TY  - CONF
AU  - Jovanović, Aleksandra
AU  - Ćujić, Danica
AU  - Gnjatović, Marija
AU  - Stepanović, Sandra
AU  - Dinić, Svetlana
AU  - Mihailović, Mirjana
AU  - Bugarski, Branko
PY  - 2022
UR  - https://icatsconf.org/ICAT22/van
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5256
AB  - In the present research, the storage and UV stability of small unilamellar liposomes (SUVs) with encapsulated
silymarin and silibinin were investigated in terms of changes in vesicle size, polydispersity index (PDI), zeta
potential, mobility, and conductivity. Silymarin and silibinin were encapsulated within phospholipid liposomes
produced using the proliposome method and subsequently sonicated. On the 1st day, vesicle size was 1681.0±55.5
nm for silymarin liposomes and 1884.7±2.5 nm for silibinin liposomes, whereas PDI was 0.321±0.012 and
0.319±0.011. Additionally, the zeta potential of SUVs with silymarin and silibinin was -38.6±0.8 and -37.6±1.2
mV, respectively, whereas mobility and conductivity were -3.03±0.06 μmcm/Vs and 0.149±0.002 mS/cm (for
silymarin sample) and -2.94±0.09 μmcm/Vs and 0.154±0.002 mS/cm (for silibinin sample). After 28 days of storage
at 4°C, there was a statistically significant increase in the vesicle size of SUVs with silymarin and silibinin
(2703.5±44.5 and 2172.5±26.2 nm) and a decrease in zeta potential (-16.7±0.5 and -9.8±0.1 mV), mobility (-
1.31±0.04 and -0.76±0.01 μmcm/Vs), and conductivity (0.027±0.001 and 0.023±0.001 mS/cm). On the other hand,
UV irradiation did not affect changes in vesicle size and PDI of all liposomes, but it caused a decrease in zeta
potential, -32.9±1.0 mV for silymarin and -33.9±0.3 mV for silibinin, mobility, -2.64±0.1 and -2.47±0.2 μmcm/Vs,
and conductivity, 0.131±0.005 and 0.075±0.006 mS/cm. Also, after 28 days of storage, UV-irradiated SUVs with
silymarin and silibinin have significantly higher vesicle size and lower zeta potential, mobility, and conductivity in
comparison to the measurements immediately after UV irradiation. In conclusion, both non-treated and UVirradiated
silymarin- and silibinin-loaded SUVs were unstable during storage at 4°C, resulting in changes in vesicle
size, zeta potential, mobility, and conductivity, thus additional experiments for improving liposomal stability should
be performed.
PB  - Çumra, Turkey: Plusbase Akademi Publishing
C3  - Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey
T1  - Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin
SP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5256
ER  - 

@conference{
author = "Jovanović, Aleksandra and Ćujić, Danica and Gnjatović, Marija and Stepanović, Sandra and Dinić, Svetlana and Mihailović, Mirjana and Bugarski, Branko",
year = "2022",
abstract = "In the present research, the storage and UV stability of small unilamellar liposomes (SUVs) with encapsulated
silymarin and silibinin were investigated in terms of changes in vesicle size, polydispersity index (PDI), zeta
potential, mobility, and conductivity. Silymarin and silibinin were encapsulated within phospholipid liposomes
produced using the proliposome method and subsequently sonicated. On the 1st day, vesicle size was 1681.0±55.5
nm for silymarin liposomes and 1884.7±2.5 nm for silibinin liposomes, whereas PDI was 0.321±0.012 and
0.319±0.011. Additionally, the zeta potential of SUVs with silymarin and silibinin was -38.6±0.8 and -37.6±1.2
mV, respectively, whereas mobility and conductivity were -3.03±0.06 μmcm/Vs and 0.149±0.002 mS/cm (for
silymarin sample) and -2.94±0.09 μmcm/Vs and 0.154±0.002 mS/cm (for silibinin sample). After 28 days of storage
at 4°C, there was a statistically significant increase in the vesicle size of SUVs with silymarin and silibinin
(2703.5±44.5 and 2172.5±26.2 nm) and a decrease in zeta potential (-16.7±0.5 and -9.8±0.1 mV), mobility (-
1.31±0.04 and -0.76±0.01 μmcm/Vs), and conductivity (0.027±0.001 and 0.023±0.001 mS/cm). On the other hand,
UV irradiation did not affect changes in vesicle size and PDI of all liposomes, but it caused a decrease in zeta
potential, -32.9±1.0 mV for silymarin and -33.9±0.3 mV for silibinin, mobility, -2.64±0.1 and -2.47±0.2 μmcm/Vs,
and conductivity, 0.131±0.005 and 0.075±0.006 mS/cm. Also, after 28 days of storage, UV-irradiated SUVs with
silymarin and silibinin have significantly higher vesicle size and lower zeta potential, mobility, and conductivity in
comparison to the measurements immediately after UV irradiation. In conclusion, both non-treated and UVirradiated
silymarin- and silibinin-loaded SUVs were unstable during storage at 4°C, resulting in changes in vesicle
size, zeta potential, mobility, and conductivity, thus additional experiments for improving liposomal stability should
be performed.",
publisher = "Çumra, Turkey: Plusbase Akademi Publishing",
journal = "Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey",
title = "Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5256"
}

Jovanović, A., Ćujić, D., Gnjatović, M., Stepanović, S., Dinić, S., Mihailović, M.,& Bugarski, B.. (2022). Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin. in Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey
Çumra, Turkey: Plusbase Akademi Publishing., 158.
https://hdl.handle.net/21.15107/rcub_ibiss_5256

Jovanović A, Ćujić D, Gnjatović M, Stepanović S, Dinić S, Mihailović M, Bugarski B. Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin. in Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey. 2022;:158.
https://hdl.handle.net/21.15107/rcub_ibiss_5256 .

Jovanović, Aleksandra, Ćujić, Danica, Gnjatović, Marija, Stepanović, Sandra, Dinić, Svetlana, Mihailović, Mirjana, Bugarski, Branko, "Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin" in Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey (2022):158,
https://hdl.handle.net/21.15107/rcub_ibiss_5256 .