TY  - CONF
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Pavlović, Suzana
AU  - Živković, Suzana
AU  - Vinterhalter, Dragan
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5412
AB  - Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.
PB  - East Sarajevo : Faculty of Agriculture
PB  - eas
C3  - Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina
T1  - Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)
SP  - 213
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5412
ER  - 

@conference{
author = "Belić, Maja and Zdravković-Korać, Snežana and Pavlović, Suzana and Živković, Suzana and Vinterhalter, Dragan and Ćalić, Dušica and Milojević, Jelena",
year = "2021",
abstract = "Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.",
publisher = "East Sarajevo : Faculty of Agriculture, eas",
journal = "Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina",
title = "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)",
pages = "213",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5412"
}

Belić, M., Zdravković-Korać, S., Pavlović, S., Živković, S., Vinterhalter, D., Ćalić, D.,& Milojević, J.. (2021). Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina
East Sarajevo : Faculty of Agriculture., 213.
https://hdl.handle.net/21.15107/rcub_ibiss_5412

Belić M, Zdravković-Korać S, Pavlović S, Živković S, Vinterhalter D, Ćalić D, Milojević J. Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina. 2021;:213.
https://hdl.handle.net/21.15107/rcub_ibiss_5412 .

Belić, Maja, Zdravković-Korać, Snežana, Pavlović, Suzana, Živković, Suzana, Vinterhalter, Dragan, Ćalić, Dušica, Milojević, Jelena, "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)" in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina (2021):213,
https://hdl.handle.net/21.15107/rcub_ibiss_5412 .

TY  - JOUR
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Uzelac, Branka
AU  - Ćalić, Dušica
AU  - Pavlović, Suzana
AU  - Milojević, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3997
AB  - High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80–100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20–60% of regenerating explants. The explants from the majority of seedlings (52–100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10–20 (43.5%) up to > 20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1–10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.
PB  - Nature Research
T2  - Scientific Reports
T1  - Variability in somatic embryo-forming capacity of spinach
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-76279-9
SP  - 19290
ER  - 

@article{
author = "Belić, Maja and Zdravković-Korać, Snežana and Uzelac, Branka and Ćalić, Dušica and Pavlović, Suzana and Milojević, Jelena",
year = "2020",
abstract = "High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80–100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20–60% of regenerating explants. The explants from the majority of seedlings (52–100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10–20 (43.5%) up to > 20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1–10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Variability in somatic embryo-forming capacity of spinach",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-76279-9",
pages = "19290"
}

Belić, M., Zdravković-Korać, S., Uzelac, B., Ćalić, D., Pavlović, S.,& Milojević, J.. (2020). Variability in somatic embryo-forming capacity of spinach. in Scientific Reports
Nature Research., 10(1), 19290.
https://doi.org/10.1038/s41598-020-76279-9

Belić M, Zdravković-Korać S, Uzelac B, Ćalić D, Pavlović S, Milojević J. Variability in somatic embryo-forming capacity of spinach. in Scientific Reports. 2020;10(1):19290.
doi:10.1038/s41598-020-76279-9 .

Belić, Maja, Zdravković-Korać, Snežana, Uzelac, Branka, Ćalić, Dušica, Pavlović, Suzana, Milojević, Jelena, "Variability in somatic embryo-forming capacity of spinach" in Scientific Reports, 10, no. 1 (2020):19290,
https://doi.org/10.1038/s41598-020-76279-9 . .

TY  - JOUR
AU  - Pavlović, Suzana
AU  - Savić, Jelena
AU  - Milojević, Jelena
AU  - Vinterhalter, Branka
AU  - Girek, Zdenka
AU  - Adžić, Slađan
AU  - Zečević, Bogoljub
AU  - Banjac, Nevena
PY  - 2020
UR  - https://doi.org/10.1007/s11240-020-01948-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4011
AB  - Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops. Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules that play important roles in regulating innate immune responses in plants. Based on involvement of tobacco MAP kinase kinase kinase (NPK1) in stress response, the effect of the expression of NPK1 transgene to NaCl salt stress tolerance in cauliflower KFRM4 lines was studied. The Agrobacterium tumefaciens-mediated transformation protocol, using EHA101(pSHX004) vector harbouring the NPK1 and phosphinothricin N-acetyltransferase (bar) genes, the cyclic somatic embryogenesis regeneration pathway, the application of acetosyringone (AS) during co-cultivation and a delayed phosphinothricine (PPT) selection procedure provided sufficient transformation efficiency of 7.33% without escapes. PCR analysis indicated the integration of both NPK1 and bar transgenes in regenerated cauliflower lines. Transgenic cauliflower lines, exposed to NaCl stress in vitro, showed higher growth rates, greater ability to retain chlorophyll and carotenoids, and increased osmotic regulation capacity compared with non-transformed control plants. The tolerance level of transformed lines correlated with the level of NPK1 gene expression estimated by RT-qPCR, and the L2 line with the highest NPK1 expression displayed the greatest tolerance to NaCl stress. None of the obtained cauliflower transformed lines grown in greenhouses showed any morphological or yield differences compared with non-transformed plants. Furthermore, the expression of the bar gene facilitated the tolerance of transformed lines to the total herbicide PPT, applied at concentrations 2-3 times higher than those routinely used for weed control in the crop field. Key message The results underlined that constitutively expressing NPK1 can significantly contribute to enhanced salt stresstolerance in cauliflower, suggesting that this could be a promising basis for the creation of new stress tolerancecruciferous vegetable lines.
PB  - Springer Science and Business Media LLC
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower
IS  - 3
VL  - 143
DO  - 10.1007/s11240-020-01948-6
SP  - 635
EP  - 651
ER  - 

@article{
author = "Pavlović, Suzana and Savić, Jelena and Milojević, Jelena and Vinterhalter, Branka and Girek, Zdenka and Adžić, Slađan and Zečević, Bogoljub and Banjac, Nevena",
year = "2020",
abstract = "Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops. Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules that play important roles in regulating innate immune responses in plants. Based on involvement of tobacco MAP kinase kinase kinase (NPK1) in stress response, the effect of the expression of NPK1 transgene to NaCl salt stress tolerance in cauliflower KFRM4 lines was studied. The Agrobacterium tumefaciens-mediated transformation protocol, using EHA101(pSHX004) vector harbouring the NPK1 and phosphinothricin N-acetyltransferase (bar) genes, the cyclic somatic embryogenesis regeneration pathway, the application of acetosyringone (AS) during co-cultivation and a delayed phosphinothricine (PPT) selection procedure provided sufficient transformation efficiency of 7.33% without escapes. PCR analysis indicated the integration of both NPK1 and bar transgenes in regenerated cauliflower lines. Transgenic cauliflower lines, exposed to NaCl stress in vitro, showed higher growth rates, greater ability to retain chlorophyll and carotenoids, and increased osmotic regulation capacity compared with non-transformed control plants. The tolerance level of transformed lines correlated with the level of NPK1 gene expression estimated by RT-qPCR, and the L2 line with the highest NPK1 expression displayed the greatest tolerance to NaCl stress. None of the obtained cauliflower transformed lines grown in greenhouses showed any morphological or yield differences compared with non-transformed plants. Furthermore, the expression of the bar gene facilitated the tolerance of transformed lines to the total herbicide PPT, applied at concentrations 2-3 times higher than those routinely used for weed control in the crop field. Key message The results underlined that constitutively expressing NPK1 can significantly contribute to enhanced salt stresstolerance in cauliflower, suggesting that this could be a promising basis for the creation of new stress tolerancecruciferous vegetable lines.",
publisher = "Springer Science and Business Media LLC",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower",
number = "3",
volume = "143",
doi = "10.1007/s11240-020-01948-6",
pages = "635-651"
}

Pavlović, S., Savić, J., Milojević, J., Vinterhalter, B., Girek, Z., Adžić, S., Zečević, B.,& Banjac, N.. (2020). Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower. in Plant Cell, Tissue and Organ Culture (PCTOC)
Springer Science and Business Media LLC., 143(3), 635-651.
https://doi.org/10.1007/s11240-020-01948-6

Pavlović S, Savić J, Milojević J, Vinterhalter B, Girek Z, Adžić S, Zečević B, Banjac N. Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower. in Plant Cell, Tissue and Organ Culture (PCTOC). 2020;143(3):635-651.
doi:10.1007/s11240-020-01948-6 .

Pavlović, Suzana, Savić, Jelena, Milojević, Jelena, Vinterhalter, Branka, Girek, Zdenka, Adžić, Slađan, Zečević, Bogoljub, Banjac, Nevena, "Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower" in Plant Cell, Tissue and Organ Culture (PCTOC), 143, no. 3 (2020):635-651,
https://doi.org/10.1007/s11240-020-01948-6 . .

TY  - JOUR
AU  - Pavlović, Suzana
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Vinterhalter, Dragan V
AU  - Zdravković, Jasmina
AU  - Cvikić, Dejan
AU  - Banjac, Nevena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1005
AB  - A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.
T2  - Plant Cell Tissue and Organ Culture
T1  - Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)
IS  - 3
VL  - 113
SP  - 31
EP  - 406
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1005
ER  - 

@article{
author = "Pavlović, Suzana and Vinterhalter, Branka and Zdravković-Korać, Snežana and Vinterhalter, Dragan V and Zdravković, Jasmina and Cvikić, Dejan and Banjac, Nevena",
year = "2013",
abstract = "A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)",
number = "3",
volume = "113",
pages = "31-406",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1005"
}

Pavlović, S., Vinterhalter, B., Zdravković-Korać, S., Vinterhalter, D. V., Zdravković, J., Cvikić, D.,& Banjac, N.. (2013). Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis). in Plant Cell Tissue and Organ Culture, 113(3), 31-406.
https://hdl.handle.net/21.15107/rcub_ibiss_1005

Pavlović S, Vinterhalter B, Zdravković-Korać S, Vinterhalter DV, Zdravković J, Cvikić D, Banjac N. Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis). in Plant Cell Tissue and Organ Culture. 2013;113(3):31-406.
https://hdl.handle.net/21.15107/rcub_ibiss_1005 .

Pavlović, Suzana, Vinterhalter, Branka, Zdravković-Korać, Snežana, Vinterhalter, Dragan V, Zdravković, Jasmina, Cvikić, Dejan, Banjac, Nevena, "Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)" in Plant Cell Tissue and Organ Culture, 113, no. 3 (2013):31-406,
https://hdl.handle.net/21.15107/rcub_ibiss_1005 .

TY  - JOUR
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana
AU  - Pavlović, Suzana
AU  - Banjac, Nevena
AU  - Ćalić, Dušica
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
PY  - 2012
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6109
AB  - As has been previously shown, the genotype at the individual plant level has a strong effect on the regeneration capacity of spinach, and this effect quite often masks the impact of other factors, including the light environment. Therefore, apical sections of the lateral roots of the same seedling (line) were exposed in parallel to both long day (LD, 16-h photoperiod) and short day (SD, 8-h photoperiod) conditions to assess the effect of photoperiod on somatic embryo (SE) regeneration. The root sections in most lines responded with significantly higher frequencies of regeneration and mean SE numbers with LD compared to roots of the same origin with SD, while 7 of 40 lines were only able to regenerate when exposed to LD. Moreover, in all lines cultured with SD, regeneration was delayed by approximately 4 weeks. Obtained SEs were further grown and multiplied, providing material for the evaluation of the effect of light intensity on regeneration capacity. Maximal regeneration capacity was recorded at 100  μmol m−2 s−1 in all lines tested. The present research confirmed the previously observed strong effect of genotype on regeneration capacity and undoubtedly showed a significantly better response in explants exposed to LD than to SD. These results could contribute to better understanding the process of SE induction in spinach and further improve the efficiency of this process.
PB  - Amsterdam: Elsevier
T2  - Scientia Horticulturae
T1  - Long days promote somatic embryogenesis in spinach
VL  - 142
DO  - 10.1016/j.scienta.2012.04.020
SP  - 32
EP  - 37
ER  - 

@article{
author = "Milojević, Jelena and Tubić, Ljiljana and Pavlović, Suzana and Banjac, Nevena and Ćalić, Dušica and Vinterhalter, Branka and Zdravković-Korać, Snežana",
year = "2012",
abstract = "As has been previously shown, the genotype at the individual plant level has a strong effect on the regeneration capacity of spinach, and this effect quite often masks the impact of other factors, including the light environment. Therefore, apical sections of the lateral roots of the same seedling (line) were exposed in parallel to both long day (LD, 16-h photoperiod) and short day (SD, 8-h photoperiod) conditions to assess the effect of photoperiod on somatic embryo (SE) regeneration. The root sections in most lines responded with significantly higher frequencies of regeneration and mean SE numbers with LD compared to roots of the same origin with SD, while 7 of 40 lines were only able to regenerate when exposed to LD. Moreover, in all lines cultured with SD, regeneration was delayed by approximately 4 weeks. Obtained SEs were further grown and multiplied, providing material for the evaluation of the effect of light intensity on regeneration capacity. Maximal regeneration capacity was recorded at 100  μmol m−2 s−1 in all lines tested. The present research confirmed the previously observed strong effect of genotype on regeneration capacity and undoubtedly showed a significantly better response in explants exposed to LD than to SD. These results could contribute to better understanding the process of SE induction in spinach and further improve the efficiency of this process.",
publisher = "Amsterdam: Elsevier",
journal = "Scientia Horticulturae",
title = "Long days promote somatic embryogenesis in spinach",
volume = "142",
doi = "10.1016/j.scienta.2012.04.020",
pages = "32-37"
}

Milojević, J., Tubić, L., Pavlović, S., Banjac, N., Ćalić, D., Vinterhalter, B.,& Zdravković-Korać, S.. (2012). Long days promote somatic embryogenesis in spinach. in Scientia Horticulturae
Amsterdam: Elsevier., 142, 32-37.
https://doi.org/10.1016/j.scienta.2012.04.020

Milojević J, Tubić L, Pavlović S, Banjac N, Ćalić D, Vinterhalter B, Zdravković-Korać S. Long days promote somatic embryogenesis in spinach. in Scientia Horticulturae. 2012;142:32-37.
doi:10.1016/j.scienta.2012.04.020 .

Milojević, Jelena, Tubić, Ljiljana, Pavlović, Suzana, Banjac, Nevena, Ćalić, Dušica, Vinterhalter, Branka, Zdravković-Korać, Snežana, "Long days promote somatic embryogenesis in spinach" in Scientia Horticulturae, 142 (2012):32-37,
https://doi.org/10.1016/j.scienta.2012.04.020 . .

TY  - JOUR
AU  - Pavlović, Suzana
AU  - Vinterhalter, Branka
AU  - Banjac, Nevena
AU  - Adžić, S.
AU  - Pavlović, N.
AU  - Zdravković, M.
AU  - Vinterhalter, D.
PY  - 2010
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/253
AB  - Brassica oleracea varieties (red cabbage, broccoli, Savoy cabbage and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root explants of 7 day-old seedlings were incubated on Murashige and Skoog's (MS) medium supplemented with 1 mg l-1 6-benzyladenine (BA) or 6-furfurylaminopurine (KIN) in combination with 0, 0.1, and 0.2 mg l-1 indole-3-butyric acid (IBA). Hypocotyls showed the best explants in almost all varieties tested with a minimum regeneration potential of 75% and producing 3.5-7.4 shoots per explant. The BA-supplemented media were optimal for both shoot regeneration and multiplication. Shoots rooted maximally (100%) on plant growth regulator-free MS medium containing 2% or 4% sucrose. Increased sucrose content improved plant acclimation in the greenhouse.
AB  - Ispitivana je sposobnost četiri varijeteta Brassica oleracea (crveni kupus, brokoli, kelj i karfiol) da regenerišu pupoljke in vitro. Eksplantati kotiledona, hipokotila i korenova, uzetih sa 7 dana starih klijanaca, su gajeni na Murashige i Skoog (MS) hranljivoj podlozi sa dodatkom 1 mg l-1 6-benziladenina (BA) ili 6- furfurilaminopurina (KIN) u kombinaciji sa 0, 0.1, i 0.2 mg l-1 indol-3-butirične kiseline (IBA). Eksplantati hipokotila su se pokazali kao najbolji za regeneraciju kod skoro svih testiranih varijeteta sa minimalnim regenerativnim potencijalom od 75% i sa produkcijom 3.5- 7.4 pupoljaka po eksplantatu. Podloge koje su sadržale BA su bile optimalne, kako za regeneraiju pupoljaka, tako i za njihovu kasniju multiplikaciju. Maksimalan procenat oživljavanja izdanaka (100%) je postignut na MS medijumu bez dodatih regulatora rastenja, a koji je sadržao 2% ili 4% saharozu. Povećan sadržaj saharoze u medijumu za ožiljavanje uticao je na poboljšanu aklimatizaciju biljaka u stakleniku.
T2  - Archives of Biological Sciences
T1  - Regeneracija pupoljaka in vitro iz eksplantata klijanaca kod povrtarskih kultura roda Brassica - crvenog kupusa, brokolija, kelja i karfiola
T1  - In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower
IS  - 2
VL  - 62
SP  - 337
EP  - 345
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_253
ER  - 

@article{
author = "Pavlović, Suzana and Vinterhalter, Branka and Banjac, Nevena and Adžić, S. and Pavlović, N. and Zdravković, M. and Vinterhalter, D.",
year = "2010, 2010",
abstract = "Brassica oleracea varieties (red cabbage, broccoli, Savoy cabbage and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root explants of 7 day-old seedlings were incubated on Murashige and Skoog's (MS) medium supplemented with 1 mg l-1 6-benzyladenine (BA) or 6-furfurylaminopurine (KIN) in combination with 0, 0.1, and 0.2 mg l-1 indole-3-butyric acid (IBA). Hypocotyls showed the best explants in almost all varieties tested with a minimum regeneration potential of 75% and producing 3.5-7.4 shoots per explant. The BA-supplemented media were optimal for both shoot regeneration and multiplication. Shoots rooted maximally (100%) on plant growth regulator-free MS medium containing 2% or 4% sucrose. Increased sucrose content improved plant acclimation in the greenhouse., Ispitivana je sposobnost četiri varijeteta Brassica oleracea (crveni kupus, brokoli, kelj i karfiol) da regenerišu pupoljke in vitro. Eksplantati kotiledona, hipokotila i korenova, uzetih sa 7 dana starih klijanaca, su gajeni na Murashige i Skoog (MS) hranljivoj podlozi sa dodatkom 1 mg l-1 6-benziladenina (BA) ili 6- furfurilaminopurina (KIN) u kombinaciji sa 0, 0.1, i 0.2 mg l-1 indol-3-butirične kiseline (IBA). Eksplantati hipokotila su se pokazali kao najbolji za regeneraciju kod skoro svih testiranih varijeteta sa minimalnim regenerativnim potencijalom od 75% i sa produkcijom 3.5- 7.4 pupoljaka po eksplantatu. Podloge koje su sadržale BA su bile optimalne, kako za regeneraiju pupoljaka, tako i za njihovu kasniju multiplikaciju. Maksimalan procenat oživljavanja izdanaka (100%) je postignut na MS medijumu bez dodatih regulatora rastenja, a koji je sadržao 2% ili 4% saharozu. Povećan sadržaj saharoze u medijumu za ožiljavanje uticao je na poboljšanu aklimatizaciju biljaka u stakleniku.",
journal = "Archives of Biological Sciences",
title = "Regeneracija pupoljaka in vitro iz eksplantata klijanaca kod povrtarskih kultura roda Brassica - crvenog kupusa, brokolija, kelja i karfiola, In vitro shoot regeneration from seedling explants in Brassica vegetables: Red cabbage, broccoli, Savoy cabbage and cauliflower",
number = "2",
volume = "62",
pages = "337-345",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_253"
}

Pavlović, S., Vinterhalter, B., Banjac, N., Adžić, S., Pavlović, N., Zdravković, M.,& Vinterhalter, D.. (2010). Regeneracija pupoljaka in vitro iz eksplantata klijanaca kod povrtarskih kultura roda Brassica - crvenog kupusa, brokolija, kelja i karfiola. in Archives of Biological Sciences, 62(2), 337-345.
https://hdl.handle.net/21.15107/rcub_ibiss_253

Pavlović S, Vinterhalter B, Banjac N, Adžić S, Pavlović N, Zdravković M, Vinterhalter D. Regeneracija pupoljaka in vitro iz eksplantata klijanaca kod povrtarskih kultura roda Brassica - crvenog kupusa, brokolija, kelja i karfiola. in Archives of Biological Sciences. 2010;62(2):337-345.
https://hdl.handle.net/21.15107/rcub_ibiss_253 .

Pavlović, Suzana, Vinterhalter, Branka, Banjac, Nevena, Adžić, S., Pavlović, N., Zdravković, M., Vinterhalter, D., "Regeneracija pupoljaka in vitro iz eksplantata klijanaca kod povrtarskih kultura roda Brassica - crvenog kupusa, brokolija, kelja i karfiola" in Archives of Biological Sciences, 62, no. 2 (2010):337-345,
https://hdl.handle.net/21.15107/rcub_ibiss_253 .