TY  - CONF
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Ilić-Tomić, Tatjana
AU  - Soković, Marina
AU  - Robajac, Dragana
AU  - Nedić, Olgica
AU  - Pavić, Aleksandar
PY  - 2020
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6535
AB  - Laetiporus sulphureus, an edible mushroom, for centuries has been used in traditional 
European and Asian ethno-medicine. Its therapeutic properties have been attributed to different 
types of biologically active compounds, such as terpenes, polysaccharides, steroids, proteins 
and lectins. Recently, mushroom lectins emerged as very potent macromolecules with 
antiproliferative, cytotoxic, antiviral and immunostimulatory activities. Therefore, we isolated 
lectin from L. sulphureus (LSL) and evaluated its anti-angiogenic and anticancer activity in 
vivo, using the zebrafish model. We found that LSL is not toxic at high doses up to 400-
500 μg/mL, while it effectively inhibited angiogenesis and cancer development at much lower 
doses. Compared to sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of 
clinical relevance, therapeutic potential of LSL was 378-fold higher. Wound healing and MTT 
assays were employed to examine antimigratory effect and endothelial cytotoxicity. 
Surprisingly, LSL affected human colorectal carcinoma and mouse melanoma cell lines, by 
almost completely diminishing their growth, neovascularization and metastasis. Moreover, in 
comparison to the used control (cisplatin), LSL showed markedly greater activity, while its 
potency turned out to be 8-fold higher towards colorectal carcinoma than melanoma. These 
encouraging data strongly imply that LSL can be considered for the application as supporting 
agent in chemotherapy against colorectal carcinoma and melanoma or used in pharmaceutical 
industry.
PB  - Belgrade: NutRedox, COST Action CA16112
C3  - WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia
T1  - Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6535
ER  - 

@conference{
author = "Petrović, Jovana and Glamočlija, Jasmina and Ilić-Tomić, Tatjana and Soković, Marina and Robajac, Dragana and Nedić, Olgica and Pavić, Aleksandar",
year = "2020",
abstract = "Laetiporus sulphureus, an edible mushroom, for centuries has been used in traditional 
European and Asian ethno-medicine. Its therapeutic properties have been attributed to different 
types of biologically active compounds, such as terpenes, polysaccharides, steroids, proteins 
and lectins. Recently, mushroom lectins emerged as very potent macromolecules with 
antiproliferative, cytotoxic, antiviral and immunostimulatory activities. Therefore, we isolated 
lectin from L. sulphureus (LSL) and evaluated its anti-angiogenic and anticancer activity in 
vivo, using the zebrafish model. We found that LSL is not toxic at high doses up to 400-
500 μg/mL, while it effectively inhibited angiogenesis and cancer development at much lower 
doses. Compared to sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of 
clinical relevance, therapeutic potential of LSL was 378-fold higher. Wound healing and MTT 
assays were employed to examine antimigratory effect and endothelial cytotoxicity. 
Surprisingly, LSL affected human colorectal carcinoma and mouse melanoma cell lines, by 
almost completely diminishing their growth, neovascularization and metastasis. Moreover, in 
comparison to the used control (cisplatin), LSL showed markedly greater activity, while its 
potency turned out to be 8-fold higher towards colorectal carcinoma than melanoma. These 
encouraging data strongly imply that LSL can be considered for the application as supporting 
agent in chemotherapy against colorectal carcinoma and melanoma or used in pharmaceutical 
industry.",
publisher = "Belgrade: NutRedox, COST Action CA16112",
journal = "WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia",
title = "Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6535"
}

Petrović, J., Glamočlija, J., Ilić-Tomić, T., Soković, M., Robajac, D., Nedić, O.,& Pavić, A.. (2020). Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.. in WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia
Belgrade: NutRedox, COST Action CA16112., 43.
https://hdl.handle.net/21.15107/rcub_ibiss_6535

Petrović J, Glamočlija J, Ilić-Tomić T, Soković M, Robajac D, Nedić O, Pavić A. Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.. in WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia. 2020;:43.
https://hdl.handle.net/21.15107/rcub_ibiss_6535 .

Petrović, Jovana, Glamočlija, Jasmina, Ilić-Tomić, Tatjana, Soković, Marina, Robajac, Dragana, Nedić, Olgica, Pavić, Aleksandar, "Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma." in WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia (2020):43,
https://hdl.handle.net/21.15107/rcub_ibiss_6535 .

TY  - JOUR
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Ilić-Tomić, Tatjana
AU  - Soković, Marina
AU  - Robajac, Dragana
AU  - Nedić, Olgica
AU  - Pavić, Aleksandar
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3603
AB  - In spite of extensive usage of Laetiporus sulphureus (sulphur polypore) in traditional European and Asian ethno-medicine for centuries, its anticancer therapeutic potential and toxicity profile remained explored in animal models. Herein, using zebrafish (Danio rerio), as a preclinical animal model, we demonstrated that L. sulphureus lectin (LSL) and ethanol extract (LSE) are non-toxic at high doses up to 400-500 μg/mL, while they effectively inhibited angiogenesis and cancer development at much lower doses. Lectin showed 74-fold higher anti-angiogenic potency than the extract, and even 378-fold higher therapeutic potential than sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of clinical relevance. Using wound healing and MTT assays, we proved LSL's strong antimigratory effect and selective endothelial cytotoxicity in relation to lung fibroblasts. In addition, employing the zebrafish xenograft models, we demonstrated that LSL almost completely reduced growth, neovascularization and metastasis of human colorectal carcinoma and mouse melanoma. Even more, LSL exerted 8-fold higher potency towards colorectal carcinoma than melanoma, showing markedly higher activity than cisplatin, while LSE failed to express any anticancer activity. Accompanied with non-toxic response, including neutropenia and inflammation, the results of this study strongly imply that LSL could be used as safe adjuvant in chemotherapy against colorectal carcinoma and melanoma.
T2  - International Journal of Biological Macromolecules
T1  - Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.
VL  - 148
DO  - 10.1016/j.ijbiomac.2020.01.033
SP  - 129
EP  - 139
ER  - 

@article{
author = "Petrović, Jovana and Glamočlija, Jasmina and Ilić-Tomić, Tatjana and Soković, Marina and Robajac, Dragana and Nedić, Olgica and Pavić, Aleksandar",
year = "2020",
abstract = "In spite of extensive usage of Laetiporus sulphureus (sulphur polypore) in traditional European and Asian ethno-medicine for centuries, its anticancer therapeutic potential and toxicity profile remained explored in animal models. Herein, using zebrafish (Danio rerio), as a preclinical animal model, we demonstrated that L. sulphureus lectin (LSL) and ethanol extract (LSE) are non-toxic at high doses up to 400-500 μg/mL, while they effectively inhibited angiogenesis and cancer development at much lower doses. Lectin showed 74-fold higher anti-angiogenic potency than the extract, and even 378-fold higher therapeutic potential than sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of clinical relevance. Using wound healing and MTT assays, we proved LSL's strong antimigratory effect and selective endothelial cytotoxicity in relation to lung fibroblasts. In addition, employing the zebrafish xenograft models, we demonstrated that LSL almost completely reduced growth, neovascularization and metastasis of human colorectal carcinoma and mouse melanoma. Even more, LSL exerted 8-fold higher potency towards colorectal carcinoma than melanoma, showing markedly higher activity than cisplatin, while LSE failed to express any anticancer activity. Accompanied with non-toxic response, including neutropenia and inflammation, the results of this study strongly imply that LSL could be used as safe adjuvant in chemotherapy against colorectal carcinoma and melanoma.",
journal = "International Journal of Biological Macromolecules",
title = "Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.",
volume = "148",
doi = "10.1016/j.ijbiomac.2020.01.033",
pages = "129-139"
}

Petrović, J., Glamočlija, J., Ilić-Tomić, T., Soković, M., Robajac, D., Nedić, O.,& Pavić, A.. (2020). Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.. in International Journal of Biological Macromolecules, 148, 129-139.
https://doi.org/10.1016/j.ijbiomac.2020.01.033

Petrović J, Glamočlija J, Ilić-Tomić T, Soković M, Robajac D, Nedić O, Pavić A. Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.. in International Journal of Biological Macromolecules. 2020;148:129-139.
doi:10.1016/j.ijbiomac.2020.01.033 .

Petrović, Jovana, Glamočlija, Jasmina, Ilić-Tomić, Tatjana, Soković, Marina, Robajac, Dragana, Nedić, Olgica, Pavić, Aleksandar, "Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma." in International Journal of Biological Macromolecules, 148 (2020):129-139,
https://doi.org/10.1016/j.ijbiomac.2020.01.033 . .

TY  - CONF
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Robajac, Dragana
AU  - Nedić, Olgica
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6539
AB  - Lektini su proteini koji specifično prepoznaju ugljene hidrate i imaju brojne biološke 
aktivnosti. Poznato je da gljive (makromicete, mikromicete i kvasci) predstavljaju bogat 
izvor lektina. Iako prema objavljenim podacima bazidiokarpi Laetiporus sulphureus 
predstavljaju značajan izvor lektina izražene hemolitičke aktivnosti1
, lektini autohtonog 
soja ove vrste sa teritorije Srbije nisu do sada izolovani i okarakterisani. Uzorak 
plodonosnog tela sakupljen u okolini Beograda je zamrznut i homogenizovan blenderom 
u PBSE puferu. Nakon prekonoćne ekstrakcije na 4°C uzorak je centrifugiran i za dalju 
analizu korišćen je supernatant. Precipitacija je izvedena dodavanjem amonijum sulfata 
do 80% zasićenja, nakon čega je smeša centrifugirana, a pelet koji je sadržao lektin 
resuspendovan u PBSE. Prisustvo lektina potvrđeno je reakcijom hemaglutinacije sa 
humanim eritrocitima, dok je njegovo prečišćavanje ostvareno korišćenjem Sefaroze CL 6B kao afinitetnog matriksa. Stepen čistoće i aktivnost lektina potvrđeni su nativnom 
elektroforezom i transferom na nitroceluloznu membranu koja je potom inkubirana sa 
tripsinizovanim humanim eritrocitima.2
Lektin je vizuelizovan na membrani kao crvena 
traka poreklom od aglutiniranih eritrocita. Intenzitet trake, kao i hemoliza eritrocita 
ukazali su ne samo na aktivnost već i značajnu zastupljenost lektina u bazidiokarpima L. 
sulphureus. Prinos čistog jedinjenja je iznosio 15 mg/100 g plodonosnog tela, čak 
dvostruko više od vrednosti koja je dobijena za uzorak ove vrste sa lokaliteta SAD.
PB  - Belgrade: Serbian Biological Socitey
C3  - Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
T1  - Izolacija lektina iz bazidiokarpa autohtonog soja Laetiporus sulphureus (Bull.: Fr.) Murr
SP  - 217
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6539
ER  - 

@conference{
author = "Petrović, Jovana and Glamočlija, Jasmina and Soković, Marina and Robajac, Dragana and Nedić, Olgica",
year = "2018",
abstract = "Lektini su proteini koji specifično prepoznaju ugljene hidrate i imaju brojne biološke 
aktivnosti. Poznato je da gljive (makromicete, mikromicete i kvasci) predstavljaju bogat 
izvor lektina. Iako prema objavljenim podacima bazidiokarpi Laetiporus sulphureus 
predstavljaju značajan izvor lektina izražene hemolitičke aktivnosti1
, lektini autohtonog 
soja ove vrste sa teritorije Srbije nisu do sada izolovani i okarakterisani. Uzorak 
plodonosnog tela sakupljen u okolini Beograda je zamrznut i homogenizovan blenderom 
u PBSE puferu. Nakon prekonoćne ekstrakcije na 4°C uzorak je centrifugiran i za dalju 
analizu korišćen je supernatant. Precipitacija je izvedena dodavanjem amonijum sulfata 
do 80% zasićenja, nakon čega je smeša centrifugirana, a pelet koji je sadržao lektin 
resuspendovan u PBSE. Prisustvo lektina potvrđeno je reakcijom hemaglutinacije sa 
humanim eritrocitima, dok je njegovo prečišćavanje ostvareno korišćenjem Sefaroze CL 6B kao afinitetnog matriksa. Stepen čistoće i aktivnost lektina potvrđeni su nativnom 
elektroforezom i transferom na nitroceluloznu membranu koja je potom inkubirana sa 
tripsinizovanim humanim eritrocitima.2
Lektin je vizuelizovan na membrani kao crvena 
traka poreklom od aglutiniranih eritrocita. Intenzitet trake, kao i hemoliza eritrocita 
ukazali su ne samo na aktivnost već i značajnu zastupljenost lektina u bazidiokarpima L. 
sulphureus. Prinos čistog jedinjenja je iznosio 15 mg/100 g plodonosnog tela, čak 
dvostruko više od vrednosti koja je dobijena za uzorak ove vrste sa lokaliteta SAD.",
publisher = "Belgrade: Serbian Biological Socitey",
journal = "Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija",
title = "Izolacija lektina iz bazidiokarpa autohtonog soja Laetiporus sulphureus (Bull.: Fr.) Murr",
pages = "217",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6539"
}

Petrović, J., Glamočlija, J., Soković, M., Robajac, D.,& Nedić, O.. (2018). Izolacija lektina iz bazidiokarpa autohtonog soja Laetiporus sulphureus (Bull.: Fr.) Murr. in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
Belgrade: Serbian Biological Socitey., 217.
https://hdl.handle.net/21.15107/rcub_ibiss_6539

Petrović J, Glamočlija J, Soković M, Robajac D, Nedić O. Izolacija lektina iz bazidiokarpa autohtonog soja Laetiporus sulphureus (Bull.: Fr.) Murr. in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija. 2018;:217.
https://hdl.handle.net/21.15107/rcub_ibiss_6539 .

Petrović, Jovana, Glamočlija, Jasmina, Soković, Marina, Robajac, Dragana, Nedić, Olgica, "Izolacija lektina iz bazidiokarpa autohtonog soja Laetiporus sulphureus (Bull.: Fr.) Murr" in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija (2018):217,
https://hdl.handle.net/21.15107/rcub_ibiss_6539 .