TY  - JOUR
AU  - Petrović, Tamara
AU  - Vučić, Tijana
AU  - Burraco, Pablo
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Radovanović, Tijana
AU  - Šajkunić, Sanja
AU  - Ivanović, Ana
AU  - Prokić, Marko
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0306456523000153
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5463
AB  - Ectotherms are particularly sensitive to global warming due to their limited capacity to thermoregulate, which can impact their performance and fitness. From a physiological standpoint, higher temperatures often enhance biological processes that can induce the production of reactive oxygen species and result in a state of cellular oxidative stress. Temperature alters interspecific interactions, including species hybridization. Hybridization under different thermal conditions could amplify parental (genetic) incompatibilities, thus affecting a hybrid's development and distribution. Understanding the impact of global warming on the physiology of hybrids and particularly their oxidative status could help in predicting future scenarios in ecosystems and in hybrids. In the present study, we investigated the effect of water temperature on the development, growth and oxidative stress of two crested newt species and their reciprocal hybrids. Larvae of Triturus macedonicus and T. ivanbureschi, and their T. macedonicus-mothered and T. ivanbureschi-mothered hybrids were exposed for 30 days to temperatures of 19°C and 24°C. Under the higher temperature, the hybrids experienced increases in both growth and developmental rates, while parental species exhibited accelerated growth (T. macedonicus) or development (T. ivanbureschi). Warm conditions also had different effects on the oxidative status of hybrid and parental species. Parental species had enhanced antioxidant responses (catalase, glutathione peroxidase, glutathione S-transferase and SH groups), which allowed them to alleviate temperature-induced stress (revealed by the absence of oxidative damage). However, warming induced an antioxidant response in the hybrids, including oxidative damage in the form of lipid peroxidation. These findings point to a greater disruption of redox regulation and metabolic machinery in hybrid newts, which can be interpreted as the cost of hybridization that is likely linked to parental incompatibilities expressed under a higher temperature. Our study aims to improve mechanistic understanding of the resilience and distribution of hybrid species that cope with climate-driven changes.
PB  - Elsevier Ltd
T2  - Journal of Thermal Biology
T1  - Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts
VL  - 112
DO  - 10.1016/j.jtherbio.2023.103474
SP  - 103474
ER  - 

@article{
author = "Petrović, Tamara and Vučić, Tijana and Burraco, Pablo and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Radovanović, Tijana and Šajkunić, Sanja and Ivanović, Ana and Prokić, Marko",
year = "2023",
abstract = "Ectotherms are particularly sensitive to global warming due to their limited capacity to thermoregulate, which can impact their performance and fitness. From a physiological standpoint, higher temperatures often enhance biological processes that can induce the production of reactive oxygen species and result in a state of cellular oxidative stress. Temperature alters interspecific interactions, including species hybridization. Hybridization under different thermal conditions could amplify parental (genetic) incompatibilities, thus affecting a hybrid's development and distribution. Understanding the impact of global warming on the physiology of hybrids and particularly their oxidative status could help in predicting future scenarios in ecosystems and in hybrids. In the present study, we investigated the effect of water temperature on the development, growth and oxidative stress of two crested newt species and their reciprocal hybrids. Larvae of Triturus macedonicus and T. ivanbureschi, and their T. macedonicus-mothered and T. ivanbureschi-mothered hybrids were exposed for 30 days to temperatures of 19°C and 24°C. Under the higher temperature, the hybrids experienced increases in both growth and developmental rates, while parental species exhibited accelerated growth (T. macedonicus) or development (T. ivanbureschi). Warm conditions also had different effects on the oxidative status of hybrid and parental species. Parental species had enhanced antioxidant responses (catalase, glutathione peroxidase, glutathione S-transferase and SH groups), which allowed them to alleviate temperature-induced stress (revealed by the absence of oxidative damage). However, warming induced an antioxidant response in the hybrids, including oxidative damage in the form of lipid peroxidation. These findings point to a greater disruption of redox regulation and metabolic machinery in hybrid newts, which can be interpreted as the cost of hybridization that is likely linked to parental incompatibilities expressed under a higher temperature. Our study aims to improve mechanistic understanding of the resilience and distribution of hybrid species that cope with climate-driven changes.",
publisher = "Elsevier Ltd",
journal = "Journal of Thermal Biology",
title = "Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts",
volume = "112",
doi = "10.1016/j.jtherbio.2023.103474",
pages = "103474"
}

Petrović, T., Vučić, T., Burraco, P., Gavrilović, B., Despotović, S., Gavrić, J., Radovanović, T., Šajkunić, S., Ivanović, A.,& Prokić, M.. (2023). Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts. in Journal of Thermal Biology
Elsevier Ltd., 112, 103474.
https://doi.org/10.1016/j.jtherbio.2023.103474

Petrović T, Vučić T, Burraco P, Gavrilović B, Despotović S, Gavrić J, Radovanović T, Šajkunić S, Ivanović A, Prokić M. Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts. in Journal of Thermal Biology. 2023;112:103474.
doi:10.1016/j.jtherbio.2023.103474 .

Petrović, Tamara, Vučić, Tijana, Burraco, Pablo, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Radovanović, Tijana, Šajkunić, Sanja, Ivanović, Ana, Prokić, Marko, "Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts" in Journal of Thermal Biology, 112 (2023):103474,
https://doi.org/10.1016/j.jtherbio.2023.103474 . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Gašić, Uroš
AU  - Šajkunić, Sanja
AU  - Cingel, Aleksandar
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/3/407
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4789
AB  - French marigold is an aromatic plant rich in polyphenolic secondary metabolites, which pesticidal potential was examined in this study. Ultra-high-performance liquid chromatography (UHPLC) connected with OrbiTrap mass spectrometer (MS) identified 113 phenolics and revealed the most detailed phytochemistry of French marigold published so far. Depending on plant material (flowers or leaves) and solvents used for extraction (water, methanol, dichloromethane), the phenolic composition varied. Methanol extract of flowers, with 89 identified phenolics and high antiox-idant activity statistically comparable with positive control Trolox, was chosen for testing of anti-feedant potential against the 3rd and 4th instars of Colorado potato beetle (CPB). A significant reduction in final body mass of 4th larval stage fed with potato leaves coated with methanol extract of flowers in the concentration of 10 mg/mL was observed (157.67 mg vs. 182.26 mg of controls fed with non-treated leaves). This caused delayed molting since treated larvae reached the maximal mass a day after controls and this delay persisted during the entire larval development. Continuous feeding caused a 25% decline in digestive proteolytic activity of the 4th instar in comparison to controls. The results suggest that French marigold methanol extract of flowers could be proposed as a promising antifeedant for CPB management, with an impact on the reduction in the environmental footprint associated with synthetic pesticide application.
PB  - Basel: MDPI
T2  - Plants
T1  - UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle
IS  - 3
VL  - 11
DO  - 10.3390/plants11030407
SP  - 407
ER  - 

@article{
author = "Devrnja, Nina and Gašić, Uroš and Šajkunić, Sanja and Cingel, Aleksandar and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena",
year = "2022",
abstract = "French marigold is an aromatic plant rich in polyphenolic secondary metabolites, which pesticidal potential was examined in this study. Ultra-high-performance liquid chromatography (UHPLC) connected with OrbiTrap mass spectrometer (MS) identified 113 phenolics and revealed the most detailed phytochemistry of French marigold published so far. Depending on plant material (flowers or leaves) and solvents used for extraction (water, methanol, dichloromethane), the phenolic composition varied. Methanol extract of flowers, with 89 identified phenolics and high antiox-idant activity statistically comparable with positive control Trolox, was chosen for testing of anti-feedant potential against the 3rd and 4th instars of Colorado potato beetle (CPB). A significant reduction in final body mass of 4th larval stage fed with potato leaves coated with methanol extract of flowers in the concentration of 10 mg/mL was observed (157.67 mg vs. 182.26 mg of controls fed with non-treated leaves). This caused delayed molting since treated larvae reached the maximal mass a day after controls and this delay persisted during the entire larval development. Continuous feeding caused a 25% decline in digestive proteolytic activity of the 4th instar in comparison to controls. The results suggest that French marigold methanol extract of flowers could be proposed as a promising antifeedant for CPB management, with an impact on the reduction in the environmental footprint associated with synthetic pesticide application.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle",
number = "3",
volume = "11",
doi = "10.3390/plants11030407",
pages = "407"
}

Devrnja, N., Gašić, U., Šajkunić, S., Cingel, A., Stupar, S., Tubić, L.,& Savić, J.. (2022). UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle. in Plants
Basel: MDPI., 11(3), 407.
https://doi.org/10.3390/plants11030407

Devrnja N, Gašić U, Šajkunić S, Cingel A, Stupar S, Tubić L, Savić J. UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle. in Plants. 2022;11(3):407.
doi:10.3390/plants11030407 .

Devrnja, Nina, Gašić, Uroš, Šajkunić, Sanja, Cingel, Aleksandar, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, "UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle" in Plants, 11, no. 3 (2022):407,
https://doi.org/10.3390/plants11030407 . .

TY  - CONF
AU  - Šajkunić, Sanja
AU  - Dragićević, Milan
AU  - Milutinović, Milica
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
AU  - Devrnja, Nina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5050
AB  - Different stressors from the environment can prime defense and enable enhanced responses
to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the
level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown
promising prospects as novel priming inducers. Still, little is known about their potential to induce
epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula
L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to
EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal.
Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM)
DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR,
and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and
treated samples were analyzed. The average methylation level of the whole genome was 72.29%
and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly
occurred in the CG context compared to CHH and CHG. In general, hypomethylated
sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially
methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein
processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes
associated with these pathways, as well as different defense responses in potato (GO:0006952),
will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress
conditions.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - French marigold essential oil affected DNA methylation in potato
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5050
ER  - 

@conference{
author = "Šajkunić, Sanja and Dragićević, Milan and Milutinović, Milica and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena and Devrnja, Nina",
year = "2022",
abstract = "Different stressors from the environment can prime defense and enable enhanced responses
to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the
level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown
promising prospects as novel priming inducers. Still, little is known about their potential to induce
epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula
L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to
EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal.
Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM)
DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR,
and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and
treated samples were analyzed. The average methylation level of the whole genome was 72.29%
and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly
occurred in the CG context compared to CHH and CHG. In general, hypomethylated
sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially
methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein
processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes
associated with these pathways, as well as different defense responses in potato (GO:0006952),
will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress
conditions.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "French marigold essential oil affected DNA methylation in potato",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5050"
}

Šajkunić, S., Dragićević, M., Milutinović, M., Stupar, S., Tubić, L., Savić, J.,& Devrnja, N.. (2022). French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 71.
https://hdl.handle.net/21.15107/rcub_ibiss_5050

Šajkunić S, Dragićević M, Milutinović M, Stupar S, Tubić L, Savić J, Devrnja N. French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:71.
https://hdl.handle.net/21.15107/rcub_ibiss_5050 .

Šajkunić, Sanja, Dragićević, Milan, Milutinović, Milica, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, Devrnja, Nina, "French marigold essential oil affected DNA methylation in potato" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):71,
https://hdl.handle.net/21.15107/rcub_ibiss_5050 .

TY  - CONF
AU  - Devrnja, Nina
AU  - Šajkunić, Sanja
AU  - Anđelković, Boban
AU  - Tešević, Vele
AU  - Milutinović, Milica
AU  - Skorić, Marijana
AU  - Savić, Jelena
PY  - 2022
UR  - https://www.plantslo.org/wp/wp-content/uploads/2022/09/PlantsInChangingEnvironment22AbstractBook.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5054
AB  - Essential oils (EOs) obtained from plants can frequently exhibit toxicity and repellency/deterrence against insect pests. Fennel (Foeniculum vulgare Mill.) and basil (Ocimum basilicum L.) EOs are used in pest management applications, but the most-discussed constraint regards restraining the usage of these natural insecticides in common agricultural practices is related to the volatility and limited persistence of EOs under field conditions. Due to high volatility, the efficacy of these substances falls short after EO application and requires frequent reapplication. There is a great interest in developing formulations that improve the handling of EOs, prevent the photodegradation of their active ingredients, and control the release of volatiles through micro/nanospheres or capsules in solid or liquid form. The polymer-based EO nanoformulations provide the stability and optimal effectiveness of EOs with an anti-insecticidal effect. One of the main advantages of solid formulations is that they can have a longer shelf life and be applied in solid form or even mixed with water and sprayed. We investigated the formation and characterization of inclusion complexes (ICs) of beta-cyclodextrin (β-CD) with two selected EOs, using the co-precipitation method. The main constituents of EOs determined by GC-MS analyses were trans-anethole (37.77%) and α-pinene (23.51%) in fennel EO, and linalool (32.6%) and methyl chavicol (23.2%) in basil EO. The interaction between β-CD and EO molecules was assessed through Fourier-transform infrared spectroscopy (FT-IR) and it was used to characterize the ICs by evaluating the changes recorded in the ICs compared to the free β-CD and individual EO. FT-IR spectra of inclusion complexes displayed peak shifts and variations of intensity when it compared to FT-IR spectra of EOs and free β-CD. β-cyclodextrin resulted as an effective host in encapsulating selected EOs. The potato plants sprayed with ICs were examined by scanning electron microscopy (SEM) and showed ICs with lamellate or rhomboid morphology. Bioassay with Colorado potato beetle fed with potato plants covered with ICs showed that complexation with β-CD reduced insects’ growth (body mass of IV instar larvae where 119.84±1.48 mg and 120.79±7.42 mg for IC with fennel and basil EO respectively in comparison to body mass of control larvae 138.97±2.01mg) and affected digestive proteolytic activity.
PB  - Ljubljana: Slovenian Society of Plant Biology
C3  - Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
T1  - Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth
SP  - 7
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5054
ER  - 

@conference{
author = "Devrnja, Nina and Šajkunić, Sanja and Anđelković, Boban and Tešević, Vele and Milutinović, Milica and Skorić, Marijana and Savić, Jelena",
year = "2022",
abstract = "Essential oils (EOs) obtained from plants can frequently exhibit toxicity and repellency/deterrence against insect pests. Fennel (Foeniculum vulgare Mill.) and basil (Ocimum basilicum L.) EOs are used in pest management applications, but the most-discussed constraint regards restraining the usage of these natural insecticides in common agricultural practices is related to the volatility and limited persistence of EOs under field conditions. Due to high volatility, the efficacy of these substances falls short after EO application and requires frequent reapplication. There is a great interest in developing formulations that improve the handling of EOs, prevent the photodegradation of their active ingredients, and control the release of volatiles through micro/nanospheres or capsules in solid or liquid form. The polymer-based EO nanoformulations provide the stability and optimal effectiveness of EOs with an anti-insecticidal effect. One of the main advantages of solid formulations is that they can have a longer shelf life and be applied in solid form or even mixed with water and sprayed. We investigated the formation and characterization of inclusion complexes (ICs) of beta-cyclodextrin (β-CD) with two selected EOs, using the co-precipitation method. The main constituents of EOs determined by GC-MS analyses were trans-anethole (37.77%) and α-pinene (23.51%) in fennel EO, and linalool (32.6%) and methyl chavicol (23.2%) in basil EO. The interaction between β-CD and EO molecules was assessed through Fourier-transform infrared spectroscopy (FT-IR) and it was used to characterize the ICs by evaluating the changes recorded in the ICs compared to the free β-CD and individual EO. FT-IR spectra of inclusion complexes displayed peak shifts and variations of intensity when it compared to FT-IR spectra of EOs and free β-CD. β-cyclodextrin resulted as an effective host in encapsulating selected EOs. The potato plants sprayed with ICs were examined by scanning electron microscopy (SEM) and showed ICs with lamellate or rhomboid morphology. Bioassay with Colorado potato beetle fed with potato plants covered with ICs showed that complexation with β-CD reduced insects’ growth (body mass of IV instar larvae where 119.84±1.48 mg and 120.79±7.42 mg for IC with fennel and basil EO respectively in comparison to body mass of control larvae 138.97±2.01mg) and affected digestive proteolytic activity.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.",
title = "Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth",
pages = "7",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5054"
}

Devrnja, N., Šajkunić, S., Anđelković, B., Tešević, V., Milutinović, M., Skorić, M.,& Savić, J.. (2022). Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth. in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
Ljubljana: Slovenian Society of Plant Biology., 7.
https://hdl.handle.net/21.15107/rcub_ibiss_5054

Devrnja N, Šajkunić S, Anđelković B, Tešević V, Milutinović M, Skorić M, Savić J. Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth. in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.. 2022;:7.
https://hdl.handle.net/21.15107/rcub_ibiss_5054 .

Devrnja, Nina, Šajkunić, Sanja, Anđelković, Boban, Tešević, Vele, Milutinović, Milica, Skorić, Marijana, Savić, Jelena, "Characterization of β-cyclodextrin - fennel and basil essential oil inclusion complexes and effect on Colorado potato beetle growth" in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. (2022):7,
https://hdl.handle.net/21.15107/rcub_ibiss_5054 .

TY  - CONF
AU  - Šajkunić, Sanja
AU  - Dragićević, Milan
AU  - Milutinović, Milica
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
AU  - Devrnja, Nina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5051
AB  - Different stressors from the environment can prime defense and enable enhanced responses to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown promising prospects as novel priming inducers. Still, little is known about their potential to induce epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal. Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM) DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR, and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and treated samples were analyzed. The average methylation level of the whole genome was 72.29% and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly occurred in the CG context compared to CHH and CHG. In general, hypomethylated sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes associated with these pathways, as well as different defense responses in potato (GO:0006952), will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress conditions.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.
T1  - French marigold essential oil affected DNA methylation in potato
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5051
ER  - 

@conference{
author = "Šajkunić, Sanja and Dragićević, Milan and Milutinović, Milica and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena and Devrnja, Nina",
year = "2022",
abstract = "Different stressors from the environment can prime defense and enable enhanced responses to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown promising prospects as novel priming inducers. Still, little is known about their potential to induce epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal. Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM) DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR, and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and treated samples were analyzed. The average methylation level of the whole genome was 72.29% and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly occurred in the CG context compared to CHH and CHG. In general, hypomethylated sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes associated with these pathways, as well as different defense responses in potato (GO:0006952), will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress conditions.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.",
title = "French marigold essential oil affected DNA methylation in potato",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5051"
}

Šajkunić, S., Dragićević, M., Milutinović, M., Stupar, S., Tubić, L., Savić, J.,& Devrnja, N.. (2022). French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5051

Šajkunić S, Dragićević M, Milutinović M, Stupar S, Tubić L, Savić J, Devrnja N. French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5051 .

Šajkunić, Sanja, Dragićević, Milan, Milutinović, Milica, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, Devrnja, Nina, "French marigold essential oil affected DNA methylation in potato" in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5051 .

TY  - CONF
AU  - Savić, Jelena
AU  - Stupar, Sofija
AU  - Devrnja, Nina
AU  - Šajkunić, Sanja
AU  - Tubić, Ljiljana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5053
AB  - Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
T1  - Role of glutathione in potato response to French marigold essential oil
SP  - P6
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5053
ER  - 

@conference{
author = "Savić, Jelena and Stupar, Sofija and Devrnja, Nina and Šajkunić, Sanja and Tubić, Ljiljana",
year = "2022",
abstract = "Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.",
title = "Role of glutathione in potato response to French marigold essential oil",
pages = "P6",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5053"
}

Savić, J., Stupar, S., Devrnja, N., Šajkunić, S.,& Tubić, L.. (2022). Role of glutathione in potato response to French marigold essential oil. in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., P6.
https://hdl.handle.net/21.15107/rcub_ibiss_5053

Savić J, Stupar S, Devrnja N, Šajkunić S, Tubić L. Role of glutathione in potato response to French marigold essential oil. in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.. 2022;:P6.
https://hdl.handle.net/21.15107/rcub_ibiss_5053 .

Savić, Jelena, Stupar, Sofija, Devrnja, Nina, Šajkunić, Sanja, Tubić, Ljiljana, "Role of glutathione in potato response to French marigold essential oil" in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. (2022):P6,
https://hdl.handle.net/21.15107/rcub_ibiss_5053 .

TY  - CONF
AU  - Savić, Jelena
AU  - Stupar, Sofija
AU  - Devrnja, Nina
AU  - Šajkunić, Sanja
AU  - Tubić, Ljiljana
PY  - 2022
UR  - https://www.plantslo.org/wp/wp-content/uploads/2022/09/PlantsInChangingEnvironment22AbstractBook.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5052
AB  - Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.
PB  - Ljubljana: Slovenian Society of Plant Biology
C3  - Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
T1  - Role of glutathione in potato response to French marigold essential oil
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5052
ER  - 

@conference{
author = "Savić, Jelena and Stupar, Sofija and Devrnja, Nina and Šajkunić, Sanja and Tubić, Ljiljana",
year = "2022",
abstract = "Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.",
title = "Role of glutathione in potato response to French marigold essential oil",
pages = "17",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5052"
}

Savić, J., Stupar, S., Devrnja, N., Šajkunić, S.,& Tubić, L.. (2022). Role of glutathione in potato response to French marigold essential oil. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
Ljubljana: Slovenian Society of Plant Biology., 17.
https://hdl.handle.net/21.15107/rcub_ibiss_5052

Savić J, Stupar S, Devrnja N, Šajkunić S, Tubić L. Role of glutathione in potato response to French marigold essential oil. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.. 2022;:17.
https://hdl.handle.net/21.15107/rcub_ibiss_5052 .

Savić, Jelena, Stupar, Sofija, Devrnja, Nina, Šajkunić, Sanja, Tubić, Ljiljana, "Role of glutathione in potato response to French marigold essential oil" in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. (2022):17,
https://hdl.handle.net/21.15107/rcub_ibiss_5052 .

TY  - CONF
AU  - Devrnja, Nina
AU  - Ćosić, Tatjana
AU  - Stupar, Sofija
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Šajkunić, Sanja
AU  - Ćirić, Ana
AU  - Savić, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5594
AB  - Methanol and aqueous extracts of French marigold flowers and leaves, with previously determined chemical profiles, were subjected to in vitro antioxidant and antimicrobial assays. The methanol extract of flowers had the highest total phenolic content (TPC) according to the Folin–Ciocalteu method, and UHPLC–LTQ/orbitrap/MS analysis identified the flavonoid aglycones as the dominant class of phenolics in the extract. Likewise, the methanol extract of flowers showed the highest antioxidant activity measured by DPPH and ABTS assays. Generally, the higher TPC correlated with lower IC50 value and stronger antioxidant potential in all extracts. Microdilution test was used for determination of the minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations of French marigold extracts on following microfungi - Aspergillus versicolor, A. niger, Penicillium ochrochloron, P. verrucosum var. cyclopium and Alternaria alternata. The antimicrobial test revealed that P. ochrochloron was the most sensitive while A. niger was the most resistant microfungus to all analyzed extracts. Correspondingly, the methanol extract of flowers was shown to be the most potent one, regarding the antimicrobial activity. Additionally, antimicrobial potential of methanol extract of flowers was tested for in vivo potato tuber protection against A. alternata causing the early blight disease. Based on obtained antioxidant and antimicrobial potential, French marigold methanol extracts could provide an alternative strategy for the controlling expansion of potato pathogens.
PB  - European Federation of Biotechnology
C3  - Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
T1  - Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts
SP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5594
ER  - 

@conference{
author = "Devrnja, Nina and Ćosić, Tatjana and Stupar, Sofija and Gašić, Uroš and Tešić, Živoslav and Šajkunić, Sanja and Ćirić, Ana and Savić, Jelena",
year = "2019",
abstract = "Methanol and aqueous extracts of French marigold flowers and leaves, with previously determined chemical profiles, were subjected to in vitro antioxidant and antimicrobial assays. The methanol extract of flowers had the highest total phenolic content (TPC) according to the Folin–Ciocalteu method, and UHPLC–LTQ/orbitrap/MS analysis identified the flavonoid aglycones as the dominant class of phenolics in the extract. Likewise, the methanol extract of flowers showed the highest antioxidant activity measured by DPPH and ABTS assays. Generally, the higher TPC correlated with lower IC50 value and stronger antioxidant potential in all extracts. Microdilution test was used for determination of the minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations of French marigold extracts on following microfungi - Aspergillus versicolor, A. niger, Penicillium ochrochloron, P. verrucosum var. cyclopium and Alternaria alternata. The antimicrobial test revealed that P. ochrochloron was the most sensitive while A. niger was the most resistant microfungus to all analyzed extracts. Correspondingly, the methanol extract of flowers was shown to be the most potent one, regarding the antimicrobial activity. Additionally, antimicrobial potential of methanol extract of flowers was tested for in vivo potato tuber protection against A. alternata causing the early blight disease. Based on obtained antioxidant and antimicrobial potential, French marigold methanol extracts could provide an alternative strategy for the controlling expansion of potato pathogens.",
publisher = "European Federation of Biotechnology",
journal = "Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic",
title = "Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts",
pages = "130",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5594"
}

Devrnja, N., Ćosić, T., Stupar, S., Gašić, U., Tešić, Ž., Šajkunić, S., Ćirić, A.,& Savić, J.. (2019). Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
European Federation of Biotechnology., 130.
https://hdl.handle.net/21.15107/rcub_ibiss_5594

Devrnja N, Ćosić T, Stupar S, Gašić U, Tešić Ž, Šajkunić S, Ćirić A, Savić J. Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic. 2019;:130.
https://hdl.handle.net/21.15107/rcub_ibiss_5594 .

Devrnja, Nina, Ćosić, Tatjana, Stupar, Sofija, Gašić, Uroš, Tešić, Živoslav, Šajkunić, Sanja, Ćirić, Ana, Savić, Jelena, "Estimation of in vitro antioxidant and antimicrobial potential of French marigold (Tagetes patula L.) extracts" in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic (2019):130,
https://hdl.handle.net/21.15107/rcub_ibiss_5594 .