TY  - CONF
AU  - Jarić, Ivana
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Pendoski, Laze
AU  - Žikić, Dragan
AU  - Ušćebrka, Gordana
AU  - Milošević, Verica
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6037
AB  - Diosgenin is bioactive, steroidal saponin from Dioscorea viliosa (Wild Yam) and appears to be novel promising substance for the treatment of menopausal symptoms. In addition, diosgenin may manipulate the endogenous steroid hormone levels and may affect the numerous oxidative stress pathways/molecules. Since decline in the steroid hormone levels as well as increase in oxidative stress in the ovaries play an integral part of the menopause, this study aimed to investigate the effects of diosgenin on production of estradiol and progesterone, as well as to investigated the effects of diosgenin on the expression of genes encoding the antioxidant enzymes (Cu, Zn-superoxide dismutase (CuZnSOD), Mn-superoxide dismutase (MnSOD), glutathione peroxidase (GPX), and catalase (CAD) in ovary of acyclic-middle aged female rats. Middle-aged (l3-months-old) acyclic female Wistar rats in constant diestrus stage, which represent tractable model to human menopause, were divided into control and diosgenin treated groups.Diosgenin (100 mgfl<g b.w.lday) was orally applied for 4 weeks, while C group received the vehicle alone. In our experimental model, diosgenin administration to acyclic middle-aged rats significantly increased estradiol and progesterone serum concentrations when compared to the corresponding control. Gene expression analysis revealed that diosgenin significantly down-regulate all examined enzymes involved in antioxidative defense. The down-regulation were especially pronounced in CuZnSOD and CAT, indicating that the diosgenin may decrease the pool of reactive oxygen species in the ovary directly due and may act as free radical scavengers. Therefore, our results indicate beneficial potential of diosgenin in the middle-aged rat ovary.
PB  - Skopje: Faculty of Veterinary Medicine
C3  - Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia
T1  - Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats
SP  - 147
EP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6037
ER  - 

@conference{
author = "Jarić, Ivana and Miler, Marko and Ajdžanović, Vladimir and Živanović, Jasmina and Pendoski, Laze and Žikić, Dragan and Ušćebrka, Gordana and Milošević, Verica",
year = "2018",
abstract = "Diosgenin is bioactive, steroidal saponin from Dioscorea viliosa (Wild Yam) and appears to be novel promising substance for the treatment of menopausal symptoms. In addition, diosgenin may manipulate the endogenous steroid hormone levels and may affect the numerous oxidative stress pathways/molecules. Since decline in the steroid hormone levels as well as increase in oxidative stress in the ovaries play an integral part of the menopause, this study aimed to investigate the effects of diosgenin on production of estradiol and progesterone, as well as to investigated the effects of diosgenin on the expression of genes encoding the antioxidant enzymes (Cu, Zn-superoxide dismutase (CuZnSOD), Mn-superoxide dismutase (MnSOD), glutathione peroxidase (GPX), and catalase (CAD) in ovary of acyclic-middle aged female rats. Middle-aged (l3-months-old) acyclic female Wistar rats in constant diestrus stage, which represent tractable model to human menopause, were divided into control and diosgenin treated groups.Diosgenin (100 mgfl<g b.w.lday) was orally applied for 4 weeks, while C group received the vehicle alone. In our experimental model, diosgenin administration to acyclic middle-aged rats significantly increased estradiol and progesterone serum concentrations when compared to the corresponding control. Gene expression analysis revealed that diosgenin significantly down-regulate all examined enzymes involved in antioxidative defense. The down-regulation were especially pronounced in CuZnSOD and CAT, indicating that the diosgenin may decrease the pool of reactive oxygen species in the ovary directly due and may act as free radical scavengers. Therefore, our results indicate beneficial potential of diosgenin in the middle-aged rat ovary.",
publisher = "Skopje: Faculty of Veterinary Medicine",
journal = "Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia",
title = "Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats",
pages = "147-147",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6037"
}

Jarić, I., Miler, M., Ajdžanović, V., Živanović, J., Pendoski, L., Žikić, D., Ušćebrka, G.,& Milošević, V.. (2018). Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats. in Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia
Skopje: Faculty of Veterinary Medicine., 147-147.
https://hdl.handle.net/21.15107/rcub_ibiss_6037

Jarić I, Miler M, Ajdžanović V, Živanović J, Pendoski L, Žikić D, Ušćebrka G, Milošević V. Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats. in Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia. 2018;:147-147.
https://hdl.handle.net/21.15107/rcub_ibiss_6037 .

Jarić, Ivana, Miler, Marko, Ajdžanović, Vladimir, Živanović, Jasmina, Pendoski, Laze, Žikić, Dragan, Ušćebrka, Gordana, Milošević, Verica, "Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats" in Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia (2018):147-147,
https://hdl.handle.net/21.15107/rcub_ibiss_6037 .

TY  - JOUR
AU  - Žikić, Dragan
AU  - Stojanović, Slobodan
AU  - Đukić-Stojčić, Mirjana
AU  - Kanački, Zdenko
AU  - Milošević, Verica
AU  - Ušćebrka, Gordana
PY  - 2016
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=1450-91561601027Z
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2602
AB  - Morphological characteristics of skeletal muscles of slow- and medium-growing strains of chickens are very important for meat quality and comparison with fast-growing strains. The aim of this paper was to evaluate morphological parameters of breast and thigh muscles of slow- and mediumgrowing strains in a free-range system. The slow-growing strains used in the experiment were autochthonous breeds Sombor crested and Banat naked neck, and the medium-growing strain was Red-bro. The tissue samples were taken from the thigh muscle and muscles of the breast of 10 chickens of each breed. Samples were stained with hematoxylin - eosin and enzyme succinate - dehydrogenase (SDH). The following morphological parameters were observed: diameter of muscle cells, nucleo-cytoplasmic ratio of muscle cells, volume density of connective tissue within the muscle and the presence of red, white and intermediate muscle cell types. Between strains, the type of muscle or genotype didn’t have significant effects on diameters of muscle cells and nucleo-cytoplasmic ratio in the muscle cells. Results indicated that genotype had significant effect on volume density of the connective tissue in breast muscles. Red muscle cells were, in all strains, significantly more represented in m. biceps femoris than m. pectoralis superficialis. Genotype had significant effect on ratio between connective tissue and muscle cells and no significant effects on other morphological parameters. [Projekat Ministarstva nauke Republike Srbije, br. TR 31033]
T2  - Biotechnology in Animal Husbandry
T1  - Morphological characteristics of breast and thigh muscles of slow- and medium growing strains of chickens
IS  - 1
VL  - 32
DO  - 10.2298/BAH1601027Z
SP  - 27
EP  - 35
ER  - 

@article{
author = "Žikić, Dragan and Stojanović, Slobodan and Đukić-Stojčić, Mirjana and Kanački, Zdenko and Milošević, Verica and Ušćebrka, Gordana",
year = "2016",
abstract = "Morphological characteristics of skeletal muscles of slow- and medium-growing strains of chickens are very important for meat quality and comparison with fast-growing strains. The aim of this paper was to evaluate morphological parameters of breast and thigh muscles of slow- and mediumgrowing strains in a free-range system. The slow-growing strains used in the experiment were autochthonous breeds Sombor crested and Banat naked neck, and the medium-growing strain was Red-bro. The tissue samples were taken from the thigh muscle and muscles of the breast of 10 chickens of each breed. Samples were stained with hematoxylin - eosin and enzyme succinate - dehydrogenase (SDH). The following morphological parameters were observed: diameter of muscle cells, nucleo-cytoplasmic ratio of muscle cells, volume density of connective tissue within the muscle and the presence of red, white and intermediate muscle cell types. Between strains, the type of muscle or genotype didn’t have significant effects on diameters of muscle cells and nucleo-cytoplasmic ratio in the muscle cells. Results indicated that genotype had significant effect on volume density of the connective tissue in breast muscles. Red muscle cells were, in all strains, significantly more represented in m. biceps femoris than m. pectoralis superficialis. Genotype had significant effect on ratio between connective tissue and muscle cells and no significant effects on other morphological parameters. [Projekat Ministarstva nauke Republike Srbije, br. TR 31033]",
journal = "Biotechnology in Animal Husbandry",
title = "Morphological characteristics of breast and thigh muscles of slow- and medium growing strains of chickens",
number = "1",
volume = "32",
doi = "10.2298/BAH1601027Z",
pages = "27-35"
}

Žikić, D., Stojanović, S., Đukić-Stojčić, M., Kanački, Z., Milošević, V.,& Ušćebrka, G.. (2016). Morphological characteristics of breast and thigh muscles of slow- and medium growing strains of chickens. in Biotechnology in Animal Husbandry, 32(1), 27-35.
https://doi.org/10.2298/BAH1601027Z

Žikić D, Stojanović S, Đukić-Stojčić M, Kanački Z, Milošević V, Ušćebrka G. Morphological characteristics of breast and thigh muscles of slow- and medium growing strains of chickens. in Biotechnology in Animal Husbandry. 2016;32(1):27-35.
doi:10.2298/BAH1601027Z .

Žikić, Dragan, Stojanović, Slobodan, Đukić-Stojčić, Mirjana, Kanački, Zdenko, Milošević, Verica, Ušćebrka, Gordana, "Morphological characteristics of breast and thigh muscles of slow- and medium growing strains of chickens" in Biotechnology in Animal Husbandry, 32, no. 1 (2016):27-35,
https://doi.org/10.2298/BAH1601027Z . .

TY  - JOUR
AU  - Jarić, Ivana
AU  - Ristić, Nataša
AU  - Trifunović, Svetlana
AU  - Manojlović-Stojanoski, Milica
AU  - Milošević, Verica
AU  - Žikić, Dragan
AU  - Nestorović, Nataša
PY  - 2012
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6026
AB  - The effects of short-term genistein exposure on ovarian folliculogenesis in immature rats were examined stereologically. To determine whether genistein acts as an estrogen agonist or antagonist, the results were compared with the effects of 17a-ethynylestradiol. Immature
female rats received 50 mg/kg/bw of genistein in dimethyl sulfoxide subcutaneously daily for three
consecutive days from 18 to 20 days. The second group was injected with 1 lg/kg/bw of 17a-ethynylestradiol in olive oil in the same schedule. Each group had a corresponding control. Genistein
increased ovary and ovarian stroma volumes by 18.50% (P < 0.05) and 53.40% (P < 0.05), respectively, and changed the parenchyma to stroma ratio in favor of stroma. Genistein induced
decreases in the number of primordial (by 17.23%; P < 0.05), primary (16.62%; P < 0.05), and secondary follicles (12.29%: P < 0.05), whereas the number of atretic secondary follicles increased
(5.10-fold; P < 0.05). The number of healthy large follicles was raised by 27.3% (P < 0.05), accompanied by 35.64% more atretic large follicles (P < 0.05). Similarly to genistein, estradiol changed the
parenchyma to stroma ratio in favor of stroma, and reduced the number of primordial follicles, but
the number of primary follicles was elevated. There were more healthy and atretic small and large
follicles. In conclusion, genistein acted as an estrogen antagonist and had an inhibitory effect on
the initial phase of folliculogenesis. In the other phases, genistein acted as an estrogen agonist,
stimulating transition from the preantral to antral stage of folliculogenesis, and altering the ratio
of follicular parenchyma and ovarian stroma in favor of stroma.
PB  - Hoboken: Wiley
T2  - Microscopy Research and Technique
T1  - Genistein affects ovarian folliculogenesis: A stereological study
IS  - 12
VL  - 75
DO  - 10.1002/jemt.22117
SP  - 1691
EP  - 1699
ER  - 

@article{
author = "Jarić, Ivana and Ristić, Nataša and Trifunović, Svetlana and Manojlović-Stojanoski, Milica and Milošević, Verica and Žikić, Dragan and Nestorović, Nataša",
year = "2012",
abstract = "The effects of short-term genistein exposure on ovarian folliculogenesis in immature rats were examined stereologically. To determine whether genistein acts as an estrogen agonist or antagonist, the results were compared with the effects of 17a-ethynylestradiol. Immature
female rats received 50 mg/kg/bw of genistein in dimethyl sulfoxide subcutaneously daily for three
consecutive days from 18 to 20 days. The second group was injected with 1 lg/kg/bw of 17a-ethynylestradiol in olive oil in the same schedule. Each group had a corresponding control. Genistein
increased ovary and ovarian stroma volumes by 18.50% (P < 0.05) and 53.40% (P < 0.05), respectively, and changed the parenchyma to stroma ratio in favor of stroma. Genistein induced
decreases in the number of primordial (by 17.23%; P < 0.05), primary (16.62%; P < 0.05), and secondary follicles (12.29%: P < 0.05), whereas the number of atretic secondary follicles increased
(5.10-fold; P < 0.05). The number of healthy large follicles was raised by 27.3% (P < 0.05), accompanied by 35.64% more atretic large follicles (P < 0.05). Similarly to genistein, estradiol changed the
parenchyma to stroma ratio in favor of stroma, and reduced the number of primordial follicles, but
the number of primary follicles was elevated. There were more healthy and atretic small and large
follicles. In conclusion, genistein acted as an estrogen antagonist and had an inhibitory effect on
the initial phase of folliculogenesis. In the other phases, genistein acted as an estrogen agonist,
stimulating transition from the preantral to antral stage of folliculogenesis, and altering the ratio
of follicular parenchyma and ovarian stroma in favor of stroma.",
publisher = "Hoboken: Wiley",
journal = "Microscopy Research and Technique",
title = "Genistein affects ovarian folliculogenesis: A stereological study",
number = "12",
volume = "75",
doi = "10.1002/jemt.22117",
pages = "1691-1699"
}

Jarić, I., Ristić, N., Trifunović, S., Manojlović-Stojanoski, M., Milošević, V., Žikić, D.,& Nestorović, N.. (2012). Genistein affects ovarian folliculogenesis: A stereological study. in Microscopy Research and Technique
Hoboken: Wiley., 75(12), 1691-1699.
https://doi.org/10.1002/jemt.22117

Jarić I, Ristić N, Trifunović S, Manojlović-Stojanoski M, Milošević V, Žikić D, Nestorović N. Genistein affects ovarian folliculogenesis: A stereological study. in Microscopy Research and Technique. 2012;75(12):1691-1699.
doi:10.1002/jemt.22117 .

Jarić, Ivana, Ristić, Nataša, Trifunović, Svetlana, Manojlović-Stojanoski, Milica, Milošević, Verica, Žikić, Dragan, Nestorović, Nataša, "Genistein affects ovarian folliculogenesis: A stereological study" in Microscopy Research and Technique, 75, no. 12 (2012):1691-1699,
https://doi.org/10.1002/jemt.22117 . .