@article{
author = "Brisevac, Dusica and Adzic, Marija and Laketa, Danijela and Parabucki, Ana and Milosevic, Milena and Lavrnja, Irena and Bjelobaba, Ivana and Sevigny, Jean and Kipp, Markus and Nedeljkovic, Nadezda",
year = "2015",
abstract = "Extracellular ATP (eATP) acts as a danger-associated molecular pattern
which induces reactive response of astrocytes after brain insult,
including morphological remodeling of astrocytes, proliferation,
chemotaxis, and release of proinflammatory cytokines. The responses
induced by eATP are under control of ecto-nucleotidases, which catalyze
sequential hydrolysis of ATP to adenosine. In the mammalian brain,
ecto-nucleotidases comprise three enzyme families: ecto-nucleoside
triphosphate diphosphohydrolases 1-3 (NTPDase1-3), ecto-nucleotide
pyrophosphatase/phospodiesterases 1-3 (NPP1-3), and ecto-5'-nucleotidase
(eN), which crucially determine ATP/adenosine ratio in the pericellular
milieu. Altered expression of ecto-nucleotidases has been demonstrated
in several experimental models of human brain dysfunctions. In the
present study, we have explored the pattern of NTPDase1-3, NPP1-3, and
eN expression by cultured cortical astrocytes challenged with 1 mmol/L
ATP (eATP). At the transcriptional level, eATP upregulated expression of
NTPDase1, NTPDase2, NPP2, and eN, while, at translational and functional
levels, these were paralleled only by the induction of NTPDase2 and eN.
Additionally, eATP altered membrane topology of eN, from clusters
localized in membrane domains to continuous distribution along the cell
membrane. Our results suggest that eATP, by upregulating NTPDase2 and eN
and altering the enzyme membrane topology, affects local kinetics of ATP
metabolism and signal transduction that may have important roles in the
process related to inflammation and reactive gliosis.",
journal = "Journal of Molecular Neuroscience",
title = "Extracellular ATP Selectively Upregulates Ecto-Nucleoside Triphosphate
Diphosphohydrolase 2 and Ecto-5'-Nucleotidase by Rat Cortical Astrocytes
In Vitro",
number = "3",
volume = "57",
doi = "10.1007/s12031-015-0601-y",
pages = "452-462"
}