TY  - JOUR
AU  - Milanović, Desanka
AU  - Petrovic, Snjezana
AU  - Brkić, Marjana
AU  - Avramović, Vladimir
AU  - Perović, Milka
AU  - Ivković, Sanja
AU  - Glibetić, Marija
AU  - Kanazir, Selma
PY  - 2018
UR  - http://www.mdpi.com/2072-6643/10/9/1250
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3136
AB  - Long-term fish oil (FO) supplementation is able to improve Alzheimer's disease (AD) pathology. We aimed to determine the impact of short-term fish oil (FO) intake on phospholipids composition and plaque pathology in 5xFAD mice, a widely used animal model of AD. A 3-week-long FO supplementation administered at 3 months of age decreased the number of dense core plaques in the 5xFAD cortex and changed phospholipids in the livers and brains of wild-type (Wt) and 5xFAD mice. Livers of both genotypes responded by increase of n-3 and reciprocal decrease of n-6 fatty acids. In Wt brains, FO supplementation induced elevation of n-3 fatty acids and subsequent enhancement of n-6/n-3 ratio. However, in 5xFAD brains the improved n-6/n-3 ratio was mainly due to FO-induced decrease in arachidonic and adrenic n-6 fatty acids. Also, brain and liver abundance of n-3 fatty acids were strongly correlated in Wts, oppositely to 5xFADs where significant brain-liver correlation exists only for n-6 fatty acids. Expression of omega-3 transporter Mfs2a remained unchanged after FO supplementation. We have demonstrated that even a short-term FO intake improves the phospholipid composition and has a significant effect on plaque burden in 5xFAD brains when applied in early stages of AD pathology.
T2  - Nutrients
T1  - Short-Term Fish Oil Treatment Changes the Composition of Phospholipids While Not Affecting the Expression of Mfsd2a Omega-3 Transporter in the Brain and Liver of the 5xFAD Mouse Model of Alzheimer's Disease.
IS  - 9
VL  - 10
DO  - 10.3390/nu10091250
SP  - 1250
ER  - 

@article{
author = "Milanović, Desanka and Petrovic, Snjezana and Brkić, Marjana and Avramović, Vladimir and Perović, Milka and Ivković, Sanja and Glibetić, Marija and Kanazir, Selma",
year = "2018",
abstract = "Long-term fish oil (FO) supplementation is able to improve Alzheimer's disease (AD) pathology. We aimed to determine the impact of short-term fish oil (FO) intake on phospholipids composition and plaque pathology in 5xFAD mice, a widely used animal model of AD. A 3-week-long FO supplementation administered at 3 months of age decreased the number of dense core plaques in the 5xFAD cortex and changed phospholipids in the livers and brains of wild-type (Wt) and 5xFAD mice. Livers of both genotypes responded by increase of n-3 and reciprocal decrease of n-6 fatty acids. In Wt brains, FO supplementation induced elevation of n-3 fatty acids and subsequent enhancement of n-6/n-3 ratio. However, in 5xFAD brains the improved n-6/n-3 ratio was mainly due to FO-induced decrease in arachidonic and adrenic n-6 fatty acids. Also, brain and liver abundance of n-3 fatty acids were strongly correlated in Wts, oppositely to 5xFADs where significant brain-liver correlation exists only for n-6 fatty acids. Expression of omega-3 transporter Mfs2a remained unchanged after FO supplementation. We have demonstrated that even a short-term FO intake improves the phospholipid composition and has a significant effect on plaque burden in 5xFAD brains when applied in early stages of AD pathology.",
journal = "Nutrients",
title = "Short-Term Fish Oil Treatment Changes the Composition of Phospholipids While Not Affecting the Expression of Mfsd2a Omega-3 Transporter in the Brain and Liver of the 5xFAD Mouse Model of Alzheimer's Disease.",
number = "9",
volume = "10",
doi = "10.3390/nu10091250",
pages = "1250"
}

Milanović, D., Petrovic, S., Brkić, M., Avramović, V., Perović, M., Ivković, S., Glibetić, M.,& Kanazir, S.. (2018). Short-Term Fish Oil Treatment Changes the Composition of Phospholipids While Not Affecting the Expression of Mfsd2a Omega-3 Transporter in the Brain and Liver of the 5xFAD Mouse Model of Alzheimer's Disease.. in Nutrients, 10(9), 1250.
https://doi.org/10.3390/nu10091250

Milanović D, Petrovic S, Brkić M, Avramović V, Perović M, Ivković S, Glibetić M, Kanazir S. Short-Term Fish Oil Treatment Changes the Composition of Phospholipids While Not Affecting the Expression of Mfsd2a Omega-3 Transporter in the Brain and Liver of the 5xFAD Mouse Model of Alzheimer's Disease.. in Nutrients. 2018;10(9):1250.
doi:10.3390/nu10091250 .

Milanović, Desanka, Petrovic, Snjezana, Brkić, Marjana, Avramović, Vladimir, Perović, Milka, Ivković, Sanja, Glibetić, Marija, Kanazir, Selma, "Short-Term Fish Oil Treatment Changes the Composition of Phospholipids While Not Affecting the Expression of Mfsd2a Omega-3 Transporter in the Brain and Liver of the 5xFAD Mouse Model of Alzheimer's Disease." in Nutrients, 10, no. 9 (2018):1250,
https://doi.org/10.3390/nu10091250 . .

TY  - JOUR
AU  - Petrovic, Snjezana
AU  - Milosevic, Maja
AU  - Ristic-Medic, Danijela
AU  - Velickovic, Natasa
AU  - Drakulic, Dunja
AU  - Grkovic, Ivana
AU  - Horvat, Anica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2077
AB  - Our earlier studies found that in vitro estradiol modulates
   mitochondrial Ca2+ transport in discrete brain regions. The present
   study examined the role of estradiol receptors (ERs) in
   estradiol-induced inhibition of Ca2+ efflux from synaptosomal
   mitochondria isolated from rat caudate nuclei and brain stems.
   Radioactively labeled CaCl2 (0.6-0.75 mu Ci (CaCl2)-Ca-45) was used for
   Ca2+ transport monitoring. The results revealed that in the presence of
   ER antagonist 7 alpha, 17 beta-{[}9{[}(4,4,5,5,5-pentafluoropentyl)
   sulfinyl] nonyl] estra-1,3,5( 10)-triene-3,17-diol (ICI 182,780) (1 mu
   mol/L), the inhibitory effect of estradiol on mitochondrial Ca2+ efflux
   was more than 60\% decreased, suggesting the involvement of ER in this
   mode of estradiol neuromodulatory action. When particular contributions
   of ER alpha and ER beta were tested, it was found that ER beta agonist
   2,3-bis(4-hydroxy phenyl)-propionitrile (10 nmol/L) inhibited Ca2+
   efflux more than 20\%, while the inhibition with ER alpha agonist 4,4',
   4''-(4-propyl-{[}1H]-pyrazole-1,3,5-triyl) trisphenol (10 nmol/L) was
   about 10\%, both compared to the control. Both agonists demonstrated
   attenuation of Ca2+ efflux decrease in the presence of mitochondrial
   Na+/Ca2+ exchanger antagonist
   7-chloro-5-(2-chlorophenyl)-1,5-dihyhdro-4,1-benzothiazepin-2(3H)-one
   (10 mu mol/L), showing interference with the inhibitory action of that
   agent. Our results strongly indicate ERs as the mediators of
   estradiol-induced mitochondrial Ca2+ efflux inhibition in rat caudate
   nucleus and brain stem synaptosomes.
T2  - Turkish Journal of Biology
T1  - Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem
IS  - 2
VL  - 39
DO  - 10.3906/biy-1408-62
SP  - 328
EP  - 334
ER  - 

@article{
author = "Petrovic, Snjezana and Milosevic, Maja and Ristic-Medic, Danijela and Velickovic, Natasa and Drakulic, Dunja and Grkovic, Ivana and Horvat, Anica",
year = "2015",
abstract = "Our earlier studies found that in vitro estradiol modulates
   mitochondrial Ca2+ transport in discrete brain regions. The present
   study examined the role of estradiol receptors (ERs) in
   estradiol-induced inhibition of Ca2+ efflux from synaptosomal
   mitochondria isolated from rat caudate nuclei and brain stems.
   Radioactively labeled CaCl2 (0.6-0.75 mu Ci (CaCl2)-Ca-45) was used for
   Ca2+ transport monitoring. The results revealed that in the presence of
   ER antagonist 7 alpha, 17 beta-{[}9{[}(4,4,5,5,5-pentafluoropentyl)
   sulfinyl] nonyl] estra-1,3,5( 10)-triene-3,17-diol (ICI 182,780) (1 mu
   mol/L), the inhibitory effect of estradiol on mitochondrial Ca2+ efflux
   was more than 60\% decreased, suggesting the involvement of ER in this
   mode of estradiol neuromodulatory action. When particular contributions
   of ER alpha and ER beta were tested, it was found that ER beta agonist
   2,3-bis(4-hydroxy phenyl)-propionitrile (10 nmol/L) inhibited Ca2+
   efflux more than 20\%, while the inhibition with ER alpha agonist 4,4',
   4''-(4-propyl-{[}1H]-pyrazole-1,3,5-triyl) trisphenol (10 nmol/L) was
   about 10\%, both compared to the control. Both agonists demonstrated
   attenuation of Ca2+ efflux decrease in the presence of mitochondrial
   Na+/Ca2+ exchanger antagonist
   7-chloro-5-(2-chlorophenyl)-1,5-dihyhdro-4,1-benzothiazepin-2(3H)-one
   (10 mu mol/L), showing interference with the inhibitory action of that
   agent. Our results strongly indicate ERs as the mediators of
   estradiol-induced mitochondrial Ca2+ efflux inhibition in rat caudate
   nucleus and brain stem synaptosomes.",
journal = "Turkish Journal of Biology",
title = "Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem",
number = "2",
volume = "39",
doi = "10.3906/biy-1408-62",
pages = "328-334"
}

Petrovic, S., Milosevic, M., Ristic-Medic, D., Velickovic, N., Drakulic, D., Grkovic, I.,& Horvat, A.. (2015). Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem. in Turkish Journal of Biology, 39(2), 328-334.
https://doi.org/10.3906/biy-1408-62

Petrovic S, Milosevic M, Ristic-Medic D, Velickovic N, Drakulic D, Grkovic I, Horvat A. Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem. in Turkish Journal of Biology. 2015;39(2):328-334.
doi:10.3906/biy-1408-62 .

Petrovic, Snjezana, Milosevic, Maja, Ristic-Medic, Danijela, Velickovic, Natasa, Drakulic, Dunja, Grkovic, Ivana, Horvat, Anica, "Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem" in Turkish Journal of Biology, 39, no. 2 (2015):328-334,
https://doi.org/10.3906/biy-1408-62 . .