TY  - JOUR
AU  - Todorović, Slađana
AU  - Perić, Marija
AU  - Nikolić, Biljana
AU  - Mandić, Boris
AU  - Cvetković, Stefana
AU  - Bogdanović, Milica
AU  - Živković, Suzana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5795
AB  - The aim of this study was to comparatively analyze chemical composition and biological activity of wild- and in vitro grown Rindera umbellata. Explants were cultivated on 0.003–0.3 M sucrose, fructose, or glucose. HPLC-DAD for quantifying rosmarinic (RA) and lithospermic B (LAB) acids and GC-MS/FID for qualitative pyrrolizidine alkaloids (PAs) detection were used. Antioxidant activity (DPPH and ABTS assays) and cytotoxicity (MTT test) were monitored. Identified PAs were 7-angeloyl heliotridane, lindelofine, 7-angeloyl heliotridine, 7-angeloyl-9-(+)-trachelanthylheliotridine, punctanecine, and heliosupine, with higher variability reported in wild-growing samples. Total phenolic contents (TPCs) were comparable in wild-growing and in vitro samples, but total flavonoid (TFC) and RA levels were multifold higher in in vitro samples. Notably, high concentration of LAB was detected in wild-growing roots. Amounts of 0.3 M and 0.1 M of sucrose were optimal for TFC and RA production, while maximal antioxidant activity was monitored in plants grown on 0.3 M sucrose. The MTT test indicated colorectal HT-29 as more sensitive than A549 lung adenocarcinoma and normal MRC-5 cells, showing selective sensitivity to wild-growing and 0.3 M sucrose samples. In conclusion, PAs in vitro, as well as TPC, TFC, RA, and LAB in both growing conditions were detected for the first time in R. umbellata.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge
IS  - 3
VL  - 9
DO  - 10.3390/horticulturae9030381
SP  - 381
ER  - 

@article{
author = "Todorović, Slađana and Perić, Marija and Nikolić, Biljana and Mandić, Boris and Cvetković, Stefana and Bogdanović, Milica and Živković, Suzana",
year = "2023",
abstract = "The aim of this study was to comparatively analyze chemical composition and biological activity of wild- and in vitro grown Rindera umbellata. Explants were cultivated on 0.003–0.3 M sucrose, fructose, or glucose. HPLC-DAD for quantifying rosmarinic (RA) and lithospermic B (LAB) acids and GC-MS/FID for qualitative pyrrolizidine alkaloids (PAs) detection were used. Antioxidant activity (DPPH and ABTS assays) and cytotoxicity (MTT test) were monitored. Identified PAs were 7-angeloyl heliotridane, lindelofine, 7-angeloyl heliotridine, 7-angeloyl-9-(+)-trachelanthylheliotridine, punctanecine, and heliosupine, with higher variability reported in wild-growing samples. Total phenolic contents (TPCs) were comparable in wild-growing and in vitro samples, but total flavonoid (TFC) and RA levels were multifold higher in in vitro samples. Notably, high concentration of LAB was detected in wild-growing roots. Amounts of 0.3 M and 0.1 M of sucrose were optimal for TFC and RA production, while maximal antioxidant activity was monitored in plants grown on 0.3 M sucrose. The MTT test indicated colorectal HT-29 as more sensitive than A549 lung adenocarcinoma and normal MRC-5 cells, showing selective sensitivity to wild-growing and 0.3 M sucrose samples. In conclusion, PAs in vitro, as well as TPC, TFC, RA, and LAB in both growing conditions were detected for the first time in R. umbellata.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge",
number = "3",
volume = "9",
doi = "10.3390/horticulturae9030381",
pages = "381"
}

Todorović, S., Perić, M., Nikolić, B., Mandić, B., Cvetković, S., Bogdanović, M.,& Živković, S.. (2023). Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge. in Horticulturae
Basel: MDPI., 9(3), 381.
https://doi.org/10.3390/horticulturae9030381

Todorović S, Perić M, Nikolić B, Mandić B, Cvetković S, Bogdanović M, Živković S. Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge. in Horticulturae. 2023;9(3):381.
doi:10.3390/horticulturae9030381 .

Todorović, Slađana, Perić, Marija, Nikolić, Biljana, Mandić, Boris, Cvetković, Stefana, Bogdanović, Milica, Živković, Suzana, "Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge" in Horticulturae, 9, no. 3 (2023):381,
https://doi.org/10.3390/horticulturae9030381 . .

TY  - JOUR
AU  - Fischedick, Justin T
AU  - Pešić, Milica
AU  - Podolski-Renić, Ana
AU  - Banković, Jasna Z.
AU  - de Vos, Ric CH
AU  - Perić, Marija
AU  - Todorović, Slađana
AU  - Tanić, Nikola T
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1016
AB  - Five new sesquiterpene lactones (1-5) were isolated from Inula britannica collected in the wild from Serbia along with five known compounds (6-10). Sesquiterpene lactones were isolated using centrifugal partition chromatography followed by combination of flash chromatography and semi-preparative HPLC. Isolated compounds were screened for cytotoxic activity on four different human cancer cell lines and their multi-drug resistant counterparts, as well as on normal human keratinocytes. Sesquiterpene lactones showed similar cytotoxic activity toward drug sensitive and drug resistant cancer cell lines. (C) 2013 Phytochemical Society of Europe. Published by Elsevier B. V. All rights reserved.
T2  - Phytochemistry Letters
T1  - Cytotoxic activity of sesquiterpene lactones from Inula britannica on human cancer cell lines
IS  - 2
VL  - 6
SP  - 1
EP  - 252
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1016
ER  - 

@article{
author = "Fischedick, Justin T and Pešić, Milica and Podolski-Renić, Ana and Banković, Jasna Z. and de Vos, Ric CH and Perić, Marija and Todorović, Slađana and Tanić, Nikola T",
year = "2013",
abstract = "Five new sesquiterpene lactones (1-5) were isolated from Inula britannica collected in the wild from Serbia along with five known compounds (6-10). Sesquiterpene lactones were isolated using centrifugal partition chromatography followed by combination of flash chromatography and semi-preparative HPLC. Isolated compounds were screened for cytotoxic activity on four different human cancer cell lines and their multi-drug resistant counterparts, as well as on normal human keratinocytes. Sesquiterpene lactones showed similar cytotoxic activity toward drug sensitive and drug resistant cancer cell lines. (C) 2013 Phytochemical Society of Europe. Published by Elsevier B. V. All rights reserved.",
journal = "Phytochemistry Letters",
title = "Cytotoxic activity of sesquiterpene lactones from Inula britannica on human cancer cell lines",
number = "2",
volume = "6",
pages = "1-252",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1016"
}

Fischedick, J. T., Pešić, M., Podolski-Renić, A., Banković, J. Z., de Vos, R. C., Perić, M., Todorović, S.,& Tanić, N. T.. (2013). Cytotoxic activity of sesquiterpene lactones from Inula britannica on human cancer cell lines. in Phytochemistry Letters, 6(2), 1-252.
https://hdl.handle.net/21.15107/rcub_ibiss_1016

Fischedick JT, Pešić M, Podolski-Renić A, Banković JZ, de Vos RC, Perić M, Todorović S, Tanić NT. Cytotoxic activity of sesquiterpene lactones from Inula britannica on human cancer cell lines. in Phytochemistry Letters. 2013;6(2):1-252.
https://hdl.handle.net/21.15107/rcub_ibiss_1016 .

Fischedick, Justin T, Pešić, Milica, Podolski-Renić, Ana, Banković, Jasna Z., de Vos, Ric CH, Perić, Marija, Todorović, Slađana, Tanić, Nikola T, "Cytotoxic activity of sesquiterpene lactones from Inula britannica on human cancer cell lines" in Phytochemistry Letters, 6, no. 2 (2013):1-252,
https://hdl.handle.net/21.15107/rcub_ibiss_1016 .

TY  - JOUR
AU  - Perić, Marija
AU  - Dmitrović, Slavica
AU  - Živković, Suzana
AU  - Filipović, Biljana
AU  - Skorić, Marijana
AU  - Simonović, Ana
AU  - Todorović, Slađana
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1153
AB  - Rindera umbellata (Waldst. & Kit.) Bunge is a rare, critically endangered and horticulturally appealing plant with unexplored pharmaceutical potential. Its distribution is restricted to sandy habitats, whereas propagation in nature is limited by fungal infections of the seeds. To initiate its ex situ conservation and provide material for metabolomic studies, we have introduced R. umbellata into in vitro culture using immature embryos as primary explants. A 72% of the embryos germinated on growth regulator-free medium. The optimization of growth conditions was based on varying carbohydrates (sucrose, glucose, and fructose) in the medium. In vitro growth and development of R. umbellata plants were significantly affected by both the type and concentration of the applied sugars. For most recorded parameters, including leaf elongation, biomass production, rooting percentage, and the number and length of roots, 0.1 M sucrose was optimal. The highest percentage of explants with developed buds was achieved on 0.06 M sucrose (38.77%) or 0.3 M glucose (27.43%). The plantlets obtained on 0.1 M sucrose were successfully acclimatized to greenhouse and field conditions with survival rates of 71.43% and 42.86%, respectively. To our best knowledge, this is the first publication dedicated to this species.
T2  - Hortscience
T1  - In Vitro Growth, Morphogenesis, and Acclimatization of Endangered Rindera umbellata (Waldst. & Kit.) Bunge
IS  - 8
VL  - 47
SP  - 27
EP  - 1128
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1153
ER  - 

@article{
author = "Perić, Marija and Dmitrović, Slavica and Živković, Suzana and Filipović, Biljana and Skorić, Marijana and Simonović, Ana and Todorović, Slađana",
year = "2012",
abstract = "Rindera umbellata (Waldst. & Kit.) Bunge is a rare, critically endangered and horticulturally appealing plant with unexplored pharmaceutical potential. Its distribution is restricted to sandy habitats, whereas propagation in nature is limited by fungal infections of the seeds. To initiate its ex situ conservation and provide material for metabolomic studies, we have introduced R. umbellata into in vitro culture using immature embryos as primary explants. A 72% of the embryos germinated on growth regulator-free medium. The optimization of growth conditions was based on varying carbohydrates (sucrose, glucose, and fructose) in the medium. In vitro growth and development of R. umbellata plants were significantly affected by both the type and concentration of the applied sugars. For most recorded parameters, including leaf elongation, biomass production, rooting percentage, and the number and length of roots, 0.1 M sucrose was optimal. The highest percentage of explants with developed buds was achieved on 0.06 M sucrose (38.77%) or 0.3 M glucose (27.43%). The plantlets obtained on 0.1 M sucrose were successfully acclimatized to greenhouse and field conditions with survival rates of 71.43% and 42.86%, respectively. To our best knowledge, this is the first publication dedicated to this species.",
journal = "Hortscience",
title = "In Vitro Growth, Morphogenesis, and Acclimatization of Endangered Rindera umbellata (Waldst. & Kit.) Bunge",
number = "8",
volume = "47",
pages = "27-1128",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1153"
}

Perić, M., Dmitrović, S., Živković, S., Filipović, B., Skorić, M., Simonović, A.,& Todorović, S.. (2012). In Vitro Growth, Morphogenesis, and Acclimatization of Endangered Rindera umbellata (Waldst. & Kit.) Bunge. in Hortscience, 47(8), 27-1128.
https://hdl.handle.net/21.15107/rcub_ibiss_1153

Perić M, Dmitrović S, Živković S, Filipović B, Skorić M, Simonović A, Todorović S. In Vitro Growth, Morphogenesis, and Acclimatization of Endangered Rindera umbellata (Waldst. & Kit.) Bunge. in Hortscience. 2012;47(8):27-1128.
https://hdl.handle.net/21.15107/rcub_ibiss_1153 .

Perić, Marija, Dmitrović, Slavica, Živković, Suzana, Filipović, Biljana, Skorić, Marijana, Simonović, Ana, Todorović, Slađana, "In Vitro Growth, Morphogenesis, and Acclimatization of Endangered Rindera umbellata (Waldst. & Kit.) Bunge" in Hortscience, 47, no. 8 (2012):27-1128,
https://hdl.handle.net/21.15107/rcub_ibiss_1153 .