TY  - JOUR
AU  - Mićanović, Dragica
AU  - Stojanović, Ivana D.
AU  - Koprivica, Ivan
AU  - Pejnović, Nada
AU  - Šavikin, Katarina
AU  - Ćujić-Nikolić, Nada
AU  - Saksida, Tamara
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5021
AB  - Chokeberry has exhibited cardioprotective, anti-bacterial, immunomodulating and anti-cancer properties. Chokeberry extract (CE) was tested in the model of melanoma induced by B16 cells inoculation in C57BL/6 mice.

CE treatment that began 7 days before inoculation and continued through the observation period, delayed melanoma appearance and increased infiltration of immune cells in the tumor microenvironment (TME). Levels of TNF, perforin, granzyme B and IL-1β did not differ between the CE-treated and control animals, but the TME of CE-treated mice contained more IFN-γ-producing cells and a lesser frequency of CCR5-expressing MDSC. In vitro, CE displayed no direct cytotoxicity to B16 cells. However, splenocytes isolated from CE-treated animals exerted strong cytotoxic effect on B16 cells in vitro. Neutralization of IFN-γ diminished the observed B16 death, suggesting that this effect was mediated mainly by splenocyte-derived IFN-γ.

In conclusion, pre-treatment with CE stimulated the anti-tumor immune response by enhancing IFN-γ-producing cells to act against melanoma.
PB  - Amsterdam : Elsevier
T2  - Journal of Functional Foods
T1  - Chokeberry (Aronia melanocarpa) fruit extract abrogates melanoma progression through boosting up IFN-γ-producing cells
VL  - 95
DO  - 10.1016/j.jff.2022.105185
SP  - 105185
ER  - 

@article{
author = "Mićanović, Dragica and Stojanović, Ivana D. and Koprivica, Ivan and Pejnović, Nada and Šavikin, Katarina and Ćujić-Nikolić, Nada and Saksida, Tamara",
year = "2022",
abstract = "Chokeberry has exhibited cardioprotective, anti-bacterial, immunomodulating and anti-cancer properties. Chokeberry extract (CE) was tested in the model of melanoma induced by B16 cells inoculation in C57BL/6 mice.

CE treatment that began 7 days before inoculation and continued through the observation period, delayed melanoma appearance and increased infiltration of immune cells in the tumor microenvironment (TME). Levels of TNF, perforin, granzyme B and IL-1β did not differ between the CE-treated and control animals, but the TME of CE-treated mice contained more IFN-γ-producing cells and a lesser frequency of CCR5-expressing MDSC. In vitro, CE displayed no direct cytotoxicity to B16 cells. However, splenocytes isolated from CE-treated animals exerted strong cytotoxic effect on B16 cells in vitro. Neutralization of IFN-γ diminished the observed B16 death, suggesting that this effect was mediated mainly by splenocyte-derived IFN-γ.

In conclusion, pre-treatment with CE stimulated the anti-tumor immune response by enhancing IFN-γ-producing cells to act against melanoma.",
publisher = "Amsterdam : Elsevier",
journal = "Journal of Functional Foods",
title = "Chokeberry (Aronia melanocarpa) fruit extract abrogates melanoma progression through boosting up IFN-γ-producing cells",
volume = "95",
doi = "10.1016/j.jff.2022.105185",
pages = "105185"
}

Mićanović, D., Stojanović, I. D., Koprivica, I., Pejnović, N., Šavikin, K., Ćujić-Nikolić, N.,& Saksida, T.. (2022). Chokeberry (Aronia melanocarpa) fruit extract abrogates melanoma progression through boosting up IFN-γ-producing cells. in Journal of Functional Foods
Amsterdam : Elsevier., 95, 105185.
https://doi.org/10.1016/j.jff.2022.105185

Mićanović D, Stojanović ID, Koprivica I, Pejnović N, Šavikin K, Ćujić-Nikolić N, Saksida T. Chokeberry (Aronia melanocarpa) fruit extract abrogates melanoma progression through boosting up IFN-γ-producing cells. in Journal of Functional Foods. 2022;95:105185.
doi:10.1016/j.jff.2022.105185 .

Mićanović, Dragica, Stojanović, Ivana D., Koprivica, Ivan, Pejnović, Nada, Šavikin, Katarina, Ćujić-Nikolić, Nada, Saksida, Tamara, "Chokeberry (Aronia melanocarpa) fruit extract abrogates melanoma progression through boosting up IFN-γ-producing cells" in Journal of Functional Foods, 95 (2022):105185,
https://doi.org/10.1016/j.jff.2022.105185 . .

TY  - CONF
AU  - Vujičić, Milorad
AU  - Sabovljević, Marko
AU  - Milošević, Snežana
AU  - Nikolić, Nada
AU  - Sabovljević, Aneta
PY  - 2014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6792
AB  - The moss Enthosthodon hungaricus is a rare and threatened species in Europe. It is considered as a facultative moss halophyte that inhabits the salty enviroment in central, easten and southern Europe. Here, we studied axenically farmed, in vitro developed gametophyte tolerance to NaCl stress. The salt stress strongly affects plant growth and development. We compared E. hungaricus with another funeroid annual moss Physcomitrella patens on salt tolerance. To observe the stress on bryophyte development in vitro, basal medium was enriched with NaCl (0, 5, 10, 50, 100, 200, 300, 500 mM NaCl). Proline and free amino acides have a role in protein synthesis and stress response in plants. The endogenous proline accumulation in salt stressed plants has been utilized as effective indicator for salt tolerance. It was recorded that increase of the salt concentration up to 200 mM influence the increase of the concentration of proline. In plants grown at the highest salt concentrations (300 and 500 mM), a significant decrease in proline values was noticed.
PB  - Plovdiv, Bulgaria: Plovdiv University Press "Paisii Hilendarski"
C3  - Programme and abstracts: 3rd Balkan Scientific Conference on Biology; 2014 May 30 - Jun 1; Plovdiv, Bulgraria
T1  - Effects of NaCl on proline and free amino acid concentration in bryophytes Entosthodon humgaricus and Physcomitrela patens in vitro
EP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6792
ER  - 

@conference{
author = "Vujičić, Milorad and Sabovljević, Marko and Milošević, Snežana and Nikolić, Nada and Sabovljević, Aneta",
year = "2014",
abstract = "The moss Enthosthodon hungaricus is a rare and threatened species in Europe. It is considered as a facultative moss halophyte that inhabits the salty enviroment in central, easten and southern Europe. Here, we studied axenically farmed, in vitro developed gametophyte tolerance to NaCl stress. The salt stress strongly affects plant growth and development. We compared E. hungaricus with another funeroid annual moss Physcomitrella patens on salt tolerance. To observe the stress on bryophyte development in vitro, basal medium was enriched with NaCl (0, 5, 10, 50, 100, 200, 300, 500 mM NaCl). Proline and free amino acides have a role in protein synthesis and stress response in plants. The endogenous proline accumulation in salt stressed plants has been utilized as effective indicator for salt tolerance. It was recorded that increase of the salt concentration up to 200 mM influence the increase of the concentration of proline. In plants grown at the highest salt concentrations (300 and 500 mM), a significant decrease in proline values was noticed.",
publisher = "Plovdiv, Bulgaria: Plovdiv University Press "Paisii Hilendarski"",
journal = "Programme and abstracts: 3rd Balkan Scientific Conference on Biology; 2014 May 30 - Jun 1; Plovdiv, Bulgraria",
title = "Effects of NaCl on proline and free amino acid concentration in bryophytes Entosthodon humgaricus and Physcomitrela patens in vitro",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6792"
}

Vujičić, M., Sabovljević, M., Milošević, S., Nikolić, N.,& Sabovljević, A.. (2014). Effects of NaCl on proline and free amino acid concentration in bryophytes Entosthodon humgaricus and Physcomitrela patens in vitro. in Programme and abstracts: 3rd Balkan Scientific Conference on Biology; 2014 May 30 - Jun 1; Plovdiv, Bulgraria
Plovdiv, Bulgaria: Plovdiv University Press "Paisii Hilendarski"..
https://hdl.handle.net/21.15107/rcub_ibiss_6792

Vujičić M, Sabovljević M, Milošević S, Nikolić N, Sabovljević A. Effects of NaCl on proline and free amino acid concentration in bryophytes Entosthodon humgaricus and Physcomitrela patens in vitro. in Programme and abstracts: 3rd Balkan Scientific Conference on Biology; 2014 May 30 - Jun 1; Plovdiv, Bulgraria. 2014;:null-73.
https://hdl.handle.net/21.15107/rcub_ibiss_6792 .

Vujičić, Milorad, Sabovljević, Marko, Milošević, Snežana, Nikolić, Nada, Sabovljević, Aneta, "Effects of NaCl on proline and free amino acid concentration in bryophytes Entosthodon humgaricus and Physcomitrela patens in vitro" in Programme and abstracts: 3rd Balkan Scientific Conference on Biology; 2014 May 30 - Jun 1; Plovdiv, Bulgraria (2014),
https://hdl.handle.net/21.15107/rcub_ibiss_6792 .