TY  - JOUR
AU  - Popržen, Tatjana
AU  - Jevremović, Slađana
AU  - Milošević, Snežana
AU  - Đurić, Marija
AU  - Uzelac, Branka
AU  - Stanković, Slaviša
AU  - Radulović, Olga
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6796
AB  - Aquatic plants, just like terrestrial plants, are exposed to oxidative stress. However,
their responses are still under-researched. In this study, we examined the physiological and antioxidative
responses of an aquatic plant, duckweed (Lemna minor L.), to four indole-3-acetic acid
(IAA)-degrading and -producing Pseudomonas bacteria (Pseudomonas oryzihabitans D1-104/3, P. putida
A3-104/5, P. gessardii C31-106/3 and P. yamanorum C44-104/1) and/or a supraphysiological level
of IAA (10 mg L−1). Growth characteristics, total photosynthetic pigment content, histochemical
localization of reactive oxygen species and antioxidant enzyme activity (SOD, CAT and POX) were
evaluated at two time points, after 3 and 7 days of co-cultivation. Superoxide anion and hydrogen
peroxide were produced and accumulated mainly in the roots, daughter fronds and veins of duckweeds.
Duckweeds’ responses depended on the strain of Pseudomonas, time and exogenous IAA.
Co-cultivation of duckweed with bacteria has positive or neutral effects. Exogenous application
of IAA had a negative or neutral effect on enzyme activity and other parameters. Co-cultivation
with P. gessardii C31-106/3 showed plant-growth-promoting effects on duckweed: increased biomass
production, modulation of duckweeds’ antioxidant enzymatic activity and reduction in hydrogen
peroxide content. This study widens our knowledge of aquatic plants and their response to oxidative
stress, supports the hypothesis that plant growth-promoting bacteria (PGPB) induce tolerable levels
of oxidative stress in plants and introduces a new PGPB strain, P. gessardii C31-106/3.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid
IS  - 6
VL  - 10
DO  - 10.3390/horticulturae10060562
SP  - 562
ER  - 

@article{
author = "Popržen, Tatjana and Jevremović, Slađana and Milošević, Snežana and Đurić, Marija and Uzelac, Branka and Stanković, Slaviša and Radulović, Olga",
year = "2024",
abstract = "Aquatic plants, just like terrestrial plants, are exposed to oxidative stress. However,
their responses are still under-researched. In this study, we examined the physiological and antioxidative
responses of an aquatic plant, duckweed (Lemna minor L.), to four indole-3-acetic acid
(IAA)-degrading and -producing Pseudomonas bacteria (Pseudomonas oryzihabitans D1-104/3, P. putida
A3-104/5, P. gessardii C31-106/3 and P. yamanorum C44-104/1) and/or a supraphysiological level
of IAA (10 mg L−1). Growth characteristics, total photosynthetic pigment content, histochemical
localization of reactive oxygen species and antioxidant enzyme activity (SOD, CAT and POX) were
evaluated at two time points, after 3 and 7 days of co-cultivation. Superoxide anion and hydrogen
peroxide were produced and accumulated mainly in the roots, daughter fronds and veins of duckweeds.
Duckweeds’ responses depended on the strain of Pseudomonas, time and exogenous IAA.
Co-cultivation of duckweed with bacteria has positive or neutral effects. Exogenous application
of IAA had a negative or neutral effect on enzyme activity and other parameters. Co-cultivation
with P. gessardii C31-106/3 showed plant-growth-promoting effects on duckweed: increased biomass
production, modulation of duckweeds’ antioxidant enzymatic activity and reduction in hydrogen
peroxide content. This study widens our knowledge of aquatic plants and their response to oxidative
stress, supports the hypothesis that plant growth-promoting bacteria (PGPB) induce tolerable levels
of oxidative stress in plants and introduces a new PGPB strain, P. gessardii C31-106/3.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid",
number = "6",
volume = "10",
doi = "10.3390/horticulturae10060562",
pages = "562"
}

Popržen, T., Jevremović, S., Milošević, S., Đurić, M., Uzelac, B., Stanković, S.,& Radulović, O.. (2024). Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid. in Horticulturae
Basel: MDPI., 10(6), 562.
https://doi.org/10.3390/horticulturae10060562

Popržen T, Jevremović S, Milošević S, Đurić M, Uzelac B, Stanković S, Radulović O. Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid. in Horticulturae. 2024;10(6):562.
doi:10.3390/horticulturae10060562 .

Popržen, Tatjana, Jevremović, Slađana, Milošević, Snežana, Đurić, Marija, Uzelac, Branka, Stanković, Slaviša, Radulović, Olga, "Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid" in Horticulturae, 10, no. 6 (2024):562,
https://doi.org/10.3390/horticulturae10060562 . .

TY  - JOUR
AU  - Popržen, Tatjana
AU  - Nikolić, Ivan
AU  - Krstić-Milošević, Dijana
AU  - Uzelac, Branka
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
AU  - Radulović, Olga
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6442
AB  - The rhizosphere represents a center of complex and dynamic interactions between plants and microbes, resulting in various positive effects on plant growth and development. However, less is known about the effects of indole-3-acetic acid (IAA) on aquatic plants. In this study, we report the characterization of four Pseudomonas strains isolated from the rhizosphere of the common duckweed (Lemna minor) with IAA-degradation and -utilization ability. Our results confirm previous reports on the negative effect of IAA on aquatic plants, contrary to the effect on terrestrial plants. P. putida A3-104/5 demonstrated particularly beneficial traits, as it exhibited not only IAA-degrading and -producing activity but also a positive effect on the doubling time of duckweeds in the presence of IAA, positive chemotaxis in the presence of IAA, increased tolerance to oxidative stress in the presence of IAA and increased biofilm formation related to IAA. Similarly, P. gessardii C31-106/3 significantly shortened the doubling time of duckweeds in the presence of IAA, while having a neutral effect in the absence of IAA. These traits are important in the context of plant–bacteria interactions and highlight the role of IAA as a common metabolite in these interactions, especially in aquatic environments where plants are facing unique challenges compared to their terrestrial counterparts. We conclude that IAA-degrading and -producing strains presented in this study might regulate IAA effects on aquatic plants and confer evolutionary benefits under adverse conditions (e.g., under oxidative stress, excess of IAA or nutrient scarcity).
PB  - Basel : MDPI
T2  - International Journal of Molecular Sciences
T1  - Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)
IS  - 24
VL  - 24
DO  - 10.3390/ijms242417207
SP  - 17207
ER  - 

@article{
author = "Popržen, Tatjana and Nikolić, Ivan and Krstić-Milošević, Dijana and Uzelac, Branka and Trifunović-Momčilov, Milana and Marković, Marija and Radulović, Olga",
year = "2023",
abstract = "The rhizosphere represents a center of complex and dynamic interactions between plants and microbes, resulting in various positive effects on plant growth and development. However, less is known about the effects of indole-3-acetic acid (IAA) on aquatic plants. In this study, we report the characterization of four Pseudomonas strains isolated from the rhizosphere of the common duckweed (Lemna minor) with IAA-degradation and -utilization ability. Our results confirm previous reports on the negative effect of IAA on aquatic plants, contrary to the effect on terrestrial plants. P. putida A3-104/5 demonstrated particularly beneficial traits, as it exhibited not only IAA-degrading and -producing activity but also a positive effect on the doubling time of duckweeds in the presence of IAA, positive chemotaxis in the presence of IAA, increased tolerance to oxidative stress in the presence of IAA and increased biofilm formation related to IAA. Similarly, P. gessardii C31-106/3 significantly shortened the doubling time of duckweeds in the presence of IAA, while having a neutral effect in the absence of IAA. These traits are important in the context of plant–bacteria interactions and highlight the role of IAA as a common metabolite in these interactions, especially in aquatic environments where plants are facing unique challenges compared to their terrestrial counterparts. We conclude that IAA-degrading and -producing strains presented in this study might regulate IAA effects on aquatic plants and confer evolutionary benefits under adverse conditions (e.g., under oxidative stress, excess of IAA or nutrient scarcity).",
publisher = "Basel : MDPI",
journal = "International Journal of Molecular Sciences",
title = "Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)",
number = "24",
volume = "24",
doi = "10.3390/ijms242417207",
pages = "17207"
}

Popržen, T., Nikolić, I., Krstić-Milošević, D., Uzelac, B., Trifunović-Momčilov, M., Marković, M.,& Radulović, O.. (2023). Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.). in International Journal of Molecular Sciences
Basel : MDPI., 24(24), 17207.
https://doi.org/10.3390/ijms242417207

Popržen T, Nikolić I, Krstić-Milošević D, Uzelac B, Trifunović-Momčilov M, Marković M, Radulović O. Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.). in International Journal of Molecular Sciences. 2023;24(24):17207.
doi:10.3390/ijms242417207 .

Popržen, Tatjana, Nikolić, Ivan, Krstić-Milošević, Dijana, Uzelac, Branka, Trifunović-Momčilov, Milana, Marković, Marija, Radulović, Olga, "Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)" in International Journal of Molecular Sciences, 24, no. 24 (2023):17207,
https://doi.org/10.3390/ijms242417207 . .

TY  - CONF
AU  - Radulović, Olga
AU  - Popržen, Tatjana
AU  - Marković, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6193
AB  - Cilj ovog rada je bila analiza rizosfere sočivice kao mesta aktivne
produkcije auksina bakterijskog porekla, primarno indol-3-acetatne kiseline (IAA). Produkcija IAA je testirana na 21 različitom bakterijskom soju. Četiri bakterijska soja: Pseudomonas oryzihabitans 15, Pseudomonas putida 23, Hafnia paralvei 43 i Pseudomonas yamanorum 47 odabrana su na osnovu povećane produkcije IAA u odnosu na ostale testirane sojeve. Ovi sojevi su pokazali neprekinutu produkciju IAA i pod nepovoljnim uslovima: u kiseloj i baznoj sredini, kao i u hipersalinoj sredini. Naši rezultati sugerišu da ovi sojevi mogu imati biotehnološku primenu u zaštiti biljaka i povećanju prinosa putem produkcije auksina kao centralnih regulatora rasta i razvića biljaka.
AB  - The aim of this study was to analyze the rhizosphere of duckweed as a site of
active production of auxins of bacterial origin, primarily indole-3-acetic acid (IAA). IAA production was tested on 21 different bacterial strains. Four bacterial strains: Pseudomonas oryzihabitans 15, Pseudomonas putida 23, Hafnia paralvei 43 and Pseudomonas yamanorum 47 were selected based on increased IAA production compared to other strains tested. These strains showed continuous production of IAA even under unfavorable conditions: in acidic and basic environments, as well as in hypersaline environments. Our results suggest that these strains may have biotechnological applications in plant protection and increase yields through auxin production as central regulators of plant growth and development.
PB  - Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu
C3  - Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia
T1  - Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla
T1  - Potential of the rhizosphere of Duckweed (Lemna minor L.) for the production of auxins of bacterial origin
DO  - 10.46793/SBT27.401R
SP  - 401
EP  - 406
ER  - 

@conference{
author = "Radulović, Olga and Popržen, Tatjana and Marković, Marija",
year = "2022",
abstract = "Cilj ovog rada je bila analiza rizosfere sočivice kao mesta aktivne
produkcije auksina bakterijskog porekla, primarno indol-3-acetatne kiseline (IAA). Produkcija IAA je testirana na 21 različitom bakterijskom soju. Četiri bakterijska soja: Pseudomonas oryzihabitans 15, Pseudomonas putida 23, Hafnia paralvei 43 i Pseudomonas yamanorum 47 odabrana su na osnovu povećane produkcije IAA u odnosu na ostale testirane sojeve. Ovi sojevi su pokazali neprekinutu produkciju IAA i pod nepovoljnim uslovima: u kiseloj i baznoj sredini, kao i u hipersalinoj sredini. Naši rezultati sugerišu da ovi sojevi mogu imati biotehnološku primenu u zaštiti biljaka i povećanju prinosa putem produkcije auksina kao centralnih regulatora rasta i razvića biljaka., The aim of this study was to analyze the rhizosphere of duckweed as a site of
active production of auxins of bacterial origin, primarily indole-3-acetic acid (IAA). IAA production was tested on 21 different bacterial strains. Four bacterial strains: Pseudomonas oryzihabitans 15, Pseudomonas putida 23, Hafnia paralvei 43 and Pseudomonas yamanorum 47 were selected based on increased IAA production compared to other strains tested. These strains showed continuous production of IAA even under unfavorable conditions: in acidic and basic environments, as well as in hypersaline environments. Our results suggest that these strains may have biotechnological applications in plant protection and increase yields through auxin production as central regulators of plant growth and development.",
publisher = "Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu",
journal = "Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia",
title = "Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla, Potential of the rhizosphere of Duckweed (Lemna minor L.) for the production of auxins of bacterial origin",
doi = "10.46793/SBT27.401R",
pages = "401-406"
}

Radulović, O., Popržen, T.,& Marković, M.. (2022). Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla. in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia
Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu., 401-406.
https://doi.org/10.46793/SBT27.401R

Radulović O, Popržen T, Marković M. Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla. in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia. 2022;:401-406.
doi:10.46793/SBT27.401R .

Radulović, Olga, Popržen, Tatjana, Marković, Marija, "Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla" in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia (2022):401-406,
https://doi.org/10.46793/SBT27.401R . .

TY  - CONF
AU  - Radulović, Olga
AU  - Popržen, Tatjana
AU  - Marković, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6195
AB  - Modern agriculture relies heavily on the use of synthetic herbicides: however, although efficient, these chemicals are also harmful to the environment. Therefore, there is an ever-increasing need for alternative herbicides: ethanol and indole-3-acetic acid (IAA) are among the candidates. Ethanol can be cheaply produced from a vast variety of substrates in large-scale fermentation processes. Moreover, in the last 2 decades, its applicability as an alternative, biosustainable herbicide is also being recognized. Ethanol has a well-known phytotoxic effect, although its mechanisms of actions are still poorly understood. IAA, a plant phytohormone, exerts its negative effect when applied in higher doses. Each plant has different sensitivity to ethanol and IAA. This property can be used to suppress the growth of harmful species (e.g. weeds) while stimulating the growth of desired cultures. In this study, we tested the effects of IAA and ethanol on duckweeds (Lemna minor L.). We measured non-enzymatic parameters of oxidative stress and multiplication rates of duckweeds exposed to IAA and to ethanol, respectively. Our results showed that higher concen-trations of IAA (>1 mg l-1) suppress the growth of duckweeds while even small amounts of ethanol (0.5% and 1% v/v) were sufficient to reduce the growth and biomass production of duckweeds. In conclusion, ethanol and IAA can exert significant negative effects on plant growth, at least in part by increasing oxidative stress in plants, and can therefore be potentially used as low-cost, eco-friendly herbicides.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth
SP  - 100
EP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6195
ER  - 

@conference{
author = "Radulović, Olga and Popržen, Tatjana and Marković, Marija",
year = "2022",
abstract = "Modern agriculture relies heavily on the use of synthetic herbicides: however, although efficient, these chemicals are also harmful to the environment. Therefore, there is an ever-increasing need for alternative herbicides: ethanol and indole-3-acetic acid (IAA) are among the candidates. Ethanol can be cheaply produced from a vast variety of substrates in large-scale fermentation processes. Moreover, in the last 2 decades, its applicability as an alternative, biosustainable herbicide is also being recognized. Ethanol has a well-known phytotoxic effect, although its mechanisms of actions are still poorly understood. IAA, a plant phytohormone, exerts its negative effect when applied in higher doses. Each plant has different sensitivity to ethanol and IAA. This property can be used to suppress the growth of harmful species (e.g. weeds) while stimulating the growth of desired cultures. In this study, we tested the effects of IAA and ethanol on duckweeds (Lemna minor L.). We measured non-enzymatic parameters of oxidative stress and multiplication rates of duckweeds exposed to IAA and to ethanol, respectively. Our results showed that higher concen-trations of IAA (>1 mg l-1) suppress the growth of duckweeds while even small amounts of ethanol (0.5% and 1% v/v) were sufficient to reduce the growth and biomass production of duckweeds. In conclusion, ethanol and IAA can exert significant negative effects on plant growth, at least in part by increasing oxidative stress in plants, and can therefore be potentially used as low-cost, eco-friendly herbicides.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth",
pages = "100-100",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6195"
}

Radulović, O., Popržen, T.,& Marković, M.. (2022). Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 100-100.
https://hdl.handle.net/21.15107/rcub_ibiss_6195

Radulović O, Popržen T, Marković M. Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:100-100.
https://hdl.handle.net/21.15107/rcub_ibiss_6195 .

Radulović, Olga, Popržen, Tatjana, Marković, Marija, "Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):100-100,
https://hdl.handle.net/21.15107/rcub_ibiss_6195 .