TY  - JOUR
AU  - Popovska-Perčinić, Florina
AU  - Manojlović-Stojanoski, Milica
AU  - Pendovski, Lazo
AU  - Dinevska Kjovkarovska, Suzana
AU  - Miova, Biljana
AU  - Grubin, Jasmina
AU  - Milošević, Verica
AU  - Ajdžanović, Vladimir
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/123456789/3867
AB  - Objective: As a consequence of global warming, the increase in the average annual temperature is observed, while the living organisms actively adapt to these changes. High environmental temperature initiates numerous physiological, autonomic, and behavioral responses, and activates the stress response. Thus, the aim of the study was to investigate effect of a moderate increase in ambient temperature on the activity of the hypothalamic-pituitary-adrenocortical (HPA) axis by determining histological changes in adrenal glands and hormonal levels in adult male rats.

Materials and methods: In this experimental study, the morpho-functional state of adrenal glands was estimated by stereological evaluation of parameters, including the adrenal volume, adrenocortical cell/nuclear size and number, and the volume density of vascular tissues after four days of exposure to a moderate increase in ambient temperature of 35 ± 1˚C. Novelli histochemical and vascular endothelial growth factor (VEGF) immunohistochemical staining provided insight into the adrenal gland vascular network. Additionally, the adrenal levels of aldosterone, corticosterone, and pituitary adrenocorticotropic hormone (ACTH) were determined as crucial indicators of the hypothalamic-pituitaryadrenocortical (HPA) axis activity.

Results: Prolonged exposure to a moderate increase in ambient temperature for four days resulted in a significant increase in ACTH level up to 24%, which altered adrenal glands both structurally and functionally. The adrenocortical volume and number of cells in all cortical zones were markedly increased (P<0.05). A statistically significant increase was shown in the level of aldosterone (16%) and corticosterone (25%) in serum levels of individuals.

Conclusion: Increased activity of the HPA axis reflects the response to a moderate increase in ambient temperature during four days, showing the capacity of the HPA axis to adapt the organism to daily temperature changes.
PB  - Royan Institute
T2  - Cell Journal
T1  - A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats
IS  - 4
VL  - 22
DO  - 10.22074/cellj.2021.6827
SP  - 415
EP  - 424
ER  - 

@article{
author = "Popovska-Perčinić, Florina and Manojlović-Stojanoski, Milica and Pendovski, Lazo and Dinevska Kjovkarovska, Suzana and Miova, Biljana and Grubin, Jasmina and Milošević, Verica and Ajdžanović, Vladimir",
year = "2021",
abstract = "Objective: As a consequence of global warming, the increase in the average annual temperature is observed, while the living organisms actively adapt to these changes. High environmental temperature initiates numerous physiological, autonomic, and behavioral responses, and activates the stress response. Thus, the aim of the study was to investigate effect of a moderate increase in ambient temperature on the activity of the hypothalamic-pituitary-adrenocortical (HPA) axis by determining histological changes in adrenal glands and hormonal levels in adult male rats.

Materials and methods: In this experimental study, the morpho-functional state of adrenal glands was estimated by stereological evaluation of parameters, including the adrenal volume, adrenocortical cell/nuclear size and number, and the volume density of vascular tissues after four days of exposure to a moderate increase in ambient temperature of 35 ± 1˚C. Novelli histochemical and vascular endothelial growth factor (VEGF) immunohistochemical staining provided insight into the adrenal gland vascular network. Additionally, the adrenal levels of aldosterone, corticosterone, and pituitary adrenocorticotropic hormone (ACTH) were determined as crucial indicators of the hypothalamic-pituitaryadrenocortical (HPA) axis activity.

Results: Prolonged exposure to a moderate increase in ambient temperature for four days resulted in a significant increase in ACTH level up to 24%, which altered adrenal glands both structurally and functionally. The adrenocortical volume and number of cells in all cortical zones were markedly increased (P<0.05). A statistically significant increase was shown in the level of aldosterone (16%) and corticosterone (25%) in serum levels of individuals.

Conclusion: Increased activity of the HPA axis reflects the response to a moderate increase in ambient temperature during four days, showing the capacity of the HPA axis to adapt the organism to daily temperature changes.",
publisher = "Royan Institute",
journal = "Cell Journal",
title = "A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats",
number = "4",
volume = "22",
doi = "10.22074/cellj.2021.6827",
pages = "415-424"
}

Popovska-Perčinić, F., Manojlović-Stojanoski, M., Pendovski, L., Dinevska Kjovkarovska, S., Miova, B., Grubin, J., Milošević, V.,& Ajdžanović, V.. (2021). A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats. in Cell Journal
Royan Institute., 22(4), 415-424.
https://doi.org/10.22074/cellj.2021.6827

Popovska-Perčinić F, Manojlović-Stojanoski M, Pendovski L, Dinevska Kjovkarovska S, Miova B, Grubin J, Milošević V, Ajdžanović V. A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats. in Cell Journal. 2021;22(4):415-424.
doi:10.22074/cellj.2021.6827 .

Popovska-Perčinić, Florina, Manojlović-Stojanoski, Milica, Pendovski, Lazo, Dinevska Kjovkarovska, Suzana, Miova, Biljana, Grubin, Jasmina, Milošević, Verica, Ajdžanović, Vladimir, "A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats" in Cell Journal, 22, no. 4 (2021):415-424,
https://doi.org/10.22074/cellj.2021.6827 . .

TY  - CONF
AU  - Popovska-Perčinić, Florina
AU  - Ajdžanović, Vladimir
AU  - Dinevska-Kjovkarovska, Suzana
AU  - Ristić, Nataša
AU  - Miova, Biljana
AU  - Filipović, Branko
AU  - Milošević, Verica
PY  - 2014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6251
AB  - Pituitary ACTH producing cells are very sensitive towards changes in duration of the heat exposure. The aim of this study was to examine the effects of 4-day continuous exposure of rats to moderate heat on histological and secretory characteristics of ACTH cells in adult male rats. The experimental group was exposed to 35±1°C for 4 days, whereas the control group was kept at room temperature during the same period. ACTH cells were studied using the immunohistochemical and immunofluorescent procedures. The morphometry was conducted using M42 multipurpose test system, while the hormone concentrations were determined by ACTH IMMULITE assay and corticosterone ELISA test. Compared to the controls, a significant (p<0.05) decrease of the body weight by 19.8% and significant (p<0.05) increase of the absolute and relative volumes of the pituitary gland by 23.1% and 36.1% respectively, were registered. It was found that in experimental group neither the localization nor the shape of ACTH cells was significantly changed, but the intensity of immunofluorescence signal was significantly (p<0.05) decreased. The cellular and nuclear volumes of ACTH cells were increased (p<0.05) by 6.9% and 14.3% respectively, while the volume density of ACTH cells was decreased (p<0.05) by 20.0%, in comparison with the corresponding controls. The observed changes were in accordance with increased (p<0.05) values of blood ACTH and corticosterone concentrations by 23.7% and 11.8%, respectively. Based upon the histological and hormonal data, it can be concluded that 4-day continuous exposure to moderate heat causes stimulation of the pituitary ACTH cells in rats.
PB  - Belgrade: Serbian Physiology Society
C3  - Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia
T1  - The stimulatory effect of moderate heat on rat pituitary ACTH cells: histological and hormonal study
SP  - 165
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6251
ER  - 

@conference{
author = "Popovska-Perčinić, Florina and Ajdžanović, Vladimir and Dinevska-Kjovkarovska, Suzana and Ristić, Nataša and Miova, Biljana and Filipović, Branko and Milošević, Verica",
year = "2014",
abstract = "Pituitary ACTH producing cells are very sensitive towards changes in duration of the heat exposure. The aim of this study was to examine the effects of 4-day continuous exposure of rats to moderate heat on histological and secretory characteristics of ACTH cells in adult male rats. The experimental group was exposed to 35±1°C for 4 days, whereas the control group was kept at room temperature during the same period. ACTH cells were studied using the immunohistochemical and immunofluorescent procedures. The morphometry was conducted using M42 multipurpose test system, while the hormone concentrations were determined by ACTH IMMULITE assay and corticosterone ELISA test. Compared to the controls, a significant (p<0.05) decrease of the body weight by 19.8% and significant (p<0.05) increase of the absolute and relative volumes of the pituitary gland by 23.1% and 36.1% respectively, were registered. It was found that in experimental group neither the localization nor the shape of ACTH cells was significantly changed, but the intensity of immunofluorescence signal was significantly (p<0.05) decreased. The cellular and nuclear volumes of ACTH cells were increased (p<0.05) by 6.9% and 14.3% respectively, while the volume density of ACTH cells was decreased (p<0.05) by 20.0%, in comparison with the corresponding controls. The observed changes were in accordance with increased (p<0.05) values of blood ACTH and corticosterone concentrations by 23.7% and 11.8%, respectively. Based upon the histological and hormonal data, it can be concluded that 4-day continuous exposure to moderate heat causes stimulation of the pituitary ACTH cells in rats.",
publisher = "Belgrade: Serbian Physiology Society",
journal = "Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia",
title = "The stimulatory effect of moderate heat on rat pituitary ACTH cells: histological and hormonal study",
pages = "165",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6251"
}

Popovska-Perčinić, F., Ajdžanović, V., Dinevska-Kjovkarovska, S., Ristić, N., Miova, B., Filipović, B.,& Milošević, V.. (2014). The stimulatory effect of moderate heat on rat pituitary ACTH cells: histological and hormonal study. in Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia
Belgrade: Serbian Physiology Society., 165.
https://hdl.handle.net/21.15107/rcub_ibiss_6251

Popovska-Perčinić F, Ajdžanović V, Dinevska-Kjovkarovska S, Ristić N, Miova B, Filipović B, Milošević V. The stimulatory effect of moderate heat on rat pituitary ACTH cells: histological and hormonal study. in Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia. 2014;:165.
https://hdl.handle.net/21.15107/rcub_ibiss_6251 .

Popovska-Perčinić, Florina, Ajdžanović, Vladimir, Dinevska-Kjovkarovska, Suzana, Ristić, Nataša, Miova, Biljana, Filipović, Branko, Milošević, Verica, "The stimulatory effect of moderate heat on rat pituitary ACTH cells: histological and hormonal study" in Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia (2014):165,
https://hdl.handle.net/21.15107/rcub_ibiss_6251 .