TY  - JOUR
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Kovačević, Sanja
AU  - Vidović, Nevena
AU  - Đorđević, Ana
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
PY  - 2024
UR  - https://www.mdpi.com/1422-0067/25/16/8918
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6984
AB  - Oxidative stress and inflammation are significant causes of aging. At the same time, citrus flavanones, naringenin (NAR), and hesperetin (HES) are bioactives with proven antioxidant and anti-inflammatory properties. Nevertheless, there are still no data about flavanone’s influence and its potential effects on the healthy aging process and improving pituitary functioning. Thus, using qPCR, immunoblot, histological techniques, and biochemical assays, our study aimed to elucidate how citrus flavanones (15 mg/kg b.m. per os) affect antioxidant defense, inflammation, and stress hormone output in the old rat model. Our results showed that HES restores the redox environment in the pituitary by down-regulating the nuclear factor erythroid 2-related factor 2 (Nrf2) protein while increasing kelch-like ECH-associated protein 1 (Keap1), thioredoxin reductase (TrxR1), and superoxide dismutase 2 (SOD2) protein expression. Immunofluorescent analysis confirmed Nrf2 and Keap1 down- and up-regulation, respectively. Supplementation with NAR increased Keap1, Trxr1, glutathione peroxidase (Gpx), and glutathione reductase (Gr) mRNA expression. Decreased oxidative stress aligned with NLRP3 decrement after both flavanones and glycogen synthase kinase-3 (GSK3) only after HES. The signal intensity of adrenocorticotropic hormone (ACTH) cells did not change, while corticosterone levels in serum decreased after both flavanones. HES showed higher potential than NAR in affecting a redox environment without increasing the inflammatory response, while a decrease in corticosterone level has a solid link to longevity. Our findings suggest that HES could improve and facilitate redox and inflammatory dysregulation in the rat’s old pituitary.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Citrus Flavanone Effects on the Nrf2-Keap1/GSK3/NF-κB/NLRP3 Regulation and Corticotroph-Stress Hormone Loop in the Old Pituitary
IS  - 16
VL  - 25
DO  - 10.3390/ijms25168918
SP  - 8918
ER  - 

@article{
author = "Miler, Marko and Živanović, Jasmina and Kovačević, Sanja and Vidović, Nevena and Đorđević, Ana and Filipović, Branko and Ajdžanović, Vladimir",
year = "2024",
abstract = "Oxidative stress and inflammation are significant causes of aging. At the same time, citrus flavanones, naringenin (NAR), and hesperetin (HES) are bioactives with proven antioxidant and anti-inflammatory properties. Nevertheless, there are still no data about flavanone’s influence and its potential effects on the healthy aging process and improving pituitary functioning. Thus, using qPCR, immunoblot, histological techniques, and biochemical assays, our study aimed to elucidate how citrus flavanones (15 mg/kg b.m. per os) affect antioxidant defense, inflammation, and stress hormone output in the old rat model. Our results showed that HES restores the redox environment in the pituitary by down-regulating the nuclear factor erythroid 2-related factor 2 (Nrf2) protein while increasing kelch-like ECH-associated protein 1 (Keap1), thioredoxin reductase (TrxR1), and superoxide dismutase 2 (SOD2) protein expression. Immunofluorescent analysis confirmed Nrf2 and Keap1 down- and up-regulation, respectively. Supplementation with NAR increased Keap1, Trxr1, glutathione peroxidase (Gpx), and glutathione reductase (Gr) mRNA expression. Decreased oxidative stress aligned with NLRP3 decrement after both flavanones and glycogen synthase kinase-3 (GSK3) only after HES. The signal intensity of adrenocorticotropic hormone (ACTH) cells did not change, while corticosterone levels in serum decreased after both flavanones. HES showed higher potential than NAR in affecting a redox environment without increasing the inflammatory response, while a decrease in corticosterone level has a solid link to longevity. Our findings suggest that HES could improve and facilitate redox and inflammatory dysregulation in the rat’s old pituitary.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Citrus Flavanone Effects on the Nrf2-Keap1/GSK3/NF-κB/NLRP3 Regulation and Corticotroph-Stress Hormone Loop in the Old Pituitary",
number = "16",
volume = "25",
doi = "10.3390/ijms25168918",
pages = "8918"
}

Miler, M., Živanović, J., Kovačević, S., Vidović, N., Đorđević, A., Filipović, B.,& Ajdžanović, V.. (2024). Citrus Flavanone Effects on the Nrf2-Keap1/GSK3/NF-κB/NLRP3 Regulation and Corticotroph-Stress Hormone Loop in the Old Pituitary. in International Journal of Molecular Sciences
Basel: MDPI., 25(16), 8918.
https://doi.org/10.3390/ijms25168918

Miler M, Živanović J, Kovačević S, Vidović N, Đorđević A, Filipović B, Ajdžanović V. Citrus Flavanone Effects on the Nrf2-Keap1/GSK3/NF-κB/NLRP3 Regulation and Corticotroph-Stress Hormone Loop in the Old Pituitary. in International Journal of Molecular Sciences. 2024;25(16):8918.
doi:10.3390/ijms25168918 .

Miler, Marko, Živanović, Jasmina, Kovačević, Sanja, Vidović, Nevena, Đorđević, Ana, Filipović, Branko, Ajdžanović, Vladimir, "Citrus Flavanone Effects on the Nrf2-Keap1/GSK3/NF-κB/NLRP3 Regulation and Corticotroph-Stress Hormone Loop in the Old Pituitary" in International Journal of Molecular Sciences, 25, no. 16 (2024):8918,
https://doi.org/10.3390/ijms25168918 . .

TY  - JOUR
AU  - Vidović, Nevena
AU  - Ranković, Slavica
AU  - Oreščanin Dušić, Zorana
AU  - Debeljak-Martačić, Jasmina
AU  - Popović, Tamara
AU  - Tomić, Mirko
AU  - Glibetić, Marija
PY  - 2020
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0042-84501800122V
UR  - https://radar.ibiss.bg.ac.rs/123456789/3878
AB  - Background/Aim. Recently, there has been an increased interest in novel dietary antioxidants, including omega-3 fatty acids and bioactive proteins present in milk. The aim of this study was to examine potential antioxidant effects of four-weeks long fish-based and milk-based diets in female Wistar rats. Methods. Four-months old rats were divided into three groups receiving either: control diet, diet enriched with fish meal, or diet enriched with milk. The activities of antioxidant enzymes: glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and concentration of thiobarbituric acid reactive substances (TBARS) were determined in liver homogenates obtained at the end of the treatment period. Results. Statistically significant higher activities of GPx (3.52 ± 0.73 U/mg) and CAT (147.25 ± 15.93 U/mg) were detected in rats fed with fishbased meal in comparison with both the control (GPx: 1.93 ± 0.11 U/mg; CAT: 99.37 ± 10.03 U/mg) and the group fed with milk-based diet (GPx: 1.72 ± 0.52 U/mg; CAT: 104.18 ± 37.49 U/mg). Despite somewhat lower concentration of TBARS in the milk-treated group (0.88 ± 0.23 nmoL/mg), no significant differences were detected in comparison with other groups (the control group: 1.00 ± 0.08 nmoL/mg; the fish-based diet group: 1.13 ± 0.15 nmoL/mg). Conclusion. Diet enriched with fish could improve one’s oxidative status by enhancing activities of antioxidant enzymes in the liver tissue. On the contrary, we failed to obtain results suggesting that milk could serve as a source of dietary antioxidants
PB  - National Library of Serbia
T2  - Vojnosanitetski pregled
T1  - The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study
IS  - 6
VL  - 77
DO  - 10.2298/VSP180326122V
SP  - 641
EP  - 646
ER  - 

@article{
author = "Vidović, Nevena and Ranković, Slavica and Oreščanin Dušić, Zorana and Debeljak-Martačić, Jasmina and Popović, Tamara and Tomić, Mirko and Glibetić, Marija",
year = "2020",
abstract = "Background/Aim. Recently, there has been an increased interest in novel dietary antioxidants, including omega-3 fatty acids and bioactive proteins present in milk. The aim of this study was to examine potential antioxidant effects of four-weeks long fish-based and milk-based diets in female Wistar rats. Methods. Four-months old rats were divided into three groups receiving either: control diet, diet enriched with fish meal, or diet enriched with milk. The activities of antioxidant enzymes: glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and concentration of thiobarbituric acid reactive substances (TBARS) were determined in liver homogenates obtained at the end of the treatment period. Results. Statistically significant higher activities of GPx (3.52 ± 0.73 U/mg) and CAT (147.25 ± 15.93 U/mg) were detected in rats fed with fishbased meal in comparison with both the control (GPx: 1.93 ± 0.11 U/mg; CAT: 99.37 ± 10.03 U/mg) and the group fed with milk-based diet (GPx: 1.72 ± 0.52 U/mg; CAT: 104.18 ± 37.49 U/mg). Despite somewhat lower concentration of TBARS in the milk-treated group (0.88 ± 0.23 nmoL/mg), no significant differences were detected in comparison with other groups (the control group: 1.00 ± 0.08 nmoL/mg; the fish-based diet group: 1.13 ± 0.15 nmoL/mg). Conclusion. Diet enriched with fish could improve one’s oxidative status by enhancing activities of antioxidant enzymes in the liver tissue. On the contrary, we failed to obtain results suggesting that milk could serve as a source of dietary antioxidants",
publisher = "National Library of Serbia",
journal = "Vojnosanitetski pregled",
title = "The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study",
number = "6",
volume = "77",
doi = "10.2298/VSP180326122V",
pages = "641-646"
}

Vidović, N., Ranković, S., Oreščanin Dušić, Z., Debeljak-Martačić, J., Popović, T., Tomić, M.,& Glibetić, M.. (2020). The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study. in Vojnosanitetski pregled
National Library of Serbia., 77(6), 641-646.
https://doi.org/10.2298/VSP180326122V

Vidović N, Ranković S, Oreščanin Dušić Z, Debeljak-Martačić J, Popović T, Tomić M, Glibetić M. The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study. in Vojnosanitetski pregled. 2020;77(6):641-646.
doi:10.2298/VSP180326122V .

Vidović, Nevena, Ranković, Slavica, Oreščanin Dušić, Zorana, Debeljak-Martačić, Jasmina, Popović, Tamara, Tomić, Mirko, Glibetić, Marija, "The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study" in Vojnosanitetski pregled, 77, no. 6 (2020):641-646,
https://doi.org/10.2298/VSP180326122V . .