TY  - JOUR
AU  - Ristić, Nataša
AU  - Ajdžanović, Vladimir
AU  - Manojlović-Stojanoski, Milica
AU  - Maliković, Jovana
AU  - Ušćebrka, Gordana
AU  - Marković, Zorica
AU  - Milošević, Verica
PY  - 2017
UR  - http://celljournal.org/journal/article/abstract/4334
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2846
AB  - Objective: Estrogen replacement therapy remains current as a therapeutic approach to treat menopausal symptoms and may significantly affect hormone-producing cells in the female pituitaries. The aim of this study was to examine the histological parameters of pituitary mammotrophs and prolactin secretion after chronic estradiol treatment in ovariectomized adult female rats, reflecting premature menopause. Materials and Methods: In this experimental study, adult female Wistar rats were divided into non-ovariectomized (C), ovariectomized (OVX) and estradiol-treated ovariectomized (OVX+E) groups. Estradiol dipropionate [0.625 mg/kg body mass per day] was administered for four weeks, while the C and OVX groups received vehicle alone. Mammotrophs were identified by the peroxidase-antiperoxidase (PAP) immunohistochemical procedure, while prolactin concentrations were measured by the non-isotopic two-step assay (Delfia) method. Comparison of the differences between groups was performed using one-way analysis of variance (ANOVA) and Tukay (honest significant difference) HSD test. Results: Ovariectomy caused significant (P < 0.05) decreases in mammotroph optical density (OD), volume density (V V ) and number per mm2 by 29, 27 and 34%, respectively, in comparison with the C females. In the OVX+E group, significant (P < 0.05) increases in OD, cell volume, V V and number of mammotrophs per mm2 by 181, 15%, 5.8-fold and 5.2-fold, respectively, were observed when compared to OVX animals. The serum prolactin concentration in OVX females was significantly (P < 0.05) decreased by 14% in comparison to the C group, while in OVX+E females, prolactin levels were significantly (P < 0.05) increased by 53% compared to the OVX controls. Conclusion: Estradiol supplementation in ovariectomized females is followed by stimulatory histological and secretory changes of the mammotrophs. These results could serve as indicators of possible prolactinome development upon estradiol application in premature menopausal subjects.
T2  - Cell Journal
T1  - Effects of estradiol on histological parameters and secretory ability of pituitary mammotrophs in ovariectomized female rats
IS  - 3
VL  - 19
DO  - 10.22074/cellj.2017.4334
SP  - 461
EP  - 468
ER  - 

@article{
author = "Ristić, Nataša and Ajdžanović, Vladimir and Manojlović-Stojanoski, Milica and Maliković, Jovana and Ušćebrka, Gordana and Marković, Zorica and Milošević, Verica",
year = "2017",
abstract = "Objective: Estrogen replacement therapy remains current as a therapeutic approach to treat menopausal symptoms and may significantly affect hormone-producing cells in the female pituitaries. The aim of this study was to examine the histological parameters of pituitary mammotrophs and prolactin secretion after chronic estradiol treatment in ovariectomized adult female rats, reflecting premature menopause. Materials and Methods: In this experimental study, adult female Wistar rats were divided into non-ovariectomized (C), ovariectomized (OVX) and estradiol-treated ovariectomized (OVX+E) groups. Estradiol dipropionate [0.625 mg/kg body mass per day] was administered for four weeks, while the C and OVX groups received vehicle alone. Mammotrophs were identified by the peroxidase-antiperoxidase (PAP) immunohistochemical procedure, while prolactin concentrations were measured by the non-isotopic two-step assay (Delfia) method. Comparison of the differences between groups was performed using one-way analysis of variance (ANOVA) and Tukay (honest significant difference) HSD test. Results: Ovariectomy caused significant (P < 0.05) decreases in mammotroph optical density (OD), volume density (V V ) and number per mm2 by 29, 27 and 34%, respectively, in comparison with the C females. In the OVX+E group, significant (P < 0.05) increases in OD, cell volume, V V and number of mammotrophs per mm2 by 181, 15%, 5.8-fold and 5.2-fold, respectively, were observed when compared to OVX animals. The serum prolactin concentration in OVX females was significantly (P < 0.05) decreased by 14% in comparison to the C group, while in OVX+E females, prolactin levels were significantly (P < 0.05) increased by 53% compared to the OVX controls. Conclusion: Estradiol supplementation in ovariectomized females is followed by stimulatory histological and secretory changes of the mammotrophs. These results could serve as indicators of possible prolactinome development upon estradiol application in premature menopausal subjects.",
journal = "Cell Journal",
title = "Effects of estradiol on histological parameters and secretory ability of pituitary mammotrophs in ovariectomized female rats",
number = "3",
volume = "19",
doi = "10.22074/cellj.2017.4334",
pages = "461-468"
}

Ristić, N., Ajdžanović, V., Manojlović-Stojanoski, M., Maliković, J., Ušćebrka, G., Marković, Z.,& Milošević, V.. (2017). Effects of estradiol on histological parameters and secretory ability of pituitary mammotrophs in ovariectomized female rats. in Cell Journal, 19(3), 461-468.
https://doi.org/10.22074/cellj.2017.4334

Ristić N, Ajdžanović V, Manojlović-Stojanoski M, Maliković J, Ušćebrka G, Marković Z, Milošević V. Effects of estradiol on histological parameters and secretory ability of pituitary mammotrophs in ovariectomized female rats. in Cell Journal. 2017;19(3):461-468.
doi:10.22074/cellj.2017.4334 .

Ristić, Nataša, Ajdžanović, Vladimir, Manojlović-Stojanoski, Milica, Maliković, Jovana, Ušćebrka, Gordana, Marković, Zorica, Milošević, Verica, "Effects of estradiol on histological parameters and secretory ability of pituitary mammotrophs in ovariectomized female rats" in Cell Journal, 19, no. 3 (2017):461-468,
https://doi.org/10.22074/cellj.2017.4334 . .