TY  - JOUR
AU  - Petrovic, Snjezana
AU  - Milosevic, Maja
AU  - Ristic-Medic, Danijela
AU  - Velickovic, Natasa
AU  - Drakulic, Dunja
AU  - Grkovic, Ivana
AU  - Horvat, Anica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2077
AB  - Our earlier studies found that in vitro estradiol modulates
   mitochondrial Ca2+ transport in discrete brain regions. The present
   study examined the role of estradiol receptors (ERs) in
   estradiol-induced inhibition of Ca2+ efflux from synaptosomal
   mitochondria isolated from rat caudate nuclei and brain stems.
   Radioactively labeled CaCl2 (0.6-0.75 mu Ci (CaCl2)-Ca-45) was used for
   Ca2+ transport monitoring. The results revealed that in the presence of
   ER antagonist 7 alpha, 17 beta-{[}9{[}(4,4,5,5,5-pentafluoropentyl)
   sulfinyl] nonyl] estra-1,3,5( 10)-triene-3,17-diol (ICI 182,780) (1 mu
   mol/L), the inhibitory effect of estradiol on mitochondrial Ca2+ efflux
   was more than 60\% decreased, suggesting the involvement of ER in this
   mode of estradiol neuromodulatory action. When particular contributions
   of ER alpha and ER beta were tested, it was found that ER beta agonist
   2,3-bis(4-hydroxy phenyl)-propionitrile (10 nmol/L) inhibited Ca2+
   efflux more than 20\%, while the inhibition with ER alpha agonist 4,4',
   4''-(4-propyl-{[}1H]-pyrazole-1,3,5-triyl) trisphenol (10 nmol/L) was
   about 10\%, both compared to the control. Both agonists demonstrated
   attenuation of Ca2+ efflux decrease in the presence of mitochondrial
   Na+/Ca2+ exchanger antagonist
   7-chloro-5-(2-chlorophenyl)-1,5-dihyhdro-4,1-benzothiazepin-2(3H)-one
   (10 mu mol/L), showing interference with the inhibitory action of that
   agent. Our results strongly indicate ERs as the mediators of
   estradiol-induced mitochondrial Ca2+ efflux inhibition in rat caudate
   nucleus and brain stem synaptosomes.
T2  - Turkish Journal of Biology
T1  - Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem
IS  - 2
VL  - 39
DO  - 10.3906/biy-1408-62
SP  - 328
EP  - 334
ER  - 

@article{
author = "Petrovic, Snjezana and Milosevic, Maja and Ristic-Medic, Danijela and Velickovic, Natasa and Drakulic, Dunja and Grkovic, Ivana and Horvat, Anica",
year = "2015",
abstract = "Our earlier studies found that in vitro estradiol modulates
   mitochondrial Ca2+ transport in discrete brain regions. The present
   study examined the role of estradiol receptors (ERs) in
   estradiol-induced inhibition of Ca2+ efflux from synaptosomal
   mitochondria isolated from rat caudate nuclei and brain stems.
   Radioactively labeled CaCl2 (0.6-0.75 mu Ci (CaCl2)-Ca-45) was used for
   Ca2+ transport monitoring. The results revealed that in the presence of
   ER antagonist 7 alpha, 17 beta-{[}9{[}(4,4,5,5,5-pentafluoropentyl)
   sulfinyl] nonyl] estra-1,3,5( 10)-triene-3,17-diol (ICI 182,780) (1 mu
   mol/L), the inhibitory effect of estradiol on mitochondrial Ca2+ efflux
   was more than 60\% decreased, suggesting the involvement of ER in this
   mode of estradiol neuromodulatory action. When particular contributions
   of ER alpha and ER beta were tested, it was found that ER beta agonist
   2,3-bis(4-hydroxy phenyl)-propionitrile (10 nmol/L) inhibited Ca2+
   efflux more than 20\%, while the inhibition with ER alpha agonist 4,4',
   4''-(4-propyl-{[}1H]-pyrazole-1,3,5-triyl) trisphenol (10 nmol/L) was
   about 10\%, both compared to the control. Both agonists demonstrated
   attenuation of Ca2+ efflux decrease in the presence of mitochondrial
   Na+/Ca2+ exchanger antagonist
   7-chloro-5-(2-chlorophenyl)-1,5-dihyhdro-4,1-benzothiazepin-2(3H)-one
   (10 mu mol/L), showing interference with the inhibitory action of that
   agent. Our results strongly indicate ERs as the mediators of
   estradiol-induced mitochondrial Ca2+ efflux inhibition in rat caudate
   nucleus and brain stem synaptosomes.",
journal = "Turkish Journal of Biology",
title = "Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem",
number = "2",
volume = "39",
doi = "10.3906/biy-1408-62",
pages = "328-334"
}

Petrovic, S., Milosevic, M., Ristic-Medic, D., Velickovic, N., Drakulic, D., Grkovic, I.,& Horvat, A.. (2015). Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem. in Turkish Journal of Biology, 39(2), 328-334.
https://doi.org/10.3906/biy-1408-62

Petrovic S, Milosevic M, Ristic-Medic D, Velickovic N, Drakulic D, Grkovic I, Horvat A. Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem. in Turkish Journal of Biology. 2015;39(2):328-334.
doi:10.3906/biy-1408-62 .

Petrovic, Snjezana, Milosevic, Maja, Ristic-Medic, Danijela, Velickovic, Natasa, Drakulic, Dunja, Grkovic, Ivana, Horvat, Anica, "Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem" in Turkish Journal of Biology, 39, no. 2 (2015):328-334,
https://doi.org/10.3906/biy-1408-62 . .