TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Belij, Sandra
AU  - Subota, Vesna S
AU  - Zolotarevski, Lidija D
AU  - Mirkov, Ivana
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1061
AB  - Warfarin is a Vitamin K (VK) antagonist that affects Vitamin K-dependent (VKD) processes, including blood coagulation, as well as processes unrelated to hemostasis such as bone growth, calcification, and growth of some cell types. In addition, warfarin exerts influence on some non-VKD-related activities, including anti-tumor and immunomodulating activity. With respect to the latter, both immune stimulating and suppressive effects have been noted in different experimental systems. To explore the in vivo immunomodulatory potential of warfarin on one type of activity (i.e., cytokine production) in two different immune cell populations (i.e., mononuclear or polymorphonuclear cells), effects of subchronic oral warfarin intake in rats on pro-inflammatory cytokine (i.e., TNF alpha, IL-6) production by peripheral blood mononuclear and polymorphonuclear cells (granulocytes) was examined. Differential effects of warfarin intake on TNF alpha and IL-6 were noted, depending on the type of peripheral blood leukocytes and on the cytokine examined. Specifically, a lack of effect on TNF alpha and a priming of IL-6 production by mononuclear cells along with a decrease in TNF alpha and a lack of effect on IL-6 in polymorphonuclear cells were seen in warfarin-exposed hosts. The cell-and cytokine-dependent effects from subchronic oral warfarin intake on peripheral blood leukocytes demonstrated in this study could, possibly, differentially affect reactions mediated by these cells. Ultimately, the observed effects in rats might have implications for those humans who are on long-term/prolonged warfarin therapy.
T2  - Journal of Immunotoxicology
T1  - Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats
IS  - 1
VL  - 10
SP  - 375
EP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1061
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Belij, Sandra and Subota, Vesna S and Zolotarevski, Lidija D and Mirkov, Ivana and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2013",
abstract = "Warfarin is a Vitamin K (VK) antagonist that affects Vitamin K-dependent (VKD) processes, including blood coagulation, as well as processes unrelated to hemostasis such as bone growth, calcification, and growth of some cell types. In addition, warfarin exerts influence on some non-VKD-related activities, including anti-tumor and immunomodulating activity. With respect to the latter, both immune stimulating and suppressive effects have been noted in different experimental systems. To explore the in vivo immunomodulatory potential of warfarin on one type of activity (i.e., cytokine production) in two different immune cell populations (i.e., mononuclear or polymorphonuclear cells), effects of subchronic oral warfarin intake in rats on pro-inflammatory cytokine (i.e., TNF alpha, IL-6) production by peripheral blood mononuclear and polymorphonuclear cells (granulocytes) was examined. Differential effects of warfarin intake on TNF alpha and IL-6 were noted, depending on the type of peripheral blood leukocytes and on the cytokine examined. Specifically, a lack of effect on TNF alpha and a priming of IL-6 production by mononuclear cells along with a decrease in TNF alpha and a lack of effect on IL-6 in polymorphonuclear cells were seen in warfarin-exposed hosts. The cell-and cytokine-dependent effects from subchronic oral warfarin intake on peripheral blood leukocytes demonstrated in this study could, possibly, differentially affect reactions mediated by these cells. Ultimately, the observed effects in rats might have implications for those humans who are on long-term/prolonged warfarin therapy.",
journal = "Journal of Immunotoxicology",
title = "Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats",
number = "1",
volume = "10",
pages = "375-24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1061"
}

Popov Aleksandrov, A., Belij, S., Subota, V. S., Zolotarevski, L. D., Mirkov, I., Kataranovski, D. S.,& Kataranovski, M.. (2013). Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats. in Journal of Immunotoxicology, 10(1), 375-24.
https://hdl.handle.net/21.15107/rcub_ibiss_1061

Popov Aleksandrov A, Belij S, Subota VS, Zolotarevski LD, Mirkov I, Kataranovski DS, Kataranovski M. Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats. in Journal of Immunotoxicology. 2013;10(1):375-24.
https://hdl.handle.net/21.15107/rcub_ibiss_1061 .

Popov Aleksandrov, Aleksandra, Belij, Sandra, Subota, Vesna S, Zolotarevski, Lidija D, Mirkov, Ivana, Kataranovski, Dragan S., Kataranovski, Milena, "Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats" in Journal of Immunotoxicology, 10, no. 1 (2013):375-24,
https://hdl.handle.net/21.15107/rcub_ibiss_1061 .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Vasilijić, Sasa R
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/961
AB  - Allergic contact dermatitis (ACD) is a common skin inflammatory disease that develops in hosts sensitized with contact allergens. Elucidation of dose-response relationships represents one of the approaches in studying the type of ACD in humans/animal models, termed as contact hyper-sensitivity reaction (CHS). Such studies have demonstrated that the intensity of sensitization determines the response to elicitation with a contact allergen, but underlying mechanisms are unclear. The aim of this study is to explore the impact of the sensitization on contact hypersensitivity to dinitrochlorobenzene (DNCB) in rats by measuring the incidence and intensity of a challenge response in hosts sensitized with two different doses (i.e. low and high) of this hapten. Assumptions concerning the contribution from the magnitude of sensitization doses were drawn on the basis of effects from the two doses on the measured reaction parameters. Ear swelling and activity of lymph nodes that drain challenged skin (cdLN), including cellularity, proliferation, and effector cytokine IFN gamma and IL-17 production was measured in rats sensitized with 0.4% or 4% DNCB and challenged with a non-irritant (0.13%) dose. Sensitization with 4% DNCB resulted in a greater proportion of rats who responded more intensely (than unsensitized challenged rats) to challenge in terms of ear swelling and increases in cdLN activity (except for IFN gamma). The intensity of cdLN responses was higher in these hosts as well. Among the high-dose-sensitized rats, greater cellularity/proliferation of cells from lymph nodes (sdLN) that drain the high-dose-sensitized skin, as well as higher IL-17 production, was noted compared to what was seen in rats that received low-dose sensitization. In contrast, unchanged spontaneous and even decreased hapten-stimulated IFN gamma production after the high DNCB dose was observed. Based on the data, it seems the impact of magnitude of sensitization dose on CHS might be related to the rise in the proportion of rats that responded to challenge with an increase of dLN activity. Coincidental higher production of IL-17 by dLN cells from the high-dose-sensitized rats and following challenge of these hosts underscored the significance of IL-17 for a CHS to DNCB.
T2  - Journal of Immunotoxicology
T1  - Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats
IS  - 4
VL  - 10
SP  - 165
EP  - 360
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_961
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Vasilijić, Sasa R and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2013",
abstract = "Allergic contact dermatitis (ACD) is a common skin inflammatory disease that develops in hosts sensitized with contact allergens. Elucidation of dose-response relationships represents one of the approaches in studying the type of ACD in humans/animal models, termed as contact hyper-sensitivity reaction (CHS). Such studies have demonstrated that the intensity of sensitization determines the response to elicitation with a contact allergen, but underlying mechanisms are unclear. The aim of this study is to explore the impact of the sensitization on contact hypersensitivity to dinitrochlorobenzene (DNCB) in rats by measuring the incidence and intensity of a challenge response in hosts sensitized with two different doses (i.e. low and high) of this hapten. Assumptions concerning the contribution from the magnitude of sensitization doses were drawn on the basis of effects from the two doses on the measured reaction parameters. Ear swelling and activity of lymph nodes that drain challenged skin (cdLN), including cellularity, proliferation, and effector cytokine IFN gamma and IL-17 production was measured in rats sensitized with 0.4% or 4% DNCB and challenged with a non-irritant (0.13%) dose. Sensitization with 4% DNCB resulted in a greater proportion of rats who responded more intensely (than unsensitized challenged rats) to challenge in terms of ear swelling and increases in cdLN activity (except for IFN gamma). The intensity of cdLN responses was higher in these hosts as well. Among the high-dose-sensitized rats, greater cellularity/proliferation of cells from lymph nodes (sdLN) that drain the high-dose-sensitized skin, as well as higher IL-17 production, was noted compared to what was seen in rats that received low-dose sensitization. In contrast, unchanged spontaneous and even decreased hapten-stimulated IFN gamma production after the high DNCB dose was observed. Based on the data, it seems the impact of magnitude of sensitization dose on CHS might be related to the rise in the proportion of rats that responded to challenge with an increase of dLN activity. Coincidental higher production of IL-17 by dLN cells from the high-dose-sensitized rats and following challenge of these hosts underscored the significance of IL-17 for a CHS to DNCB.",
journal = "Journal of Immunotoxicology",
title = "Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats",
number = "4",
volume = "10",
pages = "165-360",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_961"
}

Popov Aleksandrov, A., Mirkov, I., Vasilijić, S. R., Zolotarevski, L. D., Kataranovski, D. S.,& Kataranovski, M.. (2013). Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats. in Journal of Immunotoxicology, 10(4), 165-360.
https://hdl.handle.net/21.15107/rcub_ibiss_961

Popov Aleksandrov A, Mirkov I, Vasilijić SR, Zolotarevski LD, Kataranovski DS, Kataranovski M. Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats. in Journal of Immunotoxicology. 2013;10(4):165-360.
https://hdl.handle.net/21.15107/rcub_ibiss_961 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Vasilijić, Sasa R, Zolotarevski, Lidija D, Kataranovski, Dragan S., Kataranovski, Milena, "Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats" in Journal of Immunotoxicology, 10, no. 4 (2013):165-360,
https://hdl.handle.net/21.15107/rcub_ibiss_961 .

TY  - JOUR
AU  - Glamočlija, Jasmina
AU  - Mirkov, Ivana
AU  - Kataranovski, Milena
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Stošić-Grujičić, Stanislava
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1039
AB  - Mirkov I, Glamoclija J, Stosic-Grujicic S, Zolotarevski L, Kataranovski D, Kataranovski M. Differential strain-related tissue immune response to sublethal systemic Aspergillus fumigatus infection in mice. APMIS 2013; 121: 211-20. Using a nonlethal systemic Aspergillus fumigatus infection, we have recently shown that similarly efficient elimination of fungus from spleens of prototypic Th1 (C57BL/6) and prototypic Th2 (BALB/c) mice is associated with differential immune responses. In light of these data and given the disseminated character of infection, the aim of the present study is to explore whether there are also strain-dependent differences in antifungal responses in peripheral tissues of infected mice. Although similar efficiency of conidia removal was noted in liver and kidneys of both strains, BALB/c mice seemed more prone to tissue injury. Compared with other nonlymphoid organs, lungs proved immunologically the most responsive in systemic aspergillosis. Lower numbers of neutrophils and macrophages in the lungs of infected BALB/c mice, delayed and lower (compared with C57BL/6 mice) expression of their oxidative activity, along with late IFN- and upregulated IL-4 production by lung cells might be responsible for slower elimination of A. fumigatus from the lungs of this mouse strain. The data obtained imply that lungs should be viewed as mandatory organ in evaluation of immune-mediated antifungal potential of drugs in models of systemic/disseminated infection and that strain differences noted in tissue responses should be taken into account in these settings.
T2  - Apmis
T1  - Differential strain-related tissue immune response to sublethal systemic Aspergillus fumigatus infection in mice
IS  - 3
VL  - 121
SP  - 493
EP  - 220
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1039
ER  - 

@article{
author = "Glamočlija, Jasmina and Mirkov, Ivana and Kataranovski, Milena and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Stošić-Grujičić, Stanislava",
year = "2013",
abstract = "Mirkov I, Glamoclija J, Stosic-Grujicic S, Zolotarevski L, Kataranovski D, Kataranovski M. Differential strain-related tissue immune response to sublethal systemic Aspergillus fumigatus infection in mice. APMIS 2013; 121: 211-20. Using a nonlethal systemic Aspergillus fumigatus infection, we have recently shown that similarly efficient elimination of fungus from spleens of prototypic Th1 (C57BL/6) and prototypic Th2 (BALB/c) mice is associated with differential immune responses. In light of these data and given the disseminated character of infection, the aim of the present study is to explore whether there are also strain-dependent differences in antifungal responses in peripheral tissues of infected mice. Although similar efficiency of conidia removal was noted in liver and kidneys of both strains, BALB/c mice seemed more prone to tissue injury. Compared with other nonlymphoid organs, lungs proved immunologically the most responsive in systemic aspergillosis. Lower numbers of neutrophils and macrophages in the lungs of infected BALB/c mice, delayed and lower (compared with C57BL/6 mice) expression of their oxidative activity, along with late IFN- and upregulated IL-4 production by lung cells might be responsible for slower elimination of A. fumigatus from the lungs of this mouse strain. The data obtained imply that lungs should be viewed as mandatory organ in evaluation of immune-mediated antifungal potential of drugs in models of systemic/disseminated infection and that strain differences noted in tissue responses should be taken into account in these settings.",
journal = "Apmis",
title = "Differential strain-related tissue immune response to sublethal systemic Aspergillus fumigatus infection in mice",
number = "3",
volume = "121",
pages = "493-220",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1039"
}

Glamočlija, J., Mirkov, I., Kataranovski, M., Zolotarevski, L. D., Kataranovski, D. S.,& Stošić-Grujičić, S.. (2013). Differential strain-related tissue immune response to sublethal systemic Aspergillus fumigatus infection in mice. in Apmis, 121(3), 493-220.
https://hdl.handle.net/21.15107/rcub_ibiss_1039

Glamočlija J, Mirkov I, Kataranovski M, Zolotarevski LD, Kataranovski DS, Stošić-Grujičić S. Differential strain-related tissue immune response to sublethal systemic Aspergillus fumigatus infection in mice. in Apmis. 2013;121(3):493-220.
https://hdl.handle.net/21.15107/rcub_ibiss_1039 .

Glamočlija, Jasmina, Mirkov, Ivana, Kataranovski, Milena, Zolotarevski, Lidija D, Kataranovski, Dragan S., Stošić-Grujičić, Stanislava, "Differential strain-related tissue immune response to sublethal systemic Aspergillus fumigatus infection in mice" in Apmis, 121, no. 3 (2013):493-220,
https://hdl.handle.net/21.15107/rcub_ibiss_1039 .

TY  - CONF
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Miljković, Đorđe
AU  - Belij, Sandra
AU  - Đokić, Jelena
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1113
C3  - Immunology
T1  - Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats
IS  - null
VL  - 137
SP  - 349
EP  - 442
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1113
ER  - 

@conference{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Miljković, Đorđe and Belij, Sandra and Đokić, Jelena and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2012",
journal = "Immunology",
title = "Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats",
number = "null",
volume = "137",
pages = "349-442",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1113"
}

Popov Aleksandrov, A., Mirkov, I., Miljković, Đ., Belij, S., Đokić, J., Zolotarevski, L. D., Kataranovski, D. S.,& Kataranovski, M.. (2012). Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats. in Immunology, 137(null), 349-442.
https://hdl.handle.net/21.15107/rcub_ibiss_1113

Popov Aleksandrov A, Mirkov I, Miljković Đ, Belij S, Đokić J, Zolotarevski LD, Kataranovski DS, Kataranovski M. Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats. in Immunology. 2012;137(null):349-442.
https://hdl.handle.net/21.15107/rcub_ibiss_1113 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Miljković, Đorđe, Belij, Sandra, Đokić, Jelena, Zolotarevski, Lidija D, Kataranovski, Dragan S., Kataranovski, Milena, "Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats" in Immunology, 137, no. null (2012):349-442,
https://hdl.handle.net/21.15107/rcub_ibiss_1113 .

TY  - JOUR
AU  - Belij, Sandra
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1207
AB  - Contact hypersensitivity reaction (CHS) is a T-cell-mediated skin inflammatory reaction to cutaneous exposure to small sensitizing chemicals, haptens. While the significance of local inflammatory skin response to the hapten application in CHS induction and expression is known, there is paucity of data concerning systemic inflammation in CHS. In this study, changes in cellular (peripheral blood granulocytes) and humoral (plasma tumor necrosis factor (TNF)-alpha levels) components of inflammation during sensitization of rats with two consecutive applications of dinitrochlorobenzene (DNCB) were examined. The impact of sensitization on these parameters was determined by employing low (0.4%) and high (4%) hapten doses and by examining the dynamics (i.e. one and three days following the last application of DNCB) of these changes. Dose-dependent increase in relative numbers and priming (for respiratory burst and adhesion) effect of skin sensitization with DNCB on peripheral blood neutrophils in rats were noted. No changes in circulating TNF-alpha levels were observed following the sensitization. The increase in lung myeloperoxidase content and histologically evident presence of neutrophils was observed in lungs of the sensitized rats. The changes in granulocyte priming for adhesion might have accounted for the observed increase in lung neutrophil content in the sensitized rats.
T2  - Cutaneous and Ocular Toxicology
T1  - Percutaneous toxicity of dinitrochlorobenzene (DNCB) in rats
IS  - 1
VL  - 31
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1207
ER  - 

@article{
author = "Belij, Sandra and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2012",
abstract = "Contact hypersensitivity reaction (CHS) is a T-cell-mediated skin inflammatory reaction to cutaneous exposure to small sensitizing chemicals, haptens. While the significance of local inflammatory skin response to the hapten application in CHS induction and expression is known, there is paucity of data concerning systemic inflammation in CHS. In this study, changes in cellular (peripheral blood granulocytes) and humoral (plasma tumor necrosis factor (TNF)-alpha levels) components of inflammation during sensitization of rats with two consecutive applications of dinitrochlorobenzene (DNCB) were examined. The impact of sensitization on these parameters was determined by employing low (0.4%) and high (4%) hapten doses and by examining the dynamics (i.e. one and three days following the last application of DNCB) of these changes. Dose-dependent increase in relative numbers and priming (for respiratory burst and adhesion) effect of skin sensitization with DNCB on peripheral blood neutrophils in rats were noted. No changes in circulating TNF-alpha levels were observed following the sensitization. The increase in lung myeloperoxidase content and histologically evident presence of neutrophils was observed in lungs of the sensitized rats. The changes in granulocyte priming for adhesion might have accounted for the observed increase in lung neutrophil content in the sensitized rats.",
journal = "Cutaneous and Ocular Toxicology",
title = "Percutaneous toxicity of dinitrochlorobenzene (DNCB) in rats",
number = "1",
volume = "31",
pages = "13",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1207"
}

Belij, S., Popov Aleksandrov, A., Mirkov, I., Zolotarevski, L. D., Kataranovski, D. S.,& Kataranovski, M.. (2012). Percutaneous toxicity of dinitrochlorobenzene (DNCB) in rats. in Cutaneous and Ocular Toxicology, 31(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1207

Belij S, Popov Aleksandrov A, Mirkov I, Zolotarevski LD, Kataranovski DS, Kataranovski M. Percutaneous toxicity of dinitrochlorobenzene (DNCB) in rats. in Cutaneous and Ocular Toxicology. 2012;31(1):null-13.
https://hdl.handle.net/21.15107/rcub_ibiss_1207 .

Belij, Sandra, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Zolotarevski, Lidija D, Kataranovski, Dragan S., Kataranovski, Milena, "Percutaneous toxicity of dinitrochlorobenzene (DNCB) in rats" in Cutaneous and Ocular Toxicology, 31, no. 1 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1207 .

TY  - JOUR
AU  - Belij, Sandra
AU  - Popov Aleksandrov, Aleksandra
AU  - Zolotarevski, Lidija D
AU  - Mirkov, Ivana
AU  - Đokić, Jelena
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1203
AB  - Topical application of dinitrochlorobenzene (DNCB) is employed in the immunotherapy of skin diseases. Activation of T-cell mediated immune responses (Th1/type1) is the supposed mechanism of the clinical effect of DNCB, but there are no data concerning innate/inflammatory mechanisms. In this study, the effect of repeated topical DNCB application on peripheral blood polymorphonuclear (PMN) leukocytes has been examined in two rat strains which differ in the propensity to mount Th1/type1 or Th2/type2 responses. The dynamics of changes in PMN numbers and effector activities (respiratory burst, nitric oxide production and myeloperoxidase content), as well as in adhesion and TNF-alpha production following the rat skin sensitization with low (0.4%) and high (4%) DNCB doses were measured. Both priming and activation of PMNs were observed following skin sensitization with DNCB, with dose-dependent as well as time-dependent differences in some PMN activities. Obtained data might be relevant for understanding the immune mechanisms of topical DNCB therapy. (C) 2011 Published by Elsevier B.V.
T2  - Environmental Toxicology and Pharmacology
T1  - Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells
IS  - 2
VL  - 33
EP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1203
ER  - 

@article{
author = "Belij, Sandra and Popov Aleksandrov, Aleksandra and Zolotarevski, Lidija D and Mirkov, Ivana and Đokić, Jelena and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2012",
abstract = "Topical application of dinitrochlorobenzene (DNCB) is employed in the immunotherapy of skin diseases. Activation of T-cell mediated immune responses (Th1/type1) is the supposed mechanism of the clinical effect of DNCB, but there are no data concerning innate/inflammatory mechanisms. In this study, the effect of repeated topical DNCB application on peripheral blood polymorphonuclear (PMN) leukocytes has been examined in two rat strains which differ in the propensity to mount Th1/type1 or Th2/type2 responses. The dynamics of changes in PMN numbers and effector activities (respiratory burst, nitric oxide production and myeloperoxidase content), as well as in adhesion and TNF-alpha production following the rat skin sensitization with low (0.4%) and high (4%) DNCB doses were measured. Both priming and activation of PMNs were observed following skin sensitization with DNCB, with dose-dependent as well as time-dependent differences in some PMN activities. Obtained data might be relevant for understanding the immune mechanisms of topical DNCB therapy. (C) 2011 Published by Elsevier B.V.",
journal = "Environmental Toxicology and Pharmacology",
title = "Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells",
number = "2",
volume = "33",
pages = "180",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1203"
}

Belij, S., Popov Aleksandrov, A., Zolotarevski, L. D., Mirkov, I., Đokić, J., Kataranovski, D. S.,& Kataranovski, M.. (2012). Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells. in Environmental Toxicology and Pharmacology, 33(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1203

Belij S, Popov Aleksandrov A, Zolotarevski LD, Mirkov I, Đokić J, Kataranovski DS, Kataranovski M. Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells. in Environmental Toxicology and Pharmacology. 2012;33(2):null-180.
https://hdl.handle.net/21.15107/rcub_ibiss_1203 .

Belij, Sandra, Popov Aleksandrov, Aleksandra, Zolotarevski, Lidija D, Mirkov, Ivana, Đokić, Jelena, Kataranovski, Dragan S., Kataranovski, Milena, "Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells" in Environmental Toxicology and Pharmacology, 33, no. 2 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1203 .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Belij, Sandra
AU  - Kataranovski, Milena
AU  - Zolotarevski, Lidija D
AU  - Glamočlija, Jasmina
AU  - Stojanović, Ivana D.
AU  - Stošić-Grujičić, Stanislava
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1170
AB  - We recently demonstrated that macrophage migration inhibitory factor deficient (MIF-/-) mice exhibited a higher susceptibility to lethal systemic Aspergillus fumigatus infections than genetically matched, wild-type (WT) C57BL/6 mice, and displayed altered cytokine profiles in the spleen when challenged by sublethal infections. In this report we focused on the potential involvement of MIF in the response of mice to sublethal systemic A. fumigatus infections in tissues other than spleen. Impaired fungal clearance from lungs, kidneys, liver and brain in MIF-/- mice was noted and was associated with histologically-evident differences in signs of inflammation in these organs. Higher values of some indicators of pathologic changes in urine parameters (increases in bilirubin, glucose and ketones), as well as a greater degree of brain tissue damage, pointed to multiple organs being affected in MIF-/- mice. Analysis of the lung response revealed differences in the composition of infiltrated cells between MIF-sufficient and MIF-deficient mice. MPO activity and reactive oxygen species production were impaired, as well as production of IL-17 and IFN-gamma in MIF-/- mice as compared to WT counterparts. Lower systemic IL-1 beta and IL-6 levels in infected MIF-/- mice coincided with reduced blood neutrophil counts and organ infiltration. Collectively, this study identifies MIF as a resistance factor that orchestrates events in several non-lymphoid areas which provide a milieu that accomplishes anti-fungal A. fumigatus defense.
T2  - Medical Mycology
T1  - The relevance of the migration inhibitory factor (MIF) for peripheral tissue response in murine sublethal systemic Aspergillus fumigatus infection
IS  - 5
VL  - 50
EP  - 487
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1170
ER  - 

@article{
author = "Mirkov, Ivana and Belij, Sandra and Kataranovski, Milena and Zolotarevski, Lidija D and Glamočlija, Jasmina and Stojanović, Ivana D. and Stošić-Grujičić, Stanislava",
year = "2012",
abstract = "We recently demonstrated that macrophage migration inhibitory factor deficient (MIF-/-) mice exhibited a higher susceptibility to lethal systemic Aspergillus fumigatus infections than genetically matched, wild-type (WT) C57BL/6 mice, and displayed altered cytokine profiles in the spleen when challenged by sublethal infections. In this report we focused on the potential involvement of MIF in the response of mice to sublethal systemic A. fumigatus infections in tissues other than spleen. Impaired fungal clearance from lungs, kidneys, liver and brain in MIF-/- mice was noted and was associated with histologically-evident differences in signs of inflammation in these organs. Higher values of some indicators of pathologic changes in urine parameters (increases in bilirubin, glucose and ketones), as well as a greater degree of brain tissue damage, pointed to multiple organs being affected in MIF-/- mice. Analysis of the lung response revealed differences in the composition of infiltrated cells between MIF-sufficient and MIF-deficient mice. MPO activity and reactive oxygen species production were impaired, as well as production of IL-17 and IFN-gamma in MIF-/- mice as compared to WT counterparts. Lower systemic IL-1 beta and IL-6 levels in infected MIF-/- mice coincided with reduced blood neutrophil counts and organ infiltration. Collectively, this study identifies MIF as a resistance factor that orchestrates events in several non-lymphoid areas which provide a milieu that accomplishes anti-fungal A. fumigatus defense.",
journal = "Medical Mycology",
title = "The relevance of the migration inhibitory factor (MIF) for peripheral tissue response in murine sublethal systemic Aspergillus fumigatus infection",
number = "5",
volume = "50",
pages = "487",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1170"
}

Mirkov, I., Belij, S., Kataranovski, M., Zolotarevski, L. D., Glamočlija, J., Stojanović, I. D.,& Stošić-Grujičić, S.. (2012). The relevance of the migration inhibitory factor (MIF) for peripheral tissue response in murine sublethal systemic Aspergillus fumigatus infection. in Medical Mycology, 50(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1170

Mirkov I, Belij S, Kataranovski M, Zolotarevski LD, Glamočlija J, Stojanović ID, Stošić-Grujičić S. The relevance of the migration inhibitory factor (MIF) for peripheral tissue response in murine sublethal systemic Aspergillus fumigatus infection. in Medical Mycology. 2012;50(5):null-487.
https://hdl.handle.net/21.15107/rcub_ibiss_1170 .

Mirkov, Ivana, Belij, Sandra, Kataranovski, Milena, Zolotarevski, Lidija D, Glamočlija, Jasmina, Stojanović, Ivana D., Stošić-Grujičić, Stanislava, "The relevance of the migration inhibitory factor (MIF) for peripheral tissue response in murine sublethal systemic Aspergillus fumigatus infection" in Medical Mycology, 50, no. 5 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1170 .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Zolotarevski, Lidija D
AU  - Jović, Milena
AU  - Belij, Sandra
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1299
AB  - Objective: To evaluate the effects of epicutaneous application of anticoagulant warfarin, by examining the presence of tissue injury and immune/inflammatory activity in exposed skin. Methods: Rats were exposed to warfarin by applying 10 mu g of warfarin-sodium to 10-12 cm(2) skin (range 0.8-1 mu g per 1 cm(2)) for 3 consecutive days. Tissue injury was evaluated by lipid peroxidation, histomorphological changes and signs of reparative activity in skin. T cell infiltration and selected aspects of epidermal cell activity were examined as indicators of immune/inflammatory skin response to warfarin application. Results: Repeated warfarin application exerted no effect on skin metabolic viability, but resulted in tissue injury (increased malondialdehyde, MDA, production, evident histo-morphological changes in epidermis and dermis depicting cell injury and death). Increased numbers of proliferating cell nuclear antigen (PCNA(+)) cells indicated reparative processes in injured skin. Infiltration of CD3(+) cells (T lymphocytes) along with the increased production of tumor necrosis factor-alpha (TNF-alpha) by epidermal cells from warfarin-treated skin and their co-stimulatory effect in an in vitro T-cell activation assay demonstrated immunomodulatory effects of epicutaneous warfarin. Conclusion: Presented data have documented tissue damage associated with immune/inflammatory activity in skin exposed to warfarin. Observed effects are relevant to immunotoxic potential of this anticoagulant in settings of external exposure.
T2  - Biomedical and Environmental Sciences
T1  - Local Proinflammatory Effects of Repeated Skin Exposure to Warfarin, An Anticoagulant Rodenticide in Rats
IS  - 2
VL  - 24
EP  - 189
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1299
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Zolotarevski, Lidija D and Jović, Milena and Belij, Sandra and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2011",
abstract = "Objective: To evaluate the effects of epicutaneous application of anticoagulant warfarin, by examining the presence of tissue injury and immune/inflammatory activity in exposed skin. Methods: Rats were exposed to warfarin by applying 10 mu g of warfarin-sodium to 10-12 cm(2) skin (range 0.8-1 mu g per 1 cm(2)) for 3 consecutive days. Tissue injury was evaluated by lipid peroxidation, histomorphological changes and signs of reparative activity in skin. T cell infiltration and selected aspects of epidermal cell activity were examined as indicators of immune/inflammatory skin response to warfarin application. Results: Repeated warfarin application exerted no effect on skin metabolic viability, but resulted in tissue injury (increased malondialdehyde, MDA, production, evident histo-morphological changes in epidermis and dermis depicting cell injury and death). Increased numbers of proliferating cell nuclear antigen (PCNA(+)) cells indicated reparative processes in injured skin. Infiltration of CD3(+) cells (T lymphocytes) along with the increased production of tumor necrosis factor-alpha (TNF-alpha) by epidermal cells from warfarin-treated skin and their co-stimulatory effect in an in vitro T-cell activation assay demonstrated immunomodulatory effects of epicutaneous warfarin. Conclusion: Presented data have documented tissue damage associated with immune/inflammatory activity in skin exposed to warfarin. Observed effects are relevant to immunotoxic potential of this anticoagulant in settings of external exposure.",
journal = "Biomedical and Environmental Sciences",
title = "Local Proinflammatory Effects of Repeated Skin Exposure to Warfarin, An Anticoagulant Rodenticide in Rats",
number = "2",
volume = "24",
pages = "189",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1299"
}

Popov Aleksandrov, A., Mirkov, I., Zolotarevski, L. D., Jović, M., Belij, S., Kataranovski, D. S.,& Kataranovski, M.. (2011). Local Proinflammatory Effects of Repeated Skin Exposure to Warfarin, An Anticoagulant Rodenticide in Rats. in Biomedical and Environmental Sciences, 24(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1299

Popov Aleksandrov A, Mirkov I, Zolotarevski LD, Jović M, Belij S, Kataranovski DS, Kataranovski M. Local Proinflammatory Effects of Repeated Skin Exposure to Warfarin, An Anticoagulant Rodenticide in Rats. in Biomedical and Environmental Sciences. 2011;24(2):null-189.
https://hdl.handle.net/21.15107/rcub_ibiss_1299 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Zolotarevski, Lidija D, Jović, Milena, Belij, Sandra, Kataranovski, Dragan S., Kataranovski, Milena, "Local Proinflammatory Effects of Repeated Skin Exposure to Warfarin, An Anticoagulant Rodenticide in Rats" in Biomedical and Environmental Sciences, 24, no. 2 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1299 .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Miljković, Đorđe
AU  - Belij, Sandra
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1278
AB  - Contact hypersensitivity (CHS) is a T-cell-mediated skin inflammatory reaction to cutaneous exposure to small sensitizing chemicals, haptens. Majority of CHS studies were conducted in mice and there is paucity of data in other experimental animals. In the present study, characteristics of contact hypersensitivity reaction to dinitrochlorobenzene (DNCB) were determined in Th1-prone Dark Agouti (DA) rats by evaluating sensitization phase as a function of time-dependent changes in draining lymph nodes (DLN). Apart from basic indices of DLN activity (cellularity and proliferation), the production of cytokines relevant for CHS induction, interleukin-6 (IL-6), interferon-gamma (IFN-gamma), interleukin-17 (IL-17) and interleukin-4 (IL-4) was analyzed. Anti-inflammatory cytokine interleukin-10 (IL-10) production by DLN cells was determined as well. Highest production of IL-6, IFN-gamma and IL-17 in sensitized animals was observed at day 3 after DNCB application, with a decrease at day 5. Increased messages for IFN-gamma and IL-17 were noted at this time point. In contrast to inflammatory cytokines, anti-inflammatory cytokine interleukin-4 (IL-4) was undetectable during the entire sensitization phase. Differential pattern (IL-6 and IFN-gamma) and level (IFN-gamma and IL-17) of inflammatory cytokine production was noted in sensitized Th2-prone Albino Oxford (AO) rats. Similarly to DA rats, no changes in IL-4 were noted in AO rats. Strain-dependent differences in inflammatory cytokine production seem to be based on anti-inflammatory cytokine interleukin-10 (IL-10). Production of IFN-gamma concomitantly with undetectable IL-4 in both strains classify rat CHS to DNCB as Th1/type 1 reaction. Detection of IL-17 in sensitized DLN cells points to the involvement of T(IL-17) cells in rat contact hypersensitivity. (C) 2011 Elsevier GmbH. All rights reserved.
T2  - Immunobiology
T1  - Contact allergic response to dinitrochlorobenzene (DNCB) in rats: Insight from sensitization phase
IS  - 7
VL  - 216
EP  - 770
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1278
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Miljković, Đorđe and Belij, Sandra and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2011",
abstract = "Contact hypersensitivity (CHS) is a T-cell-mediated skin inflammatory reaction to cutaneous exposure to small sensitizing chemicals, haptens. Majority of CHS studies were conducted in mice and there is paucity of data in other experimental animals. In the present study, characteristics of contact hypersensitivity reaction to dinitrochlorobenzene (DNCB) were determined in Th1-prone Dark Agouti (DA) rats by evaluating sensitization phase as a function of time-dependent changes in draining lymph nodes (DLN). Apart from basic indices of DLN activity (cellularity and proliferation), the production of cytokines relevant for CHS induction, interleukin-6 (IL-6), interferon-gamma (IFN-gamma), interleukin-17 (IL-17) and interleukin-4 (IL-4) was analyzed. Anti-inflammatory cytokine interleukin-10 (IL-10) production by DLN cells was determined as well. Highest production of IL-6, IFN-gamma and IL-17 in sensitized animals was observed at day 3 after DNCB application, with a decrease at day 5. Increased messages for IFN-gamma and IL-17 were noted at this time point. In contrast to inflammatory cytokines, anti-inflammatory cytokine interleukin-4 (IL-4) was undetectable during the entire sensitization phase. Differential pattern (IL-6 and IFN-gamma) and level (IFN-gamma and IL-17) of inflammatory cytokine production was noted in sensitized Th2-prone Albino Oxford (AO) rats. Similarly to DA rats, no changes in IL-4 were noted in AO rats. Strain-dependent differences in inflammatory cytokine production seem to be based on anti-inflammatory cytokine interleukin-10 (IL-10). Production of IFN-gamma concomitantly with undetectable IL-4 in both strains classify rat CHS to DNCB as Th1/type 1 reaction. Detection of IL-17 in sensitized DLN cells points to the involvement of T(IL-17) cells in rat contact hypersensitivity. (C) 2011 Elsevier GmbH. All rights reserved.",
journal = "Immunobiology",
title = "Contact allergic response to dinitrochlorobenzene (DNCB) in rats: Insight from sensitization phase",
number = "7",
volume = "216",
pages = "770",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1278"
}

Popov Aleksandrov, A., Mirkov, I., Miljković, Đ., Belij, S., Zolotarevski, L. D., Kataranovski, D. S.,& Kataranovski, M.. (2011). Contact allergic response to dinitrochlorobenzene (DNCB) in rats: Insight from sensitization phase. in Immunobiology, 216(7).
https://hdl.handle.net/21.15107/rcub_ibiss_1278

Popov Aleksandrov A, Mirkov I, Miljković Đ, Belij S, Zolotarevski LD, Kataranovski DS, Kataranovski M. Contact allergic response to dinitrochlorobenzene (DNCB) in rats: Insight from sensitization phase. in Immunobiology. 2011;216(7):null-770.
https://hdl.handle.net/21.15107/rcub_ibiss_1278 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Miljković, Đorđe, Belij, Sandra, Zolotarevski, Lidija D, Kataranovski, Dragan S., Kataranovski, Milena, "Contact allergic response to dinitrochlorobenzene (DNCB) in rats: Insight from sensitization phase" in Immunobiology, 216, no. 7 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1278 .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Stojanović, Ivana D.
AU  - Glamočlija, Jasmina
AU  - Stošić-Grujičić, Stanislava
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1327
AB  - Studies of systemic and pulmonary Aspergillus fumigatus infection demonstrated differential susceptibility of inbred mice of various genetic background to lethal outcome, with an opposite pattern of Th1 cytokine interferon-gamma (IFN-gamma) and Th2 cytokine interleukin-4 (IL-4) in susceptible vs resistant mice. We have shown recently reciprocal IFN-gamma and IL-4 expression in spleens of Th1 -prone C57BL/6 mice in sublethal systemic aspergillosis. In this study, resistance to systemic (i.v.) A. fumigatus infection was investigated in Th2-prone BALB/c mice by survival rate at different fungal inocula, efficiency of reduction of visceral organ and spleen fungal burden at sublethal conidia dose and splenic immune response to this dose and compared to C57BL/6 mice. No strain differences in survival were noted at three A. fumigatus doses, with similar extent and dynamics of fungal eradication from all organs following sublethal conidia dose injection. Progressive decrease in spleen fungal burden was associated with different dynamics and quality of changes in spleen activity of BALB/c and C57BL/6 mice. Increased spleen mass and cellularity was noted in both strains, with higher values in BALB/c mice at some time points what might be ascribed to peripheral blood cell recruitment, as well as hematopoietic activity and red pulp upgrowth. Infection tipped the balance towards pro-inflammatory antifungal splenic response by a highly increasing IFN-gamma and without changing the IL-4 expression in BALB/c mice, in contrast to down-regulating anti-inflammatory (IL-4) and a moderately increasing IFN-gamma response in C57BL/6 mice. Jointly, stimulation of IL-17 expression noted in both strains provided an optimal inflammatory milieu in the spleen of infected mice that might have contributed to efficient removal of conidia. (C) 2010 Elsevier GmbH. All rights reserved.
T2  - Immunobiology
T1  - Differential mechanisms of resistance to sublethal systemic Aspergillus fumigatus infection in immunocompetent BALB/c and C57BL/6 mice
IS  - 1-2
VL  - 216
EP  - 242
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1327
ER  - 

@article{
author = "Mirkov, Ivana and Stojanović, Ivana D. and Glamočlija, Jasmina and Stošić-Grujičić, Stanislava and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2011",
abstract = "Studies of systemic and pulmonary Aspergillus fumigatus infection demonstrated differential susceptibility of inbred mice of various genetic background to lethal outcome, with an opposite pattern of Th1 cytokine interferon-gamma (IFN-gamma) and Th2 cytokine interleukin-4 (IL-4) in susceptible vs resistant mice. We have shown recently reciprocal IFN-gamma and IL-4 expression in spleens of Th1 -prone C57BL/6 mice in sublethal systemic aspergillosis. In this study, resistance to systemic (i.v.) A. fumigatus infection was investigated in Th2-prone BALB/c mice by survival rate at different fungal inocula, efficiency of reduction of visceral organ and spleen fungal burden at sublethal conidia dose and splenic immune response to this dose and compared to C57BL/6 mice. No strain differences in survival were noted at three A. fumigatus doses, with similar extent and dynamics of fungal eradication from all organs following sublethal conidia dose injection. Progressive decrease in spleen fungal burden was associated with different dynamics and quality of changes in spleen activity of BALB/c and C57BL/6 mice. Increased spleen mass and cellularity was noted in both strains, with higher values in BALB/c mice at some time points what might be ascribed to peripheral blood cell recruitment, as well as hematopoietic activity and red pulp upgrowth. Infection tipped the balance towards pro-inflammatory antifungal splenic response by a highly increasing IFN-gamma and without changing the IL-4 expression in BALB/c mice, in contrast to down-regulating anti-inflammatory (IL-4) and a moderately increasing IFN-gamma response in C57BL/6 mice. Jointly, stimulation of IL-17 expression noted in both strains provided an optimal inflammatory milieu in the spleen of infected mice that might have contributed to efficient removal of conidia. (C) 2010 Elsevier GmbH. All rights reserved.",
journal = "Immunobiology",
title = "Differential mechanisms of resistance to sublethal systemic Aspergillus fumigatus infection in immunocompetent BALB/c and C57BL/6 mice",
number = "1-2",
volume = "216",
pages = "242",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1327"
}

Mirkov, I., Stojanović, I. D., Glamočlija, J., Stošić-Grujičić, S., Zolotarevski, L. D., Kataranovski, D. S.,& Kataranovski, M.. (2011). Differential mechanisms of resistance to sublethal systemic Aspergillus fumigatus infection in immunocompetent BALB/c and C57BL/6 mice. in Immunobiology, 216(1-2).
https://hdl.handle.net/21.15107/rcub_ibiss_1327

Mirkov I, Stojanović ID, Glamočlija J, Stošić-Grujičić S, Zolotarevski LD, Kataranovski DS, Kataranovski M. Differential mechanisms of resistance to sublethal systemic Aspergillus fumigatus infection in immunocompetent BALB/c and C57BL/6 mice. in Immunobiology. 2011;216(1-2):null-242.
https://hdl.handle.net/21.15107/rcub_ibiss_1327 .

Mirkov, Ivana, Stojanović, Ivana D., Glamočlija, Jasmina, Stošić-Grujičić, Stanislava, Zolotarevski, Lidija D, Kataranovski, Dragan S., Kataranovski, Milena, "Differential mechanisms of resistance to sublethal systemic Aspergillus fumigatus infection in immunocompetent BALB/c and C57BL/6 mice" in Immunobiology, 216, no. 1-2 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1327 .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Stojanović, Ivana D.
AU  - Stošić-Grujičić, Stanislava
AU  - Glamočlija, Jasmina
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1359
AB  - In this study, we investigated splenic and lung cell responses to nonlethal systemic Aspergillus fumigatus infection in mice. Apart from basic indices of spleen and lung cell activity, IL-17 expression by cells from both tissues was determined and compared to the expression of IFN-gamma and IL-4. Progressive decrease in tissue fungal burden correlated with increased spleen and lung cell activity. Increased IL-17 message was noted in spleen cells at all time points (3, 7 and 15 days post-infection; p.i.), while a modest increase in IFN-gamma mRNA expression was noted at day 3 p.i. Increased cytokine production at days 3 and 7 (IL-17) and throughout the experimental period (IFN-gamma) was found. In contrast, spleen cell IL-4 expression was considerably lower during infection, resulting in high IFN-gamma/IL-4 and IL-17/IL-4 ratios in the spleen. Pro-inflammatory cytokine response was observed in the lungs as well, but primarily as the result of increased production of IFN-gamma by lung cells in response to challenge with conidia and the absence of change in IL-4 response. Increased activity of cells from both tissues, as well as the pattern of cytokine production, created an optimal pro-inflammatory milieu for fungal eradication.
T2  - Medical Mycology
T1  - Splenic and lung response to nonlethal systemic Aspergillus fumigatus infection in C57BL/6 mice
IS  - 5
VL  - 48
EP  - 743
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1359
ER  - 

@article{
author = "Mirkov, Ivana and Stojanović, Ivana D. and Stošić-Grujičić, Stanislava and Glamočlija, Jasmina and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2010",
abstract = "In this study, we investigated splenic and lung cell responses to nonlethal systemic Aspergillus fumigatus infection in mice. Apart from basic indices of spleen and lung cell activity, IL-17 expression by cells from both tissues was determined and compared to the expression of IFN-gamma and IL-4. Progressive decrease in tissue fungal burden correlated with increased spleen and lung cell activity. Increased IL-17 message was noted in spleen cells at all time points (3, 7 and 15 days post-infection; p.i.), while a modest increase in IFN-gamma mRNA expression was noted at day 3 p.i. Increased cytokine production at days 3 and 7 (IL-17) and throughout the experimental period (IFN-gamma) was found. In contrast, spleen cell IL-4 expression was considerably lower during infection, resulting in high IFN-gamma/IL-4 and IL-17/IL-4 ratios in the spleen. Pro-inflammatory cytokine response was observed in the lungs as well, but primarily as the result of increased production of IFN-gamma by lung cells in response to challenge with conidia and the absence of change in IL-4 response. Increased activity of cells from both tissues, as well as the pattern of cytokine production, created an optimal pro-inflammatory milieu for fungal eradication.",
journal = "Medical Mycology",
title = "Splenic and lung response to nonlethal systemic Aspergillus fumigatus infection in C57BL/6 mice",
number = "5",
volume = "48",
pages = "743",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1359"
}

Mirkov, I., Stojanović, I. D., Stošić-Grujičić, S., Glamočlija, J., Zolotarevski, L. D., Kataranovski, D. S.,& Kataranovski, M.. (2010). Splenic and lung response to nonlethal systemic Aspergillus fumigatus infection in C57BL/6 mice. in Medical Mycology, 48(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1359

Mirkov I, Stojanović ID, Stošić-Grujičić S, Glamočlija J, Zolotarevski LD, Kataranovski DS, Kataranovski M. Splenic and lung response to nonlethal systemic Aspergillus fumigatus infection in C57BL/6 mice. in Medical Mycology. 2010;48(5):null-743.
https://hdl.handle.net/21.15107/rcub_ibiss_1359 .

Mirkov, Ivana, Stojanović, Ivana D., Stošić-Grujičić, Stanislava, Glamočlija, Jasmina, Zolotarevski, Lidija D, Kataranovski, Dragan S., Kataranovski, Milena, "Splenic and lung response to nonlethal systemic Aspergillus fumigatus infection in C57BL/6 mice" in Medical Mycology, 48, no. 5 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1359 .

TY  - JOUR
AU  - Kataranovski, Milena
AU  - Mirkov, Ivana
AU  - Belij, Sandra
AU  - Nikolić, Miroslav
AU  - Zolotarevski, Lidija D
AU  - Ćirić, Danica
AU  - Kataranovski, Dragan S.
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1433
AB  - Pulmonary inflammation is a biological response to cadmium entering the body via the respiratory route. Systemic administration of this metal revealed the lungs as a significant site of its disposition. In this study, the presence of basic indicators of lung inflammation (leukocyte infiltration and activity of cells recovered from lungs by enzyme digestion) was analyzed in the rat model of acute systemic cadmium intoxication. Intraperitoneal administration of both cadmium doses (0.5 mg/kg and 1.0 mg/kg) resulted in increased numbers of neutrophils. Signs of spontaneous activation of lung cells including the capacity of reduction of nitroblue tetrazolium (NBT), increase in myeloperoxidase (MPO) intracellular content and increase in interleukin-6 (IL-6) production were noted at both cadmium doses. Increased lung cell responsiveness to stimulation in vitro was noted at the higher cadmium dose. The presence of pulmonary inflammatory parameters in rats administered intraperitoneally with cadmium revealed the lungs as remote inflammatory targets of this metal. (C) 2009 Elsevier B.V. All rights reserved.
T2  - Environmental Toxicology and Pharmacology
T1  - Lungs: Remote inflammatory target of systemic cadmium administration in rats
IS  - 2
VL  - 28
EP  - 231
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1433
ER  - 

@article{
author = "Kataranovski, Milena and Mirkov, Ivana and Belij, Sandra and Nikolić, Miroslav and Zolotarevski, Lidija D and Ćirić, Danica and Kataranovski, Dragan S.",
year = "2009",
abstract = "Pulmonary inflammation is a biological response to cadmium entering the body via the respiratory route. Systemic administration of this metal revealed the lungs as a significant site of its disposition. In this study, the presence of basic indicators of lung inflammation (leukocyte infiltration and activity of cells recovered from lungs by enzyme digestion) was analyzed in the rat model of acute systemic cadmium intoxication. Intraperitoneal administration of both cadmium doses (0.5 mg/kg and 1.0 mg/kg) resulted in increased numbers of neutrophils. Signs of spontaneous activation of lung cells including the capacity of reduction of nitroblue tetrazolium (NBT), increase in myeloperoxidase (MPO) intracellular content and increase in interleukin-6 (IL-6) production were noted at both cadmium doses. Increased lung cell responsiveness to stimulation in vitro was noted at the higher cadmium dose. The presence of pulmonary inflammatory parameters in rats administered intraperitoneally with cadmium revealed the lungs as remote inflammatory targets of this metal. (C) 2009 Elsevier B.V. All rights reserved.",
journal = "Environmental Toxicology and Pharmacology",
title = "Lungs: Remote inflammatory target of systemic cadmium administration in rats",
number = "2",
volume = "28",
pages = "231",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1433"
}

Kataranovski, M., Mirkov, I., Belij, S., Nikolić, M., Zolotarevski, L. D., Ćirić, D.,& Kataranovski, D. S.. (2009). Lungs: Remote inflammatory target of systemic cadmium administration in rats. in Environmental Toxicology and Pharmacology, 28(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1433

Kataranovski M, Mirkov I, Belij S, Nikolić M, Zolotarevski LD, Ćirić D, Kataranovski DS. Lungs: Remote inflammatory target of systemic cadmium administration in rats. in Environmental Toxicology and Pharmacology. 2009;28(2):null-231.
https://hdl.handle.net/21.15107/rcub_ibiss_1433 .

Kataranovski, Milena, Mirkov, Ivana, Belij, Sandra, Nikolić, Miroslav, Zolotarevski, Lidija D, Ćirić, Danica, Kataranovski, Dragan S., "Lungs: Remote inflammatory target of systemic cadmium administration in rats" in Environmental Toxicology and Pharmacology, 28, no. 2 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1433 .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Zolotarevski, Lidija D
AU  - Glamočlija, Jasmina
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1527
AB  - Objective. - Systemic response to intravenous administration of Aspergillus fumigatus conidia was investigated in laboratory mice by survival/mortality, peripheral blood Leukocyte response and histopathological evaluation of Lung, liver, kidney and spleen. Methods. - Female C57Bl/6 mice were inoculated intravenously with 10(6) -5 x 10(7) conidia of A. fumigatus (human isolate). Survival was monitored for two weeks. Histopathological analysis of organ was conducted postmortem and in survivors. Peripheral blood cells and leukocyte aggregation were determined in survivors. Results. - Administration of all doses of A. fumigatus conidia caused mortality, but the highest mortality rate and the shortest time of survival were noted at the highest doses applied. Increased peripheral blood lymphocyte counts and their propensity to aggregate were observed in survivors. Histological analysis revealed : (1) pulmonary inflammatory response proportional to the applied dose of conidia with detectable fungi in lungs of animals that received the highest applied dose; (2) pronounced kidney inflammatory response with variable intensity of tubular dilation in survivors versus tubular destruction in nonsurvivors and proteinuria as well as hemoglobinuria at the highest doses applied; (3) presence of microabscesses in the liver; (4) white pulp hyperplasia and dose-dependent increase in lymphoid follicles in the spleen of infected animals that survived. Conclusion. - Lymphocytosis and state of aggregation/adhesion in survivors and local histotogically evident inflammatory response in all examined organs of mice characterize the response to systemic application of A. fumigatus conidia. The results obtained may contribute to our understanding of pathological mechanisms underlying disseminated aspergillosis and to the evaluation of therapeutic interventions that might improve survival or Lessen pathology. (C) 2008 Published by Elsevier Masson SAS.
T2  - Journal de Mycologie Medicale
T1  - Experimental disseminated aspergillosis in mice: Histopathological study
IS  - 2
VL  - 18
EP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1527
ER  - 

@article{
author = "Mirkov, Ivana and Zolotarevski, Lidija D and Glamočlija, Jasmina and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2008",
abstract = "Objective. - Systemic response to intravenous administration of Aspergillus fumigatus conidia was investigated in laboratory mice by survival/mortality, peripheral blood Leukocyte response and histopathological evaluation of Lung, liver, kidney and spleen. Methods. - Female C57Bl/6 mice were inoculated intravenously with 10(6) -5 x 10(7) conidia of A. fumigatus (human isolate). Survival was monitored for two weeks. Histopathological analysis of organ was conducted postmortem and in survivors. Peripheral blood cells and leukocyte aggregation were determined in survivors. Results. - Administration of all doses of A. fumigatus conidia caused mortality, but the highest mortality rate and the shortest time of survival were noted at the highest doses applied. Increased peripheral blood lymphocyte counts and their propensity to aggregate were observed in survivors. Histological analysis revealed : (1) pulmonary inflammatory response proportional to the applied dose of conidia with detectable fungi in lungs of animals that received the highest applied dose; (2) pronounced kidney inflammatory response with variable intensity of tubular dilation in survivors versus tubular destruction in nonsurvivors and proteinuria as well as hemoglobinuria at the highest doses applied; (3) presence of microabscesses in the liver; (4) white pulp hyperplasia and dose-dependent increase in lymphoid follicles in the spleen of infected animals that survived. Conclusion. - Lymphocytosis and state of aggregation/adhesion in survivors and local histotogically evident inflammatory response in all examined organs of mice characterize the response to systemic application of A. fumigatus conidia. The results obtained may contribute to our understanding of pathological mechanisms underlying disseminated aspergillosis and to the evaluation of therapeutic interventions that might improve survival or Lessen pathology. (C) 2008 Published by Elsevier Masson SAS.",
journal = "Journal de Mycologie Medicale",
title = "Experimental disseminated aspergillosis in mice: Histopathological study",
number = "2",
volume = "18",
pages = "82",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1527"
}

Mirkov, I., Zolotarevski, L. D., Glamočlija, J., Kataranovski, D. S.,& Kataranovski, M.. (2008). Experimental disseminated aspergillosis in mice: Histopathological study. in Journal de Mycologie Medicale, 18(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1527

Mirkov I, Zolotarevski LD, Glamočlija J, Kataranovski DS, Kataranovski M. Experimental disseminated aspergillosis in mice: Histopathological study. in Journal de Mycologie Medicale. 2008;18(2):null-82.
https://hdl.handle.net/21.15107/rcub_ibiss_1527 .

Mirkov, Ivana, Zolotarevski, Lidija D, Glamočlija, Jasmina, Kataranovski, Dragan S., Kataranovski, Milena, "Experimental disseminated aspergillosis in mice: Histopathological study" in Journal de Mycologie Medicale, 18, no. 2 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1527 .

TY  - JOUR
AU  - Kataranovski, Milena
AU  - Kataranovski, Dragan S.
AU  - Zolotarevski, Lidija D
AU  - Jović, Milena
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1615
AB  - Dermatotoxic effects of epicutaneous application of a first generation anticoagulant, warfarin (WF), were examined in rats. Selected parameters of skin activity were determined 24 hours following warfarin application by histomorphological and immunohistochemical analysis and by assessing some aspects of immunomodulatory potential of warfarin in skin. Increased number of mast cells, with degranulation at higher doses of warfarin was noted in warfarin treated skin. Mast cell presence coincided with changes in blood vessels and fibroblast appearance suggesting mast cell activity in warfarin treated skin. Signs of nuclear hypertrophy and anysonucleosys were noted by analysis of PCNA(+) cells in epidermis following warfarin application. Histomorphological changes were accompanied by immunemodulating activity in warfarin treated skin. This was judged by slightly increased numbers of CD3(+) cells in epidermis and superficial dermis and by production of organ cultured full thickness skin explants of factors with costimulatory activity in T-cell activation/proliferation assay. Presented data demonstrates the potential of warfarin to modulate local skin activity in rats.
T2  - Cutaneous and Ocular Toxicology
T1  - Epicutaneous exposure to anticoagulant rodenticide warfarin modulates local skin activity in rats
IS  - 1
VL  - 26
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1615
ER  - 

@article{
author = "Kataranovski, Milena and Kataranovski, Dragan S. and Zolotarevski, Lidija D and Jović, Milena",
year = "2007",
abstract = "Dermatotoxic effects of epicutaneous application of a first generation anticoagulant, warfarin (WF), were examined in rats. Selected parameters of skin activity were determined 24 hours following warfarin application by histomorphological and immunohistochemical analysis and by assessing some aspects of immunomodulatory potential of warfarin in skin. Increased number of mast cells, with degranulation at higher doses of warfarin was noted in warfarin treated skin. Mast cell presence coincided with changes in blood vessels and fibroblast appearance suggesting mast cell activity in warfarin treated skin. Signs of nuclear hypertrophy and anysonucleosys were noted by analysis of PCNA(+) cells in epidermis following warfarin application. Histomorphological changes were accompanied by immunemodulating activity in warfarin treated skin. This was judged by slightly increased numbers of CD3(+) cells in epidermis and superficial dermis and by production of organ cultured full thickness skin explants of factors with costimulatory activity in T-cell activation/proliferation assay. Presented data demonstrates the potential of warfarin to modulate local skin activity in rats.",
journal = "Cutaneous and Ocular Toxicology",
title = "Epicutaneous exposure to anticoagulant rodenticide warfarin modulates local skin activity in rats",
number = "1",
volume = "26",
pages = "13",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1615"
}

Kataranovski, M., Kataranovski, D. S., Zolotarevski, L. D.,& Jović, M.. (2007). Epicutaneous exposure to anticoagulant rodenticide warfarin modulates local skin activity in rats. in Cutaneous and Ocular Toxicology, 26(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1615

Kataranovski M, Kataranovski DS, Zolotarevski LD, Jović M. Epicutaneous exposure to anticoagulant rodenticide warfarin modulates local skin activity in rats. in Cutaneous and Ocular Toxicology. 2007;26(1):null-13.
https://hdl.handle.net/21.15107/rcub_ibiss_1615 .

Kataranovski, Milena, Kataranovski, Dragan S., Zolotarevski, Lidija D, Jović, Milena, "Epicutaneous exposure to anticoagulant rodenticide warfarin modulates local skin activity in rats" in Cutaneous and Ocular Toxicology, 26, no. 1 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1615 .

TY  - JOUR
AU  - Kataranovski, Milena
AU  - Prokić, Vera
AU  - Kataranovski, Dragan S.
AU  - Zolotarevski, Lidija D
AU  - Majstorović, Ivana J
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1692
AB  - Dermatotoxic effects of epicutaneous application of a first-generation anticoagulant, warfarin (WF) were examined in rats. Selected parameters of skin activity were determined 24 h following warfarin application, including metabolic viability of skin explants, some aspects of oxidative activity in skin tissue homogenates and inflammatory/immune relevant activity of epidermal cells from warfarin-treated skin. No changes in skin metabolic viability (MTT reduction) were noted ex vivo following WF application, suggesting the absence of immediate toxicity for skin. In contrast, increased formation of malondialdehyde (MDA), with a decrease in protein and non-protein thiols in homogenates of warfarin-treated skin was demonstrated, pointing to prooxidant activity in warfarin-treated skin. Increased costimulatory activity of epidermal cells isolated from warfarin-exposed skin in ConA-stimulated T-cell activation/proliferation assay was noted, reflecting proinflammatory and immune-modulating capacity of warfarin for epidermis. No evident differences in skin histology between control and warfarin-treated skin were found at that time point, while striking changes in tissue integrity, cellularity and appearance 72h following WF application were noted. The observed histological picture probably reflects a regenerative/inflammatory program related to oxidant/inflammation-type warfarin-evoked injury to the skin. Presented data demonstrate the potential of epicutaneously applied warfarin to modulate local skin activity in rats. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
T2  - Toxicology
T1  - Dermatotoxicity of epicutaneously applied anticoagulant warfarin
IS  - 2-3
VL  - 212
EP  - 218
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1692
ER  - 

@article{
author = "Kataranovski, Milena and Prokić, Vera and Kataranovski, Dragan S. and Zolotarevski, Lidija D and Majstorović, Ivana J",
year = "2005",
abstract = "Dermatotoxic effects of epicutaneous application of a first-generation anticoagulant, warfarin (WF) were examined in rats. Selected parameters of skin activity were determined 24 h following warfarin application, including metabolic viability of skin explants, some aspects of oxidative activity in skin tissue homogenates and inflammatory/immune relevant activity of epidermal cells from warfarin-treated skin. No changes in skin metabolic viability (MTT reduction) were noted ex vivo following WF application, suggesting the absence of immediate toxicity for skin. In contrast, increased formation of malondialdehyde (MDA), with a decrease in protein and non-protein thiols in homogenates of warfarin-treated skin was demonstrated, pointing to prooxidant activity in warfarin-treated skin. Increased costimulatory activity of epidermal cells isolated from warfarin-exposed skin in ConA-stimulated T-cell activation/proliferation assay was noted, reflecting proinflammatory and immune-modulating capacity of warfarin for epidermis. No evident differences in skin histology between control and warfarin-treated skin were found at that time point, while striking changes in tissue integrity, cellularity and appearance 72h following WF application were noted. The observed histological picture probably reflects a regenerative/inflammatory program related to oxidant/inflammation-type warfarin-evoked injury to the skin. Presented data demonstrate the potential of epicutaneously applied warfarin to modulate local skin activity in rats. (c) 2005 Elsevier Ireland Ltd. All rights reserved.",
journal = "Toxicology",
title = "Dermatotoxicity of epicutaneously applied anticoagulant warfarin",
number = "2-3",
volume = "212",
pages = "218",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1692"
}

Kataranovski, M., Prokić, V., Kataranovski, D. S., Zolotarevski, L. D.,& Majstorović, I. J.. (2005). Dermatotoxicity of epicutaneously applied anticoagulant warfarin. in Toxicology, 212(2-3).
https://hdl.handle.net/21.15107/rcub_ibiss_1692

Kataranovski M, Prokić V, Kataranovski DS, Zolotarevski LD, Majstorović IJ. Dermatotoxicity of epicutaneously applied anticoagulant warfarin. in Toxicology. 2005;212(2-3):null-218.
https://hdl.handle.net/21.15107/rcub_ibiss_1692 .

Kataranovski, Milena, Prokić, Vera, Kataranovski, Dragan S., Zolotarevski, Lidija D, Majstorović, Ivana J, "Dermatotoxicity of epicutaneously applied anticoagulant warfarin" in Toxicology, 212, no. 2-3 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1692 .