TY  - JOUR
AU  - Pejin, Boris
AU  - Stošić-Grujičić, Stanislava
AU  - Bogdanović, Gordana M
AU  - Hegedis, Aleksandar E
AU  - Karaman, Ivo M
AU  - Stojanović, Ivana D.
AU  - Nikolić, Ivana
AU  - Kojić, Vesna V
AU  - Horvatović, Mladen
AU  - Radotić, Ksenija
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1060
AB  - The immunomodulatory and anticarcinogenic activity of the freshwater bryozoan Hyalinella punctata methanolic extract (MEx) was evaluated in vitro on selected biosystems for the first time. Murine lymphocytes and macrophages were used for testing of MEx effects on cell proliferation and nitric oxide (NO) production, respectively, while human cancer cell lines were utilized for measuring its activity against cancer cells. The results suggest a strong and tissue-specific immunomodifying activity (IC50 values for inhibition of proliferation of lymph node and spleen-derived lymphocytes were 4.1 mu g/ml and 9.7 mu g/ml, respectively) and moderate anticancer activity of MEx (IC50 value for the MCF-7 cell line was 24.13 mu g/ml). Down-regulation of macrophage NO production was also obtained. The potential use of H. punctata-derived natural products for the treatment of human chronic inflammatory diseases and cancer is worthy of further investigation.
T2  - Digest Journal of Nanomaterials and Biostructures
T1  - In Vitro Evaluation of the Immunomodulatory and Anticarcinogenic Activity of the Freshwater Bryozoan Hyalinella Punctata Methanolic Extract
IS  - 1
VL  - 8
SP  - 301
EP  - 195
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1060
ER  - 

@article{
author = "Pejin, Boris and Stošić-Grujičić, Stanislava and Bogdanović, Gordana M and Hegedis, Aleksandar E and Karaman, Ivo M and Stojanović, Ivana D. and Nikolić, Ivana and Kojić, Vesna V and Horvatović, Mladen and Radotić, Ksenija",
year = "2013",
abstract = "The immunomodulatory and anticarcinogenic activity of the freshwater bryozoan Hyalinella punctata methanolic extract (MEx) was evaluated in vitro on selected biosystems for the first time. Murine lymphocytes and macrophages were used for testing of MEx effects on cell proliferation and nitric oxide (NO) production, respectively, while human cancer cell lines were utilized for measuring its activity against cancer cells. The results suggest a strong and tissue-specific immunomodifying activity (IC50 values for inhibition of proliferation of lymph node and spleen-derived lymphocytes were 4.1 mu g/ml and 9.7 mu g/ml, respectively) and moderate anticancer activity of MEx (IC50 value for the MCF-7 cell line was 24.13 mu g/ml). Down-regulation of macrophage NO production was also obtained. The potential use of H. punctata-derived natural products for the treatment of human chronic inflammatory diseases and cancer is worthy of further investigation.",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "In Vitro Evaluation of the Immunomodulatory and Anticarcinogenic Activity of the Freshwater Bryozoan Hyalinella Punctata Methanolic Extract",
number = "1",
volume = "8",
pages = "301-195",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1060"
}

Pejin, B., Stošić-Grujičić, S., Bogdanović, G. M., Hegedis, A. E., Karaman, I. M., Stojanović, I. D., Nikolić, I., Kojić, V. V., Horvatović, M.,& Radotić, K.. (2013). In Vitro Evaluation of the Immunomodulatory and Anticarcinogenic Activity of the Freshwater Bryozoan Hyalinella Punctata Methanolic Extract. in Digest Journal of Nanomaterials and Biostructures, 8(1), 301-195.
https://hdl.handle.net/21.15107/rcub_ibiss_1060

Pejin B, Stošić-Grujičić S, Bogdanović GM, Hegedis AE, Karaman IM, Stojanović ID, Nikolić I, Kojić VV, Horvatović M, Radotić K. In Vitro Evaluation of the Immunomodulatory and Anticarcinogenic Activity of the Freshwater Bryozoan Hyalinella Punctata Methanolic Extract. in Digest Journal of Nanomaterials and Biostructures. 2013;8(1):301-195.
https://hdl.handle.net/21.15107/rcub_ibiss_1060 .

Pejin, Boris, Stošić-Grujičić, Stanislava, Bogdanović, Gordana M, Hegedis, Aleksandar E, Karaman, Ivo M, Stojanović, Ivana D., Nikolić, Ivana, Kojić, Vesna V, Horvatović, Mladen, Radotić, Ksenija, "In Vitro Evaluation of the Immunomodulatory and Anticarcinogenic Activity of the Freshwater Bryozoan Hyalinella Punctata Methanolic Extract" in Digest Journal of Nanomaterials and Biostructures, 8, no. 1 (2013):301-195,
https://hdl.handle.net/21.15107/rcub_ibiss_1060 .

TY  - JOUR
AU  - Stankov, Karmen
AU  - Bogdanović, Gordana M
AU  - Stankov, Suncica
AU  - Drasković, Dragan O
AU  - Grubor-Lajšić, Gordana
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1187
AB  - Purpose: Activation of T cells by direct stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin (Io) results in numerous downstream signals that activate pathways enabling T cells to proliferate and produce cytokines. Inducible T cell activation is regulated predominantly at the transcriptional level. Therefore, we were interested to analyze the transcriptional activity of the 19 genes involved in the regulation of several important cellular processes. Methods: Quantitative real-time (RT) PCR analysis was performed using mRNA-specific primers and SybrGreen for relative mRNA expression levels of all the examined genes. Results: Our results showed c-kit expression in Jurkat cells, further confirmed by sequencing of c-kit mRNA-specific PCR product. The expected increased expression of interleukin (IL)-2 mRNA, together with moderate Ki-67 upregulation, indicate the proliferation of PMA/Io treated Jurkat cells. Significant upregulation of nuclear factor (NF)-kappa B, JNK and the prosurvival Bcl-2 was followed by activation of only one protein kinase RNA-like endoplasmic reticulum kinase (PERK) out of 3 main endoplasmic reticulum (ER) stress subpathways (ATF6 and spliced XBP were downregulated). NF-kappa B and JNK activation, as well as ERK down regulation were reactive oxygen species (ROS)-independent, shown by the lack of activation of antioxidative enzymes (SOD, NOS, GSTP 1, gGCS and GR). C-kit was downregulated in the absence of exogenous SCF (c-kit ligand). Conclusion: Based on these data it is concluded that the PMA/Io treatment of Jurkat cells induced increased expression of IL-2. followed by upregulation of prosurvival genes belonging to the Bcl-2 family. Neither c-kit nor the antioxidative system were activated, excluding their role in Jurkat T-cell activation in the absence of exogenous c-kit ligand SCF.
T2  - Journal of Buon
T1  - Expression analysis of genes involved in apoptosis, proliferation and endoplasmic reticulum stress in ionomycin/PMA treated Jurkat cells
IS  - 2
VL  - 17
EP  - 376
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1187
ER  - 

@article{
author = "Stankov, Karmen and Bogdanović, Gordana M and Stankov, Suncica and Drasković, Dragan O and Grubor-Lajšić, Gordana and Spasić, Mihajlo and Blagojević, Duško",
year = "2012",
abstract = "Purpose: Activation of T cells by direct stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin (Io) results in numerous downstream signals that activate pathways enabling T cells to proliferate and produce cytokines. Inducible T cell activation is regulated predominantly at the transcriptional level. Therefore, we were interested to analyze the transcriptional activity of the 19 genes involved in the regulation of several important cellular processes. Methods: Quantitative real-time (RT) PCR analysis was performed using mRNA-specific primers and SybrGreen for relative mRNA expression levels of all the examined genes. Results: Our results showed c-kit expression in Jurkat cells, further confirmed by sequencing of c-kit mRNA-specific PCR product. The expected increased expression of interleukin (IL)-2 mRNA, together with moderate Ki-67 upregulation, indicate the proliferation of PMA/Io treated Jurkat cells. Significant upregulation of nuclear factor (NF)-kappa B, JNK and the prosurvival Bcl-2 was followed by activation of only one protein kinase RNA-like endoplasmic reticulum kinase (PERK) out of 3 main endoplasmic reticulum (ER) stress subpathways (ATF6 and spliced XBP were downregulated). NF-kappa B and JNK activation, as well as ERK down regulation were reactive oxygen species (ROS)-independent, shown by the lack of activation of antioxidative enzymes (SOD, NOS, GSTP 1, gGCS and GR). C-kit was downregulated in the absence of exogenous SCF (c-kit ligand). Conclusion: Based on these data it is concluded that the PMA/Io treatment of Jurkat cells induced increased expression of IL-2. followed by upregulation of prosurvival genes belonging to the Bcl-2 family. Neither c-kit nor the antioxidative system were activated, excluding their role in Jurkat T-cell activation in the absence of exogenous c-kit ligand SCF.",
journal = "Journal of Buon",
title = "Expression analysis of genes involved in apoptosis, proliferation and endoplasmic reticulum stress in ionomycin/PMA treated Jurkat cells",
number = "2",
volume = "17",
pages = "376",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1187"
}

Stankov, K., Bogdanović, G. M., Stankov, S., Drasković, D. O., Grubor-Lajšić, G., Spasić, M.,& Blagojević, D.. (2012). Expression analysis of genes involved in apoptosis, proliferation and endoplasmic reticulum stress in ionomycin/PMA treated Jurkat cells. in Journal of Buon, 17(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1187

Stankov K, Bogdanović GM, Stankov S, Drasković DO, Grubor-Lajšić G, Spasić M, Blagojević D. Expression analysis of genes involved in apoptosis, proliferation and endoplasmic reticulum stress in ionomycin/PMA treated Jurkat cells. in Journal of Buon. 2012;17(2):null-376.
https://hdl.handle.net/21.15107/rcub_ibiss_1187 .

Stankov, Karmen, Bogdanović, Gordana M, Stankov, Suncica, Drasković, Dragan O, Grubor-Lajšić, Gordana, Spasić, Mihajlo, Blagojević, Duško, "Expression analysis of genes involved in apoptosis, proliferation and endoplasmic reticulum stress in ionomycin/PMA treated Jurkat cells" in Journal of Buon, 17, no. 2 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1187 .

TY  - CONF
AU  - Canadanović-Brunet, Jasna M
AU  - Bogdanović, Gordana M
AU  - Andrić, Nebojsa L
AU  - Stanić, Bojana Đ
AU  - Spasić, Mihajlo
AU  - Jovanović, Tanja P
AU  - Baltić, Vladimir V
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1370
C3  - Ejc Supplements
T1  - The effects of proanthocyanidins on cardiotoxic and antitumour activity of doxorubicin
IS  - 5
VL  - 8
EP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1370
ER  - 

@conference{
author = "Canadanović-Brunet, Jasna M and Bogdanović, Gordana M and Andrić, Nebojsa L and Stanić, Bojana Đ and Spasić, Mihajlo and Jovanović, Tanja P and Baltić, Vladimir V",
year = "2010",
journal = "Ejc Supplements",
title = "The effects of proanthocyanidins on cardiotoxic and antitumour activity of doxorubicin",
number = "5",
volume = "8",
pages = "63",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1370"
}

Canadanović-Brunet, J. M., Bogdanović, G. M., Andrić, N. L., Stanić, B. Đ., Spasić, M., Jovanović, T. P.,& Baltić, V. V.. (2010). The effects of proanthocyanidins on cardiotoxic and antitumour activity of doxorubicin. in Ejc Supplements, 8(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1370

Canadanović-Brunet JM, Bogdanović GM, Andrić NL, Stanić BĐ, Spasić M, Jovanović TP, Baltić VV. The effects of proanthocyanidins on cardiotoxic and antitumour activity of doxorubicin. in Ejc Supplements. 2010;8(5):null-63.
https://hdl.handle.net/21.15107/rcub_ibiss_1370 .

Canadanović-Brunet, Jasna M, Bogdanović, Gordana M, Andrić, Nebojsa L, Stanić, Bojana Đ, Spasić, Mihajlo, Jovanović, Tanja P, Baltić, Vladimir V, "The effects of proanthocyanidins on cardiotoxic and antitumour activity of doxorubicin" in Ejc Supplements, 8, no. 5 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1370 .

TY  - JOUR
AU  - Bogdanović, Visnja V
AU  - Stankov, Karmen
AU  - Icević, Ivana D
AU  - Zikić, Dragan R
AU  - Nikolić, Aleksandra L.
AU  - Solajić, Slavica V
AU  - Đorđević, Aleksandar N
AU  - Bogdanović, Gordana M
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1532
AB  - Radiotherapy-induced toxicity is a major dose-limiting factor in anti-cancer treatment. Ionizing radiation leads to the formation of reactive oxygen and nitrogen species (ROS/RNS) that are associated with radiation-induced cell death. Investigations of biological effects of fullerenol have provided evidence for its ROS/RNS scavenger properties in vitro and radioprotective efficiency in vivo. Therefore we were interested to evaluate its radioprotective properties in vitro in the human erythroleukemia cell line. Pretreatment of irradiated cells by fullerenol exerted statistically significant effects on cell numbers and the response of antioxidative enzymes to X-ray irradiation-induced oxidative stress in cells. Our study provides evidence that the pre-treatment with fullerenol enhanced the enzymatic activity of superoxide dismulase and glutathione peroxidase in irradiated K562 cells.
T2  - Journal of Radiation Research
T1  - Fullerenol C-60(OH)(24) effects on antioxidative enzymes activity in irradiated human erythroleukemia cell line
IS  - 3
VL  - 49
EP  - 327
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1532
ER  - 

@article{
author = "Bogdanović, Visnja V and Stankov, Karmen and Icević, Ivana D and Zikić, Dragan R and Nikolić, Aleksandra L. and Solajić, Slavica V and Đorđević, Aleksandar N and Bogdanović, Gordana M",
year = "2008",
abstract = "Radiotherapy-induced toxicity is a major dose-limiting factor in anti-cancer treatment. Ionizing radiation leads to the formation of reactive oxygen and nitrogen species (ROS/RNS) that are associated with radiation-induced cell death. Investigations of biological effects of fullerenol have provided evidence for its ROS/RNS scavenger properties in vitro and radioprotective efficiency in vivo. Therefore we were interested to evaluate its radioprotective properties in vitro in the human erythroleukemia cell line. Pretreatment of irradiated cells by fullerenol exerted statistically significant effects on cell numbers and the response of antioxidative enzymes to X-ray irradiation-induced oxidative stress in cells. Our study provides evidence that the pre-treatment with fullerenol enhanced the enzymatic activity of superoxide dismulase and glutathione peroxidase in irradiated K562 cells.",
journal = "Journal of Radiation Research",
title = "Fullerenol C-60(OH)(24) effects on antioxidative enzymes activity in irradiated human erythroleukemia cell line",
number = "3",
volume = "49",
pages = "327",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1532"
}

Bogdanović, V. V., Stankov, K., Icević, I. D., Zikić, D. R., Nikolić, A. L., Solajić, S. V., Đorđević, A. N.,& Bogdanović, G. M.. (2008). Fullerenol C-60(OH)(24) effects on antioxidative enzymes activity in irradiated human erythroleukemia cell line. in Journal of Radiation Research, 49(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1532

Bogdanović VV, Stankov K, Icević ID, Zikić DR, Nikolić AL, Solajić SV, Đorđević AN, Bogdanović GM. Fullerenol C-60(OH)(24) effects on antioxidative enzymes activity in irradiated human erythroleukemia cell line. in Journal of Radiation Research. 2008;49(3):null-327.
https://hdl.handle.net/21.15107/rcub_ibiss_1532 .

Bogdanović, Visnja V, Stankov, Karmen, Icević, Ivana D, Zikić, Dragan R, Nikolić, Aleksandra L., Solajić, Slavica V, Đorđević, Aleksandar N, Bogdanović, Gordana M, "Fullerenol C-60(OH)(24) effects on antioxidative enzymes activity in irradiated human erythroleukemia cell line" in Journal of Radiation Research, 49, no. 3 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1532 .