental stages of somatic embryogenesis from the first unequal cell division through the meristematic clusters, multi-cellular globular somatic embryos to the fully formed cotyledonary embryos were determined. After 4 weeks on MS medium without plant growth regulators, SEs developed into bulblets.",
journal = "Biologia Plantarum",
title = "Morpho-histological study of direct somatic embryogenesis in endangered species Frittilaria meleagris",
number = "3",
volume = "54",
pages = "596",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1346"
}

Subotić, A., Trifunović-Momčilov, M., Jevremović, S.,& Petrić, M. P.. (2010). Morpho-histological study of direct somatic embryogenesis in endangered species Frittilaria meleagris. in Biologia Plantarum, 54(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1346

Subotić A, Trifunović-Momčilov M, Jevremović S, Petrić MP. Morpho-histological study of direct somatic embryogenesis in endangered species Frittilaria meleagris. in Biologia Plantarum. 2010;54(3):null-596.
https://hdl.handle.net/21.15107/rcub_ibiss_1346 .

Subotić, Angelina, Trifunović-Momčilov, Milana, Jevremović, Slađana, Petrić, Marija P, "Morpho-histological study of direct somatic embryogenesis in endangered species Frittilaria meleagris" in Biologia Plantarum, 54, no. 3 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1346 .

TY  - JOUR
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Trifunović-Momčilov, Milana
AU  - Petrić, Marija P
AU  - Milosević, Snezana M
AU  - Grubišić, Dragoljub
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1439
AB  - The effects of exogenous gibberellic acid (GA(3)) and paclobutrazol on induction of somatic embryos in wild type and hairy root culture of Centaurium erythraea Gillib. were investigated. Both compounds were incorperated into 1/2 MS medium at 6 concentrations (0.01, 0.03, 0.1, 0.3, 1.0 and 3.0 mu M). Wild type root and hairy root explants cultured in the presence of GA(3) at all tested concentrations under 16-h photoperiod or in the darkness decreased the number of somatic embryos that were produced. Paclobutrazol (0.3 mu M) induced the largest number (19.7, 16.5) of somatic embryos in wild type and hairy root cultures, respectively. Rooting of plants derived from somatic embryos as achieved on 1/2MS medium. These results indicate that paclobutrazol is beneficial for somatic embryo induction and formation in wild type and hairy root culture.
T2  - African Journal of Biotechnology
T1  - The influence of gibberellic acid and paclobutrazol on induction of somatic embryogenesis in wild type and hairy root cultures of Centaurium erythraea Gillib.
IS  - 14
VL  - 8
EP  - 3228
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1439
ER  - 

@article{
author = "Subotić, Angelina and Jevremović, Slađana and Trifunović-Momčilov, Milana and Petrić, Marija P and Milosević, Snezana M and Grubišić, Dragoljub",
year = "2009",
abstract = "The effects of exogenous gibberellic acid (GA(3)) and paclobutrazol on induction of somatic embryos in wild type and hairy root culture of Centaurium erythraea Gillib. were investigated. Both compounds were incorperated into 1/2 MS medium at 6 concentrations (0.01, 0.03, 0.1, 0.3, 1.0 and 3.0 mu M). Wild type root and hairy root explants cultured in the presence of GA(3) at all tested concentrations under 16-h photoperiod or in the darkness decreased the number of somatic embryos that were produced. Paclobutrazol (0.3 mu M) induced the largest number (19.7, 16.5) of somatic embryos in wild type and hairy root cultures, respectively. Rooting of plants derived from somatic embryos as achieved on 1/2MS medium. These results indicate that paclobutrazol is beneficial for somatic embryo induction and formation in wild type and hairy root culture.",
journal = "African Journal of Biotechnology",
title = "The influence of gibberellic acid and paclobutrazol on induction of somatic embryogenesis in wild type and hairy root cultures of Centaurium erythraea Gillib.",
number = "14",
volume = "8",
pages = "3228",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1439"
}

Subotić, A., Jevremović, S., Trifunović-Momčilov, M., Petrić, M. P., Milosević, S. M.,& Grubišić, D.. (2009). The influence of gibberellic acid and paclobutrazol on induction of somatic embryogenesis in wild type and hairy root cultures of Centaurium erythraea Gillib.. in African Journal of Biotechnology, 8(14).
https://hdl.handle.net/21.15107/rcub_ibiss_1439

Subotić A, Jevremović S, Trifunović-Momčilov M, Petrić MP, Milosević SM, Grubišić D. The influence of gibberellic acid and paclobutrazol on induction of somatic embryogenesis in wild type and hairy root cultures of Centaurium erythraea Gillib.. in African Journal of Biotechnology. 2009;8(14):null-3228.
https://hdl.handle.net/21.15107/rcub_ibiss_1439 .

Subotić, Angelina, Jevremović, Slađana, Trifunović-Momčilov, Milana, Petrić, Marija P, Milosević, Snezana M, Grubišić, Dragoljub, "The influence of gibberellic acid and paclobutrazol on induction of somatic embryogenesis in wild type and hairy root cultures of Centaurium erythraea Gillib." in African Journal of Biotechnology, 8, no. 14 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1439 .