@conference{
editor = "Baebler, Špela, Dermastia, Marina, Grebenc, Tine, Praprotnik, Eva, Razinger, Jaka, Urbanek Krajnc, Andreja",
author = "Krstić-Milošević, Dijana and Banjac, Nevena and Janković, Teodora and Devrnja, Nina and Eler, Klemen and Vinterhalter, Branka",
year = "2022",
abstract = "Gentiana clusii Perr. & Song. (Gentianaceae), is a rare plant species found in the Alps, the Pyrenees, the Black Forest Mountains and the Carpathians. G. clusii is endemic for Europe which implies the need for its conservation. G. clusii plants were collected at fruitful stage in June 2015 at Velika Planina Mountain, Republic of Slovenia. Seeds of G. clusii were germinated on MS basal medium. Shoots cultured on WPM + 0.5 mg/L KIN were used as starting material in propagation and rooting experiments. Medium with IBA at 0.5 mg/L was chosen as optimal for rooting. HPLC screening of secondary metabolites of wild plants collected in nature and in vitro plants revealed the presence of two main classes of compounds typical for gentians – secoiridoids and xanthones. Secoiridoids gentiopicrin and sweroside were identified in both aerial parts of wild plants and shoots of in vitro plants, while root extracts contained amarogentin in addition. Aerial parts of wild plants contained gentiacaulein-1-O-primeveroside as dominant xanthone glycoside. HPLC also revealed the presence of gentiakochianin-1-O-primeveroside, norswertianin-1-O-primeveroside, 1,8- dihydroxy-3-methoxy-7-O-primeverosyl xanthone, and xanthone aglycons- norswertianin, gentiacaulein, and gentiakochianin. HPLC analysis shown that in vitro culture considerably promoted the production of secondary metabolites in G. clusii. The selected protocol for shoot propagation (WPM + 0.5 mg/L KIN) increased the content of sweroside, gentiopicrin and norswertianin-1-O-primeveroside for more than 2-fold compared with the wild plants. IBA promoted norswertianin-1-O-primeveroside and norswertianin production in root cultures; their contents were enhanced 6.4- and 18.6-fold, respectively, compared with the wild plants. The extract of these roots displayed high antioxidant capacity. The established shoot and root propagation protocols facilitate in vitro conservation of G. clusii, and provides a promising tool for the large scale production of valuable secoiridoids and xanthones.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia",
title = "Gentiana clusii Perr.&Song. cultured in vitro - secondary metabolite profile and evaluation of antioxidant activity",
pages = "31",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5560"
}
