TY  - CONF
AU  - Živanović, Jasmina
AU  - Kouroussis, Emilia
AU  - Kohl, Joshua
AU  - Miler, Marko
AU  - Mitchell, Sarah
AU  - Mitchell, James
AU  - Schwartz, Guenter
AU  - Milošević, Verica
AU  - Filipović R., Miloš
PY  - 2019
UR  - https://www.bcp.fu-berlin.de/en/biologie/arbeitsgruppen/mikrobiologie/ag_antelmann/meetings/190915_redox_res/Flyer-Sant-Feliu-2019-final6.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6039
AB  - For couple of decades aging is believed to be a consequence of damages accumulated by increased production of reactive oxygen species (ROS). Opposite to that, dietary restriction attempts to extend life have been linked to increased H2S production. We hypothesized that reacting with sulfenic acids (formed by ROS) H2S would rescue cysteine residue by forming persulfides. Persulfides are better ROS scavengers and could form protein S-sulfonates that could be cleaved back by disulfide reductase to restore free thiolate. This rescue loop would thus prevent irreversible thiol hyperoxidation. Using dimedone switch method we could first show that persulfidation is a posttranslational modification that is evolutionarily conserved (bacteria, flies, worms, mice and humans). Then we could also observe that thioredoxin readily catalyzes the reduction of cysteine S-sulfonate, two orders of magnitude faster than the reduction of corresponding disulfide. The persulfidation levels/H2S producing ability positively correlated with the ability of yeast, worms and mammalian cells to resist stress caused by ROS. Persulfidation declines with aging in worms, rats and mice, due to the downregulation of H2S producing enzymes, but dietary restriction in mice and worms restores persulfide levels and improves their lifespan. Based on these data we suggest that persulfidation represents an evolutionarily conserved anti-aging mechanism.
PB  - Berlin: Freie Universität Berlin
C3  - SPP1710 Conference: Thiol-based switches and redox regulation - from microbes to men; 2019 Sep 15-20; Sant Feliu de Guixols, Spain
T1  - Protein Persulfidation Protects Against Aging
SP  - 169
EP  - 169
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6039
ER  - 

@conference{
author = "Živanović, Jasmina and Kouroussis, Emilia and Kohl, Joshua and Miler, Marko and Mitchell, Sarah and Mitchell, James and Schwartz, Guenter and Milošević, Verica and Filipović R., Miloš",
year = "2019",
abstract = "For couple of decades aging is believed to be a consequence of damages accumulated by increased production of reactive oxygen species (ROS). Opposite to that, dietary restriction attempts to extend life have been linked to increased H2S production. We hypothesized that reacting with sulfenic acids (formed by ROS) H2S would rescue cysteine residue by forming persulfides. Persulfides are better ROS scavengers and could form protein S-sulfonates that could be cleaved back by disulfide reductase to restore free thiolate. This rescue loop would thus prevent irreversible thiol hyperoxidation. Using dimedone switch method we could first show that persulfidation is a posttranslational modification that is evolutionarily conserved (bacteria, flies, worms, mice and humans). Then we could also observe that thioredoxin readily catalyzes the reduction of cysteine S-sulfonate, two orders of magnitude faster than the reduction of corresponding disulfide. The persulfidation levels/H2S producing ability positively correlated with the ability of yeast, worms and mammalian cells to resist stress caused by ROS. Persulfidation declines with aging in worms, rats and mice, due to the downregulation of H2S producing enzymes, but dietary restriction in mice and worms restores persulfide levels and improves their lifespan. Based on these data we suggest that persulfidation represents an evolutionarily conserved anti-aging mechanism.",
publisher = "Berlin: Freie Universität Berlin",
journal = "SPP1710 Conference: Thiol-based switches and redox regulation - from microbes to men; 2019 Sep 15-20; Sant Feliu de Guixols, Spain",
title = "Protein Persulfidation Protects Against Aging",
pages = "169-169",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6039"
}

Živanović, J., Kouroussis, E., Kohl, J., Miler, M., Mitchell, S., Mitchell, J., Schwartz, G., Milošević, V.,& Filipović R., M.. (2019). Protein Persulfidation Protects Against Aging. in SPP1710 Conference: Thiol-based switches and redox regulation - from microbes to men; 2019 Sep 15-20; Sant Feliu de Guixols, Spain
Berlin: Freie Universität Berlin., 169-169.
https://hdl.handle.net/21.15107/rcub_ibiss_6039

Živanović J, Kouroussis E, Kohl J, Miler M, Mitchell S, Mitchell J, Schwartz G, Milošević V, Filipović R. M. Protein Persulfidation Protects Against Aging. in SPP1710 Conference: Thiol-based switches and redox regulation - from microbes to men; 2019 Sep 15-20; Sant Feliu de Guixols, Spain. 2019;:169-169.
https://hdl.handle.net/21.15107/rcub_ibiss_6039 .

Živanović, Jasmina, Kouroussis, Emilia, Kohl, Joshua, Miler, Marko, Mitchell, Sarah, Mitchell, James, Schwartz, Guenter, Milošević, Verica, Filipović R., Miloš, "Protein Persulfidation Protects Against Aging" in SPP1710 Conference: Thiol-based switches and redox regulation - from microbes to men; 2019 Sep 15-20; Sant Feliu de Guixols, Spain (2019):169-169,
https://hdl.handle.net/21.15107/rcub_ibiss_6039 .