TY  - JOUR
AU  - Tsasi, Gerasimia
AU  - Danalatos, Gerasimos
AU  - Tomou, Ekaterina-Michaela
AU  - Sakadani, Eleni
AU  - Bethanis, Mihalis
AU  - Kolokouris, Aristides
AU  - Samaras, Yiannis
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Skaltsa, Helen
PY  - 2022
UR  - https://www.tandfonline.com/doi/full/10.1080/10412905.2022.2032421
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4791
AB  - Abies cephalonica Loudon consists one of the three fir species occurring in Greece. The aim of this study was to evaluate the chemical composition of the essential oil (EO) from needles of A. cephalonica from Mt. Aenos (Kefalonia Island, Greece) and to investigate its antimicrobial activity. The EO was obtained by hydrodistillation and 68 constituents corresponding to 99.4% of the EO were identified by using GC-MS analysis. The major chemical category of the volatile compounds were the monoterpene hydrocarbons (63.9%), followed by oxygenated monoterpenes (20.2%). In particular, the main components were beta-pinene (26.9%), bornyl acetate (12.5%), alpha-pinene (10.1%), camphene (9.2%), limonene (8.1%), gamma-eudesmol (8.1%) and beta-phellandrene (5.8%). To the best of our knowledge, the present study is the first research, reporting the phytochemical profile and the antimicrobial potential of the EO of A. cephalonica from this distinct geographic area.
T2  - Journal of Essential Oil Research
T1  - Chemical composition and antimicrobial activity of the essential oil of Abies cephalonica Loudon from Mount Ainos (Kefalonia, Greece)
IS  - 2
VL  - 34
DO  - 10.1080/10412905.2022.2032421
SP  - 143
EP  - 147
ER  - 

@article{
author = "Tsasi, Gerasimia and Danalatos, Gerasimos and Tomou, Ekaterina-Michaela and Sakadani, Eleni and Bethanis, Mihalis and Kolokouris, Aristides and Samaras, Yiannis and Ćirić, Ana and Soković, Marina and Skaltsa, Helen",
year = "2022",
abstract = "Abies cephalonica Loudon consists one of the three fir species occurring in Greece. The aim of this study was to evaluate the chemical composition of the essential oil (EO) from needles of A. cephalonica from Mt. Aenos (Kefalonia Island, Greece) and to investigate its antimicrobial activity. The EO was obtained by hydrodistillation and 68 constituents corresponding to 99.4% of the EO were identified by using GC-MS analysis. The major chemical category of the volatile compounds were the monoterpene hydrocarbons (63.9%), followed by oxygenated monoterpenes (20.2%). In particular, the main components were beta-pinene (26.9%), bornyl acetate (12.5%), alpha-pinene (10.1%), camphene (9.2%), limonene (8.1%), gamma-eudesmol (8.1%) and beta-phellandrene (5.8%). To the best of our knowledge, the present study is the first research, reporting the phytochemical profile and the antimicrobial potential of the EO of A. cephalonica from this distinct geographic area.",
journal = "Journal of Essential Oil Research",
title = "Chemical composition and antimicrobial activity of the essential oil of Abies cephalonica Loudon from Mount Ainos (Kefalonia, Greece)",
number = "2",
volume = "34",
doi = "10.1080/10412905.2022.2032421",
pages = "143-147"
}

Tsasi, G., Danalatos, G., Tomou, E., Sakadani, E., Bethanis, M., Kolokouris, A., Samaras, Y., Ćirić, A., Soković, M.,& Skaltsa, H.. (2022). Chemical composition and antimicrobial activity of the essential oil of Abies cephalonica Loudon from Mount Ainos (Kefalonia, Greece). in Journal of Essential Oil Research, 34(2), 143-147.
https://doi.org/10.1080/10412905.2022.2032421

Tsasi G, Danalatos G, Tomou E, Sakadani E, Bethanis M, Kolokouris A, Samaras Y, Ćirić A, Soković M, Skaltsa H. Chemical composition and antimicrobial activity of the essential oil of Abies cephalonica Loudon from Mount Ainos (Kefalonia, Greece). in Journal of Essential Oil Research. 2022;34(2):143-147.
doi:10.1080/10412905.2022.2032421 .

Tsasi, Gerasimia, Danalatos, Gerasimos, Tomou, Ekaterina-Michaela, Sakadani, Eleni, Bethanis, Mihalis, Kolokouris, Aristides, Samaras, Yiannis, Ćirić, Ana, Soković, Marina, Skaltsa, Helen, "Chemical composition and antimicrobial activity of the essential oil of Abies cephalonica Loudon from Mount Ainos (Kefalonia, Greece)" in Journal of Essential Oil Research, 34, no. 2 (2022):143-147,
https://doi.org/10.1080/10412905.2022.2032421 . .

TY  - JOUR
AU  - Vanti, Giulia
AU  - Tomou, Ekaterina Michaela
AU  - Stojković, Dejan
AU  - Ćirić, Ana
AU  - Bilia, Anna Rita
AU  - Skaltsa, Helen
PY  - 2021
UR  - https://doi.org/10.3390/molecules26082124
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4237
AB  - Food poisoning is a common cause of illness and death in developing countries. Essential oils (EOs) could be effective and safe natural preservatives to prevent and control bacterial contamination of foods. However, their high sensitivity and strong flavor limit their application and biological effectiveness. The aim of this study was firstly the chemical analysis and the antimicrobial evaluation of the EOs of Origanum onites L. and Satureja thymbra L. obtained from Symi island (Greece), and, secondly, the formulation of propylene glycol-nanovesicles loaded with these EOs to improve their antimicrobial properties. The EOs were analyzed by GC-MS and their chemical contents are presented herein. Different nanovesicles were formulated with small average sizes, high homogeneity, and optimal ζ-potential. Microscopic observation confirmed their small and spherical shape. Antibacterial and antifungal activities of the formulated EOs were evaluated against food-borne pathogens and spoilage microorganisms compared to pure EOs. Propylene glycol-nanovesicles loaded with O. onites EO were found to be the most active formulation against all tested strains. Additionally, in vitro studies on the HaCaT cell line showed that nanovesicles encapsulated with EOs had no toxic effect. The present study revealed that both EOs can be used as alternative sanitizers and preservatives in the food industry, and that their formulation in nanovesicles can provide a suitable approach as food-grade delivery system.
PB  - MDPI AG
T2  - Molecules
T1  - Nanovesicles loaded with origanum onites and satureja thymbra essential oils and their activity against food-borne pathogens and spoilage microorganisms
IS  - 8
VL  - 26
DO  - 10.3390/molecules26082124
SP  - 2124
ER  - 

@article{
author = "Vanti, Giulia and Tomou, Ekaterina Michaela and Stojković, Dejan and Ćirić, Ana and Bilia, Anna Rita and Skaltsa, Helen",
year = "2021",
abstract = "Food poisoning is a common cause of illness and death in developing countries. Essential oils (EOs) could be effective and safe natural preservatives to prevent and control bacterial contamination of foods. However, their high sensitivity and strong flavor limit their application and biological effectiveness. The aim of this study was firstly the chemical analysis and the antimicrobial evaluation of the EOs of Origanum onites L. and Satureja thymbra L. obtained from Symi island (Greece), and, secondly, the formulation of propylene glycol-nanovesicles loaded with these EOs to improve their antimicrobial properties. The EOs were analyzed by GC-MS and their chemical contents are presented herein. Different nanovesicles were formulated with small average sizes, high homogeneity, and optimal ζ-potential. Microscopic observation confirmed their small and spherical shape. Antibacterial and antifungal activities of the formulated EOs were evaluated against food-borne pathogens and spoilage microorganisms compared to pure EOs. Propylene glycol-nanovesicles loaded with O. onites EO were found to be the most active formulation against all tested strains. Additionally, in vitro studies on the HaCaT cell line showed that nanovesicles encapsulated with EOs had no toxic effect. The present study revealed that both EOs can be used as alternative sanitizers and preservatives in the food industry, and that their formulation in nanovesicles can provide a suitable approach as food-grade delivery system.",
publisher = "MDPI AG",
journal = "Molecules",
title = "Nanovesicles loaded with origanum onites and satureja thymbra essential oils and their activity against food-borne pathogens and spoilage microorganisms",
number = "8",
volume = "26",
doi = "10.3390/molecules26082124",
pages = "2124"
}

Vanti, G., Tomou, E. M., Stojković, D., Ćirić, A., Bilia, A. R.,& Skaltsa, H.. (2021). Nanovesicles loaded with origanum onites and satureja thymbra essential oils and their activity against food-borne pathogens and spoilage microorganisms. in Molecules
MDPI AG., 26(8), 2124.
https://doi.org/10.3390/molecules26082124

Vanti G, Tomou EM, Stojković D, Ćirić A, Bilia AR, Skaltsa H. Nanovesicles loaded with origanum onites and satureja thymbra essential oils and their activity against food-borne pathogens and spoilage microorganisms. in Molecules. 2021;26(8):2124.
doi:10.3390/molecules26082124 .

Vanti, Giulia, Tomou, Ekaterina Michaela, Stojković, Dejan, Ćirić, Ana, Bilia, Anna Rita, Skaltsa, Helen, "Nanovesicles loaded with origanum onites and satureja thymbra essential oils and their activity against food-borne pathogens and spoilage microorganisms" in Molecules, 26, no. 8 (2021):2124,
https://doi.org/10.3390/molecules26082124 . .

TY  - JOUR
AU  - Grafakou, Maria Eleni
AU  - Diamanti, Aggeliki
AU  - Antaloudaki, Eleftheria
AU  - Kypriotakis, Zacharias
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Skaltsa, Helen
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3678
AB  - The volatile compositions of three closely related Hypericum species growing wild on the island of Crete were studied, all belonging to the section Coridium. Hydro-distillation in a modified Clevenger-type apparatus was performed according to the Hellenic Pharmacopoeia in order to obtain the essential oils, which were analyzed by GC-MS. Identification of the compounds was carried out by comparison of MS spectra and retention indices with literature data, as well as by co-chromatography with authentic samples. In total, 123 different compounds were identified and the main compounds were by order of their abundance as follows: H. empetrifolium: α-pinene, germacrene D, β-pinene, E-caryophyllene; H. amblycalyx: β-elemene, β-selinene, α-pinene, E-caryophyllene, α-selinene; H. jovis: trans-calamenene, α-selinene, β-elemene. The chemical results revealed the differences and similarities (qualitative and quantitative) between the studied oils, supporting the hypothesis that essential oils from Hypericum spp. do not serve as chemotaxonomic markers. Moreover, the essential oils were subjected to antimicrobial screening. According to the given results, the essential oils possessed better antifungal and anticandidal activities than antibacterial activities.
PB  - MDPI AG
T2  - Applied Sciences (Switzerland)
T1  - Chemical composition and antimicrobial activity of the essential oils of three closely related hypericum species growing wild on the island of Crete, Greece
IS  - 8
VL  - 10
DO  - 10.3390/APP10082823
SP  - 2823
ER  - 

@article{
author = "Grafakou, Maria Eleni and Diamanti, Aggeliki and Antaloudaki, Eleftheria and Kypriotakis, Zacharias and Ćirić, Ana and Soković, Marina and Skaltsa, Helen",
year = "2020",
abstract = "The volatile compositions of three closely related Hypericum species growing wild on the island of Crete were studied, all belonging to the section Coridium. Hydro-distillation in a modified Clevenger-type apparatus was performed according to the Hellenic Pharmacopoeia in order to obtain the essential oils, which were analyzed by GC-MS. Identification of the compounds was carried out by comparison of MS spectra and retention indices with literature data, as well as by co-chromatography with authentic samples. In total, 123 different compounds were identified and the main compounds were by order of their abundance as follows: H. empetrifolium: α-pinene, germacrene D, β-pinene, E-caryophyllene; H. amblycalyx: β-elemene, β-selinene, α-pinene, E-caryophyllene, α-selinene; H. jovis: trans-calamenene, α-selinene, β-elemene. The chemical results revealed the differences and similarities (qualitative and quantitative) between the studied oils, supporting the hypothesis that essential oils from Hypericum spp. do not serve as chemotaxonomic markers. Moreover, the essential oils were subjected to antimicrobial screening. According to the given results, the essential oils possessed better antifungal and anticandidal activities than antibacterial activities.",
publisher = "MDPI AG",
journal = "Applied Sciences (Switzerland)",
title = "Chemical composition and antimicrobial activity of the essential oils of three closely related hypericum species growing wild on the island of Crete, Greece",
number = "8",
volume = "10",
doi = "10.3390/APP10082823",
pages = "2823"
}

Grafakou, M. E., Diamanti, A., Antaloudaki, E., Kypriotakis, Z., Ćirić, A., Soković, M.,& Skaltsa, H.. (2020). Chemical composition and antimicrobial activity of the essential oils of three closely related hypericum species growing wild on the island of Crete, Greece. in Applied Sciences (Switzerland)
MDPI AG., 10(8), 2823.
https://doi.org/10.3390/APP10082823

Grafakou ME, Diamanti A, Antaloudaki E, Kypriotakis Z, Ćirić A, Soković M, Skaltsa H. Chemical composition and antimicrobial activity of the essential oils of three closely related hypericum species growing wild on the island of Crete, Greece. in Applied Sciences (Switzerland). 2020;10(8):2823.
doi:10.3390/APP10082823 .

Grafakou, Maria Eleni, Diamanti, Aggeliki, Antaloudaki, Eleftheria, Kypriotakis, Zacharias, Ćirić, Ana, Soković, Marina, Skaltsa, Helen, "Chemical composition and antimicrobial activity of the essential oils of three closely related hypericum species growing wild on the island of Crete, Greece" in Applied Sciences (Switzerland), 10, no. 8 (2020):2823,
https://doi.org/10.3390/APP10082823 . .

TY  - JOUR
AU  - Soković, Marina
AU  - Skaltsa, Helen
AU  - Ferreira, Isabel C. F. R.
PY  - 2019
UR  - https://www.frontiersin.org/article/10.3389/fpls.2019.01464/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6868025
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3563
PB  - Frontiers
T2  - Frontiers in Plant Science
T1  - Editorial: Bioactive Phytochemicals in Asteraceae: Structure, Function, and Biological Activity.
VL  - 10
DO  - 10.3389/fpls.2019.01464
SP  - 1464
ER  - 

@article{
author = "Soković, Marina and Skaltsa, Helen and Ferreira, Isabel C. F. R.",
year = "2019",
publisher = "Frontiers",
journal = "Frontiers in Plant Science",
title = "Editorial: Bioactive Phytochemicals in Asteraceae: Structure, Function, and Biological Activity.",
volume = "10",
doi = "10.3389/fpls.2019.01464",
pages = "1464"
}

Soković, M., Skaltsa, H.,& Ferreira, I. C. F. R.. (2019). Editorial: Bioactive Phytochemicals in Asteraceae: Structure, Function, and Biological Activity.. in Frontiers in Plant Science
Frontiers., 10, 1464.
https://doi.org/10.3389/fpls.2019.01464

Soković M, Skaltsa H, Ferreira ICFR. Editorial: Bioactive Phytochemicals in Asteraceae: Structure, Function, and Biological Activity.. in Frontiers in Plant Science. 2019;10:1464.
doi:10.3389/fpls.2019.01464 .

Soković, Marina, Skaltsa, Helen, Ferreira, Isabel C. F. R., "Editorial: Bioactive Phytochemicals in Asteraceae: Structure, Function, and Biological Activity." in Frontiers in Plant Science, 10 (2019):1464,
https://doi.org/10.3389/fpls.2019.01464 . .

TY  - JOUR
AU  - Barda, Christina
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Tsoukalas, Michael
AU  - Skaltsa, Helen
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0305197818302345?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3110
AB  - The genus Crepis L. belongs to the Cichorieae tribe of the Asteraceae family. C. incana Sm. is a narrow endemic species of southern Greece. Crepis L. is well known for its abundance in sesquiterpene lactones. In total, from its aerial parts nine compounds were isolated for the first time, i.e. three guaianolides, grosheimin, crepiside E, crepiside D; two germacranolides, taraxinic acid and its 1′-O-β-d-glucopyranosyl ester, the nor-isoprenoid (3S, 5R)-loliolide and three flavonoids, luteolin, luteolin 7-O-β-d-glucopyranoside and quercetin 3-O-β-d-glucopyranoside. Furthermore, the root extract afforded two triterpenes, oleanolic acid and lupeol. The structures of the isolated compounds were elucidated by high-field NMR spectroscopy.
T2  - Biochemical Systematics and Ecology
T1  - Phytochemical investigation of Crepis incana Sm. (Asteraceae) endemic to southern Greece
VL  - 80
DO  - 10.1016/J.BSE.2018.06.009
SP  - 59
EP  - 62
ER  - 

@article{
author = "Barda, Christina and Ćirić, Ana and Soković, Marina and Tsoukalas, Michael and Skaltsa, Helen",
year = "2018",
abstract = "The genus Crepis L. belongs to the Cichorieae tribe of the Asteraceae family. C. incana Sm. is a narrow endemic species of southern Greece. Crepis L. is well known for its abundance in sesquiterpene lactones. In total, from its aerial parts nine compounds were isolated for the first time, i.e. three guaianolides, grosheimin, crepiside E, crepiside D; two germacranolides, taraxinic acid and its 1′-O-β-d-glucopyranosyl ester, the nor-isoprenoid (3S, 5R)-loliolide and three flavonoids, luteolin, luteolin 7-O-β-d-glucopyranoside and quercetin 3-O-β-d-glucopyranoside. Furthermore, the root extract afforded two triterpenes, oleanolic acid and lupeol. The structures of the isolated compounds were elucidated by high-field NMR spectroscopy.",
journal = "Biochemical Systematics and Ecology",
title = "Phytochemical investigation of Crepis incana Sm. (Asteraceae) endemic to southern Greece",
volume = "80",
doi = "10.1016/J.BSE.2018.06.009",
pages = "59-62"
}

Barda, C., Ćirić, A., Soković, M., Tsoukalas, M.,& Skaltsa, H.. (2018). Phytochemical investigation of Crepis incana Sm. (Asteraceae) endemic to southern Greece. in Biochemical Systematics and Ecology, 80, 59-62.
https://doi.org/10.1016/J.BSE.2018.06.009

Barda C, Ćirić A, Soković M, Tsoukalas M, Skaltsa H. Phytochemical investigation of Crepis incana Sm. (Asteraceae) endemic to southern Greece. in Biochemical Systematics and Ecology. 2018;80:59-62.
doi:10.1016/J.BSE.2018.06.009 .

Barda, Christina, Ćirić, Ana, Soković, Marina, Tsoukalas, Michael, Skaltsa, Helen, "Phytochemical investigation of Crepis incana Sm. (Asteraceae) endemic to southern Greece" in Biochemical Systematics and Ecology, 80 (2018):59-62,
https://doi.org/10.1016/J.BSE.2018.06.009 . .

TY  - JOUR
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Skaltsa, Helen
PY  - 2017
UR  - http://www.eurekaselect.com/150118/article
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2835
AB  - Background: In recent years, a growing interest has developed in the field of biological activity of plant metabolites. Research in this area considering antimicrobial, antioxidant, cytotoxic, anti-inflammatory, and other properties, is currently expanding, reporting various species to possess such biological effects. Among them, Centaurea species are well known to be used in ethnomedicine. The Centaurea genus (Asteraceae) is represented by more than 500 species, mostly located in the Mediterranean region and Western Asia. Methods: The current revision focuses on up-to-date literature on the isolation, structural elucidation and biological properties of sesquiterepene lactones from Centaurea species, regarding antimicrobial, antitumour, antiviral, anti-inflammatory and cytotoxic properties. The methods for extraction, isolation and identification are also pointed out and the bioactivity of lactones as purified compounds discussed. Several general ideas about the structural requirements for activity, based on the in vitro bioassays and the in silico prediction of their ADME properties are discussed. Results: Investigations of chemical analyses and biological potential exhibited that flavonoids and sesquiterpene lactones, in particular germacranolides, eudesmanolides, elemanolides, and guaianolides, are compounds responsible for the biological potential of plants. Sesquiterpene lactones represent a group of compounds responsible for the medicinal characteristics of Centaurea species. Discussion: Investigation of structure-activity relationships of all data helps the understanding of the bioactivity resulting from chemical aspects. All activities are discussed profoundly, paying special attention to molecular mechanisms involved in the implication of the health-beneficial effects of these compounds and in order to point them out as candidates for new drug discovery, and as safe natural agents.
T2  - Current Pharmaceutical Design
T1  - Biological Activities of Sesquiterpene Lactones Isolated from the Genus Centaurea L. (Asteraceae)
IS  - 19
VL  - 23
DO  - 10.2174/1381612823666170215113927
SP  - 2767
EP  - 2786
ER  - 

@article{
author = "Soković, Marina and Ćirić, Ana and Glamočlija, Jasmina and Skaltsa, Helen",
year = "2017",
abstract = "Background: In recent years, a growing interest has developed in the field of biological activity of plant metabolites. Research in this area considering antimicrobial, antioxidant, cytotoxic, anti-inflammatory, and other properties, is currently expanding, reporting various species to possess such biological effects. Among them, Centaurea species are well known to be used in ethnomedicine. The Centaurea genus (Asteraceae) is represented by more than 500 species, mostly located in the Mediterranean region and Western Asia. Methods: The current revision focuses on up-to-date literature on the isolation, structural elucidation and biological properties of sesquiterepene lactones from Centaurea species, regarding antimicrobial, antitumour, antiviral, anti-inflammatory and cytotoxic properties. The methods for extraction, isolation and identification are also pointed out and the bioactivity of lactones as purified compounds discussed. Several general ideas about the structural requirements for activity, based on the in vitro bioassays and the in silico prediction of their ADME properties are discussed. Results: Investigations of chemical analyses and biological potential exhibited that flavonoids and sesquiterpene lactones, in particular germacranolides, eudesmanolides, elemanolides, and guaianolides, are compounds responsible for the biological potential of plants. Sesquiterpene lactones represent a group of compounds responsible for the medicinal characteristics of Centaurea species. Discussion: Investigation of structure-activity relationships of all data helps the understanding of the bioactivity resulting from chemical aspects. All activities are discussed profoundly, paying special attention to molecular mechanisms involved in the implication of the health-beneficial effects of these compounds and in order to point them out as candidates for new drug discovery, and as safe natural agents.",
journal = "Current Pharmaceutical Design",
title = "Biological Activities of Sesquiterpene Lactones Isolated from the Genus Centaurea L. (Asteraceae)",
number = "19",
volume = "23",
doi = "10.2174/1381612823666170215113927",
pages = "2767-2786"
}

Soković, M., Ćirić, A., Glamočlija, J.,& Skaltsa, H.. (2017). Biological Activities of Sesquiterpene Lactones Isolated from the Genus Centaurea L. (Asteraceae). in Current Pharmaceutical Design, 23(19), 2767-2786.
https://doi.org/10.2174/1381612823666170215113927

Soković M, Ćirić A, Glamočlija J, Skaltsa H. Biological Activities of Sesquiterpene Lactones Isolated from the Genus Centaurea L. (Asteraceae). in Current Pharmaceutical Design. 2017;23(19):2767-2786.
doi:10.2174/1381612823666170215113927 .

Soković, Marina, Ćirić, Ana, Glamočlija, Jasmina, Skaltsa, Helen, "Biological Activities of Sesquiterpene Lactones Isolated from the Genus Centaurea L. (Asteraceae)" in Current Pharmaceutical Design, 23, no. 19 (2017):2767-2786,
https://doi.org/10.2174/1381612823666170215113927 . .

TY  - JOUR
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Karioti, Anastasia
AU  - Skaltsa, Helen
PY  - 2011
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/563
AB  - Two coumarins, scopoletin (1) and isoscopoletin (2), two simple phenolic acids, protocatechuic acid (3) and isovanillic acid (4) and one flavonoid, eriodictyol (5) were isolated from the aerial parts of Centaurea spruneri. The structure of the compounds was established by spectroscopic methods. The in vitro antimicrobial activity of the isolated compounds was tested against eight bacteria and eight fungal species, using a microdilution method. All compounds tested showed moderate antibacterial and antifungal activities. Their minimum inhibitory concentrations were in the range 0.655-2.38 µmol ml-1 and their minimal bactericidal concentrations ranged from 0.694 to 4.15 µmol ml-1 against the tested bacterial species. All compounds showed fungistatic activity at 0.259-2.38 µmol ml-1 and fungicidal at 0.69-2.6 µmol ml-1 against all fungi tested.
AB  - Iz nadzemnog dela Centaurea spruneri izolovana su dva kumarina skopoletin (1) i izoskopoletin (2), dve proste fenolske kiseline protokatehuinska kiselina (3) i izovanilinska kiselina (4) i flavonoid eriodiktiol. Hemijske strukture poznatih jedinjenja su utvrđene korišćenjem 1D i 2D NMR, MS i UV spektroskopskih analiza. U in vitro testu za određivanje antimikrobne aktivnosti izolovanih jedinjenja korišćena je mikrodiluciona metoda. Dobijena jedinjenja testirana su na po osam bakterijskih i gljivičnih vrsta. Sva jedinjenja su imala umerenu antibakterijsku i antifungalnu aktivnost. Minimalna inhibitorna koncentracija testiranih jedinjenja se kretala od 0,655 do 2,38 µmol ml-1, a minimalna baktericidna koncentracija 0,694-4,15 µmol ml-1. Testirana jedinjenja su pokazala fungicidnu aktivnost od 0,259-2,38 µmol ml-1 i fungistatičku 0,694-2,60 µmol ml-1, na sve testirane gljive.
T2  - Journal of the Serbian Chemical Society
T1  - Antimikrobna aktivnost sekundarnih metabolita izolovanih iz Centaurea spruneri Boiss & Heldr.
T1  - Antimicrobial activity of secondary metabolites isolated from Centaurea spruneri Boiss. & Heldr.
IS  - 1
VL  - 76
SP  - 27
EP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_563
ER  - 

@article{
author = "Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina and Karioti, Anastasia and Skaltsa, Helen",
year = "2011, 2011",
abstract = "Two coumarins, scopoletin (1) and isoscopoletin (2), two simple phenolic acids, protocatechuic acid (3) and isovanillic acid (4) and one flavonoid, eriodictyol (5) were isolated from the aerial parts of Centaurea spruneri. The structure of the compounds was established by spectroscopic methods. The in vitro antimicrobial activity of the isolated compounds was tested against eight bacteria and eight fungal species, using a microdilution method. All compounds tested showed moderate antibacterial and antifungal activities. Their minimum inhibitory concentrations were in the range 0.655-2.38 µmol ml-1 and their minimal bactericidal concentrations ranged from 0.694 to 4.15 µmol ml-1 against the tested bacterial species. All compounds showed fungistatic activity at 0.259-2.38 µmol ml-1 and fungicidal at 0.69-2.6 µmol ml-1 against all fungi tested., Iz nadzemnog dela Centaurea spruneri izolovana su dva kumarina skopoletin (1) i izoskopoletin (2), dve proste fenolske kiseline protokatehuinska kiselina (3) i izovanilinska kiselina (4) i flavonoid eriodiktiol. Hemijske strukture poznatih jedinjenja su utvrđene korišćenjem 1D i 2D NMR, MS i UV spektroskopskih analiza. U in vitro testu za određivanje antimikrobne aktivnosti izolovanih jedinjenja korišćena je mikrodiluciona metoda. Dobijena jedinjenja testirana su na po osam bakterijskih i gljivičnih vrsta. Sva jedinjenja su imala umerenu antibakterijsku i antifungalnu aktivnost. Minimalna inhibitorna koncentracija testiranih jedinjenja se kretala od 0,655 do 2,38 µmol ml-1, a minimalna baktericidna koncentracija 0,694-4,15 µmol ml-1. Testirana jedinjenja su pokazala fungicidnu aktivnost od 0,259-2,38 µmol ml-1 i fungistatičku 0,694-2,60 µmol ml-1, na sve testirane gljive.",
journal = "Journal of the Serbian Chemical Society",
title = "Antimikrobna aktivnost sekundarnih metabolita izolovanih iz Centaurea spruneri Boiss & Heldr., Antimicrobial activity of secondary metabolites isolated from Centaurea spruneri Boiss. & Heldr.",
number = "1",
volume = "76",
pages = "27-34",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_563"
}

Ćirić, A., Glamočlija, J., Soković, M., Karioti, A.,& Skaltsa, H.. (2011). Antimikrobna aktivnost sekundarnih metabolita izolovanih iz Centaurea spruneri Boiss & Heldr.. in Journal of the Serbian Chemical Society, 76(1), 27-34.
https://hdl.handle.net/21.15107/rcub_ibiss_563

Ćirić A, Glamočlija J, Soković M, Karioti A, Skaltsa H. Antimikrobna aktivnost sekundarnih metabolita izolovanih iz Centaurea spruneri Boiss & Heldr.. in Journal of the Serbian Chemical Society. 2011;76(1):27-34.
https://hdl.handle.net/21.15107/rcub_ibiss_563 .

Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, Karioti, Anastasia, Skaltsa, Helen, "Antimikrobna aktivnost sekundarnih metabolita izolovanih iz Centaurea spruneri Boiss & Heldr." in Journal of the Serbian Chemical Society, 76, no. 1 (2011):27-34,
https://hdl.handle.net/21.15107/rcub_ibiss_563 .