TY  - JOUR
AU  - Marković, Zoran M.
AU  - Ristić, Biljana Z.
AU  - Arsikin, Katarina M.
AU  - Klisić, Đorđe G.
AU  - Harhaji-Trajković, Ljubica
AU  - Todorović-Marković, Biljana M.
AU  - Kepić, Dejan P.
AU  - Kravić-Stevović, Tamara K.
AU  - Jovanović, Svetlana P.
AU  - Milenković, Marina M.
AU  - Milivojević, Dusan D.
AU  - Bumbaširević, Vladimir Z.
AU  - Dramićanin, Miroslav D.
AU  - Trajković, Vladimir S.
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3587
AB  - The excellent photoluminescent properties of graphene quantum dots (GQD) makes them suitable candidates for biomedical applications, but their cytotoxicity has not been extensively studied. Here we show that electrochemically produced GQD irradiated with blue light (470. nm, 1. W) generate reactive oxygen species, including singlet oxygen, and kill U251 human glioma cells by causing oxidative stress. The cell death induced by photoexcited GQD displayed morphological and/or biochemical characteristics of both apoptosis (phosphatidylserine externalization, caspase activation, DNA fragmentation) and autophagy (formation of autophagic vesicles, LC3-I/LC3-II conversion, degradation of autophagic target p62). Moreover, a genetic inactivation of autophagy-essential LC3B protein partly abrogated the photodynamic cytotoxicity of GQD. These data indicate potential usefulness of GQD in photodynamic therapy, but also raise concerns about their possible toxicity.
PB  - Elsevier BV
T2  - Biomaterials
T1  - Graphene quantum dots as autophagy-inducing photodynamic agents
IS  - 29
VL  - 33
DO  - 10.1016/j.biomaterials.2012.06.060
SP  - 7084
EP  - 7092
ER  - 

@article{
author = "Marković, Zoran M. and Ristić, Biljana Z. and Arsikin, Katarina M. and Klisić, Đorđe G. and Harhaji-Trajković, Ljubica and Todorović-Marković, Biljana M. and Kepić, Dejan P. and Kravić-Stevović, Tamara K. and Jovanović, Svetlana P. and Milenković, Marina M. and Milivojević, Dusan D. and Bumbaširević, Vladimir Z. and Dramićanin, Miroslav D. and Trajković, Vladimir S.",
year = "2012",
abstract = "The excellent photoluminescent properties of graphene quantum dots (GQD) makes them suitable candidates for biomedical applications, but their cytotoxicity has not been extensively studied. Here we show that electrochemically produced GQD irradiated with blue light (470. nm, 1. W) generate reactive oxygen species, including singlet oxygen, and kill U251 human glioma cells by causing oxidative stress. The cell death induced by photoexcited GQD displayed morphological and/or biochemical characteristics of both apoptosis (phosphatidylserine externalization, caspase activation, DNA fragmentation) and autophagy (formation of autophagic vesicles, LC3-I/LC3-II conversion, degradation of autophagic target p62). Moreover, a genetic inactivation of autophagy-essential LC3B protein partly abrogated the photodynamic cytotoxicity of GQD. These data indicate potential usefulness of GQD in photodynamic therapy, but also raise concerns about their possible toxicity.",
publisher = "Elsevier BV",
journal = "Biomaterials",
title = "Graphene quantum dots as autophagy-inducing photodynamic agents",
number = "29",
volume = "33",
doi = "10.1016/j.biomaterials.2012.06.060",
pages = "7084-7092"
}

Marković, Z. M., Ristić, B. Z., Arsikin, K. M., Klisić, Đ. G., Harhaji-Trajković, L., Todorović-Marković, B. M., Kepić, D. P., Kravić-Stevović, T. K., Jovanović, S. P., Milenković, M. M., Milivojević, D. D., Bumbaširević, V. Z., Dramićanin, M. D.,& Trajković, V. S.. (2012). Graphene quantum dots as autophagy-inducing photodynamic agents. in Biomaterials
Elsevier BV., 33(29), 7084-7092.
https://doi.org/10.1016/j.biomaterials.2012.06.060

Marković ZM, Ristić BZ, Arsikin KM, Klisić ĐG, Harhaji-Trajković L, Todorović-Marković BM, Kepić DP, Kravić-Stevović TK, Jovanović SP, Milenković MM, Milivojević DD, Bumbaširević VZ, Dramićanin MD, Trajković VS. Graphene quantum dots as autophagy-inducing photodynamic agents. in Biomaterials. 2012;33(29):7084-7092.
doi:10.1016/j.biomaterials.2012.06.060 .

Marković, Zoran M., Ristić, Biljana Z., Arsikin, Katarina M., Klisić, Đorđe G., Harhaji-Trajković, Ljubica, Todorović-Marković, Biljana M., Kepić, Dejan P., Kravić-Stevović, Tamara K., Jovanović, Svetlana P., Milenković, Marina M., Milivojević, Dusan D., Bumbaširević, Vladimir Z., Dramićanin, Miroslav D., Trajković, Vladimir S., "Graphene quantum dots as autophagy-inducing photodynamic agents" in Biomaterials, 33, no. 29 (2012):7084-7092,
https://doi.org/10.1016/j.biomaterials.2012.06.060 . .