TY  - JOUR
AU  - Čukuranović Kokoris, Jovana
AU  - Ajdžanović, Vladimir
AU  - Pendovski, Lazo
AU  - Ristić, Nataša
AU  - Milošević, Verica
AU  - Dovenska, Monika
AU  - Popovska-Perčinić, Florina
PY  - 2022
UR  - https://www.sciendo.com/article/10.2478/acve-2022-0001
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4965
AB  - Global warming causes an increased ambient temperature and prolonged heatwaves during the summer, which represent stressogenic factors affecting the hypothalamo-pituitary-adrenocortical (HPA) axis in mammals. The aim of this study was to investigate the effects of long-term (7-60 days) exposure to moderately elevated ambient temperature (35 ± 1°C) on the histological aspect and secretory ability of pituitary adrenocorticotropic (ACTH) cells, as well as on the corticosterone output, in adult rats. Stereological parameters of ACTH cells were estimated upon immunohistochemistry. The blood concentrations of ACTH and corticosterone were determined by immunoassays. The volume of ACTH cells in rats exposed to moderately high temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 18.1%, 14.5%, 13.5%, 8.6% and 14.2% respectively, compared to the same parameter in the controls. The volume density of ACTH cells in the groups exposed to elevated temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 40.0%, 33.3%, 26.7%, 13.3% and 26.7% respectively, in comparison with control rats. The plasma concentration of ACTH varied differently (p<0.05) with the duration of exposure to the elevated temperature. The serum concentration of corticosterone was decreased (p<0.05) by 54.9%, 24.4%, 29.9%, 21.1% and 24.4% in groups subjected to moderately high temperature for 7, 14, 21, 30 and 60 days respectively, all compared to the control value. Despite some signs of functional recovery of ACTH cells during the treatment, the impression is that the long-term character of this stressor overcomes the capacity of the HPA axis for resistance.
T2  - Acta Veterinaria
T1  - The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study
IS  - 1
VL  - 72
DO  - 10.2478/acve-2022-0001
SP  - 1
EP  - 15
ER  - 

@article{
author = "Čukuranović Kokoris, Jovana and Ajdžanović, Vladimir and Pendovski, Lazo and Ristić, Nataša and Milošević, Verica and Dovenska, Monika and Popovska-Perčinić, Florina",
year = "2022",
abstract = "Global warming causes an increased ambient temperature and prolonged heatwaves during the summer, which represent stressogenic factors affecting the hypothalamo-pituitary-adrenocortical (HPA) axis in mammals. The aim of this study was to investigate the effects of long-term (7-60 days) exposure to moderately elevated ambient temperature (35 ± 1°C) on the histological aspect and secretory ability of pituitary adrenocorticotropic (ACTH) cells, as well as on the corticosterone output, in adult rats. Stereological parameters of ACTH cells were estimated upon immunohistochemistry. The blood concentrations of ACTH and corticosterone were determined by immunoassays. The volume of ACTH cells in rats exposed to moderately high temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 18.1%, 14.5%, 13.5%, 8.6% and 14.2% respectively, compared to the same parameter in the controls. The volume density of ACTH cells in the groups exposed to elevated temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 40.0%, 33.3%, 26.7%, 13.3% and 26.7% respectively, in comparison with control rats. The plasma concentration of ACTH varied differently (p<0.05) with the duration of exposure to the elevated temperature. The serum concentration of corticosterone was decreased (p<0.05) by 54.9%, 24.4%, 29.9%, 21.1% and 24.4% in groups subjected to moderately high temperature for 7, 14, 21, 30 and 60 days respectively, all compared to the control value. Despite some signs of functional recovery of ACTH cells during the treatment, the impression is that the long-term character of this stressor overcomes the capacity of the HPA axis for resistance.",
journal = "Acta Veterinaria",
title = "The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study",
number = "1",
volume = "72",
doi = "10.2478/acve-2022-0001",
pages = "1-15"
}

Čukuranović Kokoris, J., Ajdžanović, V., Pendovski, L., Ristić, N., Milošević, V., Dovenska, M.,& Popovska-Perčinić, F.. (2022). The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study. in Acta Veterinaria, 72(1), 1-15.
https://doi.org/10.2478/acve-2022-0001

Čukuranović Kokoris J, Ajdžanović V, Pendovski L, Ristić N, Milošević V, Dovenska M, Popovska-Perčinić F. The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study. in Acta Veterinaria. 2022;72(1):1-15.
doi:10.2478/acve-2022-0001 .

Čukuranović Kokoris, Jovana, Ajdžanović, Vladimir, Pendovski, Lazo, Ristić, Nataša, Milošević, Verica, Dovenska, Monika, Popovska-Perčinić, Florina, "The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study" in Acta Veterinaria, 72, no. 1 (2022):1-15,
https://doi.org/10.2478/acve-2022-0001 . .

TY  - JOUR
AU  - Milošević, Verica
AU  - Severs, Walter B
AU  - Ristić, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Popovska-Perčinić, Florina V
AU  - Šošić-Jurjević, Branka 
AU  - Pendovski, Lazo B
AU  - Trifunović, Svetlana
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
PY  - 2018
UR  - http://www.hh.um.es/Abstracts/Vol_/_/__11984.htm
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3103
AB  - Genistein (G) and related soy phytoestrogens have been studied for potential usefulness in different chronic diseases, and may ameliorate signs of aging. They have a profound influence on the hypothalamo-pituitary-adrenal (HPA) axis. The present study utilized the rat model of mild andropause to thoroughly evaluate the effects of G and soy extract on the adrenal gland and related blood hormones. Adult male rats were orchidectomized (Orx) or sham operated (SO). Orx rats received daily subcutaneous injections for 3 weeks of solvent, or G (Orx+G, 30 mg/kg), or commercial soy extract (Orx+Soy, 30 mg/kg). Adrenal glands and blood were harvested at the end of the treatment for hormone analyses, histology and design-based stereology. Compared to SO rats Orx evoked significant (P<0.05) changes including: the replicating cell number in the 3 adrenocortical zones; vascularity and cortical volume and blood levels of adrenocorticotropic hormone (ACTH), aldosterone and dehydroepiandrosterone (DHEA). When comparing Orx vs. Orx+G groups the following significant (P<0.05) changes were observed: a further increase in number of replicating cells in zonas glomerulosa and reticularis, vasculature network presence, cortical and zona reticularis volumes, ACTH and corticosterone concentrations, and lower DHEA levels. Comparing Orx vs. Orx+Soy resulted in elevated (P<0.05) ACTH and corticosterone levels. Structural integrity of the adrenal gland was unchanged vs. SO rats. Overall, G and soy extract treatments resulted in proliferative activity and/or vasculature support in the adrenal cortex. The data and current literature support the impression of a beneficial effect of soy components on the homeostatic response to stress.
T2  - Histology and Histopathology
T1  - Soy isoflavone effects on the adrenal glands of orchidectomized adult male rats: a comprehensive histological and hormonal study.
IS  - 8
VL  - 33
DO  - 10.14670/HH-11-984
SP  - 843
EP  - 857
ER  - 

@article{
author = "Milošević, Verica and Severs, Walter B and Ristić, Nataša and Manojlović-Stojanoski, Milica and Popovska-Perčinić, Florina V and Šošić-Jurjević, Branka  and Pendovski, Lazo B and Trifunović, Svetlana and Miler, Marko and Ajdžanović, Vladimir",
year = "2018",
abstract = "Genistein (G) and related soy phytoestrogens have been studied for potential usefulness in different chronic diseases, and may ameliorate signs of aging. They have a profound influence on the hypothalamo-pituitary-adrenal (HPA) axis. The present study utilized the rat model of mild andropause to thoroughly evaluate the effects of G and soy extract on the adrenal gland and related blood hormones. Adult male rats were orchidectomized (Orx) or sham operated (SO). Orx rats received daily subcutaneous injections for 3 weeks of solvent, or G (Orx+G, 30 mg/kg), or commercial soy extract (Orx+Soy, 30 mg/kg). Adrenal glands and blood were harvested at the end of the treatment for hormone analyses, histology and design-based stereology. Compared to SO rats Orx evoked significant (P<0.05) changes including: the replicating cell number in the 3 adrenocortical zones; vascularity and cortical volume and blood levels of adrenocorticotropic hormone (ACTH), aldosterone and dehydroepiandrosterone (DHEA). When comparing Orx vs. Orx+G groups the following significant (P<0.05) changes were observed: a further increase in number of replicating cells in zonas glomerulosa and reticularis, vasculature network presence, cortical and zona reticularis volumes, ACTH and corticosterone concentrations, and lower DHEA levels. Comparing Orx vs. Orx+Soy resulted in elevated (P<0.05) ACTH and corticosterone levels. Structural integrity of the adrenal gland was unchanged vs. SO rats. Overall, G and soy extract treatments resulted in proliferative activity and/or vasculature support in the adrenal cortex. The data and current literature support the impression of a beneficial effect of soy components on the homeostatic response to stress.",
journal = "Histology and Histopathology",
title = "Soy isoflavone effects on the adrenal glands of orchidectomized adult male rats: a comprehensive histological and hormonal study.",
number = "8",
volume = "33",
doi = "10.14670/HH-11-984",
pages = "843-857"
}

Milošević, V., Severs, W. B., Ristić, N., Manojlović-Stojanoski, M., Popovska-Perčinić, F. V., Šošić-Jurjević, B., Pendovski, L. B., Trifunović, S., Miler, M.,& Ajdžanović, V.. (2018). Soy isoflavone effects on the adrenal glands of orchidectomized adult male rats: a comprehensive histological and hormonal study.. in Histology and Histopathology, 33(8), 843-857.
https://doi.org/10.14670/HH-11-984

Milošević V, Severs WB, Ristić N, Manojlović-Stojanoski M, Popovska-Perčinić FV, Šošić-Jurjević B, Pendovski LB, Trifunović S, Miler M, Ajdžanović V. Soy isoflavone effects on the adrenal glands of orchidectomized adult male rats: a comprehensive histological and hormonal study.. in Histology and Histopathology. 2018;33(8):843-857.
doi:10.14670/HH-11-984 .

Milošević, Verica, Severs, Walter B, Ristić, Nataša, Manojlović-Stojanoski, Milica, Popovska-Perčinić, Florina V, Šošić-Jurjević, Branka , Pendovski, Lazo B, Trifunović, Svetlana, Miler, Marko, Ajdžanović, Vladimir, "Soy isoflavone effects on the adrenal glands of orchidectomized adult male rats: a comprehensive histological and hormonal study." in Histology and Histopathology, 33, no. 8 (2018):843-857,
https://doi.org/10.14670/HH-11-984 . .