TY  - JOUR
AU  - Nikodijević, Danijela D.
AU  - Jovankić, Jovana V.
AU  - Cvetković, Danijela M.
AU  - Anđelković, Marko
AU  - Nikezić, Aleksandra G.
AU  - Milutinović, Milena G.
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0014299921006208
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4481
AB  - This study evaluated the potential of antitumor activity of snake venom from Vipera ammodytes and L-amino acid oxidase from Crotalus adamanteus on different colorectal cancer cell lines through determination of cytotoxic activity by MTT assay, pro-apoptotic activity by acridine orange/ethidium bromide staining, and concentrations of redox status parameters (superoxide, reduced glutathione, lipid peroxidation) by colorimetric methods. The expression of genes involved in the biotransformation process and metabolite efflux was determined by qPCR method, while protein expression of glutathione synthetase and P-glycoprotein were analysed by immunocytochemistry. The analysis of cell death shows that snake venom dominantly leads cells to necrosis. Induction of apoptosis by L-amino acid oxidase was in correlation with oxidative disbalance in cancer cells. Gene expression profile of membrane transporters and CYP genes were different in each cell line and in correlation with their sensitivity of treatment. Our results show that L-amino acid oxidase from snake venom is a potent cytotoxic substance with pronounced pro-apoptotic activity. The inhibition of P-glycoprotein suggests that L-amino acid oxidase is a good substance for furter research of antitumor effect, with unexpressed potential for occurrence of drug resistance in vitro.
PB  - Elsevier B.V.
T2  - European Journal of Pharmacology
T1  - L-amino acid oxidase from snake venom: Biotransformation and induction of apoptosis in human colon cancer cells.
VL  - 910
DO  - 10.1016/j.ejphar.2021.174466
SP  - 174466
ER  - 

@article{
author = "Nikodijević, Danijela D. and Jovankić, Jovana V. and Cvetković, Danijela M. and Anđelković, Marko and Nikezić, Aleksandra G. and Milutinović, Milena G.",
year = "2021",
abstract = "This study evaluated the potential of antitumor activity of snake venom from Vipera ammodytes and L-amino acid oxidase from Crotalus adamanteus on different colorectal cancer cell lines through determination of cytotoxic activity by MTT assay, pro-apoptotic activity by acridine orange/ethidium bromide staining, and concentrations of redox status parameters (superoxide, reduced glutathione, lipid peroxidation) by colorimetric methods. The expression of genes involved in the biotransformation process and metabolite efflux was determined by qPCR method, while protein expression of glutathione synthetase and P-glycoprotein were analysed by immunocytochemistry. The analysis of cell death shows that snake venom dominantly leads cells to necrosis. Induction of apoptosis by L-amino acid oxidase was in correlation with oxidative disbalance in cancer cells. Gene expression profile of membrane transporters and CYP genes were different in each cell line and in correlation with their sensitivity of treatment. Our results show that L-amino acid oxidase from snake venom is a potent cytotoxic substance with pronounced pro-apoptotic activity. The inhibition of P-glycoprotein suggests that L-amino acid oxidase is a good substance for furter research of antitumor effect, with unexpressed potential for occurrence of drug resistance in vitro.",
publisher = "Elsevier B.V.",
journal = "European Journal of Pharmacology",
title = "L-amino acid oxidase from snake venom: Biotransformation and induction of apoptosis in human colon cancer cells.",
volume = "910",
doi = "10.1016/j.ejphar.2021.174466",
pages = "174466"
}

Nikodijević, D. D., Jovankić, J. V., Cvetković, D. M., Anđelković, M., Nikezić, A. G.,& Milutinović, M. G.. (2021). L-amino acid oxidase from snake venom: Biotransformation and induction of apoptosis in human colon cancer cells.. in European Journal of Pharmacology
Elsevier B.V.., 910, 174466.
https://doi.org/10.1016/j.ejphar.2021.174466

Nikodijević DD, Jovankić JV, Cvetković DM, Anđelković M, Nikezić AG, Milutinović MG. L-amino acid oxidase from snake venom: Biotransformation and induction of apoptosis in human colon cancer cells.. in European Journal of Pharmacology. 2021;910:174466.
doi:10.1016/j.ejphar.2021.174466 .

Nikodijević, Danijela D., Jovankić, Jovana V., Cvetković, Danijela M., Anđelković, Marko, Nikezić, Aleksandra G., Milutinović, Milena G., "L-amino acid oxidase from snake venom: Biotransformation and induction of apoptosis in human colon cancer cells." in European Journal of Pharmacology, 910 (2021):174466,
https://doi.org/10.1016/j.ejphar.2021.174466 . .