TY  - JOUR
AU  - Perić, Mirjana
AU  - Čairović, Aleksandra
AU  - Špadijer Gostović, Aleksandra
AU  - Stanimirović, Dragan
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Pavlović, Vladimir B.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6005
AB  - The purpose of this study was to evaluate the surface texture and biofilm adhe sion of veneered or CAD/CAM milled zirconia (partially stabilized with yttrium) after professional
oral hygiene procedures. The samples (4 × 4 mm, thickness 2 mm; n = 72) were separated from
zirconia blanks (3Y-TZP-LA). One group was veenered with ceramics, and the other group of samples
was CAD/CAM milled. Each group had two subgroups: polished and glazed. The samples were
subjected to simulated strokes of professional brushing using abrasive paste and ultrasonic scaling.
The parameters of surface micromorphology and receptivity to biofilm were calculated before and
after simulating the given methods of the professional maintenance of oral hygiene. Scanning electron
microscopy (SEM) was used to evaluate zirconia surface properties. Microbial (bacterial/fungal)
species (Staphylococcus aureus, Streptococcus sanguinis and Candida albicans) were used and cultured
on respective sterilized zirconia surfaces. Colony-forming unit (CFU) counts were used to quantify
the amount of biofilm formation on zirconia samples surfaces. Results: The SEM analysis showed
the greatest change in surface microtopography after the use of ultrasonic scaling on glazed zirconia
samples. Less formation of colonies on the surfaces of CAD/CAM milled zirconia restorations was
observed. Conclusion: Routine methods of oral hygiene professional maintenance can damage the
surfaces of glazed zirconia restorations.
PB  - Basel: MDPI
T2  - Applied Sciences
T1  - Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study
IS  - 14
VL  - 13
DO  - 10.3390/app13148389
SP  - 8389
ER  - 

@article{
author = "Perić, Mirjana and Čairović, Aleksandra and Špadijer Gostović, Aleksandra and Stanimirović, Dragan and Kostić, Marina and Ivanov, Marija and Ćirić, Ana and Glamočlija, Jasmina and Pavlović, Vladimir B.",
year = "2023",
abstract = "The purpose of this study was to evaluate the surface texture and biofilm adhe sion of veneered or CAD/CAM milled zirconia (partially stabilized with yttrium) after professional
oral hygiene procedures. The samples (4 × 4 mm, thickness 2 mm; n = 72) were separated from
zirconia blanks (3Y-TZP-LA). One group was veenered with ceramics, and the other group of samples
was CAD/CAM milled. Each group had two subgroups: polished and glazed. The samples were
subjected to simulated strokes of professional brushing using abrasive paste and ultrasonic scaling.
The parameters of surface micromorphology and receptivity to biofilm were calculated before and
after simulating the given methods of the professional maintenance of oral hygiene. Scanning electron
microscopy (SEM) was used to evaluate zirconia surface properties. Microbial (bacterial/fungal)
species (Staphylococcus aureus, Streptococcus sanguinis and Candida albicans) were used and cultured
on respective sterilized zirconia surfaces. Colony-forming unit (CFU) counts were used to quantify
the amount of biofilm formation on zirconia samples surfaces. Results: The SEM analysis showed
the greatest change in surface microtopography after the use of ultrasonic scaling on glazed zirconia
samples. Less formation of colonies on the surfaces of CAD/CAM milled zirconia restorations was
observed. Conclusion: Routine methods of oral hygiene professional maintenance can damage the
surfaces of glazed zirconia restorations.",
publisher = "Basel: MDPI",
journal = "Applied Sciences",
title = "Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study",
number = "14",
volume = "13",
doi = "10.3390/app13148389",
pages = "8389"
}

Perić, M., Čairović, A., Špadijer Gostović, A., Stanimirović, D., Kostić, M., Ivanov, M., Ćirić, A., Glamočlija, J.,& Pavlović, V. B.. (2023). Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study. in Applied Sciences
Basel: MDPI., 13(14), 8389.
https://doi.org/10.3390/app13148389

Perić M, Čairović A, Špadijer Gostović A, Stanimirović D, Kostić M, Ivanov M, Ćirić A, Glamočlija J, Pavlović VB. Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study. in Applied Sciences. 2023;13(14):8389.
doi:10.3390/app13148389 .

Perić, Mirjana, Čairović, Aleksandra, Špadijer Gostović, Aleksandra, Stanimirović, Dragan, Kostić, Marina, Ivanov, Marija, Ćirić, Ana, Glamočlija, Jasmina, Pavlović, Vladimir B., "Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study" in Applied Sciences, 13, no. 14 (2023):8389,
https://doi.org/10.3390/app13148389 . .

TY  - JOUR
AU  - Čairović, Aleksandra
AU  - Stanimirović, Dragan
AU  - Krajnović, Tamara
AU  - Dojčinović, Biljana
AU  - Maksimović, Vesna
AU  - Cvijović-Alagić, Ivana
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641900017C
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4072
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3394
AB  - The biological quality and chemical composition of alloys used in dental practice change during heat treatment. Often the residues of the previous cast are not disposed of but are reused and recycled until consumed. Thus, manufactured dental restorations have modified biological quality and chemical composition, and compromised biocompatibility. The aim of this study was to investigate the influence of repeated casting on the cytotoxicity of the silver-palladium (Ag-Pd) alloy. Our results showed that repeated casting of the Ag-Pd dental alloy affected its biocompatibility by promoting toxicity against transformed fibroblasts in a contact-independent manner. A strong decrease in cell proliferation, induction of senescence and massive cell death were observed in cultures exposed only to a medium previously incubated with dental alloy samples. The obtained data indicated that toxicity mediated by the accumulation of the Ag, Pd, Cu and Zn cations released from the Ag-Pd material was enhanced by recasting. The induction of cell senescence and subsequent apoptotic and necrotic death were accompanied by amplified intracellular production of reactive oxygen and nitrogen species, suggesting their involvement in the cell destruction process. Therefore, compromised biocompatibility after recasting with the Ag-Pd alloy can be the cause of serious local cell destruction, as observed in clinical practice
T2  - Archives of Biological Sciences
T1  - Recasting as a booster of Ag-Pd alloy cytotoxicity: Induction of cell senescence prior to mass cell death
IS  - 2
VL  - 71
DO  - 10.2298/ABS190305017C
SP  - 347
EP  - 356
ER  - 

@article{
author = "Čairović, Aleksandra and Stanimirović, Dragan and Krajnović, Tamara and Dojčinović, Biljana and Maksimović, Vesna and Cvijović-Alagić, Ivana",
year = "2019",
abstract = "The biological quality and chemical composition of alloys used in dental practice change during heat treatment. Often the residues of the previous cast are not disposed of but are reused and recycled until consumed. Thus, manufactured dental restorations have modified biological quality and chemical composition, and compromised biocompatibility. The aim of this study was to investigate the influence of repeated casting on the cytotoxicity of the silver-palladium (Ag-Pd) alloy. Our results showed that repeated casting of the Ag-Pd dental alloy affected its biocompatibility by promoting toxicity against transformed fibroblasts in a contact-independent manner. A strong decrease in cell proliferation, induction of senescence and massive cell death were observed in cultures exposed only to a medium previously incubated with dental alloy samples. The obtained data indicated that toxicity mediated by the accumulation of the Ag, Pd, Cu and Zn cations released from the Ag-Pd material was enhanced by recasting. The induction of cell senescence and subsequent apoptotic and necrotic death were accompanied by amplified intracellular production of reactive oxygen and nitrogen species, suggesting their involvement in the cell destruction process. Therefore, compromised biocompatibility after recasting with the Ag-Pd alloy can be the cause of serious local cell destruction, as observed in clinical practice",
journal = "Archives of Biological Sciences",
title = "Recasting as a booster of Ag-Pd alloy cytotoxicity: Induction of cell senescence prior to mass cell death",
number = "2",
volume = "71",
doi = "10.2298/ABS190305017C",
pages = "347-356"
}

Čairović, A., Stanimirović, D., Krajnović, T., Dojčinović, B., Maksimović, V.,& Cvijović-Alagić, I.. (2019). Recasting as a booster of Ag-Pd alloy cytotoxicity: Induction of cell senescence prior to mass cell death. in Archives of Biological Sciences, 71(2), 347-356.
https://doi.org/10.2298/ABS190305017C

Čairović A, Stanimirović D, Krajnović T, Dojčinović B, Maksimović V, Cvijović-Alagić I. Recasting as a booster of Ag-Pd alloy cytotoxicity: Induction of cell senescence prior to mass cell death. in Archives of Biological Sciences. 2019;71(2):347-356.
doi:10.2298/ABS190305017C .

Čairović, Aleksandra, Stanimirović, Dragan, Krajnović, Tamara, Dojčinović, Biljana, Maksimović, Vesna, Cvijović-Alagić, Ivana, "Recasting as a booster of Ag-Pd alloy cytotoxicity: Induction of cell senescence prior to mass cell death" in Archives of Biological Sciences, 71, no. 2 (2019):347-356,
https://doi.org/10.2298/ABS190305017C . .