TY  - JOUR
AU  - Pavlović, Suzana
AU  - Savić, Jelena
AU  - Milojević, Jelena
AU  - Vinterhalter, Branka
AU  - Girek, Zdenka
AU  - Adžić, Slađan
AU  - Zečević, Bogoljub
AU  - Banjac, Nevena
PY  - 2020
UR  - https://doi.org/10.1007/s11240-020-01948-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4011
AB  - Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops. Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules that play important roles in regulating innate immune responses in plants. Based on involvement of tobacco MAP kinase kinase kinase (NPK1) in stress response, the effect of the expression of NPK1 transgene to NaCl salt stress tolerance in cauliflower KFRM4 lines was studied. The Agrobacterium tumefaciens-mediated transformation protocol, using EHA101(pSHX004) vector harbouring the NPK1 and phosphinothricin N-acetyltransferase (bar) genes, the cyclic somatic embryogenesis regeneration pathway, the application of acetosyringone (AS) during co-cultivation and a delayed phosphinothricine (PPT) selection procedure provided sufficient transformation efficiency of 7.33% without escapes. PCR analysis indicated the integration of both NPK1 and bar transgenes in regenerated cauliflower lines. Transgenic cauliflower lines, exposed to NaCl stress in vitro, showed higher growth rates, greater ability to retain chlorophyll and carotenoids, and increased osmotic regulation capacity compared with non-transformed control plants. The tolerance level of transformed lines correlated with the level of NPK1 gene expression estimated by RT-qPCR, and the L2 line with the highest NPK1 expression displayed the greatest tolerance to NaCl stress. None of the obtained cauliflower transformed lines grown in greenhouses showed any morphological or yield differences compared with non-transformed plants. Furthermore, the expression of the bar gene facilitated the tolerance of transformed lines to the total herbicide PPT, applied at concentrations 2-3 times higher than those routinely used for weed control in the crop field. Key message The results underlined that constitutively expressing NPK1 can significantly contribute to enhanced salt stresstolerance in cauliflower, suggesting that this could be a promising basis for the creation of new stress tolerancecruciferous vegetable lines.
PB  - Springer Science and Business Media LLC
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower
IS  - 3
VL  - 143
DO  - 10.1007/s11240-020-01948-6
SP  - 635
EP  - 651
ER  - 

@article{
author = "Pavlović, Suzana and Savić, Jelena and Milojević, Jelena and Vinterhalter, Branka and Girek, Zdenka and Adžić, Slađan and Zečević, Bogoljub and Banjac, Nevena",
year = "2020",
abstract = "Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops. Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules that play important roles in regulating innate immune responses in plants. Based on involvement of tobacco MAP kinase kinase kinase (NPK1) in stress response, the effect of the expression of NPK1 transgene to NaCl salt stress tolerance in cauliflower KFRM4 lines was studied. The Agrobacterium tumefaciens-mediated transformation protocol, using EHA101(pSHX004) vector harbouring the NPK1 and phosphinothricin N-acetyltransferase (bar) genes, the cyclic somatic embryogenesis regeneration pathway, the application of acetosyringone (AS) during co-cultivation and a delayed phosphinothricine (PPT) selection procedure provided sufficient transformation efficiency of 7.33% without escapes. PCR analysis indicated the integration of both NPK1 and bar transgenes in regenerated cauliflower lines. Transgenic cauliflower lines, exposed to NaCl stress in vitro, showed higher growth rates, greater ability to retain chlorophyll and carotenoids, and increased osmotic regulation capacity compared with non-transformed control plants. The tolerance level of transformed lines correlated with the level of NPK1 gene expression estimated by RT-qPCR, and the L2 line with the highest NPK1 expression displayed the greatest tolerance to NaCl stress. None of the obtained cauliflower transformed lines grown in greenhouses showed any morphological or yield differences compared with non-transformed plants. Furthermore, the expression of the bar gene facilitated the tolerance of transformed lines to the total herbicide PPT, applied at concentrations 2-3 times higher than those routinely used for weed control in the crop field. Key message The results underlined that constitutively expressing NPK1 can significantly contribute to enhanced salt stresstolerance in cauliflower, suggesting that this could be a promising basis for the creation of new stress tolerancecruciferous vegetable lines.",
publisher = "Springer Science and Business Media LLC",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower",
number = "3",
volume = "143",
doi = "10.1007/s11240-020-01948-6",
pages = "635-651"
}

Pavlović, S., Savić, J., Milojević, J., Vinterhalter, B., Girek, Z., Adžić, S., Zečević, B.,& Banjac, N.. (2020). Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower. in Plant Cell, Tissue and Organ Culture (PCTOC)
Springer Science and Business Media LLC., 143(3), 635-651.
https://doi.org/10.1007/s11240-020-01948-6

Pavlović S, Savić J, Milojević J, Vinterhalter B, Girek Z, Adžić S, Zečević B, Banjac N. Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower. in Plant Cell, Tissue and Organ Culture (PCTOC). 2020;143(3):635-651.
doi:10.1007/s11240-020-01948-6 .

Pavlović, Suzana, Savić, Jelena, Milojević, Jelena, Vinterhalter, Branka, Girek, Zdenka, Adžić, Slađan, Zečević, Bogoljub, Banjac, Nevena, "Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower" in Plant Cell, Tissue and Organ Culture (PCTOC), 143, no. 3 (2020):635-651,
https://doi.org/10.1007/s11240-020-01948-6 . .

TY  - JOUR
AU  - Milošević, Snežana
AU  - Savić, Slađana
AU  - Stikić, Radmila
AU  - Zečević, Bogoljub
AU  - Đorđević, Radiša
AU  - Zdravković, Jasmina
PY  - 2005
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/360
AB  - Pepper and tomato plants were grown in the commercial compost with the root system divided equally into two parts (FDR technique). At the end of the vegetative and during the generative stage of development, half of the root system was exposed to drought, while the reminding part was irrigated. One PRD treatment lasted approximately 10 days and during that period the soil water content in the dry root side was reduced to 30%. After this, the treatment was reversed, allowing the previously dried compartment to be well watered and the well-watered compartment to dry down. In control plants both compartments were watered daily to the drip point throughout the experimental period. During the experimental period the following parameters were measured: plant height, leaf number, shoot DW, fruit DW and water-use efficiency (WUE). Obtained results show that as a result of the PRD treatment, the growth of whole plants was reduced, while the fruit growth was not reduced, although the WUE was increased. These results point out that by this technique it is possible to reduce the amount of irrigation water without a significant yield reduction.
AB  - Biljke paprike i paradajza su gajene u komercijalnom supstratu sa korenovim sistemom razdvojenim na dve polovine (DSK tehnika). Na kraju vegetativne i tokom generativne faze razvića polovina korenovog sistema je izlagana suši dok je druga polovina zalivana, Jedan DSK ciklus traje oko 10 dana. i u toku tog perioda vlažnost supstrata u zasušivanom delu korenovog sistema se spušta na 30% optimalnog vodnog kapaciteta. Zatim se viši inverzija, tako da se zasušivana polovina korena zaliva, a zalivana polovina zasušuje. Kod kontrolnih biljaka ceo koren je optimalno zalivan tokom celog eksperimentalnog perioda. U toku eksperimentalnog perioda obavljena su merenja visine biljaka, broja listova, suve mase izdanka, suve mase plodova i efikasnosti korišćenja vode (WUE). Rezultati merenja ovih parametara su pokazali da je kao posledica DSK tehnike došlo do redukcije rasta biljaka ali ne i plodova, dok su WUE vrednosti porasle. Ovi rezultati su pokazali da je primenom DSK tehnike moguća redukcija količine vode za zalivanje, a da se pri tome značajno ne redukuje prinos.
PB  - Beograd: Yugoslav Association of Agricultural Engineers and Technicians
T2  - Journal of Scientific Agricultural Research
T1  - Delimično sušenje korenova - novi pristup u gajenju i navodnjavanju paprike (Capsicum annuum L) i paradajza (Lycopersicon esculentum L)
T1  - Partial root drying: New approach to the growth and irrigation of pepper (Capsicum atmuum L) and tomato (Lycopersicon escitlenlutn L)
IS  - 233
VL  - 66
SP  - 69
EP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_360
ER  - 

@article{
author = "Milošević, Snežana and Savić, Slađana and Stikić, Radmila and Zečević, Bogoljub and Đorđević, Radiša and Zdravković, Jasmina",
year = "2005, 2005",
abstract = "Pepper and tomato plants were grown in the commercial compost with the root system divided equally into two parts (FDR technique). At the end of the vegetative and during the generative stage of development, half of the root system was exposed to drought, while the reminding part was irrigated. One PRD treatment lasted approximately 10 days and during that period the soil water content in the dry root side was reduced to 30%. After this, the treatment was reversed, allowing the previously dried compartment to be well watered and the well-watered compartment to dry down. In control plants both compartments were watered daily to the drip point throughout the experimental period. During the experimental period the following parameters were measured: plant height, leaf number, shoot DW, fruit DW and water-use efficiency (WUE). Obtained results show that as a result of the PRD treatment, the growth of whole plants was reduced, while the fruit growth was not reduced, although the WUE was increased. These results point out that by this technique it is possible to reduce the amount of irrigation water without a significant yield reduction., Biljke paprike i paradajza su gajene u komercijalnom supstratu sa korenovim sistemom razdvojenim na dve polovine (DSK tehnika). Na kraju vegetativne i tokom generativne faze razvića polovina korenovog sistema je izlagana suši dok je druga polovina zalivana, Jedan DSK ciklus traje oko 10 dana. i u toku tog perioda vlažnost supstrata u zasušivanom delu korenovog sistema se spušta na 30% optimalnog vodnog kapaciteta. Zatim se viši inverzija, tako da se zasušivana polovina korena zaliva, a zalivana polovina zasušuje. Kod kontrolnih biljaka ceo koren je optimalno zalivan tokom celog eksperimentalnog perioda. U toku eksperimentalnog perioda obavljena su merenja visine biljaka, broja listova, suve mase izdanka, suve mase plodova i efikasnosti korišćenja vode (WUE). Rezultati merenja ovih parametara su pokazali da je kao posledica DSK tehnike došlo do redukcije rasta biljaka ali ne i plodova, dok su WUE vrednosti porasle. Ovi rezultati su pokazali da je primenom DSK tehnike moguća redukcija količine vode za zalivanje, a da se pri tome značajno ne redukuje prinos.",
publisher = "Beograd: Yugoslav Association of Agricultural Engineers and Technicians",
journal = "Journal of Scientific Agricultural Research",
title = "Delimično sušenje korenova - novi pristup u gajenju i navodnjavanju paprike (Capsicum annuum L) i paradajza (Lycopersicon esculentum L), Partial root drying: New approach to the growth and irrigation of pepper (Capsicum atmuum L) and tomato (Lycopersicon escitlenlutn L)",
number = "233",
volume = "66",
pages = "69-73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_360"
}

Milošević, S., Savić, S., Stikić, R., Zečević, B., Đorđević, R.,& Zdravković, J.. (2005). Delimično sušenje korenova - novi pristup u gajenju i navodnjavanju paprike (Capsicum annuum L) i paradajza (Lycopersicon esculentum L). in Journal of Scientific Agricultural Research
Beograd: Yugoslav Association of Agricultural Engineers and Technicians., 66(233), 69-73.
https://hdl.handle.net/21.15107/rcub_ibiss_360

Milošević S, Savić S, Stikić R, Zečević B, Đorđević R, Zdravković J. Delimično sušenje korenova - novi pristup u gajenju i navodnjavanju paprike (Capsicum annuum L) i paradajza (Lycopersicon esculentum L). in Journal of Scientific Agricultural Research. 2005;66(233):69-73.
https://hdl.handle.net/21.15107/rcub_ibiss_360 .

Milošević, Snežana, Savić, Slađana, Stikić, Radmila, Zečević, Bogoljub, Đorđević, Radiša, Zdravković, Jasmina, "Delimično sušenje korenova - novi pristup u gajenju i navodnjavanju paprike (Capsicum annuum L) i paradajza (Lycopersicon esculentum L)" in Journal of Scientific Agricultural Research, 66, no. 233 (2005):69-73,
https://hdl.handle.net/21.15107/rcub_ibiss_360 .