TY  - JOUR
AU  - Harhaji-Trajković, Ljubica
AU  - Isaković, Aleksandra
AU  - Zogović, Nevena
AU  - Marković, Zoran
AU  - Todorović-Marković, Biljana
AU  - Nikolić, Nadežda
AU  - Vranješ-Đurić, Sanja
AU  - Marković, Ivanka
AU  - Trajković, Vladimir
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6544
AB  - Using the rat glioma cell line C6 and the human glioma cell line U251, we demonstrate the multiple mechanisms underlying the in vitro anticancer effects of the C(60) fullerene water suspension (nano-C(60) or nC(60)) produced by solvent exchange method. Nano-C(60) in a dose-dependent manner reduced the tumor cell numbers after 24 h of incubation. The observed antiglioma action of nC(60) at high concentration (1 microg/ml) was due to a reactive oxygen species-mediated necrotic cell damage that was partly dependent on oxidative stress-induced activation of extracellular signal-regulated kinase (ERK). On the other hand, low-dose nC(60) (0.25 microg/ml) did not induce either necrotic or apoptotic cell death, but caused oxidative stress/ERK-independent cell cycle block in G(2)/M phase and subsequent inhibition of tumor cell proliferation. Treatment with either high-dose or low-dose nC(60) caused the appearance of acidified intracytoplasmic vesicles indicative of autophagy, but only the antiglioma effect of low-dose nC(60) was significantly attenuated by inhibiting autophagy with bafilomycin A1. Importantly, primary rat astrocytes were less sensitive than their transformed counterparts to a cytostatic action of low-dose nC(60). These data provide grounds for further development of nC(60) as an anticancer agent.
PB  - Elsevier BV
T2  - European Journal of Pharmacology
T1  - Multiple mechanisms underlying the anticancer action of nanocrystalline fullerene
IS  - 1-3
VL  - 568
DO  - 10.1016/j.ejphar.2007.04.041
SP  - 89
EP  - 98
ER  - 

@article{
author = "Harhaji-Trajković, Ljubica and Isaković, Aleksandra and Zogović, Nevena and Marković, Zoran and Todorović-Marković, Biljana and Nikolić, Nadežda and Vranješ-Đurić, Sanja and Marković, Ivanka and Trajković, Vladimir",
year = "2007",
abstract = "Using the rat glioma cell line C6 and the human glioma cell line U251, we demonstrate the multiple mechanisms underlying the in vitro anticancer effects of the C(60) fullerene water suspension (nano-C(60) or nC(60)) produced by solvent exchange method. Nano-C(60) in a dose-dependent manner reduced the tumor cell numbers after 24 h of incubation. The observed antiglioma action of nC(60) at high concentration (1 microg/ml) was due to a reactive oxygen species-mediated necrotic cell damage that was partly dependent on oxidative stress-induced activation of extracellular signal-regulated kinase (ERK). On the other hand, low-dose nC(60) (0.25 microg/ml) did not induce either necrotic or apoptotic cell death, but caused oxidative stress/ERK-independent cell cycle block in G(2)/M phase and subsequent inhibition of tumor cell proliferation. Treatment with either high-dose or low-dose nC(60) caused the appearance of acidified intracytoplasmic vesicles indicative of autophagy, but only the antiglioma effect of low-dose nC(60) was significantly attenuated by inhibiting autophagy with bafilomycin A1. Importantly, primary rat astrocytes were less sensitive than their transformed counterparts to a cytostatic action of low-dose nC(60). These data provide grounds for further development of nC(60) as an anticancer agent.",
publisher = "Elsevier BV",
journal = "European Journal of Pharmacology",
title = "Multiple mechanisms underlying the anticancer action of nanocrystalline fullerene",
number = "1-3",
volume = "568",
doi = "10.1016/j.ejphar.2007.04.041",
pages = "89-98"
}

Harhaji-Trajković, L., Isaković, A., Zogović, N., Marković, Z., Todorović-Marković, B., Nikolić, N., Vranješ-Đurić, S., Marković, I.,& Trajković, V.. (2007). Multiple mechanisms underlying the anticancer action of nanocrystalline fullerene. in European Journal of Pharmacology
Elsevier BV., 568(1-3), 89-98.
https://doi.org/10.1016/j.ejphar.2007.04.041

Harhaji-Trajković L, Isaković A, Zogović N, Marković Z, Todorović-Marković B, Nikolić N, Vranješ-Đurić S, Marković I, Trajković V. Multiple mechanisms underlying the anticancer action of nanocrystalline fullerene. in European Journal of Pharmacology. 2007;568(1-3):89-98.
doi:10.1016/j.ejphar.2007.04.041 .

Harhaji-Trajković, Ljubica, Isaković, Aleksandra, Zogović, Nevena, Marković, Zoran, Todorović-Marković, Biljana, Nikolić, Nadežda, Vranješ-Đurić, Sanja, Marković, Ivanka, Trajković, Vladimir, "Multiple mechanisms underlying the anticancer action of nanocrystalline fullerene" in European Journal of Pharmacology, 568, no. 1-3 (2007):89-98,
https://doi.org/10.1016/j.ejphar.2007.04.041 . .