TY  - JOUR
AU  - Veličković, Ivona
AU  - Žižak, Željko
AU  - Simin, Nataša
AU  - Bekvalac, Kristina
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Marin, Petar D.
AU  - Grujić, Slavica
PY  - 2021
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=1821-21582102215V
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4690
AB  - The berries of Rubus discolor are considered a rich source of phytochemicals which could play an important role in the prevention of prevalent contemporary chronic diseases. Thus, the goal of the presented study was to determine the profile of phenolic acids and anthocyanins of aqueous and ethanol extracts of R. discolor fruit, and their free radical scavenging, antiproliferative, antidiabetic and antimicrobial activities. LC-MS/MS analyses confirmed the presence of 11 phenolic acids with protocatechuic and gallic acids being the major compounds. Additionally, cyanidin-glucoside/galactoside was the most abundant among the five identified anthocyanins. The ethanol extract was more efficient in scavenging free radicals than the aqueous extract. The absence of antiproliferative activity was observed for both extracts. However, they inhibited carbohydrate hydrolysing digestive enzymes associated with type-2 diabetes. Furthermore, the obtained results for α-glucosidase inhibitory activity (IC50 values 44.52 and 80.72 µg/mL, for the aqueous and ethanol extracts, respectively) indicate significantly higher activity than the positive control, Glucobay® (233.38 µg/mL). The ethanol extract was more effective against all of the examined bacteria (Bacillus cereus, Staphylococcus aureus and Enterobacter cloacae) than the aqueous extract. On the contrary, the aqueous extract showed better antifungal properties, particularly against Trihoderma viride and Penicillium verrucosum var. cyclopium. The quantified phenolics and presented bioactivities of R. discolor fruit extracts candidate them as a potential source of bioactive compounds which might be used in the food, pharmacy and cosmetic industries.
PB  - Belgrade: Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica
T1  - Phenolic profile and biological potential of wild blackberry (Rubus discolor) fruits
IS  - 2
VL  - 45
DO  - 10.2298/BOTSERB2102215V
SP  - 215
EP  - 222
ER  - 

@article{
author = "Veličković, Ivona and Žižak, Željko and Simin, Nataša and Bekvalac, Kristina and Ivanov, Marija and Soković, Marina and Marin, Petar D. and Grujić, Slavica",
year = "2021",
abstract = "The berries of Rubus discolor are considered a rich source of phytochemicals which could play an important role in the prevention of prevalent contemporary chronic diseases. Thus, the goal of the presented study was to determine the profile of phenolic acids and anthocyanins of aqueous and ethanol extracts of R. discolor fruit, and their free radical scavenging, antiproliferative, antidiabetic and antimicrobial activities. LC-MS/MS analyses confirmed the presence of 11 phenolic acids with protocatechuic and gallic acids being the major compounds. Additionally, cyanidin-glucoside/galactoside was the most abundant among the five identified anthocyanins. The ethanol extract was more efficient in scavenging free radicals than the aqueous extract. The absence of antiproliferative activity was observed for both extracts. However, they inhibited carbohydrate hydrolysing digestive enzymes associated with type-2 diabetes. Furthermore, the obtained results for α-glucosidase inhibitory activity (IC50 values 44.52 and 80.72 µg/mL, for the aqueous and ethanol extracts, respectively) indicate significantly higher activity than the positive control, Glucobay® (233.38 µg/mL). The ethanol extract was more effective against all of the examined bacteria (Bacillus cereus, Staphylococcus aureus and Enterobacter cloacae) than the aqueous extract. On the contrary, the aqueous extract showed better antifungal properties, particularly against Trihoderma viride and Penicillium verrucosum var. cyclopium. The quantified phenolics and presented bioactivities of R. discolor fruit extracts candidate them as a potential source of bioactive compounds which might be used in the food, pharmacy and cosmetic industries.",
publisher = "Belgrade: Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica",
title = "Phenolic profile and biological potential of wild blackberry (Rubus discolor) fruits",
number = "2",
volume = "45",
doi = "10.2298/BOTSERB2102215V",
pages = "215-222"
}

Veličković, I., Žižak, Ž., Simin, N., Bekvalac, K., Ivanov, M., Soković, M., Marin, P. D.,& Grujić, S.. (2021). Phenolic profile and biological potential of wild blackberry (Rubus discolor) fruits. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden Jevremovac., 45(2), 215-222.
https://doi.org/10.2298/BOTSERB2102215V

Veličković I, Žižak Ž, Simin N, Bekvalac K, Ivanov M, Soković M, Marin PD, Grujić S. Phenolic profile and biological potential of wild blackberry (Rubus discolor) fruits. in Botanica Serbica. 2021;45(2):215-222.
doi:10.2298/BOTSERB2102215V .

Veličković, Ivona, Žižak, Željko, Simin, Nataša, Bekvalac, Kristina, Ivanov, Marija, Soković, Marina, Marin, Petar D., Grujić, Slavica, "Phenolic profile and biological potential of wild blackberry (Rubus discolor) fruits" in Botanica Serbica, 45, no. 2 (2021):215-222,
https://doi.org/10.2298/BOTSERB2102215V . .

TY  - JOUR
AU  - Veličković, Ivona
AU  - Žižak, Željko
AU  - Rajčević, Nemanja
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Marin, Petar
AU  - Grujić, Slavica
PY  - 2021
UR  - https://www.notulaebotanicae.ro/index.php/nbha/article/view/12137
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4213
AB  - Prunus spinosa leaf extracts in solvents of different polarity (water, ethanol and acetone), their phenol, flavonoid and anthocyanin contents and biological properties were the object of this study. The richest in phenols as well as in flavonoids was acetone extract with 181.19 mg GAE and 80.10 mg QE per gram of dry extract, respectively. Moreover, the quantity of anthocyanins obtained by HPLC analysis was also the highest in acetone sample. Examined samples possessed antioxidant properties evaluated through four in vitro assays (DPPH, ABTS, FRAP and TRC). The acetone extract was proved to be the best antioxidant among tested samples, which could be ascribed to polyphenols, especially anthocyanins. The aqueous and the ethanol extract exhibited antibacterial effects, being particularly active against B. cereus and E. cloacae. T. viride, P. funiculosum, P. ochrochloron, P. verrucosum var. cyclopium were the most susceptible among fungal microorganisms examined. Both, the aqueous and the ethanol extract expressed inhibitory activity towards enzymes linked to diabetes mellitus type II. Additionally, the ethanol extract showed significantly higher potential in inhibiting α-glucosidase than the drug used as the positive control. Furthermore, the aqueous sample revealed antitumor effects on following malignant cell lines: HeLa, K562 and MDA-MB-453. The results presented herein suggest that P. spinosa leaves should be considered as a natural source of bioactive compounds with potential application in phytopharmacy and food industry.
PB  - University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
T2  - Notulae Botanicae Horti Agrobotanici Cluj-Napoca
T1  - Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities
IS  - 1
VL  - 49
DO  - 10.15835/nbha49112137
SP  - 12137
ER  - 

@article{
author = "Veličković, Ivona and Žižak, Željko and Rajčević, Nemanja and Ivanov, Marija and Soković, Marina and Marin, Petar and Grujić, Slavica",
year = "2021",
abstract = "Prunus spinosa leaf extracts in solvents of different polarity (water, ethanol and acetone), their phenol, flavonoid and anthocyanin contents and biological properties were the object of this study. The richest in phenols as well as in flavonoids was acetone extract with 181.19 mg GAE and 80.10 mg QE per gram of dry extract, respectively. Moreover, the quantity of anthocyanins obtained by HPLC analysis was also the highest in acetone sample. Examined samples possessed antioxidant properties evaluated through four in vitro assays (DPPH, ABTS, FRAP and TRC). The acetone extract was proved to be the best antioxidant among tested samples, which could be ascribed to polyphenols, especially anthocyanins. The aqueous and the ethanol extract exhibited antibacterial effects, being particularly active against B. cereus and E. cloacae. T. viride, P. funiculosum, P. ochrochloron, P. verrucosum var. cyclopium were the most susceptible among fungal microorganisms examined. Both, the aqueous and the ethanol extract expressed inhibitory activity towards enzymes linked to diabetes mellitus type II. Additionally, the ethanol extract showed significantly higher potential in inhibiting α-glucosidase than the drug used as the positive control. Furthermore, the aqueous sample revealed antitumor effects on following malignant cell lines: HeLa, K562 and MDA-MB-453. The results presented herein suggest that P. spinosa leaves should be considered as a natural source of bioactive compounds with potential application in phytopharmacy and food industry.",
publisher = "University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca",
journal = "Notulae Botanicae Horti Agrobotanici Cluj-Napoca",
title = "Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities",
number = "1",
volume = "49",
doi = "10.15835/nbha49112137",
pages = "12137"
}

Veličković, I., Žižak, Ž., Rajčević, N., Ivanov, M., Soković, M., Marin, P.,& Grujić, S.. (2021). Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca
University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca., 49(1), 12137.
https://doi.org/10.15835/nbha49112137

Veličković I, Žižak Ž, Rajčević N, Ivanov M, Soković M, Marin P, Grujić S. Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2021;49(1):12137.
doi:10.15835/nbha49112137 .

Veličković, Ivona, Žižak, Željko, Rajčević, Nemanja, Ivanov, Marija, Soković, Marina, Marin, Petar, Grujić, Slavica, "Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities" in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49, no. 1 (2021):12137,
https://doi.org/10.15835/nbha49112137 . .

TY  - JOUR
AU  - Veličković, Ivona
AU  - Žižak, Željko
AU  - Rajčević, Nemanja
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Marin, Petar
AU  - Grujić, Slavica
PY  - 2020
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4932
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3652
AB  - We investigated the total phenolic and flavonoid contents and the anthocyanin profiles in aqueous, ethanol and acetone extracts of Prunus spinosa (Rosaceae) fruit, and their antioxidant, antibacterial, antifungal, antidiabetic and antitumor properties. The contribution of polyphenol contents to the bioactivity of the extracts was calculated and observed through Pearson’s coefficient of correlation. The acetone extract was the richest in phenols and anthocyanins and the ethanol extract in flavonoids. Cyanidin was the most abundant anthocyanin compound in all examined extracts. The ethanol extract showed the most promising antioxidant activity in DPPH, ABTS and FRAP assays. Tested bacteria were more affected by the ethanol than by the aqueous extract. Both the ethanol and aqueous extracts exhibited potential antidiabetic effects, observed as inhibition of α-amylase and α-glucosidase, enzymes linked with diabetes mellitus type II. The ethanol extract was a potent α-glucosidase-inhibitor with a significantly lower IC50 value than the positive control, glucobay, used to treat diabetes mellitus type II. Neither the ethanol nor the aqueous extracts had any effects on tested human malignant cell lines. Our results indicate that the ethanol extract showed the most pronounced in vitro antioxidant and antimicrobial effects, and a potential antidiabetic activity, which can be ascribed to its high flavonoid content. Our results indicate that research of compounds, particularly of flavonoids present in the ethanol extract and their anti-diabetic properties should be examined further.
T2  - Archives of Biological Sciences
T1  - Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts
IS  - 1
VL  - 72
DO  - 10.2298/abs191217004v
SP  - 105
EP  - 115
ER  - 

@article{
author = "Veličković, Ivona and Žižak, Željko and Rajčević, Nemanja and Ivanov, Marija and Soković, Marina and Marin, Petar and Grujić, Slavica",
year = "2020",
abstract = "We investigated the total phenolic and flavonoid contents and the anthocyanin profiles in aqueous, ethanol and acetone extracts of Prunus spinosa (Rosaceae) fruit, and their antioxidant, antibacterial, antifungal, antidiabetic and antitumor properties. The contribution of polyphenol contents to the bioactivity of the extracts was calculated and observed through Pearson’s coefficient of correlation. The acetone extract was the richest in phenols and anthocyanins and the ethanol extract in flavonoids. Cyanidin was the most abundant anthocyanin compound in all examined extracts. The ethanol extract showed the most promising antioxidant activity in DPPH, ABTS and FRAP assays. Tested bacteria were more affected by the ethanol than by the aqueous extract. Both the ethanol and aqueous extracts exhibited potential antidiabetic effects, observed as inhibition of α-amylase and α-glucosidase, enzymes linked with diabetes mellitus type II. The ethanol extract was a potent α-glucosidase-inhibitor with a significantly lower IC50 value than the positive control, glucobay, used to treat diabetes mellitus type II. Neither the ethanol nor the aqueous extracts had any effects on tested human malignant cell lines. Our results indicate that the ethanol extract showed the most pronounced in vitro antioxidant and antimicrobial effects, and a potential antidiabetic activity, which can be ascribed to its high flavonoid content. Our results indicate that research of compounds, particularly of flavonoids present in the ethanol extract and their anti-diabetic properties should be examined further.",
journal = "Archives of Biological Sciences",
title = "Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts",
number = "1",
volume = "72",
doi = "10.2298/abs191217004v",
pages = "105-115"
}

Veličković, I., Žižak, Ž., Rajčević, N., Ivanov, M., Soković, M., Marin, P.,& Grujić, S.. (2020). Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts. in Archives of Biological Sciences, 72(1), 105-115.
https://doi.org/10.2298/abs191217004v

Veličković I, Žižak Ž, Rajčević N, Ivanov M, Soković M, Marin P, Grujić S. Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts. in Archives of Biological Sciences. 2020;72(1):105-115.
doi:10.2298/abs191217004v .

Veličković, Ivona, Žižak, Željko, Rajčević, Nemanja, Ivanov, Marija, Soković, Marina, Marin, Petar, Grujić, Slavica, "Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts" in Archives of Biological Sciences, 72, no. 1 (2020):105-115,
https://doi.org/10.2298/abs191217004v . .