TY  - JOUR
AU  - Pavić, Aleksandar
AU  - Ilić-Tomić, Tatjana
AU  - Glamočlija, Jasmina
PY  - 2021
UR  - https://www.mdpi.com/2309-608X/7/10/834
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8540621
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4634
AB  - Severe drawbacks associated with the topical use of depigmenting agents in treatments of skin hyperigmentations impose a great demand for novel, effective, and safe melanogenesis inhibitors. Edible and medicinal mushrooms, known for numerous health-promoting properties, represent a rich reservoir of anti-melanogenic compounds, with the potential to be applied in preventing excessive skin pigmentation. Herein, using zebrafish (Danio rerio) as a preclinical animal model, we have demonstrated that ethanol extract of Laetiporus sulphureus (LSE) and Agaricus silvaticus (ASE) are not toxic at high doses up to 400-500 µg/mL while effectively inhibit melanogenesis in a dose-dependent manner. At depigmenting doses, the explored extracts showed no adverse effects on zebrafish embryos melanocytes. Even more, they did not provoke inflammation or neutropenia when applied at the highest dose ensuring almost complete the cells depigmentation. Since LSE and ASE have demonstrated significantly higher the therapeutic potential than kojic acid and hydroquinone, two well-known depigmenting agents, overall results of this study strongly suggest that the explored mushrooms extracts could be used as efficient and safe topical agents in treatments of skin hyperpigmentation disorders.
PB  - Basel: MDPI
T2  - Journal of Fungi
T1  - Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model.
IS  - 10
VL  - 7
DO  - 10.3390/jof7100834
SP  - 834
ER  - 

@article{
author = "Pavić, Aleksandar and Ilić-Tomić, Tatjana and Glamočlija, Jasmina",
year = "2021",
abstract = "Severe drawbacks associated with the topical use of depigmenting agents in treatments of skin hyperigmentations impose a great demand for novel, effective, and safe melanogenesis inhibitors. Edible and medicinal mushrooms, known for numerous health-promoting properties, represent a rich reservoir of anti-melanogenic compounds, with the potential to be applied in preventing excessive skin pigmentation. Herein, using zebrafish (Danio rerio) as a preclinical animal model, we have demonstrated that ethanol extract of Laetiporus sulphureus (LSE) and Agaricus silvaticus (ASE) are not toxic at high doses up to 400-500 µg/mL while effectively inhibit melanogenesis in a dose-dependent manner. At depigmenting doses, the explored extracts showed no adverse effects on zebrafish embryos melanocytes. Even more, they did not provoke inflammation or neutropenia when applied at the highest dose ensuring almost complete the cells depigmentation. Since LSE and ASE have demonstrated significantly higher the therapeutic potential than kojic acid and hydroquinone, two well-known depigmenting agents, overall results of this study strongly suggest that the explored mushrooms extracts could be used as efficient and safe topical agents in treatments of skin hyperpigmentation disorders.",
publisher = "Basel: MDPI",
journal = "Journal of Fungi",
title = "Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model.",
number = "10",
volume = "7",
doi = "10.3390/jof7100834",
pages = "834"
}

Pavić, A., Ilić-Tomić, T.,& Glamočlija, J.. (2021). Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model.. in Journal of Fungi
Basel: MDPI., 7(10), 834.
https://doi.org/10.3390/jof7100834

Pavić A, Ilić-Tomić T, Glamočlija J. Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model.. in Journal of Fungi. 2021;7(10):834.
doi:10.3390/jof7100834 .

Pavić, Aleksandar, Ilić-Tomić, Tatjana, Glamočlija, Jasmina, "Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model." in Journal of Fungi, 7, no. 10 (2021):834,
https://doi.org/10.3390/jof7100834 . .

TY  - CONF
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Ilić-Tomić, Tatjana
AU  - Soković, Marina
AU  - Robajac, Dragana
AU  - Nedić, Olgica
AU  - Pavić, Aleksandar
PY  - 2020
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6535
AB  - Laetiporus sulphureus, an edible mushroom, for centuries has been used in traditional 
European and Asian ethno-medicine. Its therapeutic properties have been attributed to different 
types of biologically active compounds, such as terpenes, polysaccharides, steroids, proteins 
and lectins. Recently, mushroom lectins emerged as very potent macromolecules with 
antiproliferative, cytotoxic, antiviral and immunostimulatory activities. Therefore, we isolated 
lectin from L. sulphureus (LSL) and evaluated its anti-angiogenic and anticancer activity in 
vivo, using the zebrafish model. We found that LSL is not toxic at high doses up to 400-
500 μg/mL, while it effectively inhibited angiogenesis and cancer development at much lower 
doses. Compared to sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of 
clinical relevance, therapeutic potential of LSL was 378-fold higher. Wound healing and MTT 
assays were employed to examine antimigratory effect and endothelial cytotoxicity. 
Surprisingly, LSL affected human colorectal carcinoma and mouse melanoma cell lines, by 
almost completely diminishing their growth, neovascularization and metastasis. Moreover, in 
comparison to the used control (cisplatin), LSL showed markedly greater activity, while its 
potency turned out to be 8-fold higher towards colorectal carcinoma than melanoma. These 
encouraging data strongly imply that LSL can be considered for the application as supporting 
agent in chemotherapy against colorectal carcinoma and melanoma or used in pharmaceutical 
industry.
PB  - Belgrade: NutRedox, COST Action CA16112
C3  - WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia
T1  - Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6535
ER  - 

@conference{
author = "Petrović, Jovana and Glamočlija, Jasmina and Ilić-Tomić, Tatjana and Soković, Marina and Robajac, Dragana and Nedić, Olgica and Pavić, Aleksandar",
year = "2020",
abstract = "Laetiporus sulphureus, an edible mushroom, for centuries has been used in traditional 
European and Asian ethno-medicine. Its therapeutic properties have been attributed to different 
types of biologically active compounds, such as terpenes, polysaccharides, steroids, proteins 
and lectins. Recently, mushroom lectins emerged as very potent macromolecules with 
antiproliferative, cytotoxic, antiviral and immunostimulatory activities. Therefore, we isolated 
lectin from L. sulphureus (LSL) and evaluated its anti-angiogenic and anticancer activity in 
vivo, using the zebrafish model. We found that LSL is not toxic at high doses up to 400-
500 μg/mL, while it effectively inhibited angiogenesis and cancer development at much lower 
doses. Compared to sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of 
clinical relevance, therapeutic potential of LSL was 378-fold higher. Wound healing and MTT 
assays were employed to examine antimigratory effect and endothelial cytotoxicity. 
Surprisingly, LSL affected human colorectal carcinoma and mouse melanoma cell lines, by 
almost completely diminishing their growth, neovascularization and metastasis. Moreover, in 
comparison to the used control (cisplatin), LSL showed markedly greater activity, while its 
potency turned out to be 8-fold higher towards colorectal carcinoma than melanoma. These 
encouraging data strongly imply that LSL can be considered for the application as supporting 
agent in chemotherapy against colorectal carcinoma and melanoma or used in pharmaceutical 
industry.",
publisher = "Belgrade: NutRedox, COST Action CA16112",
journal = "WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia",
title = "Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6535"
}

Petrović, J., Glamočlija, J., Ilić-Tomić, T., Soković, M., Robajac, D., Nedić, O.,& Pavić, A.. (2020). Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.. in WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia
Belgrade: NutRedox, COST Action CA16112., 43.
https://hdl.handle.net/21.15107/rcub_ibiss_6535

Petrović J, Glamočlija J, Ilić-Tomić T, Soković M, Robajac D, Nedić O, Pavić A. Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.. in WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia. 2020;:43.
https://hdl.handle.net/21.15107/rcub_ibiss_6535 .

Petrović, Jovana, Glamočlija, Jasmina, Ilić-Tomić, Tatjana, Soković, Marina, Robajac, Dragana, Nedić, Olgica, Pavić, Aleksandar, "Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma." in WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia (2020):43,
https://hdl.handle.net/21.15107/rcub_ibiss_6535 .