TY  - CONF
AU  - Smailagić, Dijana
AU  - Dragišić Maksimović, Jelena
AU  - Marin, Marija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stupar, Sofija
AU  - Stanišić, Mariana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6710
AB  - Phloretin is a phenolic compound from the apple that belongs to the group of dihydrochalcones. The genus Malus is a uniquely rich source of dihydrochalcones, accounting for about 97% of the total phenolic compounds in apple leaves and 10-20% of the dry weight of the leaf tissue. Due to its typical dihydrochalcone structure, phloretin is a highly reactive molecule that interacts efficiently with many biological macromolecules. Its reactivity leads to remarkable antioxidant and medicinal properties that have been extensively studied in mammals and humans. However, knowledge about the effects of phloretin on plant organisms is rather scarce. The phytotoxicity of phloretin in plants was first documented by our research group when phloretin was recognised as a potent allelochemical of the apple tree with deleterious effects in plant species outside the genus Malus. Research on the model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh] named the disruption of auxin homeostasis in the roots as the main mechanism of the phytotoxic effect of phloretin. The present study complements previous research with new insights into the ultrastructural changes in leaf mesophyll cells that underlie the growth arrest and decay of the aerial parts of Arabidopsis seedlings exposed to phloretin. The effects of phloretin are closely linked to the ultrastructural damage in the leaf mesophyll cells and range from mild effects with short-term treatment to severe effects with long-term treatment. Transmission electron microscopy revealed changes in the morphology of the cell wall, changes in thylakoid organisation, swelling and displacement of chloroplasts after short-term treatment. A significant decrease in the number of starch granules and chlorophyll content in the leaves reflected impaired chloroplast function leading to depletion of starch reserves, starvation of cells and recruitment of micro- and macroautophagic processes for self-maintenance leading to programmed cell death after long-term treatment with phloretin. These results confirm that the sites of action of phloretin in plant and animal cells do not coincide, confirming the safety of its potential application as a new bioherbicidal agent for humans and animals.
PB  - Turkey. Gebze,Turkey: Technical University
C3  - Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey
T1  - How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?
SP  - ID-739
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6710
ER  - 

@conference{
author = "Smailagić, Dijana and Dragišić Maksimović, Jelena and Marin, Marija and Banjac, Nevena and Ninković, Slavica and Stupar, Sofija and Stanišić, Mariana",
year = "2024",
abstract = "Phloretin is a phenolic compound from the apple that belongs to the group of dihydrochalcones. The genus Malus is a uniquely rich source of dihydrochalcones, accounting for about 97% of the total phenolic compounds in apple leaves and 10-20% of the dry weight of the leaf tissue. Due to its typical dihydrochalcone structure, phloretin is a highly reactive molecule that interacts efficiently with many biological macromolecules. Its reactivity leads to remarkable antioxidant and medicinal properties that have been extensively studied in mammals and humans. However, knowledge about the effects of phloretin on plant organisms is rather scarce. The phytotoxicity of phloretin in plants was first documented by our research group when phloretin was recognised as a potent allelochemical of the apple tree with deleterious effects in plant species outside the genus Malus. Research on the model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh] named the disruption of auxin homeostasis in the roots as the main mechanism of the phytotoxic effect of phloretin. The present study complements previous research with new insights into the ultrastructural changes in leaf mesophyll cells that underlie the growth arrest and decay of the aerial parts of Arabidopsis seedlings exposed to phloretin. The effects of phloretin are closely linked to the ultrastructural damage in the leaf mesophyll cells and range from mild effects with short-term treatment to severe effects with long-term treatment. Transmission electron microscopy revealed changes in the morphology of the cell wall, changes in thylakoid organisation, swelling and displacement of chloroplasts after short-term treatment. A significant decrease in the number of starch granules and chlorophyll content in the leaves reflected impaired chloroplast function leading to depletion of starch reserves, starvation of cells and recruitment of micro- and macroautophagic processes for self-maintenance leading to programmed cell death after long-term treatment with phloretin. These results confirm that the sites of action of phloretin in plant and animal cells do not coincide, confirming the safety of its potential application as a new bioherbicidal agent for humans and animals.",
publisher = "Turkey. Gebze,Turkey: Technical University",
journal = "Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey",
title = "How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?",
pages = "ID-739",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6710"
}

Smailagić, D., Dragišić Maksimović, J., Marin, M., Banjac, N., Ninković, S., Stupar, S.,& Stanišić, M.. (2024). How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?. in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey
Turkey. Gebze,Turkey: Technical University., ID-739.
https://hdl.handle.net/21.15107/rcub_ibiss_6710

Smailagić D, Dragišić Maksimović J, Marin M, Banjac N, Ninković S, Stupar S, Stanišić M. How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?. in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey. 2024;:ID-739.
https://hdl.handle.net/21.15107/rcub_ibiss_6710 .

Smailagić, Dijana, Dragišić Maksimović, Jelena, Marin, Marija, Banjac, Nevena, Ninković, Slavica, Stupar, Sofija, Stanišić, Mariana, "How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?" in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey (2024):ID-739,
https://hdl.handle.net/21.15107/rcub_ibiss_6710 .

TY  - JOUR
AU  - Mašković, Pavle
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
AU  - Manojlović, Nedeljko T.
AU  - Radojković, Marija
AU  - Cvijović, Milica
AU  - Solujić, Slavica
PY  - 2012
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4675
AB  - The objective of this study was to evaluate the efficacy of the ethanolic extract of endemic plant Halacsya sendtneri in inhibiting the growing of the test fungi and bacteria as well as to determine its genotoxic potential and toxicity using the Allium anaphase-telophase assay. Minimum inhibitory concentrations (MIC) were determined for 15 indicator strains of pathogens, representing both bacteria and fungi. The highest susceptibility to the ethanolic extract of H. sendtneri was   exhibited by Pseudomonas glycinea (FSB4), (MIC=0.09 mg/ml) among the bacteria, and by Phialophora fastigiata (FSB81), (MIC=1.95 mg/ml) among the fungi. The composition of H. sendtneri extracts was also determined using HPLC analysis. Rosmarinic acid was found to be the dominant phenolic compound. The Allium anaphase-telophase genotoxicity assay revealed that the ethanolic extract of H. sendtneri at concentrations of 31.5 mg/l and below does not produce toxic or genotoxic effects. This is the first report of chemical constituents, genotoxic and antimicrobial activities of the endemic species, H.  sendtneri.
PB  - Warsaw: Versita
T2  - Central European Journal of Biology
T1  - Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler
IS  - 2
VL  - 7
DO  - 10.2478/s11535-012-0021-8
SP  - 327
EP  - 333
ER  - 

@article{
author = "Mašković, Pavle and Dragišić Maksimović, Jelena and Maksimović, Vuk and Blagojević, Jelena and Vujošević, Mladen and Manojlović, Nedeljko T. and Radojković, Marija and Cvijović, Milica and Solujić, Slavica",
year = "2012",
abstract = "The objective of this study was to evaluate the efficacy of the ethanolic extract of endemic plant Halacsya sendtneri in inhibiting the growing of the test fungi and bacteria as well as to determine its genotoxic potential and toxicity using the Allium anaphase-telophase assay. Minimum inhibitory concentrations (MIC) were determined for 15 indicator strains of pathogens, representing both bacteria and fungi. The highest susceptibility to the ethanolic extract of H. sendtneri was   exhibited by Pseudomonas glycinea (FSB4), (MIC=0.09 mg/ml) among the bacteria, and by Phialophora fastigiata (FSB81), (MIC=1.95 mg/ml) among the fungi. The composition of H. sendtneri extracts was also determined using HPLC analysis. Rosmarinic acid was found to be the dominant phenolic compound. The Allium anaphase-telophase genotoxicity assay revealed that the ethanolic extract of H. sendtneri at concentrations of 31.5 mg/l and below does not produce toxic or genotoxic effects. This is the first report of chemical constituents, genotoxic and antimicrobial activities of the endemic species, H.  sendtneri.",
publisher = "Warsaw: Versita",
journal = "Central European Journal of Biology",
title = "Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler",
number = "2",
volume = "7",
doi = "10.2478/s11535-012-0021-8",
pages = "327-333"
}

Mašković, P., Dragišić Maksimović, J., Maksimović, V., Blagojević, J., Vujošević, M., Manojlović, N. T., Radojković, M., Cvijović, M.,& Solujić, S.. (2012). Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler. in Central European Journal of Biology
Warsaw: Versita., 7(2), 327-333.
https://doi.org/10.2478/s11535-012-0021-8

Mašković P, Dragišić Maksimović J, Maksimović V, Blagojević J, Vujošević M, Manojlović NT, Radojković M, Cvijović M, Solujić S. Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler. in Central European Journal of Biology. 2012;7(2):327-333.
doi:10.2478/s11535-012-0021-8 .

Mašković, Pavle, Dragišić Maksimović, Jelena, Maksimović, Vuk, Blagojević, Jelena, Vujošević, Mladen, Manojlović, Nedeljko T., Radojković, Marija, Cvijović, Milica, Solujić, Slavica, "Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler" in Central European Journal of Biology, 7, no. 2 (2012):327-333,
https://doi.org/10.2478/s11535-012-0021-8 . .

TY  - JOUR
AU  - Živković, Suzana
AU  - Popović, M.
AU  - Dragišić-Maksimović, Jelena
AU  - Momčilović, Ivana
AU  - Grubišić, D.
PY  - 2010
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/266
AB  - Asplenium ceterach belongs to a group of poikilohydric ferns and it can recover uninjured from an almost completely dehydrated state. In our study, short term dehydration (24h) at four different water potentials, resulted in moderate water loss (partial desiccation) in fern tissue. The main phenolic acids represented in A. ceterach were chlorogenic (CGA) and caffeic acid (CA) and their content decreased during the dehydration process. For the first time, peroxidase (POD) and polyphenol oxidase (PPO) isoforms were determined in the rustyback fern. The results exhibit the presence of numerous anionic POD isoforms, with pI ranging from 4.4 to 5.8, but none of the cationic isoforms was detected. Two PPO isoforms were identified, one anionic with pI 6.3 and one cationic with pI of about 9.0. Short-term dehydration brought about a remarkable increase in POD and PPO activity using CGA as a substrate. Changes in enzyme activity and content of substrates during dehydration may play an important role in the adaptation of the rustyback fern to water deficit, and increase the overall plant resistance to stress conditions.
T2  - Archives of Biological Sciences
T1  - Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern Asplenium ceterach L.
IS  - 4
VL  - 62
SP  - 1071
EP  - 1081
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_266
ER  - 

@article{
author = "Živković, Suzana and Popović, M. and Dragišić-Maksimović, Jelena and Momčilović, Ivana and Grubišić, D.",
year = "2010, 2010",
abstract = "Asplenium ceterach belongs to a group of poikilohydric ferns and it can recover uninjured from an almost completely dehydrated state. In our study, short term dehydration (24h) at four different water potentials, resulted in moderate water loss (partial desiccation) in fern tissue. The main phenolic acids represented in A. ceterach were chlorogenic (CGA) and caffeic acid (CA) and their content decreased during the dehydration process. For the first time, peroxidase (POD) and polyphenol oxidase (PPO) isoforms were determined in the rustyback fern. The results exhibit the presence of numerous anionic POD isoforms, with pI ranging from 4.4 to 5.8, but none of the cationic isoforms was detected. Two PPO isoforms were identified, one anionic with pI 6.3 and one cationic with pI of about 9.0. Short-term dehydration brought about a remarkable increase in POD and PPO activity using CGA as a substrate. Changes in enzyme activity and content of substrates during dehydration may play an important role in the adaptation of the rustyback fern to water deficit, and increase the overall plant resistance to stress conditions.",
journal = "Archives of Biological Sciences",
title = "Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern Asplenium ceterach L.",
number = "4",
volume = "62",
pages = "1071-1081",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_266"
}

Živković, S., Popović, M., Dragišić-Maksimović, J., Momčilović, I.,& Grubišić, D.. (2010). Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern Asplenium ceterach L.. in Archives of Biological Sciences, 62(4), 1071-1081.
https://hdl.handle.net/21.15107/rcub_ibiss_266

Živković S, Popović M, Dragišić-Maksimović J, Momčilović I, Grubišić D. Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern Asplenium ceterach L.. in Archives of Biological Sciences. 2010;62(4):1071-1081.
https://hdl.handle.net/21.15107/rcub_ibiss_266 .

Živković, Suzana, Popović, M., Dragišić-Maksimović, Jelena, Momčilović, Ivana, Grubišić, D., "Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern Asplenium ceterach L." in Archives of Biological Sciences, 62, no. 4 (2010):1071-1081,
https://hdl.handle.net/21.15107/rcub_ibiss_266 .